JPWO2009060695A1 - Microchip inspection device - Google Patents
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- JPWO2009060695A1 JPWO2009060695A1 JP2009539997A JP2009539997A JPWO2009060695A1 JP WO2009060695 A1 JPWO2009060695 A1 JP WO2009060695A1 JP 2009539997 A JP2009539997 A JP 2009539997A JP 2009539997 A JP2009539997 A JP 2009539997A JP WO2009060695 A1 JPWO2009060695 A1 JP WO2009060695A1
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- 238000007689 inspection Methods 0.000 title claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 98
- 239000000463 material Substances 0.000 claims abstract description 24
- 238000010521 absorption reaction Methods 0.000 claims abstract description 19
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 41
- 239000001301 oxygen Substances 0.000 claims description 41
- 229910052760 oxygen Inorganic materials 0.000 claims description 41
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 19
- 239000002250 absorbent Substances 0.000 claims description 17
- 230000002745 absorbent Effects 0.000 claims description 17
- 239000007789 gas Substances 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 11
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 6
- 229910052742 iron Inorganic materials 0.000 claims description 5
- 239000000565 sealant Substances 0.000 claims description 5
- 239000011358 absorbing material Substances 0.000 claims description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 235000010265 sodium sulphite Nutrition 0.000 claims description 3
- 239000012530 fluid Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- -1 polyethylene terephthalate Polymers 0.000 description 5
- 239000004698 Polyethylene Substances 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 4
- 239000006096 absorbing agent Substances 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
- G01N2035/1018—Detecting inhomogeneities, e.g. foam, bubbles, clots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
- G01N2035/1039—Micropipettes, e.g. microcapillary tubes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
ポンプ内での気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得るために、マイクロチップ収容部と、マイクロチップの被検出部に対応して設けられた検出部と、マイクロチップの流路に駆動液を注入するマイクロポンプと、マイクロポンプに供給する駆動液を収容する駆動液タンクと、を有し、マイクロポンプより上流の駆動液の流路の一部が、気体吸収機能を有する素材で形成されているマイクロチップ検査装置とする。In order to obtain a microchip inspection apparatus that solves the problem of liquid feeding caused by bubbles in the pump, a microchip housing section, a detection section provided corresponding to the detected section of the microchip, A micropump for injecting the driving liquid into the flow path and a driving liquid tank for storing the driving liquid to be supplied to the micropump, and a part of the flow path of the driving liquid upstream from the micropump has a gas absorption function The microchip inspection apparatus is made of a material having the same.
Description
本発明は、マイクロチップ検査装置に関するものである。 The present invention relates to a microchip inspection apparatus.
近年、マイクロマシン技術および超微細加工技術を駆使することにより、従来の試料調製、化学分析、化学合成などを行うための装置、手段(例えばポンプ、バルブ、流路、センサーなど)を微細化して1チップ上に集積化したシステムが注目されている。これは、μ−TAS(Micro Total Analysis System)とも呼ばれ、マイクロチップといわれる部材に、試薬と試料(例えば、検査を受ける被験者の尿、唾液、血液を処理して抽出したDNA抽出溶液など)を合流させ、その反応を検出することにより試料の特性を調べるものである。 In recent years, by making full use of micromachine technology and ultrafine processing technology, devices and means (for example, pumps, valves, flow paths, sensors, etc.) for performing conventional sample preparation, chemical analysis, chemical synthesis, etc. have been miniaturized. A system integrated on a chip attracts attention. This is also called μ-TAS (Micro Total Analysis System), and a reagent and a sample (for example, a DNA extraction solution obtained by processing urine, saliva, blood, etc. of a subject under examination) on a member called a microchip. The characteristics of the sample are examined by detecting the reaction.
マイクロチップは、樹脂材料やガラス材料からなる基体に、試薬や試料を流すことができる微細な流路と試薬を蓄える液溜部を設けており、さまざまなパターンが提案されている。 In the microchip, a substrate made of a resin material or a glass material is provided with a fine channel through which a reagent or sample can flow and a liquid reservoir for storing the reagent, and various patterns have been proposed.
