JPWO2008013205A1 - Chip-mounting-type integrated processing apparatus, chip-shaped container, and chip-mounted-type integrated processing method - Google Patents

Abstract

It is an object to provide a chip mounting type integrated processing apparatus, a chip-like container, and a chip mounting type integrated processing method that smoothly perform simultaneous mounting operation of a plurality of chips to a plurality of nozzles, and one or more protruding outward And a nozzle head in which two or more nozzles are arranged in a predetermined arrangement pattern, a suction / discharge mechanism, a mounting opening, and a mouth provided at the tip and capable of entering and exiting the fluid. Two or more cylindrical chip-shaped containers, a chip-shaped container accommodating part that accommodates or can be accommodated in a state in which the chip-shaped containers can be attached to the nozzles in a predetermined arrangement pattern, and a nozzle head and a chip-shaped container accommodating part And two or more nozzles provided in the nozzle head, the outer peripheral projections provided on each nozzle by the attachment of the tip-like container are the mounting openings. Peripheral wall and close to or in contact, the distance to at least one of the peripheral projections from a predetermined reference horizontal surface is configured to differ from one another.

Description

  The present invention relates to a chip mounting type integration processing apparatus, a chip-shaped container, and a chip mounting type integration processing method.

  Conventionally, there has been a dispensing device invented and patented by one of the inventors of the present application. The dispensing device attaches a dispensing tip to a lower end of a nozzle in a mounting opening by fitting, and slides a plunger in a cylinder communicating with the nozzle, thereby lowering the lower end in the dispensing tip. This is done by sucking or discharging the liquid through (Patent Document 1 to Patent Document 4).

  These dispensing devices use a cylinder that drives a plunger as a suction and discharge mechanism, but the mechanism such as the plunger is a high-precision processed part such as a syringe, and in particular, the volume change in the cylinder is It is basically integral with the volume change in the dispensing tip and needs to be transmitted so that there is no looseness at the joint between the plunger and the drive device of the plunger. Furthermore, it is necessary to fit the nozzles of these suction and discharge mechanisms, dispensing tips, etc. so that there is no leakage of gas or liquid, and accuracy and structure for watertightness and airtightness are required for manufacturing and quality control. The In particular, when a plurality of dispensing tips are used in an integrated manner, a large force is required to insert and fit a plurality of nozzles into a plurality of dispensing tips all together, for example, a single nozzle. Note: About 1 kilogram of force is required to mount the tip. In the case of 96 pieces, a force close to about 100 kilogram is required, and the O-ring for watertightness and airtightness is severely worn and high quality control is required. It had the problem that there is a possibility of becoming.

Japanese Patent No. 3115501 Japanese Patent No. 3739953 Japanese Patent No. 3630497 Japanese Patent No. 3682302

  Accordingly, a first object of the present invention is to provide a chip mounting type integrated processing apparatus, a chip container, and a chip capable of smoothly performing simultaneous fitting and simultaneous mounting operation of a plurality of chip containers to a plurality of nozzles. It is to provide a wearable integrated processing method. A second object is to provide a chip-mounted integrated processing apparatus, a chip-shaped container, and a chip-mounted integrated processing method that are highly reliable by providing high watertightness and airtightness, and have a long device life and a low quality control burden. Is to provide. A third object is to provide a chip-mounted type integrated processing apparatus, a chip-shaped container, and a chip-mounted type integrated processing method capable of integrating a plurality of processes and performing an efficient and quick process. It is to be.

  According to a first aspect of the present invention, there is provided a nozzle having one or two or more outer peripheral protrusions protruding outward along one or two or more closed outer peripheral bands spaced from each other in the axial direction of the nozzle, and the two or more nozzles Are arranged in a predetermined arrangement pattern, a suction / discharge mechanism that sucks and discharges gas through the nozzle, a mounting opening that can be attached to or attached to the nozzle, and the suction of the gas provided at the tip Two or more substantially chip-shaped containers having a mouth portion through which fluid can be entered and exited by discharging, and a chip that accommodates or can be accommodated in a state where the chip-shaped container can be attached to the nozzle in the predetermined arrangement pattern And a moving means for relatively moving between the nozzle head and the tip-like container receiving portion, and the two or more nozzles provided in the nozzle head are provided with the tip-like container. A plurality of types in which the outer peripheral protrusions provided on the nozzles are in close contact with or in contact with the inner peripheral wall surface of the mounting opening and the distance from a predetermined reference horizontal plane to at least one of the outer peripheral protrusions is different from each other. It is a chip mounting type integrated processing device comprising a group of nozzles.

Here, the “predetermined arrangement pattern” is, for example, a matrix shape, a single row shape, an annular shape, or the like.
“Matrix” refers to a structure in which elements, for example, nozzles, etc., are arranged in a predetermined number of rows and a number of columns at predetermined row intervals and column intervals along two directions of the column direction and the row direction. The column direction and the row direction are usually orthogonal, but are not necessarily limited thereto and may be oblique. The “nozzle” is a portion where fluid is sucked and discharged, and the fluid includes gas and liquid. The nozzle is a flow path communicating with a “suction / discharge mechanism” such as a cylinder having a plunger. Note that “suction / discharge” means suction or / and discharge.

  The “peripheral protrusion” is an annular shape formed along a belt-like outer peripheral band that is closed on the outer peripheral side surface of the nozzle so as to surround the nozzle axis and to protrude outward, radially, or normal from the outer peripheral side surface. Part. The outer circumferential band is preferably formed so as to be sandwiched between planes perpendicular to the nozzle axis. Further, it is preferable that the height of the outer peripheral protrusion in the outward direction is constant around the nozzle. The band width along the axial direction of the outer edge portion that is in close contact with or in contact with the inner peripheral wall surface is, for example, in comparison with the width portion of the outer peripheral band on the outer surface such as a linear or wall thickness width of the chip-shaped container. It is preferable for insertion to be formed small. The outer edge of the outer peripheral projection may be provided with an O-ring along the outer periphery, or a groove may be provided to embed the O-ring to ensure watertightness and airtightness.

  When the outer peripheral protrusion and the inner peripheral wall surface are in close contact with or in contact with each other, the passage of the fluid along the axial direction of the nozzle can be performed by partitioning the space inside the chip-shaped container, particularly the opening for mounting, in the axial direction. It is possible to stop.

  “Closely” means that two objects are in contact with each other with substantially no gap, and in this case, in the entire circumferential length of the outer peripheral protrusion, it is in contact with the inner peripheral wall surface. In this case, the two objects are in contact with each other while allowing the existence of a gap, and in this case, a part of the entire circumferential length of the outer circumferential protrusion is in contact with the inner circumferential wall surface. Therefore, in the case of “close”, the gap between the outer peripheral protrusion and the inner peripheral wall surface is smaller than in the case of “contact”, the resistance force during insertion is large, and there is no fluid leakage or Very small.

  A “chip-shaped container” is a container having an opening for mounting on a nozzle and a mouth for flowing in and out of a fluid at the tip. The mounting opening and the mouth are preferably provided along the axial direction of the container. The material of the chip-like container is, for example, a resin such as polyethylene, polypropylene, polyester, polystyrene, polyvinyl, or acrylic, or an elastic body such as rubber. The chip-like container is preferably transparent or translucent. Since it is “substantially cylindrical”, it is substantially cylindrical or substantially rectangular. The size of the chip-shaped container is, for example, a length from the mouth portion along the mounting opening or in the axial direction of several centimeters to several tens of centimeters, and the volume thereof depends on the length. For example, it is about several microliters to several tens of milliliters. The suction and discharge amount is, for example, about several microliters to several tens of milliliters depending on the volume.

  The chip-shaped container typically includes, for example, a thick tube portion provided with the mounting opening, a thin tube portion having the mouth portion, and a transition portion formed between the thick tube portion and the thin tube portion. It is not limited to a typical dispensing tip, but may be a tube having the same cross-sectional shape along the axial direction as a whole. Moreover, as the shape of the transition portion, for example, a truncated cone shape, a funnel shape, or a step shape is formed. The thick tube and the thin tube are not necessarily limited to a cylindrical shape, and may be a prismatic shape, a polygonal shape, a conical shape, a pyramid shape, or a polygonal pyramid shape.

