JPWO2019161141A5 - - Google Patents

Claims (17)

A method of producing one or more terpenes, the method comprising culturing a host cell containing a nucleic acid molecule encoding a chimeric terpene synthase, wherein the host cell comprises the following terpenes: alpha-gueien, delta. -A method for producing one or more of cadinenes, cis-eudesmu-6-en-11-ol, beta-caryophyllene, humulene, and / or alpha-casinohl. The chimeric terpene synthase contains sequences from at least two terpene synthases, at least one of the terpene synthases is a plant terpene synthase, and optionally the plants are Hibiscadelphus wilderianus, Leucadendron grandiflorum, Macrostylis villosa, Orbexilum stipulatum, Shorea cuspidata. , And the method of claim 1, selected from the group consisting of Wendlandia angustifolia. The chimeric terpene synthase is an alpha-guien synthase comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 17, 22, or 29, and optionally the alpha-guien synthase is SEQ ID NO: 178, 183, and 211-. The method of claim 1 or 2, comprising one or more sequences derived from a plant terpene synthase selected from the group consisting of 214. The chimeric terpene synthase produces delta-cadinene, the chimeric terpene synthase contains an amino acid sequence that is at least 90% identical to SEQ ID NO: 40, 41, 42, 44, 45, or 47, and optionally the chimeric terpene synthase. For terpene synthase in plants selected from the group consisting of SEQ ID NOs: 261, 262, 263, 264, 265, 267, 268, 271, 275, 276, 279, 296, 301, 306, 307, 308, 324, and 325. The method of claim 1 or 2, comprising one or more sequences from which it is derived. The chimeric terpene synthase produces cis-eudesm-6-en-11-ol, and the chimeric terpene synthase is an amino acid that is at least 90% identical to SEQ ID NO: 36, 37, 43, 46, 48, 49, 50, or 51. Containing the sequence, optionally, the chimeric terpene synthase comprises SEQ ID NOs: 245, 246, 247, 248, 249, 255, 256, 257, 258, 290, 291, 292, 329, 337, 338, 343, and 349. The method of claim 1 or 2, comprising one or more sequences derived from a plant terpene synthase selected from the group. The chimeric terpene synthase produces beta-caryophyllene and / or humulene, the chimeric terpene synthase comprises at least 90% identical amino acid sequence to SEQ ID NO: 23, 24, 25, or 26, and optionally the chimeric terpene synthase. Claim 1 or 2 comprising one or more sequences derived from the terpene synthase of a plant selected from the group consisting of SEQ ID NOs: 184, 185, 186, 187, 188, 190, 191, 194, 195, 196, and 197. The method described in. The chimeric terpene synthase produces alpha-casinolu, the chimeric terpene synthase contains at least 90% identical amino acid sequence to any one of SEQ ID NOs: 30-35, and optionally the chimeric terpene synthase is SEQ ID NO: 215, 216. 217, 218, 219, 221, 222, 223, 228, 229, 234, and 237. Method. The chimeric terpene synthase produces delta-cadinene, the chimeric terpene synthase contains an amino acid sequence that is at least 98% identical to SEQ ID NO: 1, 3, 4, 5, 6, 7, or 12, and optionally the chimeric terpene synthase. 1 comprises one or more sequences derived from a plant terpene synthase selected from the group consisting of SEQ ID NOs: 119, 120, 121, 122, 123, 124, 125, 139, 140, 141, and 172. Or the method according to 2. The chimeric terpene synthase produces delta-cadinene, the chimeric terpene synthase contains at least 97% identical amino acid sequence to SEQ ID NO: 11, 18, or 19, and optionally the chimeric terpene synthase is SEQ ID NO: 171 and 179. The method of claim 1 or 2, comprising one or more sequences derived from a plant terpene synthase selected from the group consisting of and 180. The host cell is a fungal cell, a plant cell, or a bacterial cell, optionally the fungal cell is a yeast cell, and optionally the yeast cell is a budding yeast or a yeast cell of the genus Yeast, and more optionally. The method according to any one of claims 1 to 9, wherein the sprouting yeast cell is a Saccharomyces cerevisiae cell. The method according to any one of claims 1 to 10, further comprising extracting one or more terpenes. The method according to any one of claims 1 to 11, wherein at least one of the one or more terpenes is an aromatic compound. A host cell containing a nucleic acid molecule encoding a chimeric terpene synthase, wherein at least 10% of the amino acid sequence of the chimeric terpene synthase is derived from an extinct plant. A method of producing an aromatic compound comprising culturing a host cell containing a nucleic acid molecule encoding a chimeric terpene synthase, wherein the chimeric terpene synthase comprises a sequence derived from at least two terpene synthases and comprises a sequence of terpene synthase. A method in which at least one is derived from an extinct plant. At least 10% of the amino acid sequence of the chimeric terpene synthase is derived from an extinct plant, and optionally the chimeric terpene synthase comprises at least 90% identical amino acid sequence to the amino acid selected from the group consisting of SEQ ID NOs: 1-52. , Chimera terpene synthase. The nucleic acid molecule encoding the chimeric terpene synthase according to claim 15, wherein the nucleic acid molecule optionally comprises a sequence that is at least 90% identical to the nucleotide sequence selected from the group consisting of SEQ ID NOs: 67-118. A chimeric terpene synthase comprising a sequence selected from the group consisting of SEQ ID NOs: 119-357.