そして、これらマイクロチップを用いて試料の特性を調べる際は、マイクロポンプなどで駆動液をマイクロチップの微細流路に送液し、マイクロチップ内に収容されている試薬や試料を押し出すことにより、試薬と試料とを反応させて被検出部に導き、検出を行う。被検出部では、例えば光学的な検出方法などによって目的物質の検出が行われる。 And when examining the characteristics of the sample using these microchips, the driving liquid is sent to the microchannel of the microchip with a micropump, etc., and the reagent or sample contained in the microchip is pushed out, The reagent and the sample are reacted and guided to the detected part to perform detection. In the detected portion, the target substance is detected by, for example, an optical detection method.
このようなマイクロチップ検査装置として、例えば以下の特許文献1に記載のような検査装置が開示されている。
各種の分析、検査では、これらのマイクロチップにおける分析の定量性、解析の精度、経済性などが重要視される。そのためには、シンプルな構成で、高い信頼性の送液システムを確立することが望まれている。 In various types of analysis and inspection, importance is attached to the quantitativeness of analysis, the accuracy of analysis, and the economy of these microchips. For this purpose, it is desired to establish a highly reliable liquid feeding system with a simple configuration.
しかしながら、マイクロチップの微細流路に駆動液を送液するポンプ内で気泡が発生すると、マイクロポンプが正確な送液を行うことができなくなる問題がある。 However, if bubbles are generated in the pump that feeds the driving liquid to the microchannel of the microchip, there is a problem that the micropump cannot perform accurate liquid feeding.
本発明は上記問題に鑑み、ポンプ内での気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得ることを目的とするものである。 In view of the above problems, an object of the present invention is to obtain a microchip inspection apparatus that solves the problem of liquid feeding caused by bubbles in a pump.
上記の目的は、下記構成により解決される。 The above object is solved by the following configuration.
1.試料の混合、反応、検出が行われる流路の形成されたマイクロチップを収容するマイクロチップ収容部と、該マイクロチップ収容部に収容されたマイクロチップの被検出部に対応して設けられた検出部と、前記マイクロチップの流路に駆動液を注入するマイクロポンプと、該マイクロポンプに供給する駆動液を収容する駆動液タンクと、を有し、前記マイクロポンプより上流の前記駆動液の流路の一部が、気体吸収機能を有する素材で形成されていることを特徴とするマイクロチップ検査装置。 1. A microchip housing part that houses a microchip in which a channel for mixing, reacting, and detecting a sample is formed, and a detection provided corresponding to the detected part of the microchip housed in the microchip housing part And a micropump for injecting the driving liquid into the flow path of the microchip, and a driving liquid tank for storing the driving liquid supplied to the micropump, and the flow of the driving liquid upstream of the micropump. A microchip inspection apparatus, wherein a part of the path is formed of a material having a gas absorption function.
2.前記気体吸収機能を有する素材に吸収される気体は、酸素であることを特徴とする前記1.に記載のマイクロチップ検査装置。 2. The gas that is absorbed by the material having the gas absorbing function is oxygen. The microchip inspection apparatus according to 1.
3.前記気体吸収機能を有する素材は、鉄系の酸素吸収素材であることを特徴とする前記1.又は2.に記載のマイクロチップ検査装置。 3. The material having the gas absorbing function is an iron-based oxygen absorbing material. Or 2. The microchip inspection apparatus according to 1.
4.前記酸素吸収素材を加熱する加熱手段を有することを特徴とする前記3.に記載のマイクロチップ検査装置。 4). 2. A heating means for heating the oxygen absorbing material. The microchip inspection apparatus according to 1.
5.前記駆動液タンクが前記気体吸収機能を有する素材を用いて形成されていることを特徴とする前記1.乃至4.のいずれかに記載のマイクロチップ検査装置。 5. The driving liquid tank is formed using a material having the gas absorption function. To 4. The microchip test | inspection apparatus in any one of.
6.前記マイクロポンプと前記駆動液タンクの間に中間袋を有し、前記中間袋が前記気体吸収機能を有する素材を用いて形成されていることを特徴とする前記1.乃至4.のいずれかに記載のマイクロチップ検査装置。 6). An intermediate bag is provided between the micro pump and the driving liquid tank, and the intermediate bag is formed using a material having the gas absorption function. To 4. The microchip test | inspection apparatus in any one of.
