JPWO2019195633A5 - - Google Patents
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- JPWO2019195633A5 JPWO2019195633A5 JP2020554139A JP2020554139A JPWO2019195633A5 JP WO2019195633 A5 JPWO2019195633 A5 JP WO2019195633A5 JP 2020554139 A JP2020554139 A JP 2020554139A JP 2020554139 A JP2020554139 A JP 2020554139A JP WO2019195633 A5 JPWO2019195633 A5 JP WO2019195633A5
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- 238000000034 method Methods 0.000 claims description 86
- 239000007787 solid Substances 0.000 claims description 47
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000039446 nucleic acids Human genes 0.000 claims description 19
- 108020004707 nucleic acids Proteins 0.000 claims description 19
- 150000007523 nucleic acids Chemical class 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000005096 rolling process Methods 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 3
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 2
- 230000009881 electrostatic interaction Effects 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 239000010936 titanium Substances 0.000 claims description 2
- 229910052719 titanium Inorganic materials 0.000 claims description 2
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims description 2
- 102000053602 DNA Human genes 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 108010043958 Peptoids Proteins 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 229910052804 chromium Inorganic materials 0.000 claims 1
- 239000011651 chromium Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 239000000203 mixture Substances 0.000 description 15
- 150000005829 chemical entities Chemical class 0.000 description 14
- 125000000524 functional group Chemical group 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 239000000412 dendrimer Substances 0.000 description 3
- 229920000736 dendritic polymer Polymers 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 150000001345 alkine derivatives Chemical class 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000001268 conjugating effect Effects 0.000 description 2
- 150000002118 epoxides Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- -1 cycloalkyne Chemical class 0.000 description 1
- WJTCGQSWYFHTAC-UHFFFAOYSA-N cyclooctane Chemical compound C1CCCCCCC1 WJTCGQSWYFHTAC-UHFFFAOYSA-N 0.000 description 1
- 239000004914 cyclooctane Substances 0.000 description 1
- ZPWOOKQUDFIEIX-UHFFFAOYSA-N cyclooctyne Chemical compound C1CCCC#CCC1 ZPWOOKQUDFIEIX-UHFFFAOYSA-N 0.000 description 1
- AUZONCFQVSMFAP-UHFFFAOYSA-N disulfiram Chemical compound CCN(CC)C(=S)SSC(=S)N(CC)CC AUZONCFQVSMFAP-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- XOLBLPGZBRYERU-UHFFFAOYSA-N tin dioxide Chemical group O=[Sn]=O XOLBLPGZBRYERU-UHFFFAOYSA-N 0.000 description 1
- 229910001887 tin oxide Inorganic materials 0.000 description 1
Description
本発明の好ましい態様を本明細書において表示および記載したが、そのような態様は単に例として提供されることが当業者には明らかであろう。多数の変化、変更および置換が、本発明から逸脱することなく、当業者に今は想到されるだろう。本明細書に記載されている本発明の態様の様々な代替物が、本発明の実施において用いられ得ることが理解されるべきである。以下の特許請求の範囲は本発明の範囲を定義し、この特許請求の範囲の範囲内の方法および構造体ならびにそれらの等価物はそれによって保護されることが、意図される。
一態様において、本発明は以下を提供する。
（項目１）
タンパク質に共有結合している構造化核酸粒子（structured nucleic acid particle）(SNAP)を含む、組成物。
（項目２）
前記SNAPが固体支持体に結合している、項目1記載の組成物。
（項目３）
前記SNAPが固体支持体に共有結合している、項目2記載の組成物。
（項目４）
前記SNAPが固体支持体に非共有結合している、項目2記載の組成物。
（項目５）
生体分子に共有結合している核酸SNAPを含む、組成物。
（項目６）
前記SNAPが固体支持体に結合している、項目5記載の組成物。
（項目７）
前記SNAPが固体支持体に共有結合している、項目6記載の組成物。
（項目８）
前記SNAPが固体支持体に非共有結合している、項目6記載の組成物。
（項目９）
複数の構造化核酸粒子(SNAP)に結合している固体支持体を含む組成物であって、該複数のSNAPの各々が生体分子に結合している、組成物。
