JPWO2021221789A5 - - Google Patents
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- JPWO2021221789A5 JPWO2021221789A5 JP2022553645A JP2022553645A JPWO2021221789A5 JP WO2021221789 A5 JPWO2021221789 A5 JP WO2021221789A5 JP 2022553645 A JP2022553645 A JP 2022553645A JP 2022553645 A JP2022553645 A JP 2022553645A JP WO2021221789 A5 JPWO2021221789 A5 JP WO2021221789A5
- Authority
- JP
- Japan
- Prior art keywords
- target molecule
- hapten
- probe
- initiator
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 27
- 239000003999 initiator Substances 0.000 claims 17
- 239000000523 sample Substances 0.000 claims 17
- 239000000178 monomer Substances 0.000 claims 10
- 239000000758 substrate Substances 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 210000001255 hallux Anatomy 0.000 claims 2
- 238000011534 incubation Methods 0.000 claims 2
- 230000004807 localization Effects 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 210000000453 second toe Anatomy 0.000 claims 2
Claims (25)
以下を準備すること：
第１分割イニシエーターを含む第１分割イニシエータープローブ；
第２分割イニシエーターを含む第２分割イニシエータープローブ；
以下を含む第１ヘアピン単量体：
第１トーホールドおよび第１ステム部を含む第１インプットドメイン、
第１ヘアピンループおよび前記第１ステム部に対する相補鎖を含む第１アウトプットドメイン、並びに
第１ハプテン分子；
以下を含む第２ヘアピン単量体：
第２トーホールドおよび第２ステム部を含む第２インプットドメイン、
第２ヘアピンループおよび前記第２ステム部に対する相補鎖を含む第２アウトプットドメイン、並びに
第２ハプテン分子；
標的分子；並びに
前記第１分割イニシエータープローブおよび前記第２分割イニシエータープローブを、前記標的分子と一緒にインキュベーションすること、を含む、
方法。 A method comprising:
Prepare the following:
a first split initiator probe including a first split initiator;
a second split initiator probe including a second split initiator;
A first hairpin monomer comprising:
a first input domain including a first toe hold and a first stem portion;
a first output domain comprising a first hairpin loop and a complementary strand to the first stem portion; and a first hapten molecule;
A second hairpin monomer comprising:
a second input domain including a second toe hold and a second stem portion;
a second output domain comprising a second hairpin loop and a complementary strand to the second stem portion; and a second hapten molecule;
a target molecule; and incubating the first split initiator probe and the second split initiator probe with the target molecule .
Method.
前記第２ヘアピン単量体を前記第１ヘアピン単量体に結合させること；
ＣＡＲＤを媒介する酵素である１または複数のレポーター体で標識された、抗ハプテン分子を準備すること；
１または複数のＣＡＲＤ基質を準備すること；
前記ＣＡＲＤを媒介する酵素によって前記ＣＡＲＤ基質から生成される、１または複数の沈着レポーターからのシグナルを測定すること、をさらに含む、請求項１に記載の方法。 binding the first hairpin monomer to both the first splitting initiator and the second splitting initiator;
bonding the second hairpin monomer to the first hairpin monomer;
providing an anti-hapten molecule labeled with one or more reporter bodies that are enzymes that mediate CARD;
providing one or more CARD substrates;
2. The method of claim 1 , further comprising measuring a signal from one or more deposited reporters generated from the CARD substrate by the CARD-mediating enzyme.
前記一次抗体と結合する二次抗体（１または複数のレポーター体で標識されている）を準備することをさらに含む、請求項４に記載の方法。5. The method of claim 4, further comprising providing a second antibody (labeled with one or more reporter bodies) that binds to the first antibody.
以下を準備すること：
少なくとも１つのイニシエーターを含む少なくとも１つのイニシエーター標識プローブ；
以下を含む第１ヘアピン単量体：
第１トーホールドおよび第１ステム部を含む第１インプットドメイン、
第１ヘアピンループおよび前記第１ステム部に対する相補鎖を含む第１アウトプットドメイン、並びに
第１ハプテン分子；
以下を含む第２ヘアピン単量体：
第２トーホールドおよび第２ステム部を含む第２インプットドメイン、
第２ヘアピンループおよび前記第２ステム部に対する相補鎖を含む第２アウトプットドメイン、並びに
第２ハプテン分子、
標的分子；並びに
前記少なくとも１つのイニシエーターを含む少なくとも１つのイニシエーター標識プローブを前記標的分子と一緒にインキュベーションすること、を含む、
方法。 A method comprising:
Prepare the following:
at least one initiator-labeled probe comprising at least one initiator;
A first hairpin monomer comprising:
a first input domain including a first toe hold and a first stem portion;
a first output domain comprising a first hairpin loop and a complementary strand to the first stem portion; and a first hapten molecule;
A second hairpin monomer comprising:
a second input domain including a second toe hold and a second stem portion;
a second output domain comprising a second hairpin loop and a complementary strand to the second stem portion; and a second hapten molecule;
a target molecule; and incubating at least one initiator-labeled probe comprising the at least one initiator with the target molecule .