7.前記気体吸収機能を有する素材は、少なくともアルミニウム層、酸素吸収層、シーラント層を有するフィルム状のものであることを特徴とする前記5.又は6.に記載のマイクロチップ検査装置。 7. 4. The material having a gas absorbing function is a film having at least an aluminum layer, an oxygen absorbing layer, and a sealant layer. Or 6. The microchip inspection apparatus according to 1.
8.試料の混合、反応、検出が行われる流路の形成されたマイクロチップを収容するマイクロチップ収容部と、該マイクロチップ収容部に収容されたマイクロチップの被検出部に対応して設けられた検出部と、前記マイクロチップの流路に駆動液を注入するマイクロポンプと、該マイクロポンプに供給する駆動液を収容する駆動液タンクと、を有し、前記マイクロポンプより上流に酸素吸収剤添加部を配置したことを特徴とするマイクロチップ検査装置。 8). A microchip housing part that houses a microchip in which a channel for mixing, reacting, and detecting a sample is formed, and a detection provided corresponding to the detected part of the microchip housed in the microchip housing part And a micropump for injecting the driving liquid into the flow path of the microchip, and a driving liquid tank for storing the driving liquid supplied to the micropump, and an oxygen absorbent addition unit upstream of the micropump A microchip inspection apparatus characterized by comprising:
9.前記酸素吸収剤添加部により添加される酸素吸収剤は、亜硫酸ナトリウムであることを特徴とする前記8.に記載のマイクロチップ検査装置。 9. 8. The oxygen absorbent added by the oxygen absorbent addition section is sodium sulfite. The microchip inspection apparatus according to 1.
本発明によれば、ポンプ内の気泡の発生を抑制でき、気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得ることができる。 ADVANTAGE OF THE INVENTION According to this invention, generation | occurrence | production of the bubble in a pump can be suppressed and the microchip test | inspection apparatus which eliminated the malfunction of the liquid feeding resulting from a bubble can be obtained.
1 マイクロチップ検査装置
2 ポンプカートリッジ
3 マイクロチップ
4 マイクロチップ挿入口
5 駆動液タンク
6 タンク装着台
7 ジョイント部
8 電磁弁
9 中間袋
10 フィルター部
11 マイクロポンプ
12 中間流路部材
13 ベース基材
14 中継基板
60 接続部
61 酸素吸収剤添加部
C1 第1ジョイント部
C2 第2ジョイント部
C3 コネクタ部
D1 電源部
T1 チューブDESCRIPTION OF SYMBOLS 1 Microchip test | inspection apparatus 2 Pump cartridge 3 Microchip 4 Microchip insertion port 5 Drive liquid tank 6 Tank mounting stand 7 Joint part 8 Electromagnetic valve 9 Intermediate bag 10 Filter part 11 Micropump 12 Intermediate flow path member 13 Base base material 14 Relay Substrate 60 Connection portion 61 Oxygen absorbent addition portion C1 First joint portion C2 Second joint portion C3 Connector portion D1 Power supply portion T1 Tube
以下、実施の形態により本発明を詳しく説明するが、本発明はこれに限定されるものではない。 Hereinafter, the present invention will be described in detail with reference to embodiments, but the present invention is not limited thereto.
(第1の実施の形態)
図1は、第1の実施の形態に係るマイクロチップ検査装置の一例を示す断面図である。(First embodiment)
FIG. 1 is a cross-sectional view showing an example of a microchip inspection apparatus according to the first embodiment.
同図において、1はマイクロチップ検査装置本体であり、マイクロチップ3が装置本体1のマイクロチップ挿入口4から、矢印Aの方向に着脱可能になっている。さらに、ポンプカートリッジ2は、装置本体1の奥側上部に装着されるように構成され、装置本体1とポンプカートリッジ2は第1ジョイント部C1によって液の漏洩がないように接続されている。また装置本体1は、装置の各部に電力を供給するための電源部D1を有しており、ポンプカートリッジ2は、装置本体1に装着した際に電源部D1と電気的に接続されるコネクタ部C3を介して電源部D1から電力が供給される。 In the figure, reference numeral 1 denotes a microchip inspection apparatus main body, and the microchip 3 is detachable from the microchip insertion port 4 of the apparatus main body 1 in the direction of arrow A. Further, the pump cartridge 2 is configured to be mounted on the upper back side of the apparatus main body 1, and the apparatus main body 1 and the pump cartridge 2 are connected by the first joint portion C <b> 1 so that no liquid leaks. Further, the apparatus main body 1 has a power supply part D1 for supplying power to each part of the apparatus, and the pump cartridge 2 is a connector part electrically connected to the power supply part D1 when mounted on the apparatus main body 1. Power is supplied from the power supply unit D1 via C3.