（項目１０）
前記複数のSNAPがアレイ状に配置されている、項目9記載の組成物。
（項目１１）
単一のタンパク質を固体支持体上の結合部位に結合させる方法であって、
該結合部位が該タンパク質より大きく；
該方法が、
該タンパク質を構造化核酸粒子(SNAP)に共有結合させる工程であって、該SNAPの直径が少なくとも該結合部位の直径と同じ大きさである、工程；および
該核酸SNAPを該結合部位に結合させる工程
を含む、方法。
（項目１２）
各結合部位が単一のタンパク質に結合するように、複数の前記タンパク質の各々が前記固体支持体上の結合部位に結合する、項目11記載の方法。
（項目１３）
固体支持体に結合している複数の生体分子を含む生体分子アレイであって、該複数の生体分子の各生体分子がリンカーに共有結合しており、該リンカーが該固体支持体に結合しており、かつ各リンカーが該複数の生体分子のうちの一つの生体分子のみに結合しており、各リンカーが少なくとも50nmの直径を有する、生体分子アレイ。
（項目１４）
複数のタンパク質から空間的に分離されているタンパク質のアレイを製造する方法であって、該複数のタンパク質の各タンパク質を、構造化核酸粒子(SNAP)を構成する核酸分子の末端に共有結合させる工程、該SNAPを固体支持体に結合させる工程を含み、それによって空間的に分離されているタンパク質のアレイを製造する、方法。
（項目１５）
タンパク質、構造化核酸粒子(SNAP)、および固体支持体を含む組成物であって、該タンパク質が該SNAPに共有結合しており、かつ該タンパク質が該固体支持体に接触していない、組成物。
（項目１６）
空間的に分離されている生物学的または化学的実体のアレイを製造する方法であって、
結合部位を有する固体支持体を得る工程、
複数の生物学的または化学的実体を含むサンプルを得る工程、
各々が官能基を有するシードを得る工程、
該複数の生物学的または化学的実体の各生物学的または化学的実体を、該官能基を介して単一のシードに共有結合させる工程、
結合した各シードを所望のサイズの構造化核酸粒子(SNAP)へと成長させる工程、
該SNAPを該アレイの結合部位に結合させる工程
を含み、
それによって、空間的に分離されている生物学的または化学的実体のアレイを製造する、方法。
（項目１７）
前記固体支持体がガラス支持体である、項目16記載の方法。
（項目１８）
前記固体支持体がシリカ支持体である、項目16記載の方法。
（項目１９）
前記固体支持体がプラスチック支持体である、項目16記載の方法。
（項目２０）
前記固体支持体がシリコン支持体である、項目16記載の方法。
（項目２１）
前記固体支持体が金支持体である、項目16記載の方法。
（項目２２）
前記固体支持体が金属支持体である、項目16記載の方法。
（項目２３）
前記固体支持体がクロム支持体である、項目16記載の方法。
（項目２４）
前記固体支持体がチタン支持体である、項目16記載の方法。
（項目２５）
前記固体支持体が酸化チタン支持体である、項目16記載の方法。
（項目２６）
前記固体支持体がスズ支持体である、項目16記載の方法。
（項目２７）
前記固体支持体が酸化スズ支持体である、項目16記載の方法。
（項目２８）
前記固体支持体が光学的に不透明である、項目16記載の方法。
（項目２９）
前記固体支持体が光学的に透明である、項目16記載の方法。
（項目３０）
前記固体支持体が、正電荷を有するように修飾されている、項目16記載の方法。
（項目３１）
前記固体支持体が、負電荷を有するように修飾されている、項目16記載の方法。
（項目３２）
前記固体支持体が、前記SNAPに結合し得る官能基を有するように修飾されている、項目16記載の方法。
（項目３３）
前記固体支持体が、周囲の表面とは異なるように修飾されている結合部位を含む、項目16記載の方法。
（項目３４）
前記固体支持体が結合部位のアレイを含む、項目27記載の方法。
（項目３５）
各結合部位が、他の結合部位の各々から少なくとも70nm離れている、項目34記載の方
法。
（項目３６）
各結合部位が、他の結合部位の各々から少なくとも25nm離れている、項目34記載の方法。
（項目３７）
任意の2つの結合部位の端部間の距離が、使用される前記SNAPの半径よりも大きい、項目34記載の方法。
（項目３８）
任意の2つの結合部位の端部間の距離が、使用される前記SNAPの直径よりも大きい、項目34記載の方法。
（項目３９）
前記分子がタンパク質である、項目16記載の方法。
（項目４０）
前記シードがオリゴヌクレオチドである、項目16記載の方法。
（項目４１）
前記オリゴヌクレオチドの3'末端が官能基で修飾されている、項目40記載の方法。
（項目４２）
前記オリゴヌクレオチドの5'末端が官能基で修飾されている、項目40記載の方法。
（項目４３）
前記官能基が、アミン、チオール、カルボン酸、三重結合、二重結合、エポキシド、アルキン、アルケン、シクロアルキン、アジド、シクロオクチン、シクロアルキン、ノルボルネン、テトラジン、シクロオクタン、エポキシド、およびヒドロキシルからなる群より選択される、項目41または42のいずれか一項記載の方法。
（項目４４）
前記オリゴヌクレオチドが、光切断可能な結合を含むように修飾されている、項目40記載の方法。
（項目４５）
前記SNAPがローリングサークル増幅によって形成される、項目16記載の方法。
（項目４６）
前記SNAPがデンドリマーである、項目16記載の方法。
（項目４７）
前記デンドリマーが正に帯電している、項目46記載の方法。
（項目４８）
前記アレイ上の結合部位が負に帯電している、項目47記載の方法。
（項目４９）
前記デンドリマーが負に帯電している、項目46記載の方法。
（項目５０）
前記アレイ上の結合部位が正に帯電している、項目49記載の方法。
（項目５１）
前記SNAPがおよそ100nmの直径を有する、項目50記載の方法。
（項目５２）
前記SNAPがおよそ300nmの直径を有する、項目45記載の方法。
（項目５３）
前記SNAPが約10nm～500μmの直径を有する、項目45記載の方法。
（項目５４）
前記SNAPが約10nm～50μmの直径を有する、項目45記載の方法。
（項目５５）
前記SNAPが約10nm～5μmの直径を有する、項目45記載の方法。
（項目５６）
前記SNAPが約100nm～500nmの直径を有する、項目45記載の方法。
（項目５７）
前記SNAPが、静電相互作用を通じて前記固体支持体に付着する、項目45記載の方法。
（項目５８）
分子の空間的分離を達成する方法であって、
複数の分子を得る工程、
各々が官能基を有するシードを得る工程、
該複数の分子の各々を、該官能基を介して単一のシードに共有結合させる工程、
結合した各シードを、所望のサイズの構造化核酸粒子(SNAP)へと成長させる工程、
該SNAPを固体支持体に結合させる工程
を含み、
それによって単一分子の空間的分離を達成する、方法。
（項目５９）
単一分子のアレイであって、各単一分子が所望のサイズの構造化核酸粒子(SNAP)に結合しており、該SNAPが該結合部位を介してアレイに結合している、単一分子のアレイ。
（項目６０）
単一分子のアレイを製造するためのキットであって、
結合部位を有するアレイ、
各シードが単一の結合部位を有する、シード、および
該シードを構造化核酸粒子(SNAP)へと成長させるための試薬
を含む、キット。
（項目６１）
固体支持体；および
該固体支持体に直接結合しているポリマーベースの分子であって、該ポリマーベースの分子が、実質的に該固体支持体の反対側に配置されているタンパク質部分を含み、かつ、該タンパク質部分が親和性試薬にアクセス可能である、ポリマーベースの分子
を含む、組成物。
（項目６２）
アレイ上で生物学的または化学的実体を隔離する方法であって、
複数の構造化核酸粒子(SNAP)を作製する工程；
単一の生物学的または化学的実体を該複数のSNAPの各々にカップリングさせる工程；
該複数のSNAPをアレイに結合させる工程であって、該生物学的または化学的実体が実質的に該アレイの反対側にある、工程
