JPWO2020040257A1 - 改変ホスホリラーゼを利用したラクト−Ｎ−ビオースＩまたはガラクト−Ｎ−ビオースのβグリコシドの酵素合成法 - Google Patents

改変ホスホリラーゼを利用したラクト−Ｎ−ビオースＩまたはガラクト−Ｎ−ビオースのβグリコシドの酵素合成法 Download PDF

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JPWO2020040257A1
JPWO2020040257A1 JP2020538465A JP2020538465A JPWO2020040257A1 JP WO2020040257 A1 JPWO2020040257 A1 JP WO2020040257A1 JP 2020538465 A JP2020538465 A JP 2020538465A JP 2020538465 A JP2020538465 A JP 2020538465A JP WO2020040257 A1 JPWO2020040257 A1 JP WO2020040257A1
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glnbp
seq
amino acid
modified
acid sequence
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Japanese (ja)
Inventor
高嶺 片山
高嶺 片山
加藤　紀彦
紀彦 加藤
北岡　本光
本光 北岡
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Kyoto University
National Agriculture and Food Research Organization
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Kyoto University
National Agriculture and Food Research Organization
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020538465A 2018-08-24 2019-08-22 改変ホスホリラーゼを利用したラクト−Ｎ−ビオースＩまたはガラクト−Ｎ−ビオースのβグリコシドの酵素合成法 Pending JPWO2020040257A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2018157840 2018-08-24
JP2018157840 2018-08-24
PCT/JP2019/032866 WO2020040257A1 (fr) 2018-08-24 2019-08-22 PROCÉDÉ DE SYNTHÈSE ENZYMATIQUE POUR DES β-GLYCOSIDES DE LACTO-N-BIOSE I OU DE GALACTO-N-BIOSE À L'AIDE DE PHOSPHORYLASE MODIFIÉE

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JPWO2020040257A1 true JPWO2020040257A1 (ja) 2021-08-10

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JP2020538465A Pending JPWO2020040257A1 (ja) 2018-08-24 2019-08-22 改変ホスホリラーゼを利用したラクト−Ｎ−ビオースＩまたはガラクト−Ｎ−ビオースのβグリコシドの酵素合成法

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JP (1) JPWO2020040257A1 (fr)
WO (1) WO2020040257A1 (fr)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023151476A1 (fr) * 2022-02-14 2023-08-17 山东恒鲁生物科技有限公司 Glycoside hydrolase, son procédé de préparation et son application

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11253191A (ja) * 1998-03-13 1999-09-21 Meiji Milk Prod Co Ltd ラクト−ｎ−テトラオースの合成法
JP2014168404A (ja) * 2013-03-01 2014-09-18 National Agriculture & Food Research Organization 耐熱性１，３−βガラクトシル−Ｎ−アセチルヘキソサミンホスホリラーゼ
JP6222647B2 (ja) * 2013-03-26 2017-11-01 国立研究開発法人農業・食品産業技術総合研究機構 １，３−βガラクトシル−Ｎ−アセチルヘキソサミンホスホリラーゼの製造方法

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