また、マイクロチップ3内の検体と試薬との反応状態等を検出するための、例えば光発光部41と光受光部42等からなる検出部40を備えている。 In addition, a detection unit 40 including, for example, a light emitting unit 41, a light receiving unit 42, and the like for detecting a reaction state between the sample and the reagent in the microchip 3 is provided.
(ポンプカートリッジ)
次にポンプカートリッジ2の詳細について説明する。(Pump cartridge)
Next, details of the pump cartridge 2 will be described.
ポンプカートリッジ2には、駆動液を収容する駆動液タンク5と、駆動液タンク5を載せるタンク装着台6と、駆動液タンク5と接続して駆動液を後工程に送液するためのジョイント部7を有している。また、送液された液を制御する電磁弁8、送液された液を一旦貯蔵する伸縮可能な中間袋9、液内にある不純物を取り除くフィルター部10、駆動液を送液する液駆動用ポンプ11(マイクロポンプ11という)、駆動液をマイクロチップ3に送液するための中間流路部材12がある。さらに、マイクロポンプ11を取り付けるベース基材13と、コネクターC3から供給される電気をポンプカートリッジ2の各部に供給する中継基板14を主な構成としている。ポンプカートリッジ2は、駆動液タンク5からマイクロポンプ11を経由して中間流路部材12に至るまで流路内に駆動液が予め満たされており、この状態で交換が行われるようになっている。 The pump cartridge 2 includes a driving liquid tank 5 that contains the driving liquid, a tank mounting base 6 on which the driving liquid tank 5 is placed, and a joint part that is connected to the driving liquid tank 5 and sends the driving liquid to a subsequent process. 7. In addition, an electromagnetic valve 8 for controlling the supplied liquid, an extendable intermediate bag 9 for temporarily storing the supplied liquid, a filter unit 10 for removing impurities in the liquid, and a liquid drive for supplying driving liquid There is a pump 11 (referred to as micropump 11), and an intermediate flow path member 12 for sending drive liquid to the microchip 3. Furthermore, the base substrate 13 to which the micropump 11 is attached and the relay substrate 14 that supplies electricity supplied from the connector C3 to each part of the pump cartridge 2 are mainly configured. In the pump cartridge 2, the driving liquid is filled in advance from the driving liquid tank 5 to the intermediate flow path member 12 via the micropump 11, and replacement is performed in this state. .
本実施の形態では、駆動液タンク5をポンプカートリッジ2の上部に配置して、駆動液タンク5から送液される駆動液が、重力の作用により流れ出るようにしてある。そして、駆動液タンク5を交換するときは、蓋Fを開いて矢印Bの方向に着脱することによって行うことができる。さらに駆動液タンク5は、図1に示すように傾けて載置され、駆動液タンク5の液出口が最も低い位置になるようしている。 In the present embodiment, the driving liquid tank 5 is arranged above the pump cartridge 2 so that the driving liquid fed from the driving liquid tank 5 flows out by the action of gravity. Then, the drive fluid tank 5 can be replaced by opening the lid F and attaching / detaching it in the direction of arrow B. Further, the driving liquid tank 5 is placed inclined as shown in FIG. 1 so that the liquid outlet of the driving liquid tank 5 is at the lowest position.
