KR100213645B1 - Process for by product ferment lysine - Google Patents

Abstract

The present invention relates to a method for treating fermentation by-products arising from lysine fermentation, and more particularly, to the concentration of by-products while separating by the concentration method using the solubility of sulfate, that is, yuan and potassium sulfate, present in the fermentation by-products. To a concentration of 50% (W / W) to 70% (W / W) to use as a feed source for livestock.

Accordingly, an object of the present invention is to provide a method for separating and concentrating an inorganic salt in excess of lysine fermentation by-product and using it as a feed source for livestock.

In other words, the present invention is dehydrated and separated by the concentration method using the solubility of the sulfate contained in the lysine fermentation by-products to lower the sulfur content from 7.5% (W / W) to 1.5% (W / W), fermentation by-products The concentration of 50% (W / W) to 70% (W / W) is characterized by using as a feed source for livestock.

Lysine fermentation by-product from which the sulfate is removed according to the present invention can be used as a livestock feed source.

Description

Treatment method of lysine fermentation by-product and method of using it in blended feed

The present invention relates to a method for treating fermentation by-products arising from lysine fermentation, and more specifically, to the concentration of by-products while separating by the concentration method using the solubility of sulfate, that is, yuan and potassium sulfate in excess in the fermentation by-products. To a concentration of 50% (W / W) to 70% (W / W) to use as a feed source for livestock.

Fermentation industry generates a large amount of fermentation by-products, lysine fermentation by-products referred to in the present invention is a lysine fermentation after separating the microbial cells from the fermentation broth, the lysine is fixed in the ion exchange resin and the remaining fermentation broth solid content 50% The concentrated solution is named as (W / W).

Lysine fermentation by-products have a high moisture content and contain various kinds of inorganic salts derived from materials used in fermentation and process additives, which makes it difficult to process and recycle them. Recycling of lysine fermentation by-products, and more specifically, the use of livestock as a feed source, has been studied for many years (K, Hannon, A. Trenkle, J. Anim. Sci. 68: 2634 (1990); S. Latos, A). Anddor, 45th Meeting of EAAP (1994) This by-product has high nitrogen and carbohydrate content and contains various organic acids and amino acids as nutrients for livestock produced during fermentation, and has been promoted as a feed source for livestock. The biggest problem in using fermented by-products as feed source is the inorganic salts contained in the by-products, among which ammonium ions, potassium ions and sulfate ions, which are cations. It not only inhibits water evaporation during concentration, but also restricts the concentration of by-products due to work disruptions due to scale generation in the facility, etc. If the moisture content in the feed source is high, the amount of additives in the production of formulated feed is limited. Limited by the amount that can be used as the source, so the moisture content of formulated feed are limited to less than 13%, a problem occurs mold.

The nutritional problem caused by inorganic salts is sulfur content provided by sulfate ion, but inorganic sulfur, which is not organic, shows severe toxicity when ingested, thus limiting the sulfur content of livestock feed. For example, the US standard suggests that the amount of sulfur that can be contained in cattle feed is 0.1%. National Research Council, 1984. Nutrient Requirements of Beef Cattle

The problem in practical use is the precipitation of sulphate liquid in high content of liquid. In the storage tank of feed mill, salt is deposited at the bottom part and it is difficult to uniform feed mixing if it is not stirred continuously. Difficulty in using such a phenomenon is also caused.

The present inventors firstly, to solve the above problems, first, to what extent to remove more than 7.5% (w / w) of sulfur contained in the fermentation by-products. Second, the sulfur content was 7.5% (w / w) during the study, focusing on the fact that the moisture content as a liquid raw material was not too high and the liquidity problem, which is a disadvantage of the highly concentrated liquid raw material, should be maintained. The present invention was completed by finding that the concentration of the liquid at 1.5% (w / w) at 50% (w / w) to 70% (w / w) was most suitable as a feed source for livestock.

Accordingly, it is an object of the present invention to provide a method for separating and concentrating an inorganic salt present in the lysine fermentation by-product and using it as a feed source for livestock.

