JPWO2022115810A5 - - Google Patents
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- Publication number
- JPWO2022115810A5 JPWO2022115810A5 JP2023532507A JP2023532507A JPWO2022115810A5 JP WO2022115810 A5 JPWO2022115810 A5 JP WO2022115810A5 JP 2023532507 A JP2023532507 A JP 2023532507A JP 2023532507 A JP2023532507 A JP 2023532507A JP WO2022115810 A5 JPWO2022115810 A5 JP WO2022115810A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleobase
- dna
- sample
- aliquot
- borane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004414 DNA Proteins 0.000 claims description 129
- 238000000034 method Methods 0.000 claims description 127
- 239000000523 sample Substances 0.000 claims description 72
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 35
- UORVGPXVDQYIDP-BJUDXGSMSA-N borane Chemical class [10BH3] UORVGPXVDQYIDP-BJUDXGSMSA-N 0.000 claims description 24
- 229910000085 borane Inorganic materials 0.000 claims description 24
- 239000003638 chemical reducing agent Substances 0.000 claims description 24
- UORVGPXVDQYIDP-UHFFFAOYSA-N trihydridoboron Substances B UORVGPXVDQYIDP-UHFFFAOYSA-N 0.000 claims description 24
- 230000001973 epigenetic effect Effects 0.000 claims description 20
- 238000012163 sequencing technique Methods 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 230000011987 methylation Effects 0.000 claims description 17
- 238000007069 methylation reaction Methods 0.000 claims description 17
- 238000000638 solvent extraction Methods 0.000 claims description 16
- QHXLIQMGIGEHJP-UHFFFAOYSA-N boron;2-methylpyridine Chemical group [B].CC1=CC=CC=N1 QHXLIQMGIGEHJP-UHFFFAOYSA-N 0.000 claims description 15
- NNTOJPXOCKCMKR-UHFFFAOYSA-N boron;pyridine Chemical compound [B].C1=CC=NC=C1 NNTOJPXOCKCMKR-UHFFFAOYSA-N 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 102000004169 proteins and genes Human genes 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- 229940104302 cytosine Drugs 0.000 claims description 10
- 230000004048 modification Effects 0.000 claims description 10
- 238000012986 modification Methods 0.000 claims description 10
- JBANFLSTOJPTFW-UHFFFAOYSA-N azane;boron Chemical compound [B].N JBANFLSTOJPTFW-UHFFFAOYSA-N 0.000 claims description 9
- GKFJEDWZQZKYHV-UHFFFAOYSA-N borane;2-methylpropan-2-amine Chemical compound B.CC(C)(C)N GKFJEDWZQZKYHV-UHFFFAOYSA-N 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 8
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 claims description 7
- RYVNIFSIEDRLSJ-UHFFFAOYSA-N 5-(hydroxymethyl)cytosine Chemical compound NC=1NC(=O)N=CC=1CO RYVNIFSIEDRLSJ-UHFFFAOYSA-N 0.000 claims description 6
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 6
- 238000011282 treatment Methods 0.000 claims description 6
- 230000009615 deamination Effects 0.000 claims description 5
- 238000006481 deamination reaction Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 4
- 108010014064 CCCTC-Binding Factor Proteins 0.000 claims description 2
- 102000016897 CCCTC-Binding Factor Human genes 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 108010033040 Histones Proteins 0.000 claims description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 claims description 2
- 108091005646 acetylated proteins Proteins 0.000 claims description 2
- 230000003321 amplification Effects 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 238000001574 biopsy Methods 0.000 claims description 2
- 230000013595 glycosylation Effects 0.000 claims description 2
- 238000006206 glycosylation reaction Methods 0.000 claims description 2
- 238000001114 immunoprecipitation Methods 0.000 claims description 2
- 108091005592 methylated proteins Proteins 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 238000011176 pooling Methods 0.000 claims description 2
- 206010028980 Neoplasm Diseases 0.000 description 22
- 201000011510 cancer Diseases 0.000 description 22
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical class N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 4