また、駆動液タンク5を着脱可能とするために、本実施の形態では駆動液タンク5側にメスジョイント、タンク装着台6側にオスジョイントを設けている。オス、メス一対のジョイントは、抜脱する際は駆動液が漏洩しない構成としたジョイントを使用している。また駆動液の粘性が1〜10mPa・s(環境温度25℃のとき)の範囲のものであれば、駆動液タンク5側の液出口部にゴムなどの弾性部材を設け、タンク装着台6側に例えば注射針のような細い針を設けて、着脱可能なようにしてもよい(参考特許文献特開2005−11187号公報)。 In order to make the drive fluid tank 5 detachable, in the present embodiment, a female joint is provided on the drive fluid tank 5 side and a male joint is provided on the tank mounting base 6 side. The pair of male and female joints uses a joint that is configured such that the driving fluid does not leak when being removed. Further, if the viscosity of the driving liquid is in the range of 1 to 10 mPa · s (when the ambient temperature is 25 ° C.), an elastic member such as rubber is provided at the liquid outlet on the driving liquid tank 5 side, and the tank mounting base 6 side For example, a thin needle such as an injection needle may be provided so as to be detachable (see Japanese Patent Application Laid-Open No. 2005-11187).
電磁弁8は、駆動液タンク5とマイクロポンプ11を繋ぐ流路の途中で駆動液タンク5より低く、マイクロポンプ11より高い位置に配置されている。また、伸縮可能な中間袋9の近傍には、その液量を検知する液量検知センサS1が設けられ、中間袋9に駆動液が送液されると共に袋が膨らんでくる位置を検知する。電磁弁8は、液量検知センサS1のON/OFF信号により弁の開閉を行い、チューブから送液される駆動液の通過量をON/OFF制御する。 The electromagnetic valve 8 is disposed at a position lower than the driving liquid tank 5 and higher than the micro pump 11 in the middle of the flow path connecting the driving liquid tank 5 and the micro pump 11. Further, a liquid amount detection sensor S1 for detecting the liquid amount is provided in the vicinity of the extendable intermediate bag 9, and detects the position where the driving liquid is supplied to the intermediate bag 9 and the bag is inflated. The electromagnetic valve 8 opens and closes the valve according to the ON / OFF signal of the liquid amount detection sensor S1, and performs ON / OFF control of the passing amount of the driving liquid fed from the tube.
また、中間袋9内の水位位置h1と、マイクロチップ3の駆動液流入口部の位置h2との差Δh=(h1−h2)は静水圧と呼ばれ、中間袋9の取付位置を上下に移動することによりマイクロチップ3に流入する駆動液を制御している。 Further, the difference Δh = (h1−h2) between the water level position h1 in the intermediate bag 9 and the position h2 of the driving liquid inlet of the microchip 3 is called hydrostatic pressure, and the mounting position of the intermediate bag 9 is set up and down. The driving liquid flowing into the microchip 3 is controlled by moving.
なお、駆動液タンク5内の駆動液の残量を検知するために、タンク装着台6に液残量検知センサS2を設けている。液残量検知センサS2は、重さを信号情報として発信するロードセルなどが使われる。ロードセルからの信号情報は、装置本体1が有する制御部に一旦入力されその信号によって、図示しないが装置本体1の表示部に液残量を表示したり、あるいは警告ブザーを鳴らして、駆動液タンク内の駆動液の残量をユーザ(担当者)に知らせる。 In order to detect the remaining amount of the driving liquid in the driving liquid tank 5, a liquid remaining amount detecting sensor S2 is provided on the tank mounting base 6. The liquid remaining amount detection sensor S2 uses a load cell that transmits weight as signal information. The signal information from the load cell is once input to the control unit of the apparatus main body 1 and, depending on the signal, the remaining amount of liquid is displayed on the display unit of the apparatus main body 1 or a warning buzzer is sounded. The user (person in charge) is informed of the remaining amount of the driving fluid inside.
駆動液タンク5や中間袋9は、例えば鉄粉系酸素吸収剤を使用した成形容器や、鉄粉系酸素吸収剤を使用したフィルムで容器を構成されていることが好ましい。 It is preferable that the driving liquid tank 5 and the intermediate bag 9 are made up of a container made of, for example, a molded container using an iron powder-based oxygen absorbent or a film using an iron powder-based oxygen absorbent.
図2は、鉄粉系酸素吸収剤を使用したフィルムの層構成の一例を示す断面模式図である。 FIG. 2 is a schematic cross-sectional view showing an example of a layer structure of a film using an iron powder-based oxygen absorbent.
