KR0163513B1 - Prominent cosmetic compositions in effects preventing skin aging - Google Patents
Prominent cosmetic compositions in effects preventing skin aging Download PDFInfo
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- KR0163513B1 KR0163513B1 KR1019950066680A KR19950066680A KR0163513B1 KR 0163513 B1 KR0163513 B1 KR 0163513B1 KR 1019950066680 A KR1019950066680 A KR 1019950066680A KR 19950066680 A KR19950066680 A KR 19950066680A KR 0163513 B1 KR0163513 B1 KR 0163513B1
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- hydroxyquinoline
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- cosmetic compositions
- preventing skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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Abstract
본 발명은 피부노화 억제효과가 우수한 화장료 조성물에 관한 것으로서, 8-하이드록시퀴놀린을 0.01-10중량% 함유하는 것을 특징으로 한다.The present invention relates to a cosmetic composition having an excellent skin aging inhibitory effect, characterized in that it contains 0.01-10% by weight of 8-hydroxyquinoline.
Description
본 발명은 8-하이드록시퀴놀린(8-Hydroxyquinoline, Oxine)을 함유하므로써 피부노화 억제효과가 우수한 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition excellent in inhibiting skin aging by containing 8-hydroxyquinoline (8-Hydroxyquinoline, Oxine).
일반적으로 피부노화는 체내 및 체외의 여러가지 요인에 의해 생성되는 프리 라디칼(free radical)에 의해서 발생된다고 하는 이론이 정설로 받아들여지고 있다. 이러한 프리 라디칼들은 반응식으로 표시되는 다단계 연쇄반응을 통하여 증폭되며 이러한 반응 결과 생성되는 과산화 지질들이 장시간 축척되고, 이와 동시에 프리 라디칼들의 조직내의 여러 거대분자들과 무작위적인 반응을 통하여, 가교결합을 형성하게 된다(Barry Halliwell, John M.C. Gutteridge, Biochem:ISI ATLAS of science, 48-52. Giorgio Minetti, Steven D. Aust, J. biological Chemistry Vol 262, 1098-1104).In general, the theory that skin aging is caused by free radicals generated by various factors in and out of the body is accepted as the orthodoxy. These free radicals are amplified through a multistage chain reaction represented by the reaction scheme and the resulting lipid peroxides accumulate for a long time, and at the same time, random reactions with various macromolecules in the tissue of the free radicals form crosslinks. (Barry Halliwell, John MC Gutteridge, Biochem: ISI ATLAS of science, 48-52. Giorgio Minetti, Steven D. Aust, J. biological Chemistry Vol 262, 1098-1104).
따라서, 상기한 프리라디칼의 연쇄반응에 의해서 축적되는 과산화 지질들에 의해서 피부노화가 일어나는데, 이러한 연쇄반응에 관여하는 철 이온 또는 구리 이온을 킬레이션(Chelation)하므로써 프리라디칼의 연쇄반응을 정지시켜 결과적으로 피부노화를 억제시킬 수 있다.Therefore, skin aging is caused by lipid peroxides accumulated by the chain reaction of the free radicals, and the chain reaction of the free radicals is stopped by chelation of iron or copper ions involved in the chain reaction. This can suppress skin aging.
본 발명자들은 철 이온이나 구리 이온을 킬레이션(chelation)하는 여러가지 물질들을 대상으로 피부노화 억제효과에 대해서 연구하던중 배양세포와 자외선 조사기를 이용한 실험에서 8-하이드록시퀴놀린(8-Hydroxyquinoline, Oxine)이 피부노화를 억제하는데 탁월한 효과가 있음을 발견하였으며, 최종적으로 실험동물을 이용한 실험에서 그 효과가 입증되어 본 발명을 완성하게 되었다.The present inventors studied the effect of inhibiting skin aging of various substances chelation of iron ions or copper ions, and 8-hydroxyquinoline (8-Hydroxyquinoline, Oxine) in experiments using cultured cells and UV irradiation. It was found that there is an excellent effect in suppressing this skin aging, and finally the effect was proved in the experiment using the experimental animal to complete the present invention.