を含み、
それによって、該複数のSNAPの各々の各生物学的または化学的実体を、該複数のSNAPの各SNAPのサイズに基づく距離だけ隔離する、方法。
（項目６３）
分子を分離する方法であって、各分子をより大きな荷電分子に変換する工程を含む、方法。
（項目６４）
各分子をより大きな荷電分子に変換する工程が、所望のサイズへと成長することができるバイオポリマーに該分子をコンジュゲートさせることを含む、項目63記載の方法。
（項目６５）
各分子をより大きな荷電分子に変換する工程が、各分子を10倍大きい分子に変換することを含む、項目63記載の方法。
（項目６６）
各分子をより大きな荷電分子に変換する工程が、各分子をより大きな荷電分子にコンジュゲートさせることを含む、項目63記載の方法。
（項目６７）
空間的に分離されている生物学的または化学的実体のアレイを製造する方法であって、
結合部位を有する固体支持体を得る工程、
複数の生物学的または化学的実体を含むサンプルを得る工程、
各々が官能基を有する構造化核酸粒子(SNAP)を得る工程、
該複数の生物学的または化学的実体の各生物学的または化学的実体を単一のSNAPに共有結合させる工程、
該SNAPを該アレイの該結合部位に結合させる工程、
を含み、
それによって、空間的に分離されている生物学的または化学的実体のアレイを製造する、方法。
（項目６８）
前記SNAPがローリングサークル増幅産物である、前記項目のいずれか一項記載の組成物。
（項目６９）
前記SNAPがプラスミドである、前記項目のいずれか一項記載の組成物。
（項目７０）
前記SNAPがDNA折り紙分子である、前記項目のいずれか一項記載の組成物。
（項目７１）
前記SNAPが核酸クラスターである、前記項目のいずれか一項記載の方法。
（項目７２）
前記SNAPがローリングサークル増幅産物である、前記項目のいずれか一項記載の方法。
（項目７３）
前記SNAPがプラスミドである、前記項目のいずれか一項記載の方法。
（項目７４）
前記SNAPがDNA折り紙分子である、前記項目のいずれか一項記載の方法。
（項目７５）
前記SNAPが核酸クラスターである、前記項目のいずれか一項記載の方法。
（項目７６）
前記SNAPがローリングサークル増幅産物である、前記項目のいずれか一項記載のキット。
（項目７７）
前記SNAPがプラスミドである、前記項目のいずれか一項記載のキット。
（項目７８）
前記SNAPがDNA折り紙分子である、前記項目のいずれか一項記載のキット。
（項目７９）
前記SNAPが核酸クラスターである、前記項目のいずれか一項記載のキット。
Although preferred embodiments of the invention have been shown and described herein, it will be apparent to those skilled in the art that such embodiments are provided merely by way of example. Numerous changes, changes and substitutions will now be conceived by those of skill in the art without departing from the present invention. It should be understood that various alternatives of aspects of the invention described herein can be used in the practice of the invention. The following claims define the scope of the invention, and it is intended that the methods and structures within the scope of this claim and their equivalents are protected by it.
In one aspect, the invention provides:
(Item 1)
A composition comprising structured nucleic acid particles (SNAPs) that are covalently attached to a protein.
(Item 2)
The composition according to item 1, wherein the SNAP is bound to a solid support.
(Item 3)
Item 2. The composition according to item 2, wherein the SNAP is covalently bonded to a solid support.
(Item 4)
The composition according to item 2, wherein the SNAP is non-covalently bonded to a solid support.
(Item 5)
A composition comprising the nucleic acid SNAP covalently attached to a biomolecule.
(Item 6)
5. The composition of item 5, wherein the SNAP is attached to a solid support.
(Item 7)
Item 6. The composition according to item 6, wherein the SNAP is covalently bonded to a solid support.
(Item 8)
6. The composition of item 6, wherein the SNAP is non-covalently attached to a solid support.
(Item 9)
A composition comprising a solid support attached to a plurality of structured nucleic acid particles (SNAPs), wherein each of the plurality of SNAPs is bound to a biomolecule.
(Item 10)
Item 9. The composition according to item 9, wherein the plurality of SNAPs are arranged in an array.
(Item 11)
A method of binding a single protein to a binding site on a solid support.
The binding site is larger than the protein;
The method is
A step of covalently binding the protein to a structured nucleic acid particle (SNAP), wherein the diameter of the SNAP is at least as large as the diameter of the binding site;
Step of binding the nucleic acid SNAP to the binding site
Including, how.
(Item 12)
11. The method of item 11, wherein each of the plurality of proteins binds to a binding site on the solid support such that each binding site binds to a single protein.
