JPWO2023054573A1 - - Google Patents
Info
- Publication number
- JPWO2023054573A1 JPWO2023054573A1 JP2023551836A JP2023551836A JPWO2023054573A1 JP WO2023054573 A1 JPWO2023054573 A1 JP WO2023054573A1 JP 2023551836 A JP2023551836 A JP 2023551836A JP 2023551836 A JP2023551836 A JP 2023551836A JP WO2023054573 A1 JPWO2023054573 A1 JP WO2023054573A1
- Authority
- JP
- Japan
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021161312 | 2021-09-30 | ||
| JP2021161312 | 2021-09-30 | ||
| PCT/JP2022/036403 WO2023054573A1 (ja) | 2021-09-30 | 2022-09-29 | 相同染色体の一方に特異的なｄｎａ欠失を有する細胞を製造する方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPWO2023054573A1 true JPWO2023054573A1 (https=) | 2023-04-06 |
| JP7672012B2 JP7672012B2 (ja) | 2025-05-07 |
Family
ID=85782881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023551836A Active JP7672012B2 (ja) | 2021-09-30 | 2022-09-29 | 相同染色体の一方に特異的なｄｎａ欠失を有する細胞を製造する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20250129390A1 (https=) |
| EP (1) | EP4410975A4 (https=) |
| JP (1) | JP7672012B2 (https=) |
| CN (1) | CN118043456A (https=) |
| WO (1) | WO2023054573A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024204215A1 (ja) * | 2023-03-28 | 2024-10-03 | 国立大学法人大阪大学 | 相同染色体の一方のＣｏｌ７ａ遺伝子に特異的なＤＮＡ欠失を有する細胞を製造する方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016520317A (ja) * | 2013-05-29 | 2016-07-14 | セレクティスＣｅｌｌｅｃｔｉｓ | Ｃａｓ９ニッカーゼ活性を用いて正確なｄｎａ切断をもたらすための方法 |
| JP2017535271A (ja) * | 2014-11-21 | 2017-11-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ガイドｒｎａのペアを使用したターゲティングによる遺伝子改変の方法及び組成物 |
| JP2018011525A (ja) * | 2016-07-19 | 2018-01-25 | 国立大学法人大阪大学 | ゲノム編集方法 |
| WO2020100361A1 (ja) * | 2018-11-16 | 2020-05-22 | 国立大学法人大阪大学 | ゲノム編集された細胞を製造する方法 |
| JP2021519067A (ja) * | 2018-03-23 | 2021-08-10 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 常染色体優性疾患のための遺伝子編集 |
| WO2021163556A1 (en) * | 2020-02-12 | 2021-08-19 | The Children's Hospital Of Philadelphia | Compositions and methods for inducible alternative splicing regulation of gene expression |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2922393B2 (en) | 2013-02-27 | 2022-12-28 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Gene editing in the oocyte by cas9 nucleases |
-
2022
- 2022-09-29 US US18/696,121 patent/US20250129390A1/en active Pending
- 2022-09-29 WO PCT/JP2022/036403 patent/WO2023054573A1/ja not_active Ceased
- 2022-09-29 EP EP22876437.9A patent/EP4410975A4/en not_active Withdrawn
- 2022-09-29 CN CN202280065806.1A patent/CN118043456A/zh active Pending
- 2022-09-29 JP JP2023551836A patent/JP7672012B2/ja active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016520317A (ja) * | 2013-05-29 | 2016-07-14 | セレクティスＣｅｌｌｅｃｔｉｓ | Ｃａｓ９ニッカーゼ活性を用いて正確なｄｎａ切断をもたらすための方法 |
| JP2017535271A (ja) * | 2014-11-21 | 2017-11-30 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ガイドｒｎａのペアを使用したターゲティングによる遺伝子改変の方法及び組成物 |
| JP2018011525A (ja) * | 2016-07-19 | 2018-01-25 | 国立大学法人大阪大学 | ゲノム編集方法 |
| JP2021519067A (ja) * | 2018-03-23 | 2021-08-10 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 常染色体優性疾患のための遺伝子編集 |
| WO2020100361A1 (ja) * | 2018-11-16 | 2020-05-22 | 国立大学法人大阪大学 | ゲノム編集された細胞を製造する方法 |
| WO2021163556A1 (en) * | 2020-02-12 | 2021-08-19 | The Children's Hospital Of Philadelphia | Compositions and methods for inducible alternative splicing regulation of gene expression |
Non-Patent Citations (1)
| Title |
|---|
| 中田慎一郎: "Cas9変異体によるニックが起こす相同組換え修復様DNA組換えの分子機構の解明", 科学研究費助成事業 研究成果報告書, ［ONLINE］, JPN6024047626, 30 March 2020 (2020-03-30), ISSN: 0005469810 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20250129390A1 (en) | 2025-04-24 |
| JP7672012B2 (ja) | 2025-05-07 |
| WO2023054573A1 (ja) | 2023-04-06 |
| EP4410975A1 (en) | 2024-08-07 |
| CN118043456A (zh) | 2024-05-14 |
| EP4410975A4 (en) | 2025-01-01 |
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