That is, in the present invention, the crystal content is dehydrated and separated by the concentration method using the dissolution concentration of sulfate contained in the lysine fermentation by-product, and the sulfur content is reduced from 7.5% (w / w) to 1.5% (w / w), and the fermentation by-product is The concentration of 50% (w / w) to 70% (w / w) is characterized by using as a feed source for livestock.

1 is a process flow diagram of a method according to the invention.

In the method for treating sulfate contained in lysine fermentation by-products, first, by-product concentration of 50% (w / w) is concentrated to 60% (w / w), and ammonia ion, potassium ion, and sulfate ion depending on the solubility Precipitates with potassium sulfate. The precipitated sulphate is dehydrated and separated, and the remaining primary mother liquor is concentrated again to a concentration of 70% (w / w) to precipitate sulphate crystals again. Secondly, the precipitated sulphate is separated into a slurry using Super Decanter. Sulfate is separated twice and 2-5% (w / w) of the remaining mother liquor is added to the blended feed and used as feed source. This method can increase the concentration of organic matter in the liquid depending on the degree of sulphate removal, and determine the degree of sulphate removal depending on the concentration.

The present invention will be described in more detail with reference to the following examples.

Example 1

As a main raw material, sugar cane molasses was cultured by the lysine producing strain to produce lysine, and the result of analysis of the fermentation by-product component of 50% (w / w) remaining after recovering lysine from the fermentation broth is shown in Table 1 below.

Example 2

The lysine fermentation by-product obtained in Example 1 was continuously precipitated with sulfate crystals in the form of phosphate and potassium sulfate in a continuous condenser at a temperature of 60-80 ° C. and a concentration of 70-80% (w / w). The precipitated crystal solution and the primary crystal were obtained as shown in Table 2.

Example 3

The primary mother liquor obtained in Example 2 was concentrated under conditions of a temperature of 70-90 ° C. and a concentration of 70-85% (w / w) in a continuous single concentration tube to precipitate sulfate as fine slurry particles. Since this crystal solution was very high in viscosity and solid-liquid separation was not possible with a general centrifugal dehydrator, the solution and the slurry were separated using Super Decanter. The obtained salt slurry was recrystallized by mixing with the fermentation by-product of Example 2, the secondary mother liquor of the concentration of 70% (w / w) was used as a feed source. Component changes due to the second concentrated dehydration are shown in Table 3.

Example 4

Beef growth experiment was conducted for 8 weeks using the salt removal fermentation by-product prepared in Example 3. The control group used corn, protein peel, wheat buckwheat, rapeseed gourd, cottonseed gourd, molasses, and the like as the main ingredients, and the by-product was fed by replacing the molasses at the level of 5% (w / w). The growth results are shown in Table 4.

If lysine fermentation by-products are used at a compound feed level of 5% (w / w), a feed source containing less than 1.5% (W / W) of sulfur content should be made in accordance with the sulfur content of the animal feed. The raw material with the highest viscosity among the liquid raw materials used in the feed is molasses, and has a viscosity of about 10,000-20,000 cps. Therefore, the raw material having a viscosity higher than that is inconvenient to use as a feed source. In the present invention, the lysine fermentation broth shows 5,000-10,000 cps at a concentration of 70% (w / w), so there is no problem in use as a feed source.

In addition, the lysine fermentation by-products from which the sulphate was removed according to the present invention had a slightly better feed efficiency when molasses and fertilizer were used as livestock feed sources as shown in the beef cattle growth test results of Table 4, but there was almost no difference in weight gain. Can be used.

Claims (3)

In preparing feedstock for livestock by removing inorganic salts from lysine fermentation by-products, the concentration of fermentation by-products of sulfur content of 7.5% (w / w) and 50% (w / w) of solids is concentrated, so A method for treating lysine fermentation by-products, characterized in that the precipitate is precipitated, and the precipitated sulfate crystal is dehydrated and separated to obtain a sulfur content of 1.5% (w / w) and a solid content of 70% (w / w). The method for treating lysine fermentation by-products according to claim 1, wherein the inorganic salts are ammonium sulfate and potassium sulfate, and the sulfur salts are lowered using their respective solubility differences. A method of adding 1-5% (w / w) of mother liquor obtained by treating the lysine fermentation by-product according to the method of claim 1 to use in blended feed.