  Further, by forming the tip of the thin tube to be tapered or sharp, the thin tube is used with respect to a prepack type reagent storage container in which a solution containing a reagent, a sample, or the like is previously stored and the opening is covered with a film. By piercing the film, the solution contained in the container can be sucked through the thin tube.

  Magnetic force means capable of applying and removing a magnetic field in the chip-shaped container may be provided in the nozzle head so as to be outside the chip-shaped container. As a result, for example, a suspension in which a large number of magnetic substances holding biological compounds such as proteins, peptides, amino acids, DNA, RNA, oligonucleotides, sugar chains, etc. are suspended is sucked or discharged into the deformable dispensing tip. At this time, or during storage, a magnetic field can be applied to the inside of the chip and adsorbed on the inner wall of the chip to separate the magnetic substance, and thus the biological compound. The magnetic force means has, for example, two or more magnets provided so as to be able to come into contact with and separate from two or more of the chip-like containers at the same time.

  The “inner peripheral wall surface of the mounting opening” is formed so as to be in close contact with or in contact with the outer peripheral protrusion. Therefore, for example, a cylindrical surface, a cylindrical surface, a cylindrical surface with a step, a cylindrical surface, a tapered surface, a truncated cone surface, a combination of a cylindrical shape and a truncated cone surface, and the like.

  The “predetermined reference horizontal plane” is a horizontal plane that serves as a measurement reference set to define the distance between the outer peripheral protrusions provided on the nozzles attached to the nozzle head. For example, the nozzle head includes a plurality of nozzles. Is a surface that protrudes, a horizontal surface that cuts a plurality of nozzles, a horizontal surface that is set on the stage, and the like. Usually, it is a plane perpendicular to the axial direction of the nozzle. This is because even if the nozzles have the same shape, the relationship between the mounted chip-like container and the outer peripheral protrusion varies depending on the state of being attached to the nozzle head. The total length along the axial direction of the nozzles arranged in the nozzle head, or the distance from the nozzle arrangement surface to the tip of the nozzle is preferably the same. The “distance to the outer peripheral protrusion” is, for example, a distance from the predetermined reference water surface to the center of gravity, upper end, lower end, or predetermined position of the outer peripheral protrusion.

  In the case of “a nozzle having two or more outer peripheral protrusions protruding outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle”, one outer peripheral protrusion between each nozzle In addition to the other outer peripheral protrusions, it is preferable that the distances from the predetermined reference horizontal plane to the outer peripheral protrusions that can be in close contact or in contact with each other include nozzle groups different from each other. As a result, it is possible to prevent a situation in which a resistance force due to a static frictional force or the like between each outer peripheral protrusion and each inner peripheral wall surface is generated simultaneously for each nozzle.

  In addition, when one nozzle has two or more outer peripheral protrusions, the inner peripheral wall surface of the opening for mounting the chip-shaped container is also mutually connected in the axial direction of the container according to each outer peripheral protrusion. Two or more inner peripheral cylindrical wall surfaces (a cross section perpendicular to the axial direction is constant) may be provided, and a space between them may be connected with a tapered surface or a stepped surface.

  Furthermore, when one nozzle has two or more outer peripheral protrusions, even in the same nozzle, the outer peripheral protrusions of the nozzle and the inner peripheral wall surfaces of the chip-like container that are in close contact with or in contact with the outer peripheral protrusions. It is preferable to change the temporal positional relationship according to the movement between the two. As a result, it is possible to prevent a situation in which a resistance force due to a static frictional force or the like between the outer peripheral protrusions and the inner peripheral wall surface of the same nozzle is generated at the same time.

  The arrangement of the nozzles belonging to the plurality of types of nozzle groups is arranged so that the distance between the at least one outer peripheral protrusion of the nozzle and the predetermined reference horizontal plane or the length of the length is uniform without being spatially biased. Is preferred. As a result, the resistance force that the entire nozzle head receives from the entire tip-shaped container dispensing tip during mounting can be received in a dispersed state as much as possible. For example, the nozzle groups are arranged so that the distances or lengths of the adjacent columns (or rows) become longer and shorter, and the nozzles belonging to the same nozzle group are arranged for the elements belonging to each column (row). Furthermore, it is preferable that the nozzle groups are changed and arranged regularly so that the distances or lengths of the adjacent elements of the matrix or other arrangements become longer or shorter. Each nozzle group has at least one nozzle, and preferably the number of nozzles belonging to each nozzle group is the same or substantially the same. As a result, the resistance force can be evenly distributed.

  Here, when the one nozzle is inserted into the mounting opening, the first resistance force received by the outer peripheral projections from the inner peripheral wall surfaces is the static friction coefficient, the outer peripheral protrusion, and the inner peripheral wall surface. Is proportional to the product of the drag in the normal direction. The subsequent second resistance force is proportional to the product of the dynamic friction coefficient and the drag in the normal direction between the outer peripheral protrusion and the inner peripheral wall surface, and the dynamic friction coefficient is greater than the static friction coefficient. Generally small.

  Therefore, when the chip-like container is attached to all the nozzles of the nozzle head at the same time, the operation is as follows. The two or more nozzles provided in the nozzle head are formed of a plurality of types of nozzle groups having different distances from the predetermined reference horizontal plane to the outer peripheral protrusion. Then, for example, the distance from the reference horizontal plane set in the nozzle head is the largest as the nozzle-shaped container mounting openings arranged in correspondence with the nozzle arrangement pattern are moved together in the nozzle insertion direction. The outer peripheral protrusions of the nozzles belonging to the first nozzle group first reach a state in close contact with or in contact with the inner peripheral wall surface of the chip-like container. Until this state is reached, the resistance force that the nozzle receives from the tip-shaped container is 0 or very small. However, when this state is reached, the outer peripheral projection is caused by a collision with the inner peripheral wall surface or a static frictional force. Suspension or insertion speed is drastically reduced due to resistance. When this resistance force is overcome and the insertion into the nozzle proceeds, the insertion operation continues while receiving a resistance force due to a dynamic friction force smaller than the resistance force. Here, the initial resistance force received by the nozzle is proportional to the product of the number of nozzles, the coefficient of static friction, and the drag in the normal direction acting between the outer peripheral protrusion and the inner peripheral wall surface. When the movement of the nozzle head in the insertion direction is continued, it relates to a resistance force having a magnitude using a dynamic friction coefficient instead of the static friction coefficient. In general, since the dynamic friction coefficient is smaller than the static friction coefficient, the resistance force based on the dynamic friction coefficient is smaller than the resistance force based on the static friction coefficient.

  Eventually, the nozzles belonging to the second nozzle group having the outer peripheral protrusion having the next largest distance from the reference horizontal plane are inserted until the outer peripheral protrusion is in close contact with or in contact with the inner peripheral wall surface of the chip-like container. . Then, as described above, the number of nozzles belonging to the second nozzle group, the collision or static friction coefficient between the outer peripheral protrusion and the inner peripheral wall surface, and the normal line between the outer peripheral protrusion and the inner peripheral wall surface. The resistance force proportional to the product of the direction force is added to the resistance force corresponding to the dynamic friction coefficient of the first nozzle group, and the nozzle head receives the resistance force.

  If the movement in the insertion direction is continued as it is, as described above, the second nozzle group receives a resistance force proportional to the dynamic friction coefficient instead of the static friction coefficient.

  It is preferable to further provide a liquid storage section group that can store or store various liquids on the stage on which the chip-shaped container storage section is installed. “Various liquids” include liquids containing various reagents, specimens, chemical substances, or magnetic substances.

  According to a second aspect of the present invention, in the two or more outer peripheral protrusions provided on the nozzle, the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side. In the chip-mounting type integrated processing apparatus, an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion corresponding to the outer peripheral protrusion is formed in the mounting opening of the container.