(Item 13)
A biomolecule array containing a plurality of biomolecules attached to a solid support, wherein each biomolecule of the plurality of biomolecules is covalently attached to a linker, and the linker is attached to the solid support. A biomolecule array in which each linker is attached to only one of the plurality of biomolecules, and each linker has a diameter of at least 50 nm.
(Item 14)
A method for producing an array of proteins that are spatially separated from a plurality of proteins, in which each protein of the plurality of proteins is covalently bonded to the terminal of a nucleic acid molecule constituting a structured nucleic acid particle (SNAP). , A method comprising the step of binding the SNAP to a solid support, thereby producing an array of spatially separated proteins.
(Item 15)
A composition comprising a protein, structured nucleic acid particles (SNAP), and a solid support, wherein the protein is covalently attached to the SNAP and the protein is not in contact with the solid support. ..
(Item 16)
A method of producing an array of spatially separated biological or chemical entities.
The process of obtaining a solid support with a binding site,
The process of obtaining a sample containing multiple biological or chemical entities,
Steps to obtain seeds, each with a functional group,
A step of covalently attaching each biological or chemical entity of the plurality of biological or chemical entities to a single seed via the functional group.
The process of growing each bound seed into structured nucleic acid particles (SNAP) of the desired size,
Step of binding the SNAP to the binding site of the array
Including
A method of producing an array of spatially separated biological or chemical entities.
(Item 17)
Item 16. The method according to item 16, wherein the solid support is a glass support.
(Item 18)
Item 16. The method according to item 16, wherein the solid support is a silica support.
(Item 19)
Item 16. The method according to item 16, wherein the solid support is a plastic support.
(Item 20)
Item 16. The method according to item 16, wherein the solid support is a silicon support.
(Item 21)
Item 16. The method according to item 16, wherein the solid support is a gold support.
(Item 22)
Item 16. The method according to item 16, wherein the solid support is a metal support.
(Item 23)
16. The method of item 16, wherein the solid support is a chrome support.
(Item 24)
Item 16. The method according to item 16, wherein the solid support is a titanium support.
(Item 25)
Item 16. The method according to item 16, wherein the solid support is a titanium oxide support.
(Item 26)
Item 16. The method according to item 16, wherein the solid support is a tin support.
(Item 27)
Item 16. The method according to item 16, wherein the solid support is a tin oxide support.
(Item 28)
16. The method of item 16, wherein the solid support is optically opaque.
(Item 29)
Item 16. The method of item 16, wherein the solid support is optically transparent.
(Item 30)
16. The method of item 16, wherein the solid support is modified to have a positive charge.
(Item 31)
16. The method of item 16, wherein the solid support is modified to have a negative charge.
(Item 32)
16. The method of item 16, wherein the solid support is modified to have a functional group capable of binding to the SNAP.
(Item 33)
16. The method of item 16, wherein the solid support comprises a binding site in which the solid support is modified to be different from the surrounding surface.
(Item 34)
27. The method of item 27, wherein the solid support comprises an array of binding sites.
(Item 35)
Item 34, wherein each binding site is at least 70 nm away from each of the other binding sites.
Law.
(Item 36)
34. The method of item 34, wherein each binding site is at least 25 nm away from each of the other binding sites.
(Item 37)
34. The method of item 34, wherein the distance between the ends of any two binding sites is greater than the radius of said SNAP used.
(Item 38)
34. The method of item 34, wherein the distance between the ends of any two binding sites is greater than the diameter of said SNAP used.
(Item 39)
16. The method of item 16, wherein the molecule is a protein.
(Item 40)
16. The method of item 16, wherein the seed is an oligonucleotide.
(Item 41)
40. The method of item 40, wherein the 3'end of the oligonucleotide is modified with a functional group.
(Item 42)
40. The method of item 40, wherein the 5'end of the oligonucleotide is modified with a functional group.
(Item 43)
The group in which the functional group consists of amine, thiol, carboxylic acid, triple bond, double bond, epoxide, alkyne, alkyne, cycloalkyne, azide, cyclooctyne, cycloalkyne, norbornen, tetradine, cyclooctane, epoxide, and hydroxyl. The method according to any one of items 41 or 42, which is selected from.
(Item 44)
40. The method of item 40, wherein the oligonucleotide is modified to contain a photocleavable bond.
(Item 45)
16. The method of item 16, wherein the SNAP is formed by rolling circle amplification.
(Item 46)
Item 16. The method according to item 16, wherein the SNAP is a dendrimer.
(Item 47)
46. The method of item 46, wherein the dendrimer is positively charged.
(Item 48)
47. The method of item 47, wherein the binding site on the array is negatively charged.
(Item 49)
46. The method of item 46, wherein the dendrimer is negatively charged.
(Item 50)
49. The method of item 49, wherein the binding site on the array is positively charged.
(Item 51)
50. The method of item 50, wherein the SNAP has a diameter of approximately 100 nm.
(Item 52)
45. The method of item 45, wherein the SNAP has a diameter of approximately 300 nm.
(Item 53)
45. The method of item 45, wherein the SNAP has a diameter of about 10 nm to 500 μm.
(Item 54)
45. The method of item 45, wherein the SNAP has a diameter of about 10 nm to 50 μm.
(Item 55)
45. The method of item 45, wherein the SNAP has a diameter of about 10 nm to 5 μm.
(Item 56)
45. The method of item 45, wherein the SNAP has a diameter of about 100 nm to 500 nm.
(Item 57)
45. The method of item 45, wherein the SNAP adheres to the solid support through electrostatic interaction.
(Item 58)
A way to achieve spatial separation of molecules,
The process of obtaining multiple molecules,
Steps to obtain seeds, each with a functional group,
A step of covalently attaching each of the plurality of molecules to a single seed via the functional group.
