JPWO2009113487A1 - Micro inspection chip and liquid dividing method of micro inspection chip - Google Patents

Abstract

Divide the liquid into multiple parts by flowing the liquid stored in the liquid reservoir provided at the liquid inlet through the multiple connection ports provided in the liquid reservoir or directly into the multiple liquid supply channels. can do. Further, by sealing the plurality of connection ports with the protrusions provided on the lid that seals the liquid injection port, communication between the plurality of liquid supply channels can be prevented. Thereby, a micro inspection chip and a microchip that can divide and inject a small amount of liquid into a plurality of liquid supply flow paths with a simple operation with high accuracy and can stably supply liquid after divided injection. The liquid dividing method can be provided.

Description

  The present invention relates to a microinspection chip and a method for dividing a liquid into a microinspection chip, and in particular, a target compound by inspecting / analyzing biological substances by gene amplification reaction, antigen-antibody reaction, etc., inspecting / analyzing other chemical substances, organic synthesis The present invention relates to a micro inspection chip used for chemical synthesis of the liquid and a liquid dividing method of the micro inspection chip.

  In recent years, by making full use of micromachine technology and ultrafine processing technology, devices and means (for example, pumps, valves, flow paths, sensors, etc.) for performing conventional sample preparation, chemical analysis, chemical synthesis, etc. have been miniaturized. An analysis chip (hereinafter referred to as a micro inspection chip) integrated on a chip has been developed (see, for example, Patent Document 1).

  This is also called μ-TAS (Micro Total Analysis System), bioreactor, Lab-on-chip, biochip, and it is used in the medical examination / diagnosis field, environmental measurement field, and agricultural production field. Application is expected. In particular, when complicated processes such as those found in genetic testing, skilled techniques, and operation of equipment are required, micro test chips that excel in automation, speed-up, and simplification are cost-effective and require sample volume. Because it enables analysis not only for the required time but also for any time and place, the benefits are great.

  The present applicant injects a specimen such as blood into a micro test chip enclosing a reagent, etc., and injects a driving liquid with a micro pump into the micro flow path of the micro test chip, thereby moving the specimen and reacting sequentially. Have proposed a reaction detection device capable of measuring the results (see, for example, Patent Document 2).

In the micro test chip as described above, a sample such as blood used for the test or a liquid such as a reagent for the test is divided into a plurality according to the test item and micro test is performed from the injection port provided in the micro test chip. It is necessary to inject into the chip. Injecting a specimen into such a micro test chip has been performed by a person in charge of the test using a dropper or pipette to inject the sample into the injection port of the micro test chip.
JP 2004-28589 A JP 2006-149379 A

  In general genetic diagnosis, even in the case of single item diagnosis, the condition of the reagent and the inhibitor contained in the sample may affect the diagnosis result. A method is often used in which a minute amount is divided into a plurality of flow paths and the reliability of diagnosis is ensured by comparison with a reference reaction. For this purpose, it is necessary to divide a liquid such as a specimen into a plurality of parts.

  For example, as shown in FIG. 6A, the liquid injected into the liquid reservoir 111 from one injection port 113 is sent downstream by the driving liquid injected from the connection port 117 with the micropump, A method of dividing into a plurality of division channels 115 (here, division into two) is conceivable.

  However, in this case, since the flow path 115a and the flow path 115b to which the divided liquid is fed are communicated with each other through the divided flow path 115, the force of the micropumps at the time of liquid feeding is mutually There is a problem in that stable liquid feeding cannot be performed and measurement accuracy at the time of division is reduced. Further, in the case of multi-item diagnosis, since it is necessary to divide the liquid into a larger number, it is considered that a more complicated division and a flow path with communication are necessary, and the micro inspection chip 100 is enlarged, and the original small size, The merit of μ-TAS, which is quick and highly accurate, cannot be utilized.

  Further, for example, as shown in FIG. 6 (b), the person in charge of inspection uses a manual pipette or the like to supply liquid from a plurality of (for example, two places) inlets (for example, 113a and 113b) to respective liquid storage portions (for example, 111a and A method of injecting into 111b) is also conceivable. In this case, there is no influence of communication in the divided flow path 115 described above.