  Here, the two or more outer peripheral protrusions are provided apart from each other, and the inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusions is, for example, an inner peripheral cylindrical wall surface provided separately in the axial direction of the container (The cross-sectional shape perpendicular to the axial direction is the same), and a tapered surface or a stepped surface is formed between the inner peripheral cylindrical wall surfaces, or a tapered surface in close contact with or in contact with two or more outer peripheral projections Etc.

  According to a third aspect of the present invention, the inner peripheral wall surface includes a plurality of inner peripheral cylindrical wall surfaces provided to be spaced apart from each other corresponding to the outer peripheral protrusion, and each inner peripheral cylindrical wall surface is connected to the outer peripheral protrusion. The chip mounting type integrated processing apparatus is provided in close contact with or in contact with a tapered end surface of the inner peripheral cylindrical wall surface connected to the inner peripheral cylindrical wall surface.

  Therefore, a tapered surface that expands outward is formed at the rear end of the mounting opening. It is preferable that the edge on the front end side of the tapered surface is continuously connected to the edge on the rear end side of the inner peripheral cylindrical wall surface.

  Similarly, for the tapered surface provided between the adjacent inner peripheral cylindrical wall surface on the front end side and the inner peripheral cylindrical wall surface on the rear end side, the front end edge of the tapered surface is the inner peripheral cylinder on the front end side. It is preferable to continuously connect with the rear end edge of the cylindrical wall surface and continuously connect with the rear end edge of the tapered surface and the front end edge of the inner peripheral cylindrical wall surface on the rear end side.

  Thereby, it is possible to prevent a situation in which a resistance force due to a static frictional force or the like between each outer peripheral protrusion and the inner peripheral cylindrical wall surface is generated at the same time for the same nozzle.

  In the case where the nozzle is provided with two outer peripheral protrusions axially spaced from each other, and the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side. Is a rear end side in close contact with or in contact with a front end side inner peripheral cylindrical wall surface and a rear end side outer peripheral protrusion in the mounting opening of the tip-shaped container. The inner peripheral cylindrical wall surface is provided apart, and between the front end side inner peripheral cylindrical wall surface and the rear end side inner peripheral cylindrical wall surface, and on the rear end side of the rear end side inner peripheral cylindrical wall surface, It is preferable to form a tapered surface.

  According to a fourth aspect of the present invention, the moving means includes at least a structure of the nozzle belonging to the nozzle group and each of the nozzles after each nozzle of the nozzle head reaches a position where the nozzle can be inserted into the mounting opening of the chip-like container. A chip-mounted integrated processing apparatus that relatively moves between the nozzle head and the chip-shaped container housing so that each nozzle is inserted into the mounting opening based on the number of nozzles belonging to the nozzle group. is there.

  Such movement of the moving means is controlled by the control unit. The control unit further controls the movement between the nozzle head and the chip-shaped container housing unit, further the number or structure of the chip-shaped containers, the liquid to be sucked and discharged, the substance contained therein, the amount thereof, It can be controlled based on the storage position, its temperature or its concentration, processing details, or instructions. Substances include various chemical substances including not only biological substances such as nucleic acids, proteins, sugar chains and amino acids but also metals.

  5th invention has a desorption part which desorbs the chip-shaped container with which each nozzle was mounted | worn all at once, and this desorption part is larger than the maximum outer diameter or the maximum width of the horizontal cross section of the said nozzle, A desorption plate in which holes or gaps having a diameter or width smaller than the maximum outer diameter or maximum width of the horizontal cross section of the container are formed according to the predetermined arrangement pattern, and the plate surface of the desorption plate has the predetermined reference A chip-like container that is provided in parallel to a horizontal plane and is movable relative to the nozzle along the axial direction of the nozzle so that the axis of each nozzle passes through the hole or the gap. .

  Here, the “maximum outer diameter or maximum width” is the largest of the outer diameters or widths of the horizontal cross section perpendicular to the axial direction existing in the nozzle or tip-shaped container. The “hole” is a closed gap that is formed so as to surround the normal direction of the desorption plate provided for each nozzle, and the nozzle can be inserted into the hole from the normal direction of the desorption plate. The “gap” is a gap provided in the desorption plate and opened in the lateral direction of the desorption plate, and the nozzle can be inserted into the gap from the normal direction of the desorption plate and from the portion opened in the lateral direction. The “diameter” corresponds to a circumferential shape, and the “width” corresponds to a shape other than the circumferential shape. Since it is “relative”, for example, first, when the nozzle is stopped and the desorption plate is moved downward from the upper side of the chip-shaped container, and secondly, the desorption plate is fixed and the nozzle is moved. There are a case where the chip-shaped container is moved upward from the upper side and a case where both are moved third. For example, in the first case, the desorption plate may be supported by a nozzle head and provided so as to be movable along the axial direction of the nozzle with respect to the nozzle head. In the second case, the desorption plate is fixedly provided on the stage provided with the chip-shaped container housing portion, and a gap is provided in the desorption plate, and the nozzle is laterally disposed in the gap of the desorption plate. In some cases, the tip-shaped container is detached from the nozzle by raising the nozzle head by the moving means. Since it is “formed according to the predetermined array pattern”, holes are formed so as to have the same array pattern as the predetermined array pattern, for example, the same number of rows, the same number of columns and the same row spacing, column spacing, An array pattern corresponding to the array pattern, for example, several rows (or several rows) of long holes or voids along the column direction (or row direction) can be inserted into the nozzles of the number of rows (or several columns). And so on.

  6th invention is the substantially cylindrical cylindrical container which can accommodate a liquid inside, 1 or 1 which protruded outward along two or more closed outer peripheral belts mutually spaced apart to the axial direction An opening for mounting that can be attached to a nozzle having two or more outer peripheral projections, and a mouth that is provided at the tip of the container and through which the fluid can be introduced and discharged by suction and discharge of gas through the nozzle; The mounting opening includes an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion of the nozzle when mounted on the nozzle, and a tapered connection that is provided on the rear end side of the inner peripheral wall surface and is connected to the inner peripheral wall surface. A chip-like container having a tapered surface.

  According to a seventh aspect of the invention, the inner peripheral wall surface is provided corresponding to one or more outer peripheral protrusions protruding outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle. A plurality of inner peripheral cylindrical wall surfaces which are in close contact with or in contact with the outer peripheral projections by being mounted on the nozzles and are spaced apart from each other in the axial direction of the container, The inner peripheral length of the inner wall surface is shorter than the inner peripheral length of the inner peripheral cylindrical wall surface on the rear end side, and the tapered shape is formed on the rear end side of the inner peripheral cylindrical wall surface. It is a container.

  Of course, even in the plurality of outer peripheral protrusions provided on the nozzle, the outer peripheral length of the outer peripheral protrusion on the front end side is formed shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side, It corresponds to the circumference.

  According to an eighth aspect of the present invention, in the two outer peripheral protrusions provided in the nozzle and spaced apart from each other in the axial direction, the outer peripheral length of the outer peripheral protrusion on the front end side is greater than the outer peripheral length of the outer peripheral protrusion on the rear end side. The opening for mounting of the chip-like container is in close contact with or in contact with the outer peripheral protrusion, and is provided in two inner peripheral cylindrical shapes provided apart from each other in the axial direction of the container. The inner peripheral length of the inner peripheral cylindrical wall surface of the front end side is shorter than the inner peripheral length of the inner peripheral cylindrical wall surface of the rear end side, and the rear end side and rear end side inner side of the front end inner peripheral cylindrical wall surface A tapered surface is formed between the circumferential cylindrical wall surface and the inner circumferential cylindrical wall surface on the distal end side, and the distal inner circumferential cylindrical wall surface is attached to the nozzle on the distal end side of the nozzle. The rear end side inner peripheral cylindrical wall surface is in close contact with the outer peripheral protrusion, and is a chip-shaped container that comes into contact with the outer end protrusion on the rear end side.