즉, 본 발명은 8-하이드록시퀴놀린 0.01-10중량%, 바람직하게는 0.05중량% 함유함을 특징으로 한다. 0.01중량% 이하의 농도에서는 피부노화 억제 효과를 기대할 수 없었으며, 10중량% 이상의 농도에서는 함량의 증가에 따라 뚜럿한 효과의 증가가 나타나지 않았다.That is, the present invention is characterized by containing 0.01-10% by weight, preferably 0.05% by weight of 8-hydroxyquinoline. At the concentration of 0.01 wt% or less, the effect of inhibiting skin aging could not be expected.
본 발명에 따른 8-하이드록시퀴놀린이 함유된 조성물은 유연 화장수, 영양화장수, 영양크림, 맛사지 크림 및 에센스 등에 작용될 수 있다.The composition containing 8-hydroxyquinoline according to the present invention can be applied to flexible lotion, nourishing cosmetics, nourishing cream, massage cream and essence.
이하 실험예 및 실시예를 통하여 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail with reference to Examples and Examples.
[실험예 1]Experimental Example 1
과산화 지질 측정법Lipid Peroxide Assay
배양세포를 이용하여 8-하이드록시퀴놀린의 피부노화 억제효과를 다음과 같이 측정하였다.The cultured cells were used to measure the skin aging inhibitory effect of 8-hydroxyquinoline as follows.
24 웰 플레이트(well plate)에 배양된 사람의 섬유아세포를 접종하여 배양용기의 바닥에 단층을 형성시킨 후, 크산틴-크산틴 옥시다아제(Xanthine-Xanthine oxidase) 및 여러 농도의 단층을 형성시킨 후, 크산틴-크산틴 옥시다아제(Xanthine-Xanthine oxidase) 및 여러 농도의 8-하이드록시퀴놀린을 첨가하여, 24시간 동안 배양한 후 세포를 떼어내어 과산화 지질을 정량하고, 과산화 지질의 생성량으로 피부노화억제 정도를 평가하였다(International J. Cosmetic Science 12, 105-114, 1990).After inoculating human fibroblasts in a 24-well plate to form a monolayer at the bottom of the culture vessel, and then forming a xanthine-Xanthine oxidase and monolayers of various concentrations, Xanthine-Xanthine oxidase and various concentrations of 8-hydroxyquinoline were added, and after culturing for 24 hours, cells were detached to quantify lipid peroxide, and the amount of lipid peroxide inhibited skin aging. Were evaluated (International J. Cosmetic Science 12, 105-114, 1990).
대조군으로는 크산틴-크산틴 옥시다아제(Xanthine-Xanthine oxidase)만 포함하는 대조군(+)과 아무것도 포함하지 않는 대조군(-)을 사용하여 피부노화억제효과를 비교하였으며, 그 결과는 하기표 1과 같다.As a control group, the skin aging inhibitory effect was compared by using a control group (+) containing only xanthine-Xanthine oxidase and a control group (-) containing nothing, and the results are shown in Table 1 below. .
이 때, 세포배양에 필요한 시약들은 지브코(Gibco)사의 것과 시그마(Sigma)사의 것을 사용하였으며, 24 웰 플레이트(well plate)는 코닝(Coning)사의 것을 사용하였다.At this time, the reagents required for cell culture were used by Gibco Co., Ltd. and Sigma Co., Ltd., and 24 well plates were used by Corning Co., Ltd.
상기 표1의 결과에서 알 수 있듯이, 8-하이드록시퀴놀린의 농도가 증가함에 따라 과산화지질의 생성량은 감소하였으며, 대조군(+)과 비교했을 때 과산화 지질 생성억제효과가 우수함을 알 수 있다. 이는 8-하이드록시퀴놀린이 피부노화 억제효과가 크다는 것을 입증하는 것이다.As can be seen from the results of Table 1, as the concentration of 8-hydroxyquinoline increases, the amount of lipid peroxide decreases, and it can be seen that the lipid peroxide production inhibitory effect is excellent compared to the control (+). This demonstrates that 8-hydroxyquinoline has a great anti-aging effect.