同図に示すように、鉄粉系酸素吸収剤を使用したフィルム断面は、容器外側より、外装21、バリア層22、酸素吸収層23、シーラント層24で構成されている。外装21は、例えばナイロンやPET(ポリエチレンテレフタレート)等で形成されている。バリア層22は、例えばアルミ箔等で形成されている。酸素吸収層23は、例えばPP(ポリプロピレン)やPE(ポリエチレン)に鉄系酸素吸収剤を含有させて形成された層である。シーラント層24は、例えばPP(ポリプロピレン)やPE(ポリエチレン)で形成された層である。このような構成のフィルムで駆動液タンク5や中間袋9を形成することができる。 As shown in the figure, a film cross section using an iron powder-based oxygen absorbent is composed of an outer package 21, a barrier layer 22, an oxygen absorbing layer 23, and a sealant layer 24 from the outside of the container. The exterior 21 is made of, for example, nylon or PET (polyethylene terephthalate). The barrier layer 22 is made of, for example, aluminum foil. The oxygen absorption layer 23 is a layer formed by, for example, containing PP (polypropylene) or PE (polyethylene) with an iron-based oxygen absorbent. The sealant layer 24 is a layer formed of, for example, PP (polypropylene) or PE (polyethylene). The driving liquid tank 5 and the intermediate bag 9 can be formed with the film having such a configuration.
また、バリア層22にアルミ箔を用いることで、容器外部とのバリア性を大幅に向上させることができる。また、シーラント層24を有していることで簡易に、密閉や他素材との溶着が可能とすることができる。 Moreover, the barrier property with respect to the outside of the container can be greatly improved by using an aluminum foil for the barrier layer 22. Further, since the sealant layer 24 is provided, it can be easily sealed and welded to other materials.
また、駆動液と接触する壁面が鉄系の酸素吸収機能を有する素材で形成された容器や流路を用いる場合、該酸素吸収機能部近傍にある駆動液を加熱する加熱部を備えていることが好ましい。加熱部を設けることにより、該鉄系の酸素吸収機能を有する素材の酸素吸収速度を速めることができる。 In addition, when using a container or flow path whose wall surface in contact with the driving liquid is made of an iron-based material having an oxygen absorption function, a heating section for heating the driving liquid in the vicinity of the oxygen absorption function section is provided. Is preferred. By providing the heating unit, the oxygen absorption rate of the iron-based material having an oxygen absorption function can be increased.
図3は、鉄粉系酸素吸収剤を使用した容器で形成された駆動液タンク5、中間袋9を加熱する加熱部を有するマイクロチップ検査装置の断面図である。 FIG. 3 is a cross-sectional view of a microchip inspection apparatus having a driving liquid tank 5 formed of a container using an iron powder-based oxygen absorbent and a heating unit for heating the intermediate bag 9.
同図に示すように、駆動液タンク5の底面側にヒータ25、中間袋9の側面にヒータ29が配置されている。このヒータにより、例えば、駆動液を40℃以上に加熱することにより酸素吸収速度を速めることができる。 As shown in the figure, a heater 25 is disposed on the bottom surface side of the driving liquid tank 5, and a heater 29 is disposed on the side surface of the intermediate bag 9. With this heater, for example, the oxygen absorption rate can be increased by heating the driving liquid to 40 ° C. or higher.
なお、図3においては、駆動液タンク5と中間袋9が、鉄粉系酸素吸収剤を使用した容器で形成されている例を示したが、いずれか一方が鉄粉系酸素吸収剤を使用した容器で形成されているものでもよい。 In addition, in FIG. 3, although the drive fluid tank 5 and the intermediate bag 9 showed the example formed with the container using an iron powder type oxygen absorber, either one uses an iron powder type oxygen absorber. It may be formed of a container.
図1に戻り、マイクロポンプ11について説明する。 Returning to FIG. 1, the micropump 11 will be described.
マイクロポンプ11は、ポンプ室52、ポンプ室52の容積を変化させる圧電素子51、ポンプ室52の中間流路部材12側に位置する第1絞り流路53、ポンプ室52のフィルタ部10側に位置する第2絞り流路54、等から構成され、ポンプカートリッジ内に平置されている。第1絞り流路53及び第2絞り流路54は絞られた狭い流路となっており、また、第1絞り流路53は第2絞り流路54よりも長い流路となっている。 The micropump 11 includes a pump chamber 52, a piezoelectric element 51 that changes the volume of the pump chamber 52, a first throttle channel 53 positioned on the intermediate channel member 12 side of the pump chamber 52, and a filter unit 10 side of the pump chamber 52. The second throttle channel 54 and the like are positioned, and are placed flat in the pump cartridge. The first throttle channel 53 and the second throttle channel 54 are narrow and narrow channels, and the first throttle channel 53 is longer than the second throttle channel 54.