  According to a ninth aspect of the invention, there is provided a nozzle having one or two or more outer peripheral projections projecting outward along one or two or more closed outer peripheral bands that are spaced apart from each other in the axial direction, and the two or more nozzles are predetermined. Nozzle heads arranged in an array pattern, a suction / discharge mechanism that sucks and discharges gas through the nozzles, a mounting opening that can be attached to the nozzle, and fluid in / out by the gas suction / discharge provided at the tip Two or more substantially cylindrical chip-like containers having a mouth portion that is capable of being arranged, the chip-like containers are arranged in the predetermined arrangement pattern, and are accommodated in a state that can be attached to the nozzles, Moving means for moving the nozzle head relative to the tip-shaped container housing portion, and the two or more nozzles provided in the nozzle head are arranged in front of each nozzle by mounting the tip-shaped container. The outer peripheral protrusion is in close contact with or in contact with the inner peripheral wall surface of the mounting opening, and the outer peripheral protrusion is composed of a plurality of types of nozzle groups having different distances from a predetermined reference horizontal plane to the outer peripheral protrusion. The nozzle head is moved to a position where each nozzle of the head can be inserted into the mounting opening of the chip-shaped container accommodated in the chip-shaped container accommodating portion, and the nozzle head is lowered to form the chip shape. A chip mounting type integration processing method including a step of mounting a container on each nozzle of the nozzle head.

  Here, the lowering of the nozzle head is preferably performed based on at least the structure of the nozzles belonging to each nozzle group and the number of nozzles belonging to each nozzle group. As a result, it can be mounted with an optimum force.

  A tenth aspect of the present invention has a detachable part for detaching the chip-like containers attached to the nozzles all at once, and the detachable part is larger than the maximum outer diameter or maximum width of the horizontal cross section of the nozzle, A desorption plate provided with holes or gaps having a diameter or width smaller than the maximum outer diameter or maximum width of the container in accordance with the predetermined arrangement pattern, the plate surface of the desorption plate being parallel to the predetermined reference horizontal plane The tip-like container mounted on the nozzle by being moved relative to the nozzle along the axial direction of the nozzle so that the axis of each nozzle passes through the hole or the gap. This is a chip mounting type integration processing method including a step of removing and attaching the chip.

  According to the first invention, the sixth invention, or the ninth invention, by mounting two or more nozzles belonging to a plurality of nozzle groups in the nozzle head, mounting or demounting between all nozzles and all chip-shaped containers is achieved. The resistance force based on the sum of the static frictional forces of all the nozzles can be obtained by utilizing the fact that the pattern of resistance force generated between the outer peripheral protrusion and the inner peripheral wall surface associated with the relative movement at the time is different for each nozzle group. , It is possible to avoid concentrating on the nozzle head at the same time or at the same position. Therefore, at the time of mounting or detaching the tip-shaped container on or from the nozzle, a large force corresponding to the sum of the resistance forces based on the static friction force is dispersed to the number of times corresponding to the number of groups of the nozzle group, whereby the nozzle The maximum force applied to the head can be reduced, the force applied to each outer protrusion can be reduced, the wear of each outer protrusion can be prevented, the operating cost can be reduced, and the life of the device can be extended. Management can be facilitated.

  In addition, by automatically mounting or removing the tip-shaped container on the nozzle, various processes can be performed consistently, which is suitable for automation. Furthermore, since each outer peripheral protrusion is provided along the closed outer peripheral band, water-tightness and airtightness outside the chip-like container are high.

  According to the second invention, the third invention, the seventh invention, or the eighth invention, in addition to the effects described above, the outer peripheral lengths of the plurality of outer peripheral protrusions are formed to be shorter toward the tip. Thus, it is possible to smoothly insert a nozzle having a plurality of outer peripheral protrusions.

  Further, by providing an outer peripheral protrusion provided along a plurality of closed outer peripheral bands and an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion, liquid or gas leakage can be prevented even more reliably. Can prevent cross-contamination and is highly reliable.

  Furthermore, when a plurality of outer peripheral protrusions are provided, it is inserted in close contact with one inner peripheral wall surface and brought into contact with the other inner peripheral wall surface, thereby reducing resistance due to friction with the inner peripheral wall surface. However, as compared with the case where only one outer peripheral projection is provided, this prevents the rattling from occurring when the nozzle is mounted due to the axial displacement between the nozzle and the tip-shaped container, and allows smooth insertion. be able to.

  According to the third invention, the sixth invention, or the eighth invention, in addition to the above-described effects, an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion of the nozzle is provided, and the rear end side of the inner peripheral wall surface In addition, by forming the tapered surface, it is possible to smoothly insert the nozzle into the mounting opening of the tip-shaped container. Further, it is preferable that the tip of the nozzle is also tapered. By providing an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion of the nozzle, it is possible to prevent the flow of gas or liquid from the tip-shaped container beyond the outer peripheral protrusion of the nozzle.

  According to the fourth invention, in addition to the effects described above, an appropriate magnitude can be obtained by obtaining in advance the force to be applied to the nozzle head based on the structure of the nozzles belonging to the nozzle group and the number of nozzles belonging to each nozzle group. Therefore, it is possible to prevent excessive wear of the outer peripheral projections, thereby extending the life of the apparatus.

  According to 5th invention or 10th invention, as a desorption part, a predetermined | prescribed hole or clearance gap is provided in a desorption board, and a simple structure is provided by providing between the said nozzle and a desorption board so that relative movement is possible. Thus, the nozzle tips attached to the nozzles can be detached and attached collectively. Further, since the desorption plate is provided in parallel with the predetermined reference horizontal plane, a large force corresponding to the total resistance force based on the static friction force is applied when the tip-shaped container is attached to or detached from the nozzle. By distributing the number of nozzles corresponding to the number of groups, the maximum force applied to the nozzle head and the detachable plate is reduced, the force applied to each outer peripheral protrusion is reduced, and wear of each outer peripheral protrusion is prevented. Therefore, the operation cost can be reduced and the life of the apparatus can be extended, thereby facilitating product management.

  Next, a chip mounting type integrated processing apparatus, a chip-shaped container, and a chip mounting type integrated processing method according to an embodiment of the present invention will be described based on the drawings.

FIG. 1 schematically shows a chip-mounted integrated processing apparatus 10 according to an embodiment of the present invention.
The chip-mounted integrated processing apparatus 10 has a total of 96 substantially cylindrical nozzles 14a, 14b, 14c, and 14d, and the nozzles 14a, 14b, 14c, and 14d are arranged in a matrix of 12 rows × 8 columns. The nozzle head 12 arranged so as to protrude downward from the nozzle arrangement plate 20 having the predetermined reference horizontal plane on the lower surface, and the suction / discharge mechanism (16, 18, 22, 24) for sucking and discharging gas through the nozzle. , 26, 28, 30) and moving means (34, 36, 38, 40) for moving the nozzle head 12 relative to the chip housing portion.

  As will be described later, the chip mounting type integrated processing apparatus 10 includes a dispensing chip as a chip-like container mounted on the nozzles 14a, 14b, 14c, and 14d, and the dispensing chip according to the arrangement pattern. It has a chip accommodating part as a chip-like container accommodating part which is arranged and accommodated in a state where it can be attached to the nozzles 14a, 14b, 14c and 14d.

  The suction / discharge mechanism (16, 18, 22, 24, 26, 28, 30) is arranged in a matrix-like arrangement pattern of 12 rows × 8 columns of the arrangement pattern provided in the nozzle head 12, 96 cylinders 16 communicating with the nozzles 14a, 14b, 14c, 14d, 96 rods 18 for driving a plunger (not shown) slidably inserted into the cylinders 16, and the 96 cylinders The rod driving plate 22 for connecting the rods of the two, and the rod driving plate 22 are connected to drive the rod driving plate 22 and thus each rod in the vertical direction (Z-axis direction) at the same time. A columnar actuator 24 is connected to the actuator 24, and is engaged with a ball screw 28 to drive the actuator 24 in the vertical direction. A nut portion 26 which advances move, and a ball screw 28 which is rotationally driven by a suction and discharge motor 30. The nozzle head support 32 supports the suction / discharge motor 30 and the nozzle array plate 20 and supports the actuator 24 so as to move up and down.

