JPWO2021089841A5 - - Google Patents
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- JPWO2021089841A5 JPWO2021089841A5 JP2022525257A JP2022525257A JPWO2021089841A5 JP WO2021089841 A5 JPWO2021089841 A5 JP WO2021089841A5 JP 2022525257 A JP2022525257 A JP 2022525257A JP 2022525257 A JP2022525257 A JP 2022525257A JP WO2021089841 A5 JPWO2021089841 A5 JP WO2021089841A5
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- container
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- monochromatic light
- light source
- photodetector
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Claims (18)
－ 溶液中の前記粒子のサンプルを含む容器を提供するステップと、
－ 少なくとも１つの単色光源および少なくとも１つの光検出器を提供するステップと、
－ 前記単色光源からの光を前記サンプルを含む前記容器へ伝送するステップと、
－ 前記容器から放出された光を前記光検出器によって検出するステップと、
－ 前記サンプルに含まれる溶液中の前記粒子および／または前記容器の材料の蛍光を測定するステップと、
－ 前記測定された蛍光に基づいて、前記容器の位置を判定するステップと、
－ 動的光散乱（ＤＬＳ）測定に基づいて、前記サンプルに含まれる溶液中の前記粒子の特徴を判定するステップとを含む方法。 A method for measuring characteristics of particles in a solution, the method comprising:
- providing a container containing a sample of said particles in solution ;
- providing at least one monochromatic light source and at least one photodetector;
- transmitting light from the monochromatic light source to the container containing the sample;
- detecting light emitted from the container by the photodetector;
- measuring the fluorescence of the particles in the solution contained in the sample and/or the material of the container;
- determining the position of the container based on the measured fluorescence;
- determining characteristics of the particles in a solution contained in the sample based on dynamic light scattering (DLS) measurements.
－ 溶液中の前記粒子のサンプルを含む容器を提供するステップと、
－ 少なくとも１つの単色光源および少なくとも１つの光検出器を提供するステップと、
－ 前記単色光源からの光を前記サンプルを含む前記容器へ伝送するステップと、
－ 前記容器から放出された光を前記光検出器によって検出するステップと、
－ 前記サンプルに含まれる溶液中の前記粒子および／または前記容器の材料の蛍光を測定するステップと、
－ 前記サンプルを含む前記容器の後方反射を測定し、前記測定された後方反射に基づいて、前記容器の位置を判定するステップと、
－ 動的光散乱（ＤＬＳ）測定に基づいて、前記サンプルに含まれる溶液中の前記粒子の特徴を判定するステップと
を含む方法。 A method for measuring characteristics of particles in a solution, the method comprising:
- providing a container containing a sample of said particles in solution;
- providing at least one monochromatic light source and at least one photodetector;
- transmitting light from the monochromatic light source to the container containing the sample;
- detecting light emitted from the container by the photodetector;
- measuring the fluorescence of the particles in the solution contained in the sample and/or the material of the container;
- measuring the back reflection of the container containing the sample and determining the position of the container based on the measured back reflection ;
- determining characteristics of the particles in a solution contained in the sample based on dynamic light scattering (DLS) measurements;
method including.
を含む、請求項１または２に記載の方法。 - positioning the container based on the measured fluorescence and/or back reflection and the determined container position.
をさらに含む、請求項１から３のいずれか一項に記載の方法。 - performing nano differential scanning fluorimetry (nano DSF) measurements;
4. The method according to any one of claims 1 to 3, further comprising:
各容器に対するＤＬＳ測定に続いて、各容器に対する蛍光測定が行われ、または
複数の容器のうちの１つの容器に対して蛍光測定およびＤＬＳ測定が実行されるのに続いて、前記複数の容器のうちの別の容器に対して蛍光測定およびＤＬＳ測定が実行される、
請求項１から４のいずれか一項に記載の方法。 A fluorescence measurement for each container is followed by a DLS measurement for each container, or a DLS measurement for each container is followed by a fluorescence measurement for each container, or for one of the containers. After the fluorescence measurement and DLS measurement are performed, the fluorescence measurement and DLS measurement are performed on another container of the plurality of containers.
A method according to any one of claims 1 to 4.