駆動液を順方向(中間流路部材12に向かう方向)に送液する場合には、まず、ポンプ室52の容積を急激に減少させるように圧電素子51を駆動する。そうすると、短い絞り流路である第2絞り流路54において乱流が発生し、第2絞り流路54における流路抵抗が長い絞り流路である第1絞り流路53に比べて相対的に大きくなる。これにより、ポンプ室52内の駆動液11は、第1絞り流路53の方に支配的に押し出され送液される。次に、ポンプ室52の容積を緩やかに増加させるように圧電素子51を駆動する。そうすると、ポンプ室52内の容積増加に伴って駆動液が第1絞り流路53及び第2絞り流路54から流れ込む。このとき、第2絞り流路54の方が第1絞り流路53と比べて長さが短いので、第2絞り流路54の方が第1絞り流路53と比べて流路抵抗が小さくなり、ポンプ室52内には第2絞り流路54の方から支配的に駆動液が流入する。以上の動作を圧電素子51が繰り返すことにより、駆動液が順方向に送液されることになる。 When the driving liquid is fed in the forward direction (the direction toward the intermediate flow path member 12), first, the piezoelectric element 51 is driven so as to rapidly reduce the volume of the pump chamber 52. Then, a turbulent flow is generated in the second throttle channel 54 that is a short throttle channel, and the channel resistance in the second throttle channel 54 is relatively larger than that of the first throttle channel 53 that is a throttle channel. growing. As a result, the driving liquid 11 in the pump chamber 52 is predominantly pushed toward the first throttle channel 53 and fed. Next, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is gradually increased. Then, the driving fluid flows from the first throttle channel 53 and the second throttle channel 54 as the volume in the pump chamber 52 increases. At this time, since the length of the second throttle channel 54 is shorter than that of the first throttle channel 53, the channel resistance of the second throttle channel 54 is smaller than that of the first throttle channel 53. Thus, the driving liquid flows into the pump chamber 52 predominantly from the second throttle channel 54. When the piezoelectric element 51 repeats the above operation, the driving liquid is fed in the forward direction.
一方、駆動液を逆方向(フィルタ部10に向かう方向)に送液する場合には、まず、ポンプ室52の容積を緩やかに減少させるように圧電素子51を駆動する。そうすると、第2絞り流路54の方が第1絞り流路53と比べて長さが短いので、第2絞り流路54の方が第1絞り流路53と比べて流路抵抗が小さくなる。これにより、ポンプ室52内の駆動液は、第2絞り流路54の方に支配的に押し出され送液される。次に、ポンプ室52の容積を急激に増加させるように圧電素子51を駆動する。そうすると、ポンプ室52内の容積増加に伴って駆動液が第1絞り流路53及び第2絞り流路54から流れ込む。このとき、短い絞り流路である第2絞り流路54において乱流が発生し、第2絞り流路54における流路抵抗が長い絞り流路である第1絞り流路53に比べて相対的に大きくなる。これにより、ポンプ室52内には第1絞り流路53の方から支配的に駆動液が流入する。以上の動作を圧電素子51が繰り返すことにより、駆動液が逆方向に送液されることになる。 On the other hand, when the driving liquid is fed in the reverse direction (the direction toward the filter unit 10), first, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is gradually reduced. Then, since the length of the second throttle channel 54 is shorter than that of the first throttle channel 53, the channel resistance of the second throttle channel 54 is smaller than that of the first throttle channel 53. . As a result, the driving liquid in the pump chamber 52 is predominantly pushed out toward the second throttle channel 54 and fed. Next, the piezoelectric element 51 is driven so that the volume of the pump chamber 52 is rapidly increased. Then, the driving fluid flows from the first throttle channel 53 and the second throttle channel 54 as the volume in the pump chamber 52 increases. At this time, turbulent flow is generated in the second throttle channel 54, which is a short throttle channel, and the channel resistance in the second throttle channel 54 is relatively larger than that of the first throttle channel 53, which is a throttle channel. Become bigger. As a result, the driving fluid flows into the pump chamber 52 predominantly from the first throttle channel 53. When the piezoelectric element 51 repeats the above operation, the driving liquid is fed in the reverse direction.