  However, especially when dealing with multi-item diagnosis, the number of injections increases, which is very time-consuming, and the work becomes complicated, so there is a high probability that an operation error (mixing of bubbles, excessive injection amount) will occur. Decreasing usability, such as reducing diagnostic accuracy, is a major problem. Furthermore, since the injection port generally requires a larger space than a normal flow path, if a large number of injection ports are provided, the micro inspection chip becomes large, and the original small, quick and high-precision μ-TAS. The benefits of will be lost.

  The present invention has been made in view of the above problems, and a minute amount of liquid can be divided and injected into a plurality of liquid feeding channels with a simple operation with high accuracy, and is stable after divided injection. It is an object of the present invention to provide a micro inspection chip and a liquid separation method of a micro chip that can be fed in a liquid state.

  The object of the present invention can be achieved by the following constitution.

1. In a micro test chip comprising a liquid inlet for injecting a liquid and a plurality of liquid supply channels for supplying the injected liquid,
The liquid inlet has a lid that seals the liquid inlet, a holding portion that holds the lid, and a liquid reservoir that temporarily stores liquid,
The liquid reservoir has a plurality of connection ports communicated with each of the plurality of liquid supply passages,
The lid has a protrusion that seals the plurality of connection ports and prevents communication between the plurality of liquid supply channels when the lid is inserted into the holding portion. Inspection chip.

2. The protruding portion has a shape that is in close contact with all or a part of the liquid reservoir when the lid is inserted into the holding portion.
By inserting the lid into the holding part,
2. The micro test chip according to 1 above, wherein the protrusion drops the liquid dropped on the liquid reservoir into a plurality of the connection ports, and divides the liquid into a plurality of flow paths. .

3. In a micro test chip comprising a liquid inlet for injecting a liquid and a plurality of liquid supply channels for supplying the injected liquid,
The liquid inlet has a lid that seals the liquid inlet, a holding portion that holds the lid, and a liquid reservoir that temporarily stores liquid,
The plurality of liquid supply passages are disposed at the lower part of the liquid reservoir, and a part or all of each is open on the bottom surface of the liquid reservoir,
The lid includes a protrusion that seals the plurality of liquid supply channels and prevents communication between the plurality of liquid channels when the lid is inserted into the holding unit. Micro inspection chip.

4). The protruding portion has a shape that is in close contact with all or a part of the liquid reservoir when the lid is inserted into the holding portion.
By inserting the lid into the holding part,
4. The micro test chip according to 3 above, wherein the protrusion drops the liquid dropped into the liquid reservoir part into a plurality of parts and flows into the plurality of liquid supply channels.

  5. 5. The micro test chip according to 3 or 4, wherein the liquid reservoir and the wall surfaces of the plurality of liquid supply channels have hydrophilicity.

6). In the liquid dividing method using the micro inspection chip according to any one of 1 to 5,
A dropping step of dropping a liquid into the liquid reservoir;
An insertion step of inserting the lid into the holding portion;
A dividing step of dividing the liquid into the plurality of liquid feeding flow paths by the protrusions;
A liquid dividing method for a micro test chip, comprising: a separation step of sealing a plurality of the liquid supply flow paths to prevent communication between the plurality of liquid supply flow paths.

  According to the present invention, the liquid stored in the liquid reservoir provided in the liquid inlet is poured into the plurality of liquid supply channels through the plurality of connection ports provided in the liquid reservoir or directly. The liquid can be divided into a plurality of pieces. Further, by sealing the plurality of connection ports or the plurality of liquid supply channels with the protrusions provided on the lid that seals the liquid injection port, communication between the plurality of liquid supply channels can be prevented. Thereby, a micro inspection chip and a microchip that can divide and inject a small amount of liquid into a plurality of liquid supply flow paths with a simple operation with high accuracy and can stably supply liquid after divided injection. The liquid dividing method can be provided.