[실험예 2]Experimental Example 2
ODC 활성 저해효과 측정Inhibition of ODC Activity
24 웰 플레이트 각각의 웰에 5×10 세포를 접종하여 단층을 형성시킨 뒤 각각의 웰에 0.3%의 8-MOP와 적당한 농도의 8-하이드록시퀴놀린을 투여하고 3J/Cm 의 자외선(UV-A)을 조사한후 24시간 동안 배양한 뒤 오르니틴 디카르복시아제(Ornithine Decarboxyase, 이하 ODS라 한다)의 활성도를 방사성 동위원소 실험법을 이용하여 측정하였다(T.G.O' Brien, Cancer Research 36, 2644-2653, 1976).5 × 10 in each well of a 24 well plate Cells were seeded to form a monolayer, and then each well was administered with 0.3% 8-MOP and appropriate concentration of 8-hydroxyquinoline and 3J / Cm After irradiated with ultraviolet (UV-A) incubated for 24 hours, the activity of ornithine decarboxyase (hereinafter referred to as ODS) was measured by radioisotope assay (TGO 'Brien, Cancer Research 36 , 2644-2653, 1976).
상기 표 2의 결과로부터, 8-하이드록시퀴놀린은 자외선이 조사된 배양된 사람 세포의 CDC 활성의 증가를 효과적으로 억제하고 있음을 알 수 있었다.From the results of Table 2, it can be seen that 8-hydroxyquinoline effectively inhibits the increase of CDC activity of cultured human cells irradiated with ultraviolet light.
이와 같이 피부노화 억제효과가 우수한 8-하이드록시퀴놀린을 이용하여 다음의 실시예와 같이 화장료를 제조하였다. 그러나 본 발명은 다음의 실시예에만 국한되는 것은 아님을 밝혀둔다.Thus, using the 8-hydroxyquinoline excellent skin aging inhibitory effect was prepared a cosmetic as in the following examples. However, it should be noted that the present invention is not limited to the following examples.
[실시예 1]Example 1
유연화장수Flexible Cosmetics
하기 표3의 조성에 따라, 통상의 방법으로 유연 화장수를 제조하였다.According to the composition of Table 3, to prepare a flexible lotion in a conventional manner.
[실시예 2]Example 2
영양화장수Nutrition
하기 표 4의 조성에 따라, 통상의 방법으로 영양 화장수를 제조하였다.According to the composition of Table 4, the nutritional lotion was prepared by a conventional method.
[실시예 3]Example 3
영양크림Nutrition Cream
하기 표 5의 조성에 따라, 통상의 방법으로 영양크림을 제조하였다.According to the composition of Table 5, nutrition cream was prepared in a conventional manner.
[실험예 3]Experimental Example 3
실시예 3에 따라 제조된 화장료를 생후 8주령의 헤어리스 마우스(Hairless mouse)의 등부위에 1일 2회, 4주간 적량 도포한 후 자외선을 조사하여 아무것도 적용하지 않은 비교군에서 측정된 SPF 지수와 비교하였다. 각각의 실험군에 사용된 마우스의 수는 10마리씩 총 80마리를 사용하여 실험하였으며, 그 결과를 하기 표 6에 나타내었다.After applying the cosmetics prepared according to Example 3 to the back of 8-week-old hairless mouse twice a day for 4 weeks, and then irradiated with ultraviolet rays, the SPF index measured in the comparison group was not applied. Compared. The number of mice used in each experimental group was tested using a total of 80 animals, each 10, the results are shown in Table 6 below.
상기 표 6의 결과로부터, 본 발명에 따른 화장료를 도포한 10마리의 실험동물 모두가 자외선에 의한 SPF 지수 측정에서 현저한 효과를 나타냄으로써, 8-하이드록시퀴놀린 함유 화장료의 피부노화 억제효과를 입증하였다.From the results in Table 6, all 10 experimental animals coated with a cosmetic according to the present invention exhibited a remarkable effect in measuring the SPF index by UV light, thereby demonstrating the skin aging inhibitory effect of 8-hydroxyquinoline-containing cosmetics. .
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| Application Number | Priority Date | Filing Date | Title |
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| KR1019950066680A KR0163513B1 (en) | 1995-12-29 | 1995-12-29 | Prominent cosmetic compositions in effects preventing skin aging |
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| KR1019950066680A KR0163513B1 (en) | 1995-12-29 | 1995-12-29 | Prominent cosmetic compositions in effects preventing skin aging |
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| Publication Number | Publication Date |
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| KR970032836A KR970032836A (en) | 1997-07-22 |
| KR0163513B1 true KR0163513B1 (en) | 1998-12-01 |
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