JPWO2011158852A1 - 誘導型多能性幹細胞の製造方法 - Google Patents

誘導型多能性幹細胞の製造方法 Download PDF

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Publication number
JPWO2011158852A1
JPWO2011158852A1 JP2012520466A JP2012520466A JPWO2011158852A1 JP WO2011158852 A1 JPWO2011158852 A1 JP WO2011158852A1 JP 2012520466 A JP2012520466 A JP 2012520466A JP 2012520466 A JP2012520466 A JP 2012520466A JP WO2011158852 A1 JPWO2011158852 A1 JP WO2011158852A1
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cell
cells
protein
reprogramming factor
pluripotent stem
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Pending
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English (en)
Japanese (ja)
Inventor
内 啓 光 中
内 啓 光 中
津 真 大
津 真 大
山 直 也 高
山 直 也 高
東 赫 安
東 赫 安
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University of Tokyo NUC
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University of Tokyo NUC
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/602Sox-2
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/604Klf-4
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/606Transcription factors c-Myc
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012520466A 2010-06-15 2011-06-15 誘導型多能性幹細胞の製造方法 Pending JPWO2011158852A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35484810P 2010-06-15 2010-06-15
US61/354,848 2010-06-15
PCT/JP2011/063650 WO2011158852A1 (fr) 2010-06-15 2011-06-15 Procédé de production de cellules souches pluripotentes induites

Publications (1)

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JPWO2011158852A1 true JPWO2011158852A1 (ja) 2013-08-19

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JP2012520466A Pending JPWO2011158852A1 (ja) 2010-06-15 2011-06-15 誘導型多能性幹細胞の製造方法

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JP (1) JPWO2011158852A1 (fr)
WO (1) WO2011158852A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150011393A1 (en) * 2012-02-08 2015-01-08 National University Corporation NARA Institute of Science and Technology Method for introducing florigen
EP3604499A1 (fr) 2014-03-04 2020-02-05 Fate Therapeutics, Inc. Procédés améliorés de reprogrammation et plateformes de culture cellulaire
US10138469B2 (en) 2014-06-23 2018-11-27 Toagosei Co., Ltd. Synthetic peptide and use thereof
US11441126B2 (en) 2015-10-16 2022-09-13 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009032194A1 (fr) * 2007-08-31 2009-03-12 Whitehead Institute For Biomedical Research Stimulation de la voie wnt dans la reprogrammation de cellules somatiques
WO2009067757A1 (fr) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Méthodes permettant d'induire la pluripotence impliquant la protéine oct4
WO2009067756A1 (fr) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Méthodes d'induction de la pluripotence impliquant la protéine sox2
JP2010110289A (ja) * 2008-11-10 2010-05-20 Univ Of Tokyo iPS細胞の樹立法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009032194A1 (fr) * 2007-08-31 2009-03-12 Whitehead Institute For Biomedical Research Stimulation de la voie wnt dans la reprogrammation de cellules somatiques
WO2009067757A1 (fr) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Méthodes permettant d'induire la pluripotence impliquant la protéine oct4
WO2009067756A1 (fr) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Méthodes d'induction de la pluripotence impliquant la protéine sox2
JP2010110289A (ja) * 2008-11-10 2010-05-20 Univ Of Tokyo iPS細胞の樹立法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JPN6014022528; KIM,D. et al.: 'Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.' Cell Stem Cell Vol.4, No.6, 20090605, pp.472-6 *
JPN6014022529; ZHOU,H. et al.: 'Generation of induced pluripotent stem cells using recombinant proteins.' Cell Stem Cell Vol.4, No.5, 20090508, pp.381-4 *
JPN6014022532; FLINTERMAN,M. et al.: 'Delivery of therapeutic proteins as secretable TAT fusion products.' Mol. Ther. Vol.17, No.2, 200902, pp.334-42 *

Also Published As

Publication number Publication date
WO2011158852A1 (fr) 2011-12-22

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