It is a perspective view which shows an example of an inspection apparatus. It is a block diagram which shows an example of an internal structure of the test | inspection apparatus shown in FIG. It is a schematic diagram which shows 1st Embodiment of a micro test | inspection chip. It is a schematic diagram which shows the structure of the liquid injection port periphery in 1st Embodiment. It is a schematic diagram which shows the structure of the liquid injection port periphery in 2nd Embodiment. It is a schematic diagram which shows the conventional dividing method of the liquid.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Inspection apparatus 3 Tray 5 Conveyance port 7 Operation part 9 Display part 100 Micro test | inspection chip 101 Flow path board | substrate 103 Top plate 105 Flow path 107 (107a, 107b) Liquid supply flow path 111 Liquid storage part 113 Inlet 115 Divided flow path 117 Connection port 151 Liquid 300 Liquid injection port 301 Lid 303 Holding part 305 Hinge 307 Liquid reservoir part 309 (309a, 309b) Connection port 311 Protrusion part 313 Leakage receiver 315 Inner wall

  Hereinafter, the present invention will be described based on the illustrated embodiment, but the present invention is not limited to the embodiment. In the drawings, the same or equivalent parts are denoted by the same reference numerals, and redundant description is omitted.

  First, an inspection apparatus for performing an inspection using the micro inspection chip of the present invention will be described with reference to FIGS. FIG. 1 is a perspective view showing an example of an inspection apparatus 1.

  In FIG. 1, the inspection apparatus 1 includes a tray 3, a transport port 5, an operation unit 7, a display unit 9, and the like. A micro inspection chip 100 in which a specimen, a reagent, or the like is injected from a liquid injection port described later and the liquid injection port is sealed by a lid described later is set on the tray 3, and is inspected from the transport port 5 by a loading mechanism (not shown). It is conveyed into the apparatus 1 and inspected. Inspection contents, inspection target data, and the like are input to the inspection apparatus 1 using the operation unit 7, and inspection results are displayed on the display unit 9.

  FIG. 2 is a block diagram showing an example of the internal configuration of the inspection apparatus 1 shown in FIG.

  In FIG. 2, the inspection apparatus 1 includes a micro pump unit 210, a heating / cooling unit 230, a detection unit 250, a drive control unit 270, and the like. The micro inspection chip 100 is transported from the transport port 5 into the inspection apparatus 1 by a loading mechanism (not shown) and connected to the micro pump unit 210. Tests such as detection of a target substance and determination of disease are performed by mixing and reacting the specimen and reagent in the micro test chip 100 by liquid feeding by a micropump.

  The micro inspection chip 100 is equivalent to what is generally called an analysis chip, a microreactor chip, and the like. For example, the micro inspection chip 100 is made of resin, glass, silicon, ceramics, and the like. Is formed with a fine flow path having a level of several μm to several hundred μm. The size and shape of the micro inspection chip 100 is usually a plate shape having a length and width of several tens of mm and a thickness of several mm.

  Here, the micro test chip 100 is formed of a water-repellent resin material such as polypropylene, for example, and has a channel substrate 101 on the surface of which a groove-shaped channel for flowing a liquid such as a reagent or a specimen is formed. Suppose that it is composed of a top plate 103 that is bonded to the surface of the flow path substrate 101 on which the flow path is formed and functions as a groove-shaped flow path cover of the flow path substrate 101. The flow path substrate 101 and the top plate 103 are provided with a connection port between the micro pump unit 210 and the micro test chip 100 and a liquid injection port described later for injecting a specimen, a reagent, and the like.

  The micro pump unit 210 is a pump unit for performing liquid feeding in the micro test chip 100, and includes a micro pump 211, a chip connection unit 213, a driving liquid tank 215, a driving liquid supply unit 217, and the like. The micropump unit 210 includes one or a plurality of micropumps 211. The micropump 211 feeds the liquid in the micro test chip 100 by injecting or sucking the driving liquid 216 into the micro test chip 100. The chip connection unit 213 connects the micropump 211 and the micro inspection chip 100.

  The driving liquid supply unit 217 supplies the driving liquid 216 from the driving liquid tank 215 to the micropump 211. The driving liquid tank 215 can be removed and replaced from the driving liquid supply unit 217 to replenish the driving liquid 216. One or a plurality of pumps are formed on the micropump 211, and the plurality of pumps can be driven independently or in conjunction with each other.