Method.
前記第２ヘアピン単量体を前記第１ヘアピン単量体に結合させること；
ＣＡＲＤを媒介する酵素である１または複数のレポーター体で標識された、抗ハプテン分子を準備すること；
１または複数のＣＡＲＤ基質を準備すること；
前記ＣＡＲＤを媒介する酵素によって前記ＣＡＲＤ基質から生成される、１または複数の沈着レポーターからのシグナルを測定すること、をさらに含む、請求項１４に記載の方法。 binding the first hairpin monomer to the at least one initiator;
bonding the second hairpin monomer to the first hairpin monomer;
providing an anti-hapten molecule labeled with one or more reporter bodies that are enzymes that mediate CARD;
providing one or more CARD substrates;
15. The method of claim 14 , further comprising measuring a signal from one or more deposited reporters generated from the CARD substrate by the CARD-mediating enzyme.
前記一次抗体と結合する二次抗体（１または複数のレポーター体で標識されている）を準備することをさらに含む、請求項１８に記載の方法。 the anti-hapten antibody comprises a primary antibody that binds to the hapten;
19. The method of claim 18 , further comprising providing a second antibody (labeled with one or more reporter bodies) that binds to the first antibody.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062986436P | 2020-03-06 | 2020-03-06 | |
| US62/986,436 | 2020-03-06 | ||
| PCT/US2021/020919 WO2021221789A2 (en) | 2020-03-06 | 2021-03-04 | Analysis of target molecules within a sample via hybridization chain reaction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023517054A JP2023517054A (en) | 2023-04-21 |
| JPWO2021221789A5 true JPWO2021221789A5 (en) | 2024-03-12 |
Family
ID=78332110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022553645A Pending JP2023517054A (en) | 2020-03-06 | 2021-03-04 | Analysis of target molecules within a sample via hybridization chain reaction |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20220282300A1 (en) |
| EP (1) | EP4114969A4 (en) |
| JP (1) | JP2023517054A (en) |
| KR (1) | KR20220151183A (en) |
| CN (1) | CN115516105A (en) |
| AU (1) | AU2021265719A1 (en) |
| CA (1) | CA3173975A1 (en) |
| IL (1) | IL295981A (en) |
| WO (1) | WO2021221789A2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4284925A1 (en) | 2021-01-26 | 2023-12-06 | California Institute of Technology | Allosteric conditional guide rnas for cell-selective regulation of crispr/cas |
| WO2023159240A1 (en) | 2022-02-21 | 2023-08-24 | Kanvas Biosciences, Inc. | Highly multiplexed detection of gene expression with hybridization chain reaction |
| CN115961011A (en) * | 2022-12-19 | 2023-04-14 | 中山大学 | Method for detecting formaldehyde based on gold nanoparticle and hybrid chain reaction amplification |
| CN116206684B (en) * | 2022-12-26 | 2024-01-30 | 纳昂达(南京)生物科技有限公司 | Method and device for evaluating capture safety of genome repeated region probe |
| US20240336956A1 (en) * | 2023-04-04 | 2024-10-10 | California Institute Of Technology | Ultrasensitive molecular detection via hybridization chain reaction |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005098049A2 (en) | 2004-03-25 | 2005-10-20 | California Institute Of Technology | Hybridization chain reaction |
| EP1799825B1 (en) * | 2004-10-05 | 2011-06-29 | The California Institute of Technology | Aptamer regulated nucleic acids and uses thereof |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| US8793705B2 (en) | 2007-08-31 | 2014-07-29 | Red Hat, Inc. | Rich internet bus |
| GB201401885D0 (en) * | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| AU2017291727B2 (en) | 2016-07-05 | 2021-07-08 | California Institute Of Technology | Fractional initiator hybridization chain reaction |
| WO2018217905A1 (en) * | 2017-05-23 | 2018-11-29 | Rutgers, The State University Of New Jersey | Target mediated in situ signal amplification with dual interacting hairpin probes |
-
2021
- 2021-03-04 KR KR1020227034305A patent/KR20220151183A/en unknown
- 2021-03-04 US US17/637,253 patent/US20220282300A1/en active Pending
- 2021-03-04 CA CA3173975A patent/CA3173975A1/en active Pending
- 2021-03-04 JP JP2022553645A patent/JP2023517054A/en active Pending
- 2021-03-04 AU AU2021265719A patent/AU2021265719A1/en active Pending
- 2021-03-04 CN CN202180033401.5A patent/CN115516105A/en active Pending
- 2021-03-04 EP EP21796673.8A patent/EP4114969A4/en active Pending
- 2021-03-04 IL IL295981A patent/IL295981A/en unknown
- 2021-03-04 WO PCT/US2021/020919 patent/WO2021221789A2/en active Application Filing
-
2022
- 2022-02-28 US US17/682,336 patent/US20230193362A1/en active Pending
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