The step of growing each bound seed into structured nucleic acid particles (SNAP) of the desired size,
Step of binding the SNAP to a solid support
Including
A method that thereby achieves spatial separation of a single molecule.
(Item 59)
A single molecule array in which each single molecule is attached to a structured nucleic acid particle (SNAP) of the desired size, and the SNAP is attached to the array via the binding site. Array of.
(Item 60)
A kit for manufacturing single molecule arrays,
Arrays with binding sites,
Each seed has a single binding site, the seed, and
Reagents for growing the seed into structured nucleic acid particles (SNAP)
Including, kit.
(Item 61)
Solid support; and
A polymer-based molecule that is directly attached to the solid support, wherein the polymer-based molecule comprises a protein moiety that is located substantially opposite the solid support and the protein moiety. Are accessible to affinity reagents, polymer-based molecules
A composition comprising.
(Item 62)
A method of isolating biological or chemical entities on an array,
The process of producing multiple structured nucleic acid particles (SNAP);
The process of coupling a single biological or chemical entity to each of the multiple SNAPs;
A step of binding the plurality of SNAPs to an array, wherein the biological or chemical entity is substantially on the opposite side of the array.
Including
A method thereby isolating each biological or chemical entity of each of the plurality of SNAPs by a distance based on the size of each SNAP of the plurality of SNAPs.
(Item 63)
A method of separating molecules, comprising the step of converting each molecule into a larger charged molecule.
(Item 64)
63. The method of item 63, wherein the step of converting each molecule to a larger charged molecule comprises conjugating the molecule to a biopolymer capable of growing to a desired size.
(Item 65)
63. The method of item 63, wherein the step of converting each molecule to a larger charged molecule comprises converting each molecule into a 10-fold larger molecule.
(Item 66)
63. The method of item 63, wherein the step of converting each molecule to a larger charged molecule comprises conjugating each molecule to a larger charged molecule.
(Item 67)
A method of producing an array of spatially separated biological or chemical entities.
The process of obtaining a solid support with a binding site,
The process of obtaining a sample containing multiple biological or chemical entities,
Steps to obtain structured nucleic acid particles (SNAP), each with a functional group,
The step of covalently binding each biological or chemical entity of the plurality of biological or chemical entities to a single SNAP.
The step of binding the SNAP to the binding site of the array,
Including
A method of producing an array of spatially separated biological or chemical entities.
(Item 68)
The composition according to any one of the above items, wherein the SNAP is a rolling circle amplification product.
(Item 69)
The composition according to any one of the above items, wherein the SNAP is a plasmid.
(Item 70)
The composition according to any one of the above items, wherein the SNAP is a DNA origami molecule.
(Item 71)
The method according to any one of the above items, wherein the SNAP is a nucleic acid cluster.
(Item 72)
The method according to any one of the above items, wherein the SNAP is a rolling circle amplification product.
(Item 73)
The method according to any one of the above items, wherein the SNAP is a plasmid.
(Item 74)
The method according to any one of the above items, wherein the SNAP is a DNA origami molecule.
(Item 75)
The method according to any one of the above items, wherein the SNAP is a nucleic acid cluster.
(Item 76)
The kit according to any one of the above items, wherein the SNAP is a rolling circle amplification product.
(Item 77)
The kit according to any one of the above items, wherein the SNAP is a plasmid.
(Item 78)
The kit according to any one of the above items, wherein the SNAP is a DNA origami molecule.
(Item 79)
The kit according to any one of the above items, wherein the SNAP is a nucleic acid cluster.
Claims (15)
該方法が、
該単一のタンパク質を構造化核酸粒子(SNAP)に共有結合させる工程であって、該SNAPの直径が少なくとも該結合部位の直径と同じ大きさである、工程；および
該結合部位が該単一のタンパク質に結合するように、該SNAPを該結合部位に結合させる工程
を含む、方法。 A method of binding a single protein to a binding site on a solid support.
The method is
A step of covalently binding the single protein to a structured nucleic acid particle (SNAP), wherein the diameter of the SNAP is at least as large as the diameter of the binding site;
A method comprising binding the SNAP to the binding site such that the binding site binds to the single protein .