  The moving means (34, 36, 38, 40) has an X-axis having a ball screw that rotationally drives the nozzle head 12 by an X-axis motor (not shown) in the X-axis direction (row direction of the array pattern). A Y-axis drive mechanism (not shown) having a ball screw rotated by a drive mechanism (not shown) and a Y-axis motor (not shown) and driving in the Y-axis direction (column direction of the array pattern) A frame 33 that is movable in the X-axis direction and the Y-axis direction, a ball screw 34 that is rotatably supported by the frame 33 and extends in the vertical direction (Z-axis direction), and a ball screw 34. The nut portion 36 that is screwed together and connected to the nozzle head support 32 and translates the nozzle head support 32 and thus the nozzle head 12 in the vertical direction by rotation of the ball screw 34, and the frame Having a Z-axis motor 40 which is supported rotationally driving the ball screw 34 to 33, a connector 38 for connecting the motor shaft of the Z-axis motor 40 ball screw 34. Although not shown, the apparatus includes a control unit for controlling the moving unit, the suction / discharge mechanism, and the like. The control unit includes, for example, an information processing device including a CPU and a memory, a data input device such as a mouse and a keyboard, a display device such as a liquid crystal panel, a data output device such as a printer, a communication means, or a CD, DVD, flexible disk, etc. The external memory drive device.

  FIG. 2 is a plan view of the nozzle array plate 20 provided in the nozzle head 12 as viewed from below. The lower surface of the nozzle array plate 20 corresponds to the predetermined reference horizontal plane. The 96 nozzles 14a, 14b, 14c, and 14d are 24 nozzles each of which belongs to four types of nozzle groups, which will be described later, and each adjacent element of an array pattern of 12 rows × 8 columns. Each of the nozzles 14a and the first nozzle belonging to the first nozzle group has an order determined according to a distance or a length between a front end side outer peripheral protrusion described later and a predetermined reference horizontal plane, i.e., alternately long and short. The nozzles 14c belonging to the third nozzle group, the nozzles 14b belonging to the second nozzle group, and the nozzles 14d belonging to the fourth nozzle group are sequentially arranged. Therefore, the number of nozzles belonging to each nozzle group is 24. As a result, the resistance force received by the entire nozzle head 12 from the entire dispensing tip 46 is dispersed as much as possible.

  As another arrangement method, for example, 12 nozzles belonging to the same nozzle group are arranged for each row. Nozzles 14a belonging to the first nozzle group for the first nozzle group to the fourth nozzle group formed according to the order of the distance or length between the nozzle tip side outer peripheral protrusion and a predetermined reference horizontal plane, which will be described later, Twenty-four nozzles 12 are arranged in the first row and the fifth row, and 24 nozzles 14b belonging to the second nozzle group are arranged in the third row and the seventh row, respectively, 24 nozzles 14b and belong to the third nozzle group. There may be a case where 24 nozzles 14c are arranged in 12 rows in the second row and the sixth row, and 24 nozzles 14d belonging to the fourth nozzle group are arranged in 12 rows in the fourth row and the eighth row, respectively.

  In FIG. 3, the nozzle head 12 schematically shown, and 96 dispensing tips 46 of the same shape that can be mounted on the nozzles 14a, 14b, 14c, and 14d of the nozzle head 12 are arranged in the arrangement pattern, that is, The chip accommodating part 42 accommodated in the state which can be mounted | worn with the said nozzles 14a, 14b, 14c, and 14d horizontally arranged in the matrix form of 12 rows x 8 columns is shown.

  The tip accommodating portion 42 includes an upper plate 44 having 96 through holes arranged in a 12-row × 8-column arrangement pattern for inserting and supporting the dispensing tips 46, and the upper plate The four struts 48 having a height capable of supporting the upper plate 44 horizontally at the four corners 44 and supporting the dispensing tips 46 through the through holes of the upper plate 44, and the struts 48 at the four corners. And a lower plate 50 for supporting it so as to stand upright. The size of the through-hole is such that the main body of the dispensing tip 46 (a thick tube portion 62, a thin tube portion 66 and a transition portion 68 described later) can be inserted, but a plurality of protrusions provided at the upper end are formed. The protrusion forming portion 47 is formed in a size that cannot be inserted. A microplate (not shown) in which wells that can store various liquids or can be stored are arranged in the array pattern is provided on the stage on which the chip storage portion 42 is placed.

  FIG. 4 shows a state in which the nozzle head 12 and the chip accommodating portion 42 are looked up from below. Each of the 96 nozzles 14a, 14b, 14c, and 14d is vertically downward from a reference horizontal plane that is the lower surface of the nozzle array plate 20 of the nozzle head 12 via 96 nozzle support members 51 having the same shape. It is provided so as to protrude.

  FIG. 5 shows a state in which the dispensing tip 46 is attached to the nozzles 14 a, 14 b, 14 c, 14 d provided in the nozzle head 12.

  FIG. 6 shows a state in which the dispensing tip 46 is attached to one type of nozzle 14 a provided in the nozzle head 12.

  The nozzle 14a protrudes from the lower surface of the nozzle array plate 20 of the nozzle head 12, and is connected to the lower side of the nozzle support member 51 provided therein with a cylindrical channel 51a communicating with the nozzle 14a. Is provided. The nozzle 14a protrudes outward with respect to the main body 56a at a constant height along a cylindrical main body 56a and an annular outer peripheral band closed so as to surround the axis of the nozzle, and is separated from each other in the axial direction. The rear end side outer peripheral protrusion 52a and the front end side outer peripheral protrusion 54a are provided. The width of the outermost edge of the outer peripheral projection is narrower than the width of the outer peripheral band on the outer peripheral surface, and is formed, for example, about the thickness of the dispensing tip 46. The front end side outer peripheral protrusion 54a has a shorter outer peripheral length than the rear end side outer peripheral protrusion 52a. The tip 58a of the nozzle 14a has a tapered surface tapered toward the tip 58a of the nozzle 14a on the lower side of the tip-side outer peripheral projection 54a.

  The dispensing tip 46 attached to the nozzle 14a is formed in a substantially cylindrical shape as a whole, provided at the rear end thereof, attached to the nozzle 14a (or 14b, 14c, 14d), or an attachment opening that can be attached. Part 60, a mouth part 64 provided at the tip and capable of entering and exiting the fluid by suction and discharge of the gas by the nozzle 14a (or 14b, 14c, 14d), and the mounting opening part 60 are provided on the upper side. The thick pipe portion 62 and the mouth portion 64 are provided at the lower end, are formed narrower than the thick pipe portion 62, and have a substantially tapered thin tube portion 66, and between the thick tube portion 62 and the thin tube portion 66. And a funnel-shaped transition portion 68 provided.

  The mounting opening 60 is provided at the front end side inner peripheral cylindrical wall surface 72 in close contact with the front end side outer peripheral protrusion 54a, and spaced from the front end side inner peripheral cylindrical wall surface 72. The rear end side outer peripheral protrusion A rear end-side inner cylindrical wall surface 70 in contact with the portion 52a, and a taper surface tapered downward between the front-end side inner peripheral cylindrical wall surface 72 and the rear-end side inner peripheral cylindrical wall surface 70. 76 is formed, and a taper surface 74 tapering downward is also formed on the rear end side of the rear end side inner peripheral cylindrical wall surface 70. Further, the rear end of the outer wall surface of the thick tube portion 62 has a ridge forming portion 47 provided with a plurality of ridges along the axial direction.

  FIG. 7 shows a case where the same type of dispensing tip 46 is attached to each of the nozzles 14a, 14b, 14c, and 14d belonging to the four types of nozzle groups of the nozzle head 12.

  FIG. 7 shows a nozzle 14a belonging to the first nozzle group to which the dispensing tip 46 is attached, a nozzle 14b belonging to the second nozzle group, a nozzle 14c belonging to the third nozzle group, and a nozzle belonging to the fourth nozzle group. 14d is shown.