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- 230000004075 alteration Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063119520P | 2020-11-30 | 2020-11-30 | |
| US63/119,520 | 2020-11-30 | ||
| PCT/US2021/061280 WO2022115810A1 (en) | 2020-11-30 | 2021-11-30 | Compositions and methods for enriching methylated polynucleotides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023551292A JP2023551292A (ja) | 2023-12-07 |
| JP2023551292A5 JP2023551292A5 (https=) | 2024-11-21 |
| JPWO2022115810A5 true JPWO2022115810A5 (https=) | 2024-11-21 |
Family
ID=79164721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023532507A Pending JP2023551292A (ja) | 2020-11-30 | 2021-11-30 | メチル化されたポリヌクレオチドを富化するための組成物および方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20240150844A1 (https=) |
| EP (1) | EP4251765A1 (https=) |
| JP (1) | JP2023551292A (https=) |
| CA (1) | CA3199829A1 (https=) |
| WO (1) | WO2022115810A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2024512156A (ja) * | 2021-03-30 | 2024-03-18 | レゾリューション バイオサイエンス， インコーポレイテッド | 複数のライブラリーを同時に遺伝子解析するための組成物及び方法 |
| EP4655416A1 (en) * | 2023-01-25 | 2025-12-03 | Guardant Health, Inc. | Nucleic acid methylation profiling method |
| WO2025090956A1 (en) * | 2023-10-26 | 2025-05-01 | Guardant Health, Inc. | Methods for detecting nucleic acid variants using capture probes |
| WO2025155895A1 (en) * | 2024-01-19 | 2025-07-24 | Guardant Health, Inc. | Nucleic acid modification profiling method |
| GB2644427A (en) * | 2024-07-23 | 2026-04-15 | Tagomics Ltd | Profiling method |
| WO2026024692A1 (en) * | 2024-07-24 | 2026-01-29 | Guardant Health, Inc. | Direct methylation single-base resolution sequencing of enriched unmethylated dna |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US20030017081A1 (en) | 1994-02-10 | 2003-01-23 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| AU2002359522A1 (en) | 2001-11-28 | 2003-06-10 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| CA2519860C (en) | 2003-03-21 | 2018-01-16 | Santaris Pharma A/S | Short interfering rna (sirna) analogues |
| US8486630B2 (en) | 2008-11-07 | 2013-07-16 | Industrial Technology Research Institute | Methods for accurate sequence data and modified base position determination |
| US20110237444A1 (en) * | 2009-11-20 | 2011-09-29 | Life Technologies Corporation | Methods of mapping genomic methylation patterns |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| EP2630257B1 (en) * | 2010-10-22 | 2017-08-02 | Oslo Universitetssykehus HF | Methods and kits for detection of 5-hydroxymethylcytosine |
| GB2533006B (en) | 2012-09-04 | 2017-06-07 | Guardant Health Inc | Systems and methods to detect copy number variation |
| CN107002122B (zh) | 2014-07-25 | 2023-09-19 | 华盛顿大学 | 确定导致无细胞dna的产生的组织和/或细胞类型的方法以及使用其鉴定疾病或紊乱的方法 |
| MX391039B (es) * | 2016-04-07 | 2025-03-21 | Univ Leland Stanford Junior | Diagnostico no invasivo por medio de la secuenciacion del adn libre de celulas 5-hidroxi-metilado. |
| KR102610098B1 (ko) | 2016-07-06 | 2023-12-04 | 가던트 헬쓰, 인크. | 무세포 핵산의 프래그멘톰 프로파일링을 위한 방법 |
| US9850523B1 (en) | 2016-09-30 | 2017-12-26 | Guardant Health, Inc. | Methods for multi-resolution analysis of cell-free nucleic acids |
| CA3046007A1 (en) | 2016-12-22 | 2018-06-28 | Guardant Health, Inc. | Methods and systems for analyzing nucleic acid molecules |
| WO2019160994A1 (en) * | 2018-02-14 | 2019-08-22 | Bluestar Genomics, Inc. | Methods for the epigenetic analysis of dna, particularly cell-free dna |
| EP4567131A3 (en) * | 2019-01-31 | 2025-09-03 | Guardant Health, Inc. | Compositions and methods for isolating cell-free dna |
-
2021
- 2021-11-30 EP EP21835046.0A patent/EP4251765A1/en active Pending
- 2021-11-30 WO PCT/US2021/061280 patent/WO2022115810A1/en not_active Ceased
- 2021-11-30 CA CA3199829A patent/CA3199829A1/en active Pending
- 2021-11-30 JP JP2023532507A patent/JP2023551292A/ja active Pending
-
2023
- 2023-05-30 US US18/325,759 patent/US20240150844A1/en active Pending
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