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Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4365597A2 (en) | 2016-12-01 | 2024-05-08 | Nautilus Subsidiary, Inc. | Methods of assaying proteins |
| WO2019036055A2 (en) | 2017-08-18 | 2019-02-21 | Ignite Biosciences, Inc. | Methods of selecting binding reagents |
| JP7434161B2 (en) * | 2017-10-23 | 2024-02-20 | ノーティラス・サブシディアリー・インコーポレイテッド | Methods and systems for protein identification |
| WO2019195633A1 (en) | 2018-04-04 | 2019-10-10 | Ignite Biosciences, Inc. | Methods of generating nanoarrays and microarrays |
| AU2020266136A1 (en) * | 2019-04-29 | 2021-11-25 | Nautilus Subsidiary, Inc. | Methods and systems for integrated on-chip single-molecule detection |
| CN114072499A (en) * | 2019-04-30 | 2022-02-18 | Encodia 公司 | Method for preparing an analyte and related kit |
| CN114207148A (en) * | 2019-12-23 | 2022-03-18 | 伊鲁米纳公司 | Nanoparticles with a single site for template polynucleotide attachment |
| AU2021288692A1 (en) | 2020-06-11 | 2023-02-02 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| CA3196729A1 (en) | 2020-11-11 | 2022-05-19 | Tural AKSEL | Affinity reagents having enhanced binding and detection characteristics |
| EP4281774A2 (en) | 2021-01-20 | 2023-11-29 | Nautilus Subsidiary, Inc. | Methods for biomolecule quantitation |
| US20220227890A1 (en) | 2021-01-21 | 2022-07-21 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule preparation |
| US11505796B2 (en) | 2021-03-11 | 2022-11-22 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule retention |
| WO2023038859A1 (en) | 2021-09-09 | 2023-03-16 | Nautilus Biotechnology, Inc. | Characterization and localization of protein modifications |
| WO2023049073A1 (en) | 2021-09-22 | 2023-03-30 | Nautilus Biotechnology, Inc. | Methods and systems for determining polypeptide interactions |
| CA3232183A1 (en) | 2021-10-11 | 2023-04-20 | Nautilus Subsidiary, Inc. | Highly multiplexable analysis of proteins and proteomes |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| CN114235988B (en) * | 2021-11-27 | 2023-12-22 | 山东省烟台市农业科学研究院 | High performance liquid chromatography for determining content of anti-sprouting pellet |
| US20230167488A1 (en) * | 2021-11-30 | 2023-06-01 | Nautilus Biotechnology, Inc. | Particle-based isolation of proteins and other analytes |
| WO2023114732A2 (en) | 2021-12-14 | 2023-06-22 | Glyphic Biotechnologies, Inc. | Single-molecule peptide sequencing through molecular barcoding and ex-situ analysis |
| US20230314324A1 (en) | 2022-03-29 | 2023-10-05 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| WO2023196642A1 (en) | 2022-04-08 | 2023-10-12 | Glyphic Biotechnologies, Inc. | Methods and systems for processing polymeric analytes |
| WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| WO2023250364A1 (en) | 2022-06-21 | 2023-12-28 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024030919A1 (en) | 2022-08-02 | 2024-02-08 | Glyphic Biotechnologies, Inc. | Protein sequencing via coupling of polymerizable molecules |
| US20240053333A1 (en) | 2022-08-03 | 2024-02-15 | Nautilus Subsidiary, Inc. | Chemical modification of antibodies and functional fragments thereof |
| WO2024058967A1 (en) | 2022-09-13 | 2024-03-21 | Nautilus Subsidiary, Inc. | Systems and methods of validating new affinity reagents |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024072614A1 (en) | 2022-09-27 | 2024-04-04 | Nautilus Subsidiary, Inc. | Polypeptide capture, in situ fragmentation and identification |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| CN116732141B (en) * | 2023-07-10 | 2024-04-02 | 海南大学 | Method for rapidly detecting specificity of biological DNA |
| CN117723749A (en) * | 2024-02-07 | 2024-03-19 | 南昌大学 | Dynamic light scattering immunosensory detection method based on molecular adhesive |
Family Cites Families (88)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060194258A1 (en) * | 1989-06-07 | 2006-08-31 | Affymetrix, Inc. | Polypeptide array synthesis |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US20040132067A1 (en) * | 1990-06-11 | 2004-07-08 | Somalogic, Inc. | Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex |
| US5635602A (en) * | 1993-08-13 | 1997-06-03 | The Regents Of The University Of California | Design and synthesis of bispecific DNA-antibody conjugates |
| US6682886B1 (en) | 1994-04-28 | 2004-01-27 | Gilead Sciences, Inc. | Bivalent binding molecules of 7 transmembrane G protein-coupled receptors |
| WO1998030575A1 (en) | 1997-01-08 | 1998-07-16 | Proligo Llc | Bioconjugation of macromolecules |
| US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
| US6255469B1 (en) | 1998-05-06 | 2001-07-03 | New York University | Periodic two and three dimensional nucleic acid structures |
| US20020197656A1 (en) * | 1999-12-17 | 2002-12-26 | Ronghao Li | Cell arrays and the uses thereof |
| US6500611B2 (en) * | 2000-08-17 | 2002-12-31 | Thomas L. Mattson | Inviable virus particles as scaffolds for constructing flexible detection systems |
| WO2002086081A2 (en) | 2001-04-20 | 2002-10-31 | Carnegie Mellon University | Methods and systems for identifying proteins |
| WO2003093168A2 (en) * | 2001-07-26 | 2003-11-13 | Motorola, Inc. | System and methods for mixing within a microfluidic device |
| US6720595B2 (en) | 2001-08-06 | 2004-04-13 | International Business Machines Corporation | Three-dimensional island pixel photo-sensor |