The distances from the lower surface of the nozzle array plate 20 to the rear end side outer peripheral projections 52a, 52b, 52c, 52d as the predetermined reference horizontal planes are U _a , U _b , U _c , U _d , respectively. When the distances to the tip-side outer peripheral projections 54a, 54b, 54c, 54d are L _a , L _b , L _c , L _d , the following relational expressions are established. That is, U _a <U _b <U _c <U _d , and L _a > L _b > L _c > L _d . Further, U _d <L _d . Thus, when the nozzles 14a, 14b, 14c, and 14d provided in the nozzle head 12 are mounted, it is possible to prevent all the nozzles from receiving the maximum resistance force at the same time and to divide into four stages.

Further, in particular, the front end side outer peripheral protrusions 54a, 54b, 54c, 54d are not only the front end side inner peripheral cylindrical wall surface 72, but the rear end side outer peripheral protrusions 52a, 52b, 52c, 52d are rear end side inner peripheral cylindrical shapes. In close contact with the wall surface 70, from the reference horizontal surface to the rear end edge of the inner cylindrical wall surface 70 on the rear end side when the dispensing tip 46 is completely attached to the nozzle 14a (14b, 14c, 14d). Assuming that the distance is U _t and the distance to the rear end edge of the inner cylindrical wall surface 72 on the front end side is L _t , U _a -U _t and L _a -L _t are different from each other, and U _b -U _t L _b −L _t is set differently, U _c −U _t is set different from L _c −L _t, and U _d −U _t is set different from L _d −L _t . Accordingly, when the dispensing tip is mounted, it is possible to prevent the rear end side outer peripheral protrusion and the front end side inner peripheral cylindrical protrusion from simultaneously receiving the maximum resistance force from the inner peripheral wall surface in the same nozzle.

  Next, the operation of mounting the dispensing tip 46 on the nozzles 14a, 14b, 14c, 14d according to the embodiment of the present invention will be described based on the drawings.

As shown in FIGS. 1, 2, 3, and 4, the nozzle head 12 is moved up to the upper part of the chip accommodating portion 42, and an X-axis drive mechanism (not shown) and a Y-axis drive mechanism of the moving unit. It is moved using (not shown). Next, the Z-axis motor 40 and the ball screw 43 are driven to lower the nozzle head support 32 and the nozzle head 12 all at once toward the chip housing portion 42. Until the outer peripheral protrusions of any one of the nozzles 14a, 14b, 14c, and 14d come into contact with any one of the inner peripheral cylindrical wall surfaces of the dispensing tip 46, these nozzles, and thus the nozzle head 12, are dispensed. The resistance force received from the chip 46 is 0 or very small. The nozzles provided in the nozzle head 12 are formed of four types of nozzle groups having different distances from the reference horizontal plane to the outer peripheral protrusion, and therefore among the nozzles 14a, 14b, 14c, and 14d, the distal end side outer peripheral protrusion 54a of the nozzle 14a belonging to the first nozzle group having the largest L _a distance from said reference horizontal surface, first contacts with the dispensing distal end side inner tubular wall surface 72 of the chip 46 Descent to position. Resistive force based on the occurrence of collision and drag due to the narrowing of the distal inner cylindrical wall surface 72 in close contact between the distal outer circumferential protrusion 54a and the distal inner cylindrical wall surface 72 and the static frictional force between the objects. the p _a 1 nozzle 14a is subjected.

Accordingly, as shown in FIG. 8, the nozzle head 12 such that the nozzle 14a 24 inborn, it is assumed that P a ₌ 24p a _(not differ by shape between the tolerance and the nozzle groups between products. Hereinafter the same ) This force P _a has a static friction coefficient M, is related to the product MN of the normal direction of the drag N (holding force) acting between the outer peripheral projection and the inner peripheral tubular wall.

At that time, the rear end side inner tubular wall 70, a nozzle 14a, 14b, 14c, of the 14d, after the nozzle 14d belonging to the fourth nozzle group having the largest _{U d} distance from said horizontal reference plane Even if the end-side outer peripheral protrusion 52d first descends to a position where it comes into contact with the rear-end-side inner peripheral cylindrical wall surface 70 of the dispensing tip 46, the rear-end-side outer peripheral protrusion 52d and the rear-end side It is assumed that the resistance force due to friction between the inner peripheral cylindrical wall surface 70 is smaller than the resistance force due to friction between the front end side outer peripheral protrusion 54d and the front end side inner peripheral cylindrical wall surface 72. The resistance force based on the side outer peripheral protrusions 52a, 52b, 52c, 52d and the rear end side inner peripheral cylindrical wall surface 70 is ignored in the following description. That is, the resistance force due to friction is small because, for example, the state between the rear end side outer peripheral protrusion and the rear end side inner peripheral tubular shape is not a close state but a contact state, and the drag is smaller than that of the latter. The friction coefficient may be smaller than the latter depending on the combination of the materials in contact with each other, the presence or absence or quality of the lubricating oil, the smoothness of the surface, the cleanliness, the material, and the like.

Eventually, the force of the nozzle head 12 when lowering proceeds overcome this resistance force, so that the resistive forces R _a by a small dynamic friction force than the resistive force. The resistance force R _a is a dynamic friction coefficient m, related to the product 24mN the normal direction of the drag (corresponding to clamping force) N acting between said inner tubular wall surface and the outer peripheral projection. In general, since the dynamic friction coefficient m is smaller than the static friction coefficient M, the resistance force based on the dynamic friction coefficient is smaller than the resistance force based on the static friction coefficient.

Eventually, the distal end side outer circumferential protrusion 54b of the nozzle 14b belonging to the second nozzle group having a distance next larger distance L _b from the reference horizontal plane, and the dispensing tip side inner circumferential cylindrical wall 72 of the tip 46 It descends to the position where it touches. Collision due to the narrowing of the distal end inner peripheral cylindrical wall surface 72 in close contact between the distal end outer peripheral projection 54b and the distal end inner peripheral cylindrical wall surface 72, and the resistance based on the static frictional force between the objects. a force _{p b} nozzle 14b receives. Therefore, the nozzle head 12 having 24 such nozzles 14b receives _a force of P _b = 24p _b in addition to _Ra . Therefore, the force P _ab received by the nozzle head 12 at this time is P _ab = R _a + P _b .

Eventually, when the force of the nozzle head 12 overcomes this resistance force and the descent proceeds, the nozzle head 12 receives a resistance force _Rb due to a dynamic friction force smaller than the resistance force with respect to the nozzle 14b. This resistance force _Rb is related to the product 24 mN of the dynamic friction coefficient m and the normal direction drag N acting between the outer peripheral projection and the inner peripheral cylindrical wall surface. Therefore, as described above, the resistance force based on the dynamic friction coefficient is smaller than the resistance force based on the static friction coefficient.

Therefore, the force R _{ab received} by the entire nozzle head 12 is R _ab = R _a + R _b , and P _ab > R _ab .
Similarly, when the descent proceeds and the tip outer peripheral projection 54c of the nozzle 14c belonging to the third nozzle group reaches the tip inner cylindrical wall surface 72, P _abc = R _a + R _b + P _c (= 24p _c, p _c becomes a force of resistance) to one nozzle 14c based on collision or static friction coefficient is subjected to the nozzle head 12 is subjected. When the lowering of the nozzle head 12 further proceeds by overcoming the resistance force, the resistance force received by the nozzle head 12 is R _abc = R _a + R _b + R _c (= dynamic friction coefficient m received by the nozzle 14c belonging to the third nozzle group) wherein the P less than _c) in resistance 24mN-based. Therefore, P _abc > R _abc .

Further, when the nozzle head 12 descends and the tip side outer peripheral protrusion 54d of the nozzle 14d belonging to the fourth nozzle group reaches the tip side inner peripheral cylindrical wall surface 72, P _abcd = R _a + R _b + R _c + P _The nozzle head 12 receives a force of _d (= 24 p _d , p _d is a resistance force received by one nozzle 14 d based on a collision or static friction coefficient). When the nozzle head 12 further descends by overcoming the resistance force, the resistance force received by the nozzle head 12 is R _abcd = R _a + R _b + R _c + R _d (= the nozzle 14d belonging to the fourth nozzle group). wherein the P less than _d) with resistance 24mN based on dynamic friction coefficient m receiving. Therefore, P _abcd > R _abcd . As a result, as shown in FIG. 5, the 96 dispensing tips 46 are attached to the nozzles 14 a, 14 b, 14 c and 14 d of the nozzle head 12.