－ 前記光検出器から取得されたアナログ出力信号を取得するステップと、
－ 前記取得されたアナログ出力信号を処理するステップとを含み、前記取得されたアナログ出力信号を処理する前記ステップが、前記取得されたアナログ出力信号を、デジタル化された出力信号にデジタル化するステップを含み、前記デジタル化された出力信号が、
ｉ）前記デジタル化された出力信号を、デジタル化された単一光子パルス信号として処理するステップ、
および／または
ｉｉ）前記デジタル化された出力信号を、アナログ信号の離散値として処理するステップ
によってさらに処理される、
請求項１から９のいずれか一項に記載の方法。 The DLS measurement is
- obtaining an analog output signal obtained from the photodetector;
- processing the obtained analog output signal, the step of processing the obtained analog output signal digitizing the obtained analog output signal into a digitized output signal. , the digitized output signal comprising:
i) processing the digitized output signal as a digitized single photon pulse signal;
and/or i i) further processed by processing said digitized output signal as discrete values of an analog signal;
A method according to any one of claims 1 to 9 .
をさらに含む、請求項１から１０のいずれか一項に記載の方法。 - a method according to any one of claims 1 to 10 , further comprising: - temperature conditioning the container over time, at least at a first temperature at a first point in time and at a second temperature at a second point in time. .
－ 溶液中の前記粒子のサンプルを含む少なくとも１つの容器を収容する手段と、
－ 単色光源および光検出器と、
－ ＤＳＬ測定を実行する手段と、
－ 前記サンプルに含まれる溶液中の前記粒子および／または前記容器の材料の、蛍光を測定する手段と、
－ 制御手段とを備え、前記制御手段が、
少なくとも１つの容器を収容する前記手段を制御し、
前記単色光源からの光を前記少なくとも１つの容器へ伝送するように前記単色光源を制御し、
前記少なくとも１つの容器からの信号を検出するように前記光検出器を制御し、
前記蛍光を測定する前記手段を制御し、
ＤＳＬ測定を実行する前記手段を制御し、
前記測定された蛍光に基づいて、前記容器の位置を判定する手段を制御するように適合される、デバイス。 A device for detecting characteristics of particles in a solution, the device comprising:
- means for containing at least one container containing a sample of said particles in solution;
- a monochromatic light source and a photodetector;
- means for performing DSL measurements;
- means for measuring the fluorescence of the particles in the solution contained in the sample and/or the material of the container;
- control means, the control means comprising:
controlling said means for containing at least one container;
controlling the monochromatic light source to transmit light from the monochromatic light source to the at least one container;
controlling the photodetector to detect a signal from the at least one container;
controlling the means for measuring the fluorescence;
controlling said means for performing DSL measurements ;
A device adapted to control means for determining the position of the container based on the measured fluorescence .
請求項１４に記載のデバイス。 - further comprising positioning means for positioning said means containing said sample of said particles in solution, said control means further adapted to control said positioning means containing said sample;
15. A device according to claim 14 .
請求項１４または１５に記載のデバイス。 - further comprising means for performing nano-DSF measurements and/or means for measuring back-reflections, further comprising: said control means controlling said means for performing nano-DSF measurements and/or said means for measuring back-reflections; adapted,
16. A device according to claim 14 or 15 .
－ 前記単一モードファイバを介して前記単色光源からの単色光を送達する手段とをさらに備え、前記制御手段が、前記単一モードファイバを介して前記単色光源からの単色光を送達する前記手段を制御するようにさらに適合される、
請求項１４から１６のいずれか一項に記載のデバイス。 - single mode fiber;
- means for delivering monochromatic light from the monochromatic light source via the single mode fiber, the control means further comprising: means for delivering monochromatic light from the monochromatic light source via the single mode fiber; further adapted to control the