| AU2002351164A1 (en) | 2001-11-26 | 2003-06-10 | Molecular Reflections, Inc. | Microscale immobilization of molecules using a hydrogel and methods of use thereof |
| AU2003240482B2 (en) | 2002-05-30 | 2009-03-12 | The Scripps Research Institute | Copper-catalysed ligation of azides and acetylenes |
| US6998241B2 (en) | 2002-09-11 | 2006-02-14 | Kimberly-Clark Worldwide, Inc. | Antibody pair screening methods |
| WO2004027093A1 (en) * | 2002-09-19 | 2004-04-01 | The Chancellor, Master And Scholars Of The University Of Oxford | Molecular arrays and single molecule detection |
| WO2004035742A2 (en) | 2002-10-15 | 2004-04-29 | Abmetrix, Inc. | Sets of digital antibodies directed against short epitopes, and methods using same |
| AU2003293748A1 (en) * | 2002-12-04 | 2004-06-23 | Pamgene Bv | Method for hybridisation of immobilized genomic dna |
| WO2004094456A2 (en) * | 2003-04-18 | 2004-11-04 | Becton, Dickinson And Company | Immuno-amplification |
| US7259258B2 (en) | 2003-12-17 | 2007-08-21 | Illumina, Inc. | Methods of attaching biological compounds to solid supports using triazine |
| US20050153321A1 (en) * | 2003-12-30 | 2005-07-14 | Saba James A. | Libraries of multiple-ligand-conjugated nucleic acids |
| EP2789383B1 (en) | 2004-01-07 | 2023-05-03 | Illumina Cambridge Limited | Molecular arrays |
| JP2008504846A (en) | 2004-06-10 | 2008-02-21 | ニューヨーク ユニバーシティ | Lattice assemblies based on polygonal nanostructures and double cross-linking of polynucleic acid multi-crossover molecules |
| DE102004057430A1 (en) * | 2004-11-27 | 2006-06-01 | Degussa Ag | Polymeric nanocomposite materials by controlled nucleation of dendritic polymers |
| US20060160234A1 (en) | 2005-01-18 | 2006-07-20 | Viorica Lopez-Avila | Methods for assessing polypeptide array quality |
| CA2601394A1 (en) | 2005-03-17 | 2006-09-28 | Primex Clinical Laboratories, Inc. | Immunogens for vaccines against antigenically variable pathogens and diseases |
| WO2006122002A2 (en) * | 2005-05-09 | 2006-11-16 | Panomics, Inc. | Multiplex capture of nucleic acids |
| EP1880019A1 (en) | 2005-05-12 | 2008-01-23 | Arizona Board Regents, a body corporate of the State of Arizona, acting for and on behalf of Arizona State University | Self-assembled nucleic acid nanoarrays and uses therefor |
| WO2006135527A2 (en) | 2005-06-10 | 2006-12-21 | Saint Louis University | Methods for the selection of aptamers |
| US7842793B2 (en) | 2005-06-14 | 2010-11-30 | The California Institute Of Technology | Methods of making nucleic acid nanostructures |
| DK1907571T3 (en) * | 2005-06-15 | 2017-08-21 | Complete Genomics Inc | NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS |
| US20070248546A1 (en) * | 2005-11-09 | 2007-10-25 | Exact Sciences Corporation | Clinical algorithm for excluding patients identified in virtual imaging |
| WO2007120208A2 (en) * | 2005-11-14 | 2007-10-25 | President And Fellows Of Harvard College | Nanogrid rolling circle dna sequencing |
| US7754475B2 (en) * | 2006-01-25 | 2010-07-13 | Agilent Technologies, Inc. | Nucleic acid probes and microarrays for analysis of polynucleotides |
| US20070218503A1 (en) | 2006-02-13 | 2007-09-20 | Mitra Robi D | Methods of polypeptide identification, and compositions therefor |
| US7692783B2 (en) | 2006-02-13 | 2010-04-06 | Pacific Biosciences Of California | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
| CN100500865C (en) | 2006-04-21 | 2009-06-17 | 华东师范大学 | Method for detecting CatD protein by identifying a plurality of epitopes synchronously |
| WO2007117444A2 (en) | 2006-03-31 | 2007-10-18 | Yinghe Hu | Protein detection by aptamers |
| WO2009026313A1 (en) * | 2007-08-20 | 2009-02-26 | Colorado Seminary, Which Owns And Operates The University Of Denver | Photoinduced signal amplification through externally sensitized photofragmentation in masked photosensitizers and photoamplified fluorescence turn-off system |
| CA2720587A1 (en) | 2008-04-09 | 2009-10-15 | Francis Barany | Coferons and methods of making and using them |
| US9102520B2 (en) | 2008-08-13 | 2015-08-11 | California Institute Of Technology | Nanocomposite structures and related methods and systems |
| WO2010065531A1 (en) | 2008-12-01 | 2010-06-10 | Robi David Mitra | Single molecule protein screening |
| US8603792B2 (en) * | 2009-03-27 | 2013-12-10 | Life Technologies Corporation | Conjugates of biomolecules to nanoparticles |
| WO2010120385A1 (en) * | 2009-04-18 | 2010-10-21 | Massachusetts Institute Of Technology | pH SENSITIVE BIODEGRADABLE POLYMERIC PARTICLES FOR DRUG DELIVERY |
| US9523680B2 (en) * | 2010-06-30 | 2016-12-20 | Ambergen, Inc. | Global Proteomic screening of random bead arrays using mass spectrometry imaging |
| US9671344B2 (en) * | 2010-08-31 | 2017-06-06 | Complete Genomics, Inc. | High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging |
| CN102618940A (en) | 2011-01-31 | 2012-08-01 | 艾比玛特生物医药（上海）有限公司 | Antibody preparation method and obtained antibody and antibody database |
| CA2849072A1 (en) | 2011-08-05 | 2013-02-14 | President And Fellows Of Harvard College | Compositions and methods relating to nucleic acid nano-and micro-technology |
| JP6093498B2 (en) * | 2011-12-13 | 2017-03-08 | 株式会社日立ハイテクノロジーズ | Nucleic acid amplification method |
| WO2013119676A1 (en) | 2012-02-06 | 2013-08-15 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University | Novel dna-origami nanovaccines |