On the other hand, as in the prior art, the nozzles belonging to the first nozzle group, for example, in the case where the distal end side outer circumferential projections, only the nozzle 14a having the L _a is provided by 96 the arrangement pattern in the nozzle head 12, The distal end side outer circumferential protrusion 54a comes into contact with the distal end side inner circumferential cylindrical wall surface 72 of the dispensing tip 46 all at once. Therefore, as shown in FIG. 8, the force p ₀ received by one nozzle is related to the resistance force based on the collision or static friction coefficient, that is, mN, and the force P ₀ received by the nozzle head is P ₀ = 96p ₀ . This will generally be greater than the P _abcd containing a force based on the dynamic friction coefficient.

  9 to 11 schematically show chip-mounted integrated processing apparatuses 100 and 110 according to the second and third embodiments of the present invention. The same reference numerals as those of the chip-mounted integrated processing apparatus 10 according to the first embodiment shown in FIG. 1 to FIG. Unlike the chip-mounted integrated processing apparatus 10 described above, the chip-mounted integrated processing apparatuses 100 and 110 are provided with detachable portions 11 and 111.

  The detachable part 11 of the chip mounting type integrated processing apparatus 100 according to the second embodiment shown in FIG. 9 is supported by the nozzle head 12, and a total of 96 nozzles 14a, The detachable plate 15 provided so as to be movable relative to 14b, 14c, 14d is parallel to the nozzle array plate 20 corresponding to a predetermined reference horizontal surface of the nozzle head 12, and the nozzle array plate 20 Is located above the dispensing tip 46 to be attached to each nozzle 14a, 14b, 14c, 14d. Therefore, the desorption plate 15 is moved to this position before the dispensing tip 46 is attached.

  The desorption plate 15 of the desorption portion 11 is larger than the rear end side outer peripheral projections 52a, 52b, 52c, 52d corresponding to the maximum outer diameter of the horizontal cross section of the nozzles 14a, 14b, 14c, 14d, A hole 13 having a diameter smaller than the protrusion forming portion 47 of the mounting opening 60 corresponding to the maximum width of the dispensing tip 46 as the tip-shaped container is the nozzle 14a, 14b, 14c, 14d. The array pattern formed on the array plate 20, that is, a matrix of 12 rows × 8 columns, is formed with the same row spacing and column spacing as those formed on the nozzle array plate 20. The detaching plate 15 is provided in parallel to the nozzle array plate 20 corresponding to the predetermined reference horizontal plane, and the axes of the nozzles 14a, 14b, 14c and 14d pass through the holes 13 by a moving mechanism (not shown). The nozzle is movably provided along the axial direction of the nozzle.

  The detachable part 111 of the chip mounting type integrated processing apparatus 110 according to the third embodiment shown in FIGS. 10 and 11 is fixedly provided on the stage on which the chip accommodating part 42 is provided. , A desorption plate 19 formed in a comb-like shape, and a support plate 21 for supporting the desorption plate 19 on a stage. The plate surface of the detachable plate 19 is provided parallel to the nozzle array plate 20 corresponding to a predetermined reference horizontal surface of the nozzle head 12. Reference numeral 17 denotes an opening of a tip accommodating portion that is embedded in the stage and accommodates the dispensing tip 46.

  The detachable plate 19 of the detachable portion 111 is larger than the rear end side outer peripheral protrusions 52a, 52b, 52c, 52d corresponding to the maximum outer diameter of the horizontal cross section of the nozzles 14a, 14b, 14c, 14d, A plurality of gaps (12 in this example) having a smaller width than the protrusion forming portion 47 of the mounting opening 60 corresponding to the maximum width of the dispensing tip 46 as the tip-shaped container, and the detachable plate 19 A plurality of comb-tooth members 23 (11 in this example) sandwiched between those opened in one side of the nozzle array plate 20 are arranged in an array pattern, that is, in a matrix of 12 rows × 8 columns. Correspondingly, they are formed along the row direction so as to cover the same row interval and the length in the row direction as those formed on the nozzle array plate 20. The detaching plate 15 is provided in parallel to the nozzle array plate 20 corresponding to the predetermined reference horizontal plane, and the axes of the nozzles 14a, 14b, 14c and 14d pass through the holes 13 by a moving mechanism (not shown). Is formed.

In order to detach the dispensing tip 46 attached to the nozzles 14a, 14b, 14c, 14d using the detaching part 111, the nozzle head 12 is moved by the moving means, and the nozzles 14a, 14b, 14c and 14d are inserted by moving in the row direction in the gap of the detaching plate 19 above the mounted dispensing tip 46. Next, the moving means applies the force in the direction in which the dispensing tip 46 is detached from the nozzle, that is, the nozzle head 12 is moved upward along the axial direction of the nozzle or the dispensing tip, and is scraped off. Configure as follows.
In this way, even when detaching, a plurality of types having different temporal positional relationships in which the resistance force generated by the close contact or contact between the inner peripheral wall surface of the tip-shaped container and the outer peripheral protrusion of the nozzle is applied to the detachable portion. The tip-shaped container can be detached from the nozzles with a small force.

  Each embodiment described above is specifically described in order to better understand the present invention, and does not limit other embodiments. Therefore, the present invention can be changed without changing the gist of the invention. For example, in the embodiment described above, the nozzle mainly has two outer peripheral protrusions, which are in contact with each other between the upper outer peripheral protrusion and the upper inner peripheral wall surface, and the lower outer peripheral protrusion and the lower outer peripheral protrusion. The case of close contact with the inner peripheral wall surface has been described. However, the present invention is not limited to this case. For example, when one or three outer peripheral protrusions are provided in one nozzle, or the upper outer peripheral protrusion is provided. And the upper inner wall surface may be in close contact with each other.

  Further, the chip-shaped container is not limited to the above-described one, and may have a step at the transition part or a step other than the transition part. Further, instead of a dispensing tip, a tip-like container in which particles, blocks, elongated shapes, or wound carriers are enclosed may be used.

The arrangement pattern is not limited to 12 rows × 8 columns. For example, there are those in which 4, 6, 8, 12, 96, 384, etc. are arranged in a single line or a matrix or other shapes.
Furthermore, a short pipe having a smaller diameter such as stainless steel may be further fitted into the mouth of the thin tube of the tip-shaped container so as to increase the dispensing accuracy.
It should be noted that all references to spatial relationships are for illustrative purposes and should not be construed as limiting the invention.

  The chip mounting type integrated processing apparatus, chip-shaped container, and chip mounting type processing method according to the present invention are fields where processing of various solutions is required, for example, industrial fields, food fields, agricultural products, agricultural fields such as fish processing, It relates to all fields such as pharmaceutical field, hygiene, insurance, immunity, disease, genetic field, medical field, chemistry or biology field. The present invention is particularly effective when a series of processes using a large number of reagents and substances are continuously performed in a predetermined order.