17. A device according to any one of claims 14 to 16 .
－ 前記蛍光測定のためのＬＥＤ光源と、
－ 前記後方反射測定のためのさらなるＬＥＤと、
－ 前記動的光散乱（ＤＬＳ）測定のための少なくとも１つの光検出器と、
－ 前記蛍光測定のための少なくとも１つの追加の光検出器と、
－ 前記後方反射測定のための１つの追加の光検出器と
を備える、請求項１４から１７のいずれか一項に記載のデバイス。 - the at least one monochromatic light source for the DLS measurement ;
- an LED light source for said fluorescence measurement ;
- a further LED for said back reflection measurement ;
- at least one photodetector for said dynamic light scattering (DLS) measurements;
- at least one additional photodetector for said fluorescence measurement;
- one additional photodetector for said back reflection measurement;
18. A device according to any one of claims 14 to 17 , comprising:
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19208187 | 2019-11-08 | ||
| EP19208187.5 | 2019-11-08 | ||
| EP20197910.1 | 2020-09-23 | ||
| EP20197910 | 2020-09-23 | ||
| PCT/EP2020/081378 WO2021089841A1 (en) | 2019-11-08 | 2020-11-06 | Characterization of particles in solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023501942A JP2023501942A (en) | 2023-01-20 |
| JPWO2021089841A5 true JPWO2021089841A5 (en) | 2023-11-06 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022525259A Active JP7461078B2 (en) | 2019-11-08 | 2020-11-06 | Characterization of particles in solution |
| JP2022525257A Pending JP2023501942A (en) | 2019-11-08 | 2020-11-06 | Characterization of particles in solution |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022525259A Active JP7461078B2 (en) | 2019-11-08 | 2020-11-06 | Characterization of particles in solution |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20220404284A1 (en) |
| EP (2) | EP4055366A1 (en) |
| JP (2) | JP7461078B2 (en) |
| KR (2) | KR20220093215A (en) |
| CN (2) | CN114930151A (en) |
| AU (2) | AU2020378640A1 (en) |
| CA (1) | CA3153480A1 (en) |
| WO (2) | WO2021089841A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4328569A1 (en) * | 2022-08-23 | 2024-02-28 | NanoTemper Technologies GmbH | Measuring high protein concentrations |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5328822A (en) * | 1990-04-23 | 1994-07-12 | Solid State Farms, Inc. | Apparatus and method for sedimentation based blood analysis |
| US5973779A (en) * | 1996-03-29 | 1999-10-26 | Ansari; Rafat R. | Fiber-optic imaging probe |
| FR2856792B1 (en) * | 2003-06-27 | 2006-09-15 | Commissariat Energie Atomique | METHOD AND DEVICE FOR ASSAYING A BIOLOGICAL OR CHEMICAL SAMPLE |
| CA2677123A1 (en) * | 2007-02-02 | 2008-08-07 | Canadian Blood Services | Method of detecting bacterial contamination using dynamic light scattering |
| DE102014018535A1 (en) | 2014-12-12 | 2016-06-16 | Nanotemper Technologies Gmbh | System and method for a seal-free tempering of capillaries |
| EP4242304A3 (en) * | 2015-10-01 | 2024-02-07 | Revolution Medicines, Inc. | Methods and reagents for analyzing protein-protein interfaces |
| EP3159674A1 (en) * | 2015-10-19 | 2017-04-26 | European Molecular Biology Laboratory | Measurement cell for saxs measurements and for dls measurements |
| US10473591B2 (en) * | 2017-05-01 | 2019-11-12 | Wyatt Technology Corporation | High throughput method and apparatus for measuring multiple optical properties of a liquid sample |
| WO2019166650A1 (en) * | 2018-03-02 | 2019-09-06 | Cdr-Life Ag | Trispecific antigen binding proteins |
| WO2019201894A1 (en) * | 2018-04-16 | 2019-10-24 | Merck Patent Gmbh | Additives for protein formulations to improve thermal stability |
-
2020
- 2020-11-06 KR KR1020227019318A patent/KR20220093215A/en unknown
- 2020-11-06 JP JP2022525259A patent/JP7461078B2/en active Active
- 2020-11-06 WO PCT/EP2020/081378 patent/WO2021089841A1/en unknown
- 2020-11-06 US US17/775,400 patent/US20220404284A1/en active Pending
- 2020-11-06 CA CA3153480A patent/CA3153480A1/en active Pending
- 2020-11-06 EP EP20800668.4A patent/EP4055366A1/en active Pending
- 2020-11-06 AU AU2020378640A patent/AU2020378640A1/en active Pending
- 2020-11-06 US US17/775,466 patent/US20220373557A1/en active Pending
- 2020-11-06 EP EP20800216.2A patent/EP4055365A1/en active Pending
- 2020-11-06 CN CN202080092388.6A patent/CN114930151A/en active Pending
- 2020-11-06 KR KR1020227019316A patent/KR20220092620A/en unknown
- 2020-11-06 CN CN202080092390.3A patent/CN114930152A/en active Pending
- 2020-11-06 JP JP2022525257A patent/JP2023501942A/en active Pending
- 2020-11-06 WO PCT/EP2020/081370 patent/WO2021089834A1/en unknown
- 2020-11-06 AU AU2020379227A patent/AU2020379227A1/en active Pending
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