  The micro inspection chip 100 and the micropump 211 are connected by a chip connection part 213 and communicated with each other. When the micro pump 211 is driven, the driving liquid 216 is injected or sucked into the micro test chip 100 from the micro pump 211 via the chip connection portion 213. Samples, reagents, and the like housed in a plurality of housing parts in the micro test chip 100 are sent in the micro test chip 100 by the driving liquid 216.

  The heating / cooling unit 230 includes a cooling unit 231, a heating unit 233, and the like, and heats and cools a specimen, a reagent, a mixed solution thereof, and the like in order to promote and suppress a reaction in the micro test chip 100. The cooling unit 231 includes a Peltier element or the like. The heating unit 233 includes a heater or the like. Of course, the heating unit 233 may also be formed of a Peltier element.

  The detection unit 250 includes a light source 251 such as a light emitting diode (LED) or a laser, a light receiving element 253 such as a photodiode (PD), and the like, and a target substance contained in a generated liquid obtained by a reaction in the micro inspection chip 100. Is detected optically at the position of the detection region 255 on the micro inspection chip 100.

  The drive control unit 270 includes a microcomputer, a memory, and the like (not shown), and drives, controls, and detects each unit in the inspection apparatus 1.

  Next, a first embodiment of the micro test chip according to the present invention will be described with reference to FIGS. 3A and 3B are schematic views showing the first embodiment of the micro inspection chip 100 according to the present invention. FIG. 3A is a plan view seen from the flow path substrate 101 side, and FIG. The side view seen from the arrow A side of a), FIG.3 (c) is the top view seen from the top-plate 103 side. Here, an example in which the liquid inlet 300 is provided on the flow path substrate 101 side is shown.

  In FIG. 3A, a holding part 303 constituting the liquid inlet 300 is provided on the flow path substrate 101. Further, a flow path 105 shown by a broken line is provided on the top plate 103 side of the flow path substrate 101. A connection port 309 for dividing the liquid injected into the liquid injection port 300 is provided inside the holding unit 303, and a liquid supply flow path for supplying the divided liquid immediately below the liquid injection port 300. 107 is provided.

  3B and 3C, a holding unit 303 and a lid 301 that seals the liquid inlet 300 by being inserted into the holding unit 303 are provided on the flow path substrate 101. If the holding unit 303 and the lid 301 are connected by, for example, a flexible hinge 305 or the like, there is no risk of the lid 301 being dropped or lost, which is convenient. As such a configuration, for example, in FIG. 5 and FIG. 6 of Japanese Patent Publication No. 9-504608, a blood sample is introduced from an opening using a sealable lid attached to a flexible hinge in the manner of snap fastening. After that, how to seal it is shown.

  On the top plate 103 side of the flow path substrate 101, a flow path 105 illustrated by a broken line is provided. A connection port 309 for dividing the liquid injected into the liquid injection port 300 is provided inside the holding unit 303, and a liquid supply flow path for supplying the divided liquid immediately below the liquid injection port 300. 107 is provided. The holding unit 303 and the liquid supply channel 107 are communicated with each other through a connection port 309. The top plate 103 is provided with a connection port 117 that connects the flow path 105 and the micropump.

  FIG. 4 is a schematic diagram showing a configuration around the liquid injection port 300 in the first embodiment of the micro test chip 100. FIG. 4A is a cross-sectional view taken along the line BB ′ of FIG. FIG. 5B is a plan view of the periphery of the liquid inlet 300 viewed from the flow path substrate 101 side. Here, an example in which the liquid is divided into two parts is shown, but the number of divisions is arbitrary and may be determined as necessary.

  4A and 4B, the liquid inlet 300 temporarily stores a lid 301 that seals the liquid inlet 300, a holding portion 303 that holds the lid 301 in a sealed state, and a liquid 151. It is comprised by the liquid reservoir part 307 grade | etc.,.

  The lid 301 and the holding portion 303 have a so-called snap fastening shape in which the lid 301 is inserted into the holding portion 303 and the claw portion 301a of the lid 301 is hooked on the groove portion 303a of the holding portion 303 and locked. Of course, other locking methods may be used.

