JPWO2020250998A5 - - Google Patents
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- JPWO2020250998A5 JPWO2020250998A5 JP2021526143A JP2021526143A JPWO2020250998A5 JP WO2020250998 A5 JPWO2020250998 A5 JP WO2020250998A5 JP 2021526143 A JP2021526143 A JP 2021526143A JP 2021526143 A JP2021526143 A JP 2021526143A JP WO2020250998 A5 JPWO2020250998 A5 JP WO2020250998A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- enzyme
- cells
- staining
- fluorescent compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000790 Enzymes Proteins 0.000 claims 7
- 102000004190 Enzymes Human genes 0.000 claims 7
- 239000007850 fluorescent dye Substances 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 238000010186 staining Methods 0.000 claims 4
- -1 PDL-2 Proteins 0.000 claims 3
- 102000001301 EGF receptor Human genes 0.000 claims 2
- 108060006698 EGF receptor Proteins 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- NAWXUBYGYWOOIX-SFHVURJKSA-N (2s)-2-[[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]amino]-4-methylidenepentanedioic acid Chemical compound C1=CC2=NC(N)=NC(N)=C2C=C1CCC1=CC=C(C(=O)N[C@@H](CC(=C)C(O)=O)C(O)=O)C=C1 NAWXUBYGYWOOIX-SFHVURJKSA-N 0.000 claims 1
- 102100023990 60S ribosomal protein L17 Human genes 0.000 claims 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 101710149863 C-C chemokine receptor type 4 Proteins 0.000 claims 1
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 claims 1
- 102100032976 CCR4-NOT transcription complex subunit 6 Human genes 0.000 claims 1
- 101150013553 CD40 gene Proteins 0.000 claims 1
- 102100032912 CD44 antigen Human genes 0.000 claims 1
- 101100463133 Caenorhabditis elegans pdl-1 gene Proteins 0.000 claims 1
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 claims 1
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 claims 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 claims 1
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims 1
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 claims 1
- 101000934338 Homo sapiens Myeloid cell surface antigen CD33 Proteins 0.000 claims 1
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims 1
- 101000884271 Homo sapiens Signal transducer CD24 Proteins 0.000 claims 1
- 101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 claims 1
- 101000621309 Homo sapiens Wilms tumor protein Proteins 0.000 claims 1
- 102100025304 Integrin beta-1 Human genes 0.000 claims 1
- 101100058550 Mus musculus Bmi1 gene Proteins 0.000 claims 1
- 102100025243 Myeloid cell surface antigen CD33 Human genes 0.000 claims 1
- 101710089372 Programmed cell death protein 1 Proteins 0.000 claims 1
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims 1
- 102100038081 Signal transducer CD24 Human genes 0.000 claims 1
- 102100027222 T-lymphocyte activation antigen CD80 Human genes 0.000 claims 1
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 claims 1
- 102100022748 Wilms tumor protein Human genes 0.000 claims 1
- 102000005936 beta-Galactosidase Human genes 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000000684 flow cytometry Methods 0.000 claims 1
- 238000002795 fluorescence method Methods 0.000 claims 1
- 238000000799 fluorescence microscopy Methods 0.000 claims 1
- 238000011896 sensitive detection Methods 0.000 claims 1
- 238000007447 staining method Methods 0.000 claims 1
Claims (7)
前記酵素標識抗体が結合した前記標的細胞に、下記の式（１）から（１０）のいずれかで表される細胞滞留性蛍光化合物を接触させる工程を含む細胞の染色方法。
A method for staining cells, comprising the step of contacting the target cells to which the enzyme-labeled antibody is bound with a cell-retaining fluorescent compound represented by any one of the following formulas (1) to (10) .
蛍光法を用いて染色された前記標的細胞を検出する工程を含む細胞の高感度検出方法。 A step of staining target cells using the cell staining method according to any one of claims 2 to 5 ;
A highly sensitive detection method for cells, comprising the step of detecting the stained target cells using a fluorescence method.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019111159A JP2023012556A (en) | 2019-06-14 | 2019-06-14 | Cell retaining fluorescent compound as well as dyeing method of cell therewith and high sensitivity detection method |
| PCT/JP2020/023120 WO2020250998A1 (en) | 2019-06-14 | 2020-06-12 | Intracellular retentive fluorescent compound, and cell staining method and high-sensitivity cell detection method each using same |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2020250998A1 JPWO2020250998A1 (en) | 2020-12-17 |
| JPWO2020250998A5 true JPWO2020250998A5 (en) | 2023-08-15 |
| JP7385850B2 JP7385850B2 (en) | 2023-11-24 |
Family
ID=73781052
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019111159A Pending JP2023012556A (en) | 2019-06-14 | 2019-06-14 | Cell retaining fluorescent compound as well as dyeing method of cell therewith and high sensitivity detection method |
| JP2021526143A Active JP7385850B2 (en) | 2019-06-14 | 2020-06-12 | Cell-retentive fluorescent compound, cell staining method using the same, and high-sensitivity detection method |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019111159A Pending JP2023012556A (en) | 2019-06-14 | 2019-06-14 | Cell retaining fluorescent compound as well as dyeing method of cell therewith and high sensitivity detection method |
Country Status (2)
| Country | Link |
|---|---|
| JP (2) | JP2023012556A (en) |
| WO (1) | WO2020250998A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116621897A (en) * | 2023-05-08 | 2023-08-22 | 中国药科大学 | Scopolamine derivatives, preparation method and medical application thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015124703A1 (en) * | 2014-02-24 | 2015-08-27 | Ventana Medical Systems, Inc. | Quinone methide analog signal amplification |
| CN106459125B (en) * | 2014-05-14 | 2019-05-07 | 国立大学法人东京大学 | The Intracellular retention fluorescent chemicals of enzyme spcificity |
| JP6849983B2 (en) * | 2016-06-30 | 2021-03-31 | 国立大学法人 東京大学 | Enzyme-specific intracellular retention red fluorescent probe. |
| WO2018079859A1 (en) * | 2016-10-26 | 2018-05-03 | 国立大学法人九州大学 | Cell labeling method |
| JP2020536874A (en) * | 2017-10-04 | 2020-12-17 | バッテル メモリアル インスティチュート | Probes for selectively characterization of enzymes involved in foreign body metabolism, as well as methods for their production and use. |
-
2019
- 2019-06-14 JP JP2019111159A patent/JP2023012556A/en active Pending
-
2020
- 2020-06-12 WO PCT/JP2020/023120 patent/WO2020250998A1/en active Application Filing
- 2020-06-12 JP JP2021526143A patent/JP7385850B2/en active Active
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