1 is a perspective view showing a chip-mounted integrated processing apparatus according to a first embodiment of the present invention. It is a surface view which shows the nozzle arrangement pattern of the nozzle head which concerns on the 1st Embodiment of this invention. It is a perspective view which shows typically the nozzle head and chip | tip accommodating part which concern on the 1st Embodiment of this invention. It is a perspective view which shows typically the nozzle head and chip | tip accommodating part which concern on the 1st Embodiment of this invention. It is a perspective view which shows the chip | tip mounting | wearing type | mold integrated processing apparatus which mounted | wore the nozzle with the dispensing chip | tip concerning the 1st Embodiment of this invention. It is a partially cutaway figure which shows the dispensing tip which concerns on the 1st Embodiment of this invention. It is a partially cutaway view when a dispensing tip is attached to four types of nozzles belonging to each nozzle group according to the first embodiment of the present invention. It is a graph which shows the insertion amount and resistance force to the dispensing tip of the nozzle which concerns on the 1st Embodiment of this invention. It is a perspective view which shows typically the removal | desorption part provided in the nozzle head of the chip | tip mounting type | mold integrated processing apparatus which concerns on the 2nd Embodiment of this invention. It is a perspective view which shows typically the removal | desorption part provided on the stage of the chip | tip mounting type | mold integrated processing apparatus which concerns on the 3rd Embodiment of this invention. It is a perspective view which shows typically the removal | desorption part provided on the stage of the chip | tip mounting type | mold integrated processing apparatus which concerns on the 3rd Embodiment of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10,100,110 Chip mounting type | mold integrated processing apparatus 11,111 Desorption part 12 Nozzle head 16 Cylinder 14a, 14b, 14c, 14d Nozzle 20 Nozzle arrangement plate 42 Chip accommodating part (chip-shaped container accommodating part)
46 Dispensing tips (chip-shaped containers)
52a, 52b, 52c, 52d Rear end side outer peripheral protrusions 54a, 54b, 54c, 54d Front end side outer peripheral protrusions 60 Mounting opening 70 Rear end side inner peripheral cylindrical wall surface (inner peripheral wall surface)
72 Tip side inner peripheral cylindrical wall surface (inner peripheral wall surface)
64 mouth

Claims (10)

One or two or more nozzles having one or more outer peripheral projections projecting outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle, and two or more nozzles are arranged in a predetermined arrangement pattern The nozzle head, a suction / discharge mechanism that sucks and discharges gas through the nozzle, a mounting opening that can be attached to or attached to the nozzle, and a suction / discharge of the gas provided at the tip allow fluid to enter and exit. Two or more substantially cylindrical chip-like containers having possible mouth parts, and a chip-like container accommodating part that accommodates or accommodates the chip-like containers in a state that can be attached to the nozzles in the predetermined arrangement pattern; A moving means for relatively moving between the nozzle head and the tip-shaped container housing portion;
The two or more nozzles provided in the nozzle head are configured such that the outer peripheral protrusion provided in each nozzle is in close contact with or in contact with the inner peripheral wall surface of the mounting opening when the tip-shaped container is mounted. A chip-mounted integrated processing apparatus comprising a plurality of types of nozzle groups having different distances from a horizontal plane to at least one of the outer peripheral protrusions.   The two or more outer peripheral protrusions provided on the nozzle are formed such that the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side, and for mounting the tip-shaped container. The chip mounting type integrated processing apparatus according to claim 1, wherein an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion is formed in the opening corresponding to the outer peripheral protrusion.   The inner peripheral wall surface comprises a plurality of inner peripheral cylindrical wall surfaces provided to be spaced apart from each other corresponding to the outer peripheral protrusion, and each inner peripheral cylindrical wall surface is in close contact with or in contact with the outer peripheral protrusion. 3. The chip mounting type according to claim 1, wherein a taper taper surface is provided on a rear end side of the inner peripheral cylindrical wall surface so as to be connected to the inner peripheral cylindrical wall surface. Integrated processing equipment.   After the nozzles of the nozzle head reach a position where each nozzle of the nozzle head can be inserted into the mounting opening of the chip-shaped container, at least the structure of the nozzles belonging to the nozzle group and the number of nozzles belonging to each nozzle group The relative movement between the nozzle head and the tip-shaped container accommodating portion is such that each nozzle is inserted into the mounting opening based on the first to third embodiments. The chip mounting type integrated processing apparatus according to any one of the above.   The chip-like container attached to each nozzle has a desorption part for desorbing all at once, and the desorption part is larger than the maximum outer diameter or the maximum width of the horizontal cross section of the nozzle, but the maximum of the horizontal cross section of the chip-like container It has a desorption plate in which holes or gaps having a diameter or width smaller than the outer diameter or the maximum width are formed according to the predetermined arrangement pattern, and the desorption plate has a plate surface provided in parallel to the predetermined reference horizontal plane. The first to second aspects of the present invention are provided so as to be movable relative to the nozzle along the axial direction of the nozzle so that the axis of the nozzle passes through the hole or the gap. 5. The chip-mounted integrated processing container according to any one of 4 1 or 2 or more outer peripheral protrusions which protrude outwardly along the 1 or 2 or more closed outer periphery belt | band | zones mutually spaced apart in the axial direction, Comprising: And a mounting opening that can be mounted on a nozzle having a port, and a mouth that is provided at the tip of the container and through which the fluid can be entered and exited by suction and discharge of gas.
The mounting opening includes an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion of the nozzle when mounted on the nozzle, and a tapered connection that is provided on the rear end side of the inner peripheral wall surface and is connected to the inner peripheral wall surface. A chip-like container having a tapered surface.   The inner peripheral wall surface is provided corresponding to one or two or more outer peripheral protrusions protruding outward along two or more closed outer peripheral bands spaced from each other in the axial direction of the nozzle. The inner peripheral length of the inner peripheral cylindrical wall surface on the distal end side has a plurality of inner peripheral cylindrical wall surfaces that are in close contact with or in contact with the outer peripheral projections by mounting and spaced apart from each other in the axial direction of the container. Is formed shorter than the inner peripheral length of the inner peripheral cylindrical wall surface on the rear end side, and a tapered tapered surface is formed on the rear end side of the inner peripheral cylindrical wall surface. Chip-shaped container.   The outer peripheral protrusions of the two locations spaced apart from each other in the axial direction provided on the nozzle are formed such that the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side, The opening for mounting of the chip-shaped container has two inner peripheral cylindrical wall surfaces that are close to or in contact with the outer peripheral protrusion and are spaced apart from each other in the axial direction of the container. The inner peripheral length of the side inner peripheral cylindrical wall surface is shorter than the inner peripheral length of the rear end inner peripheral cylindrical wall surface, the rear end side, the rear end inner peripheral cylindrical wall surface and the front end side inner peripheral cylindrical wall surface A taper taper surface is formed between the side inner peripheral cylindrical wall surface, and the front end side inner peripheral cylindrical wall surface is in close contact with the outer peripheral protrusion on the front end side of the nozzle by being attached to the nozzle. The tip-like container according to claim 6, wherein the rear end side inner peripheral cylindrical wall surface is in contact with the rear end side outer peripheral protrusion. Nozzle having one or two or more outer peripheral projections projecting outward along two or more closed outer peripheral bands spaced apart from each other in one or the axial direction, and a nozzle in which two or more nozzles are arranged in a predetermined arrangement pattern A head, a suction / discharge mechanism that sucks and discharges gas through the nozzle, a mounting opening that can be attached to the nozzle, and a mouth portion that is provided at the tip and that allows fluid to enter and exit by suction and discharge of the gas. Two or more chip-shaped containers having a substantially cylindrical shape, a chip-shaped container storage section in which the chip-shaped containers are arrayed in the predetermined arrangement pattern and stored in a state that can be attached to the nozzles, and the nozzle head is formed in the chip shape And two or more nozzles provided in the nozzle head are arranged so that the outer peripheral projections of the nozzles are moved forward by the mounting of the tip-shaped container. Close or in contact with the inner peripheral wall surface of the fitting opening, with the distance of the outer peripheral protrusions from the predetermined reference horizontal surface until the peripheral protrusion comprises a plurality kinds of nozzle groups different from each other,
The nozzle head is moved to a position where each nozzle of the nozzle head can be inserted into the mounting opening of the tip-like container accommodated in the tip-like container accommodating portion, and the nozzle head is lowered, A chip mounting type integration processing method comprising a step of mounting a chip container on each nozzle of the nozzle head.   The chip-like container attached to each nozzle has a desorption part for desorbing all at once, and the desorption part is larger than the maximum outer diameter or the maximum width of the horizontal cross section of the nozzle, A desorption plate provided with holes or gaps having a diameter or width smaller than the maximum width in accordance with the predetermined arrangement pattern, the desorption plate having a plate surface provided in parallel to the predetermined reference horizontal plane; Alternatively, the step of detaching the tip-like container attached to the nozzle by moving the nozzle relative to the nozzle along the axial direction of the nozzle so that the axis of the nozzle passes through the gap. 10. The chip mounting type integration processing method according to claim 9, further comprising:

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