  The holding part 303 has a liquid reservoir part 307 which is a mortar-like concave part inside. A plurality of connection ports 309 (here, two connection ports 309a and 309b) for dividing the liquid injected into the liquid storage portion 307 into a plurality of portions are provided in the middle of the mortar-shaped slope of the liquid storage portion 307. ing. The plurality of connection ports 309 are in communication with a plurality of liquid supply channels 107 (here, two liquid supply channels 107a and 107b) for supplying the liquid 151, respectively. The number of the connection ports 309 and the liquid supply channels 107 may be determined according to the required number of divisions for how many portions of the liquid 151 injected into the liquid reservoir 307 need to be divided.

  The lid 301 is provided with a protrusion 311 having a shape in which at least a part of the liquid reservoir 307 is inverted in shape, and when the lid 301 is inserted into the holding portion 303, the surface of the protrusion 311 collects the liquid. The part 307 is in close contact with all or part of the surface. The protrusion 311 and the liquid reservoir 307 may have exactly the same shape, but in this example, the portion of the tip 311a of the protrusion 311 that contacts the bottom of the liquid reservoir 307 is removed. That is, the tip 311 a of the protrusion 311 has a shape that does not contact the bottom of the liquid reservoir 307.

  Next, a method for dividing the liquid 151 will be described. First, the liquid 151 is dropped into the liquid reservoir 307 by a worker using a pipette or the like (dropping step). Subsequently, when the lid 301 is pushed down in the direction of arrow Z in the drawing and inserted into the holding portion 303 (insertion step), the liquid 151 is sandwiched between the liquid reservoir portion 307 and the projection portion 311 and the connection ports 309a and 309b. Are pushed out to the position of, and flow out to the liquid feeding passages 107a and 107b through the connection ports 309a and 309b (dividing step). Therefore, the liquid 151 can be easily and accurately divided into the required number of divisions (here, two divisions) simply by inserting the lid 301 into the holding portion 303. However, it is not essential to push out the liquid 151 by the protrusion 311.

  Further, when the lid 301 is inserted into the holding portion 303 and the claw portion 301a of the lid 301 is hooked and locked in the groove portion 303a of the holding portion 303, the surface of the liquid reservoir portion 307 and the surface of the protruding portion 311 are brought together. In order to be in close contact with each other, the connection ports 309a and 309b are completely separated, and the communication between the liquid supply channels 107a and 107b is completely separated (separation step). Thereby, the force of the micropump at the time of liquid 151 feeding does not affect each other and the liquid feeding becomes unstable, and stable liquid feeding can be performed.

  As described above, according to the first embodiment of the micro test chip 100, the liquid reservoir 307 provided inside the holding unit 303 of the liquid injection port 300 is matched with the required number of divisions of the liquid 151. A plurality of connection ports 309 and a plurality of liquid supply passages 107 are provided, and a protrusion 311 having a shape obtained by reversing the shape of at least a part of the liquid reservoir 307 on the lid 301 is provided. Thus, just by inserting the lid 301 into the holding portion 303, the surface of the projection 311 is in close contact with all or part of the surface of the liquid reservoir 307, and the liquid 151 is easily and accurately divided into the required number of divisions. be able to. Furthermore, since the communication between the plurality of liquid supply channels 107 can be completely separated, stable liquid supply can be provided.

  Next, a second embodiment of the micro test chip according to the present invention will be described with reference to FIG. FIG. 5 is a schematic diagram showing a configuration around the liquid injection port 300 in the second embodiment of the micro test chip 100, and FIG. 5A is a cross-sectional view taken along the line CC ′ of FIG. FIG. 5B is a plan view of the periphery of the liquid inlet 300 viewed from the top plate 103 side. Here, an example in which the liquid is divided into two parts is shown, but the number of divisions is arbitrary and may be determined as necessary. Here, an example in which the liquid inlet 300 is provided on the top plate 103 side is shown.