| WO2013148186A1 (en) | 2012-03-26 | 2013-10-03 | President And Fellows Of Harvard College | Lipid-coated nucleic acid nanostructures of defined shape |
| NL2009191C2 (en) | 2012-07-16 | 2014-01-20 | Univ Delft Tech | Single molecule protein sequencing. |
| WO2014018675A1 (en) | 2012-07-24 | 2014-01-30 | President And Fellows Of Harvard College | Self-assembly of nucleic acid nanostructures |
| US9975916B2 (en) | 2012-11-06 | 2018-05-22 | President And Fellows Of Harvard College | Compositions and methods relating to complex nucleic acid nanostructures |
| EP4012716A1 (en) | 2012-11-19 | 2022-06-15 | Apton Biosystems, Inc. | Digital analysis of molecular analytes using single molecule detection |
| US10829816B2 (en) | 2012-11-19 | 2020-11-10 | Apton Biosystems, Inc. | Methods of analyte detection |
| DK3087083T3 (en) | 2013-12-27 | 2019-12-16 | Hoffmann La Roche | Systemic discovery, maturation and extension of peptide binders to proteins |
| US9275871B2 (en) | 2014-01-09 | 2016-03-01 | Micron Technology, Inc. | Nanostructures having low defect density and methods of forming thereof |
| WO2015172080A1 (en) * | 2014-05-08 | 2015-11-12 | Fluidigm Corporation | Integrated single cell sequencing |
| US10099920B2 (en) | 2014-05-22 | 2018-10-16 | President And Fellows Of Harvard College | Scalable nucleic acid-based nanofabrication |
| JP2017536091A (en) | 2014-10-02 | 2017-12-07 | シティ・オブ・ホープＣｉｔｙ ｏｆ Ｈｏｐｅ | Multivalent meditope, meditope-binding antibody and use thereof |
| EP3009520B1 (en) * | 2014-10-14 | 2018-12-12 | Karlsruher Institut für Technologie | Site-specific immobilization of DNA origami structures on solid substrates |
| US10550145B2 (en) | 2015-03-07 | 2020-02-04 | President And Fellows Of Harvard College | Single-stranded DNA nanostructures |
| WO2016154345A1 (en) * | 2015-03-24 | 2016-09-29 | Pacific Biosciences Of California, Inc. | Methods and compositions for single molecule composition loading |
| US9330932B1 (en) | 2015-03-31 | 2016-05-03 | Micron Technology, Inc. | Methods of fabricating features associated with semiconductor substrates |
| US9466504B1 (en) | 2015-03-31 | 2016-10-11 | Micron Technology, Inc. | Methods of fabricating features associated with semiconductor substrates |
| US9881786B2 (en) | 2015-04-02 | 2018-01-30 | Micron Technology, Inc. | Methods of forming nanostructures using self-assembled nucleic acids, and nanostructures thereof |
| WO2016174525A1 (en) * | 2015-04-29 | 2016-11-03 | Biosims Technologies | Enhanced sensitivity in ligand binding assays performed with secondary ion mass spectrometry |
| EP3405575A4 (en) | 2016-01-20 | 2019-06-26 | Vitrisa Therapeutics, Inc. | Methods for improved aptamer selection |
| US11168320B2 (en) | 2016-08-09 | 2021-11-09 | Arizona Board Of Regents On Behalf Of Arizona State University | Structure assisted directed evolution of multivalent aptamers |
| EP4365597A2 (en) | 2016-12-01 | 2024-05-08 | Nautilus Subsidiary, Inc. | Methods of assaying proteins |
| WO2019036055A2 (en) | 2017-08-18 | 2019-02-21 | Ignite Biosciences, Inc. | Methods of selecting binding reagents |
| US11125748B2 (en) | 2017-09-01 | 2021-09-21 | University Of British Columbia | Method for organizing individual molecules on a patterned substrate and structures assembled thereby |
| US11391734B2 (en) | 2017-09-25 | 2022-07-19 | California Institute Of Technology | Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events |
| WO2019059961A1 (en) | 2017-09-25 | 2019-03-28 | California Institute Of Technology | Bistable polynucleotide devices for the sensing and quantification of molecular events |
| US11721412B2 (en) | 2017-10-23 | 2023-08-08 | Nautilus Subsidiary, Inc. | Methods for identifying a protein in a sample of unknown proteins |
| JP7434161B2 (en) | 2017-10-23 | 2024-02-20 | ノーティラス・サブシディアリー・インコーポレイテッド | Methods and systems for protein identification |
| KR102556494B1 (en) * | 2017-10-31 | 2023-07-18 | 엔코디아, 인코포레이티드 | Kits for assays using nucleic acid encoding and/or labeling |
| AU2018358247A1 (en) | 2017-10-31 | 2020-05-21 | Encodia, Inc. | Methods and kits using nucleic acid encoding and/or label |
| WO2019108954A1 (en) | 2017-12-01 | 2019-06-06 | Arizona Board Of Regents On Behalf Of Arizona State University | Materials and methods relating to single molecule arrays |
| WO2019195633A1 (en) | 2018-04-04 | 2019-10-10 | Ignite Biosciences, Inc. | Methods of generating nanoarrays and microarrays |
| GB201807409D0 (en) | 2018-05-04 | 2018-06-20 | Oxford Nanoimaging Ltd | Assay |
| WO2019236749A2 (en) | 2018-06-06 | 2019-12-12 | Ignite Biosciences, Inc. | Methods and applications of protein identification |
| WO2020106889A1 (en) | 2018-11-20 | 2020-05-28 | Nautilus Biotechnology, Inc. | Design and selection of affinity reagents |
| AU2020266136A1 (en) | 2019-04-29 | 2021-11-25 | Nautilus Subsidiary, Inc. | Methods and systems for integrated on-chip single-molecule detection |
| US20220339181A1 (en) | 2019-06-21 | 2022-10-27 | Tilibit Nanosystems Gmbh | Conditionally switchable nanostructure |
| US20220379582A1 (en) | 2019-10-30 | 2022-12-01 | Nautilus Biotechnology, Inc. | Flow cell systems and methods |
-
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- 2019-04-04 WO PCT/US2019/025909 patent/WO2019195633A1/en unknown
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