このように、マイクロポンプより上流の駆動液の流路の一部が、気体吸収機能を有する素材で形成されていることで、ポンプに送液する前に駆動液内に溶存する気体を吸収することができ、ポンプ内での気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得ることができる。 In this way, a part of the flow path of the driving liquid upstream from the micro pump is formed of a material having a gas absorption function, so that the gas dissolved in the driving liquid is absorbed before being sent to the pump. Therefore, a microchip inspection apparatus that can solve the problem of liquid feeding caused by bubbles in the pump can be obtained.
更に、駆動液が貯められる駆動液タンク5及び中間袋9の少なくとも一方を、酸素吸収機能を有する素材を用いて形成することで、駆動液と接する表面積を大きくすることができ、大きな酸素吸収効果を得ることができ、ポンプ内での気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得ることができる。 Furthermore, by forming at least one of the driving liquid tank 5 and the intermediate bag 9 in which the driving liquid is stored using a material having an oxygen absorption function, the surface area in contact with the driving liquid can be increased, and a large oxygen absorption effect can be obtained. And a microchip inspection apparatus that eliminates the problem of liquid feeding caused by bubbles in the pump.
なお、駆動液タンク5及び中間袋9の少なくとも一方が、酸素吸収機能を有する素材を用いて形成された例で説明したが、これに限るものでなく、駆動液が流れるチューブT1が酸素吸収機能を有する素材を用いて形成されたものであってもよい。 In addition, although at least one of the driving liquid tank 5 and the intermediate bag 9 has been described with an example formed using a material having an oxygen absorbing function, the present invention is not limited to this, and the tube T1 through which the driving liquid flows is an oxygen absorbing function. It may be formed using a material having
(第2の実施の形態)
図4は、第2の実施の形態に係るマイクロチップ検査装置を示す断面図である。同図は、図1に示すマイクロチップ検査装置と異なる部分についてのみ説明する。(Second Embodiment)
FIG. 4 is a cross-sectional view showing a microchip inspection apparatus according to the second embodiment. In the figure, only parts different from the microchip inspection apparatus shown in FIG. 1 will be described.
図4に示すマイクロチップ検査装置には、図示の如く、マイクロポンプ11より上流の、マイクロポンプ11とチューブT1との接続部60に、酸素吸収剤添加部61が配置されている。この酸素吸収剤添加部61は接続部60において、例えば、酸素吸収剤である亜硫酸ナトリウムを適宜添加し、駆動液中に溶存した酸素を除去するようになっている。 In the microchip inspection apparatus shown in FIG. 4, as shown in the figure, an oxygen absorbent addition unit 61 is arranged at a connection part 60 between the micropump 11 and the tube T1 upstream from the micropump 11. The oxygen absorbent addition section 61 is configured to add, for example, sodium sulfite as an oxygen absorbent as appropriate at the connection section 60 to remove oxygen dissolved in the driving liquid.
このように、マイクロポンプ11より上流に酸素吸収剤添加部61を配置することにより、ポンプに送液する前に駆動液内に溶存する気体を除去することができ、ポンプ内での気泡に起因する送液の不具合を解消したマイクロチップ検査装置を得ることができる。 As described above, by disposing the oxygen absorbent addition unit 61 upstream of the micropump 11, the gas dissolved in the driving liquid before being sent to the pump can be removed, which is caused by bubbles in the pump. Therefore, it is possible to obtain a microchip inspection apparatus that solves the problem of liquid feeding.
なお、本明細書で言う流路とは、駆動液を貯蔵する駆動液タンクを含み、該駆動液タンクからマイクロポンプ11までの駆動液が存在又は流れる領域をいうものである。 The flow path referred to in the present specification includes a driving liquid tank that stores the driving liquid, and refers to a region where the driving liquid from the driving liquid tank to the micropump 11 exists or flows.
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