  5A and 5B, the liquid inlet 300 includes a lid 301 that seals the liquid inlet 300, a holding portion 303 that holds the lid 301 in a sealed state, and a liquid that temporarily stores the liquid 151. The reservoir 307, the leak receiver 313 that receives the liquid 151 overflowing from the reservoir 307, the inner wall 315 that separates the leak receiver 313 and the reservoir 307, and the like. The liquid inlet 300 is provided on the top plate 103 side of the flow path substrate 101 and protrudes from the opening 103 a of the top plate 103.

  The lid 301 and the holding portion 303 have a so-called screw-in shape in which a male screw provided on the inner wall of the lid 301 and a female screw provided on the outer periphery of the holding portion 303 are engaged and locked. Of course, other locking methods may be used.

  The holding part 303 has a liquid leakage receiver 313 that receives the liquid 151 overflowing from the liquid reservoir 307 inside, and further has a liquid reservoir part 307 that is a cylindrical recess with an inner wall 315 interposed therebetween. is doing. On the bottom surface of the liquid reservoir 307, a plurality of liquid supply channels 107 (here, two liquid supply channels 107a and 107b) for dividing and supplying the liquid injected into the liquid reservoir 307 into a plurality of parts. A part of each of the liquid supply channels 107a and 107b (the hatched portion in FIG. 5B) is opened at the bottom surface of the liquid reservoir 307. The number of the liquid supply channels 107 may be determined according to the required number of divisions for how many portions of the liquid 151 injected into the liquid reservoir 307 need to be divided.

  The lid 301 is provided with a protrusion 311 having a shape in which at least a part of the liquid reservoir 307 is inverted in shape. When the lid 301 is screwed into the holding portion 303, the surface of the protrusion 311 becomes the liquid reservoir. 307 is in close contact with all or part of the surface. The protrusion 311 and the liquid reservoir 307 may have the same shape as in this example, or may be partially different. However, it is desirable that the shape of the part of the bottom surface of the liquid reservoir 307 at the tip 311 a of the protrusion 311 that is in contact with the liquid feeding flow path 107 is the same as the bottom surface of the liquid reservoir 307.

  Next, a method for dividing the liquid 151 will be described. First, the liquid 151 is dropped into the liquid reservoir 307 by a worker using a pipette or the like (dropping step). Subsequently, when the lid 301 is pushed down in the direction of arrow Z in the figure and screwed into the holding portion 303 (insertion process), the liquid 151 is pushed out by being sandwiched between the liquid reservoir portion 307 and the projection portion 311. The liquid flows through the openings of the liquid supply channels 107a and 107b provided on the bottom surface of 307 and flows out into the liquid supply channels 107a and 107b (division step). Therefore, the liquid 151 can be easily and accurately divided into the required number of divisions (here, two divisions) simply by screwing the lid 301 into the holding portion 303. However, it is not essential to push out the liquid 151 by the protrusion 311.

  Further, when the lid 301 is screwed into the holding portion 303, the surface of the liquid reservoir 307 and the surface of the protrusion 311 are in close contact with each other, so that the communication between the liquid supply channels 107a and 107b is completely separated (separation). Process). Thereby, the force of the micropump at the time of liquid 151 feeding does not affect each other and the liquid feeding becomes unstable, and stable liquid feeding can be performed.

  The liquid reservoir 307 and the wall surfaces of the liquid supply channels 107a and 107b are used to smoothly flow the liquid 151 from the liquid reservoir 307 into the liquid supply channels 107a and 107b when the lid 301 is screwed into the holding unit 303. In addition, it is desirable to be hydrophilic. The hydrophilic level varies depending on the dimensions and shape of the flow path, but as a guide, the contact angle between the wall surface and pure water is preferably 50 ° or less, more preferably 30 ° or less.

  When the liquid reservoir 307 and the wall surfaces of the liquid supply channels 107a and 107b are hydrophilic, the dropped sample 151 spontaneously flows into the liquid supply channels 107a and 107b. In this case, the liquid 151 does not need to be pushed out by the protrusion 311 of the lid 301, and the protrusion 311 plays a role of separating the liquid supply channels 107a and 107b.

