JPWO2021255272A5 - - Google Patents

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JPWO2021255272A5
JPWO2021255272A5 JP2022578622A JP2022578622A JPWO2021255272A5 JP WO2021255272 A5 JPWO2021255272 A5 JP WO2021255272A5 JP 2022578622 A JP2022578622 A JP 2022578622A JP 2022578622 A JP2022578622 A JP 2022578622A JP WO2021255272 A5 JPWO2021255272 A5 JP WO2021255272A5
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nucleic acid
plant
acid molecule
molecule according
sequence
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Priority claimed from PCT/EP2021/066682 external-priority patent/WO2021255272A1/en
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ナス科の植物中に存在する場合、根瘤線虫に対する抵抗性を付与するMeR1タンパク質をコードする核酸分子であって、以下:
a)配列番号2、3、4、5、6、7、8または9によるコード配列、または配列番号2、3、4、5、6、7、8または9による配列と少なくとも90%の配列同一性を有するコード配列を含むヌクレオチド配列、;または
b)配列番号10、11、12、13、14、15、16または17によるアミノ酸配列を有するタンパク質、または配列番号10、11、12、13、14、15、16または17によるアミノ酸配列と少なくとも85%の配列同一性を有するアミノ酸配列を有するタンパク質をコードするヌクレオチド配列
であるヌクレオチド配列を有する、核酸分子。
1. A nucleic acid molecule encoding an MeR1 protein that confers resistance to root-knot nematodes when present in a plant of the Solanaceae family, comprising:
a) a nucleotide sequence comprising a coding sequence according to SEQ ID NO: 2, 3, 4, 5, 6, 7, 8 or 9, or a coding sequence having at least 90% sequence identity with a sequence according to SEQ ID NO: 2, 3, 4, 5, 6, 7, 8 or 9; or b) a nucleotide sequence which is a nucleotide sequence encoding a protein having an amino acid sequence according to SEQ ID NO: 10, 11, 12, 13, 14, 15, 16 or 17, or a protein having an amino acid sequence having at least 85% sequence identity with an amino acid sequence according to SEQ ID NO: 10, 11, 12, 13, 14, 15, 16 or 17.
配列番号2、3、4、5、6、7、8または9による配列との配列同一性が、優先度の高い順に91%、92%、93%、94%、95%、96%、97%、98%、99%である、請求項1に記載の核酸。 The nucleic acid according to claim 1, which has sequence identity with the sequence of SEQ ID NO: 2, 3, 4, 5, 6, 7, 8 or 9, in order of preference, of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%. 配列番号10、11、12、13、14、15、16または17によるアミノ酸配列との配列同一性が、優先度の高い順に85%、86%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%である、請求項1または請求項2に記載の核酸。 The nucleic acid according to claim 1 or 2, which has sequence identity with the amino acid sequence of SEQ ID NO: 10, 11, 12, 13, 14, 15, 16 or 17 of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, in order of preference. ナス科の植物中に請求項1~3のいずれか一項に記載の核酸分子と共に存在する場合に根瘤線虫に対する抵抗性に寄与するNRC6タンパク質をコードする核酸分子であって、ここで前記核酸分子は、以下:
a)配列番号19によるコード配列、または配列番号19による配列と少なくとも98%の配列同一性を有するコード配列を含むヌクレオチド配列;または
b)配列番号20によるアミノ酸配列を有するタンパク質、または配列番号20によるアミノ酸配列と少なくとも98%の配列同一性を有するアミノ酸配列を有するタンパク質をコードするヌクレオチド配列
であるヌクレオチド配列を有する、核酸分子。
4. A nucleic acid molecule encoding an NRC6 protein which confers resistance to root-knot nematodes when present in a plant of the Solanaceae family together with a nucleic acid molecule according to any one of claims 1 to 3, wherein said nucleic acid molecule comprises:
a) a nucleotide sequence comprising a coding sequence according to SEQ ID NO:19, or a coding sequence having at least 98% sequence identity with a sequence according to SEQ ID NO:19; or b) a nucleotide sequence which is a nucleotide sequence encoding a protein having an amino acid sequence according to SEQ ID NO:20, or a protein having an amino acid sequence having at least 98% sequence identity with an amino acid sequence according to SEQ ID NO:20.