  Since the liquid 151 overflowing from the liquid reservoir 307 when the lid 301 is screwed into the holding part 303 flows into the leak receiver 313, even if the liquid 151 is a specimen containing, for example, a dangerous virus or the like, There is no leakage to the outside, and safety is maintained.

  As described above, according to the second embodiment of the micro inspection chip 100, the required number of divisions of the liquid 151 is provided below the liquid reservoir 307 provided inside the holding unit 303 of the liquid inlet 300. In addition, a plurality of liquid supply passages 107 are provided and opened at the bottom of the liquid reservoir 307, and a protrusion 311 having a shape obtained by reversing the shape of at least a part of the liquid reservoir 307 on the lid 301 is provided. Thus, the surface of the protrusion 311 is in close contact with all or part of the surface of the liquid reservoir 307 simply by screwing the lid 301 into the holding portion 303, and the liquid 151 is easily and accurately divided into the necessary number of divisions. Can do. Furthermore, since the communication between the plurality of liquid supply channels 107 can be completely separated, stable liquid supply can be provided.

  As described above, according to the present invention, the liquid stored in the liquid reservoir provided in the liquid inlet is supplied to the plurality of connection ports provided in the liquid reservoir or directly. The liquid can be divided into a plurality of parts by pouring into the liquid feed flow path. Further, by sealing the plurality of connection ports with the protrusions provided on the lid that seals the liquid injection port, communication between the plurality of liquid supply channels can be prevented. Thereby, a micro inspection chip and a microchip that can divide and inject a small amount of liquid into a plurality of liquid supply flow paths with a simple operation with high accuracy and can stably supply liquid after divided injection. The liquid dividing method can be provided.

  The detailed configuration and detailed operation of each component constituting the micro inspection chip and the microchip liquid dividing method according to the present invention can be changed as appropriate without departing from the spirit of the present invention.

Claims (6)

In a micro inspection chip comprising a liquid inlet for injecting liquid and a plurality of liquid supply passages for dividing and supplying the injected liquid,
The liquid inlet has a lid that seals the liquid inlet, a holding portion that holds the lid, and a liquid reservoir that temporarily stores liquid,
The liquid reservoir has a plurality of connection ports communicated with each of the plurality of liquid supply passages,
The micro inspection chip according to claim 1, wherein the lid has a protrusion that prevents communication between the plurality of liquid supply channels when the lid is inserted into the holding portion. The protruding portion has a shape that is in close contact with all or a part of the liquid reservoir when the lid is inserted into the holding portion.
By inserting the lid into the holding part,
2. The liquid according to claim 1, wherein the protrusion drops the liquid dropped into the liquid reservoir into the plurality of connection ports, and divides the liquid into a plurality of flow paths. Micro inspection chip. In a micro inspection chip comprising a liquid inlet for injecting liquid and a plurality of liquid supply passages for dividing and supplying the injected liquid,
The liquid inlet has a lid that seals the liquid inlet, a holding portion that holds the lid, and a liquid reservoir that temporarily stores liquid,
The plurality of liquid supply passages are disposed at the lower part of the liquid reservoir, and a part or all of each is open on the bottom surface of the liquid reservoir,
The micro inspection chip according to claim 1, wherein the lid has a protrusion that prevents communication between the plurality of liquid supply channels when the lid is inserted into the holding portion. The protruding portion has a shape that is in close contact with all or a part of the liquid reservoir when the lid is inserted into the holding portion.
By inserting the lid into the holding part,
The micro test chip according to claim 3, wherein the liquid dropped into the liquid reservoir is divided into a plurality of parts and poured into the plurality of liquid supply channels by the protrusions.   The micro test chip according to claim 3 or 4, wherein the liquid reservoir and the wall surfaces of the plurality of liquid supply channels have hydrophilicity. In the liquid dividing method using the micro inspection chip according to any one of claims 1 to 5,
A dropping step of dropping a liquid into the liquid reservoir;
An insertion step of inserting the lid into the holding portion;
A dividing step of dividing the liquid into the plurality of liquid feeding flow paths by the protrusions;
A liquid dividing method for a micro test chip, comprising: a separation step of sealing a plurality of the liquid supply flow paths to prevent communication between the plurality of liquid supply flow paths.