ソラナム・リコペルシクム(Solanum lycopersicum)植物中に存在する場合に根瘤線虫に対する抵抗性を付与する、請求項1~4のいずれか一項に記載の核酸分子。 The nucleic acid molecule of any one of claims 1 to 4, which confers resistance to root-knot nematodes when present in a Solanum lycopersicum plant. 請求項1~3または5のいずれか一項に記載の核酸分子を含むナス科の植物、特にソラナム・リコペルシクム植物であって、ここで該植物は根瘤線虫に対して抵抗性である、植物。 A plant of the Solanaceae family, in particular a Solanum lycopersicum plant, comprising the nucleic acid molecule according to any one of claims 1 to 3 or 5, wherein the plant is resistant to root-knot nematodes. 請求項1~3または5のいずれか一項に記載の核酸分子および請求項4または5に記載の少なくとも1つの核酸分子を含む、ナス科の植物、特にソラナム・リコペルシクム植物であって、ここで該植物は根瘤線虫に対して抵抗性である、植物。 A plant of the Solanaceae family, in particular a Solanum lycopersicum plant, comprising a nucleic acid molecule according to any one of claims 1 to 3 or 5 and at least one nucleic acid molecule according to claim 4 or 5, wherein the plant is resistant to root-knot nematodes. 請求項1~3または5のいずれか一項に記載の核酸分子および請求項4または5に記載の2つの核酸分子を含む、ナス科の植物、特にソラナム・リコペルシクム植物であって、ここで該植物は根瘤線虫に対して抵抗性である、植物。 A plant of the Solanaceae family, in particular a Solanum lycopersicum plant, comprising a nucleic acid molecule according to any one of claims 1 to 3 or 5 and two nucleic acid molecules according to claims 4 or 5, wherein the plant is resistant to root-knot nematodes. メロイドジン・エンテロロビイ(Meloidogyne enterolobii)に対して抵抗性である、請求項6~8のいずれか一項に記載の植物。 The plant according to any one of claims 6 to 8, which is resistant to Meloidogyne enterolobii. ソラナム・リコペルシクム植物であり、ここで核酸分子が、寄託番号NCIMB 43515下でNCIMBに寄託された代表的な種子のソラナム・リコペルシクム植物のゲノムに含まれるものである、請求項8または9に記載の植物。 The plant according to claim 8 or 9, which is a Solanum lycopersicum plant, wherein the nucleic acid molecule is contained in the genome of a representative seed of the Solanum lycopersicum plant deposited with the NCIMB under the accession number NCIMB 43515. 請求項1~3または5のいずれか一項に記載の核酸分子、および所望により請求項4または5に記載の核酸分子を含む、ナス科の植物の台木。 A rootstock for a plant of the Solanaceae family, comprising a nucleic acid molecule according to any one of claims 1 to 3 or 5, and optionally a nucleic acid molecule according to claim 4 or 5. 種子から生育したナス科の植物、特にソラナム・リコペルシクム植物が、請求項1~3または5のいずれか一項に記載の核酸分子、および所望により、請求項4および5のいずれか一項に記載される核酸分子。 A plant of the Solanaceae family, in particular a Solanum lycopersicum plant, grown from seed, comprising a nucleic acid molecule according to any one of claims 1 to 3 or 5, and optionally a nucleic acid molecule according to any one of claims 4 and 5. 種子から生育したソラナム・リコペルシクム植物が、メロイドジン・エンテロロビイに対して抵抗性である、請求項11に記載の種子。 The seed according to claim 11, wherein the Solanum lycopersicum plant grown from the seed is resistant to Meloidogyne enterolobidi. 請求項1~3または5のいずれか一項に記載の核酸分子、および所望により請求項4または5に記載の核酸分子を保有するナス科の植物を選択する方法であって、請求項1~3または5のいずれか一項に記載の核酸分子、および所望により請求項4または5に記載の核酸分子、またはその一部を、ナス科の植物において選択し、請求項1~3または5のいずれか一項に記載の核酸分子、および所望により、請求項4または5に記載の核酸分子。 A method for selecting a solanaceae plant carrying a nucleic acid molecule according to any one of claims 1 to 3 or 5, and optionally a nucleic acid molecule according to claim 4 or 5, comprising selecting a solanaceae plant carrying a nucleic acid molecule according to any one of claims 1 to 3 or 5, and optionally a nucleic acid molecule according to claim 4 or 5, or a part thereof, and selecting a solanaceae plant carrying a nucleic acid molecule according to any one of claims 1 to 3 or 5, and optionally a nucleic acid molecule according to claim 4 or 5. 植物が、根瘤線虫に対して抵抗性を示すかどうかを決定する工程をさらに含む、請求項14に記載の方法。 The method of claim 14, further comprising a step of determining whether the plant is resistant to root-knot nematodes. 植物が、ソラナム・リコペルシクム植物である、請求項14または15に記載の方法。 The method according to claim 14 or 15, wherein the plant is a Solanum lycopersicum plant. 根瘤線虫が、メロイドジン・エンテロロビイである、請求項14~16のいずれか一項に記載の方法。 The method according to any one of claims 14 to 16, wherein the root-knot nematode is Meloidogyne enterolobidi. 根瘤線虫に対する抵抗性を有する、ナス科の植物、特にソラム・リコペルシム植物を生産する方法であって、以下:
(a)請求項1~3および5のいずれか一項に記載の核酸分子および所望により請求項4または5に記載の核酸分子を含む植物を別の植物と交配すること;
(b)所望により、1回または複数回の自家受粉および/または交配を実施すること;
(c)1回または複数回の自家受粉および/または交配の後に、請求項1~3または5のいずれか一項に記載の該核酸分子および所望により請求項4または5に記載の核酸分子を含む植物を選択すること
を含む、方法。
1. A method for producing a Solanaceae plant, in particular a Solanum lycopersicum plant, resistant to root-knot nematodes, comprising the steps of:
(a) crossing a plant containing a nucleic acid molecule according to any one of claims 1 to 3 and 5 and optionally a nucleic acid molecule according to claim 4 or 5 with another plant;
(b) optionally performing one or more rounds of self-pollination and/or crossing;
(c) after one or more rounds of self-pollination and/or crossing, selecting a plant which comprises said nucleic acid molecule according to any one of claims 1 to 3 or 5 and optionally a nucleic acid molecule according to claim 4 or 5.
根瘤線虫に対して抵抗性であり、かつ請求項1~3または5のいずれか一項に記載の核酸分子および所望により請求項4または5に記載の核酸分子を含むナス科の植物の選択が、請求項14に記載の方法に従って核酸分子の存在を決定することをさらに含む、請求項18に記載の方法。 The method according to claim 18, wherein the selection of a solanaceous plant that is resistant to root-knot nematodes and contains a nucleic acid molecule according to any one of claims 1 to 3 or 5 and optionally a nucleic acid molecule according to claim 4 or 5 further comprises determining the presence of the nucleic acid molecule according to the method according to claim 14. 前記根瘤線虫が、メロイドジン・エンテロロビイである、請求項18~19のいずれか一項に記載の方法。 The method according to any one of claims 18 to 19, wherein the root-knot nematode is Meloidogyne enterolobidi. 根瘤線虫に対して抵抗性であるナス科の植物、特にソラナム・リコペルシム植物を生産するための、所望により請求項4または5に記載の核酸分子と一緒の、請求項1~3または5のいずれか一項に記載の核酸分子の使用。 Use of a nucleic acid molecule according to any one of claims 1 to 3 or 5, optionally together with a nucleic acid molecule according to claim 4 or 5, for producing a Solanaceae plant, in particular a Solanum lycopersicum plant, which is resistant to root-knot nematodes. 請求項1~3または5のいずれか一項に記載の核酸分子を、所望により請求項4または5に記載の核酸分子と共に、特に遺伝子移入により、ゲノム中に導入することにより、抵抗性である植物が生産される、請求項21に記載の使用。 The use according to claim 21, in which a resistant plant is produced by introducing into the genome, in particular by gene transfer, a nucleic acid molecule according to any one of claims 1 to 3 or 5, optionally together with a nucleic acid molecule according to claim 4 or 5. 根瘤線虫が、メロイドジン・エンテロロビイである、請求項21または22のいずれか一項に記載の使用。 The use according to any one of claims 21 or 22, wherein the root-knot nematode is Meloidogyne enterolobidi.
JP2022578622A 2020-06-18 2021-06-18 Root-knot nematode resistance-conferring gene Pending JP2023530491A (en)

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EPPCT/EP2020/067028 2020-06-18
EPPCT/EP2020/067028 2020-06-18
PCT/EP2021/066682 WO2021255272A1 (en) 2020-06-18 2021-06-18 Root-knot nematode resistance conferring gene

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US (1) US20230287062A1 (en)
EP (1) EP4168430A1 (en)
JP (1) JP2023530491A (en)
CN (1) CN115812076A (en)
BR (1) BR112022025676A2 (en)
IL (1) IL299078A (en)
MX (1) MX2022015428A (en)
WO (1) WO2021255272A1 (en)

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AU2001277459A1 (en) * 2000-06-09 2001-12-17 Institut Fur Pflanzengenetik Und Kulturpflanzen Forschung Hero resistance gene conferring resistance to nematodes
CN102268482B (en) * 2011-08-10 2013-01-23 中国农业大学 Meloidogyne enterolobii detection kit based on loop-mediated isothermal amplification and application thereof
WO2019108619A1 (en) * 2017-11-28 2019-06-06 Two Blades Foundation Methods and compositions for enhancing the disease resistance of plants

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