JPS649345A - Method of analyzing porphyrins - Google Patents

Method of analyzing porphyrins

Info

Publication number
JPS649345A
JPS649345A JP16491987A JP16491987A JPS649345A JP S649345 A JPS649345 A JP S649345A JP 16491987 A JP16491987 A JP 16491987A JP 16491987 A JP16491987 A JP 16491987A JP S649345 A JPS649345 A JP S649345A
Authority
JP
Japan
Prior art keywords
fluorescence
intensity
wavelength
znpp
grating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP16491987A
Other languages
Japanese (ja)
Inventor
Tetsuo Tamai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP16491987A priority Critical patent/JPS649345A/en
Publication of JPS649345A publication Critical patent/JPS649345A/en
Pending legal-status Critical Current

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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

PURPOSE:To enable the convenient and rapid quantification of ZnPP and PP in a biosample, by measuring the intensity of a fluorescence wavelength of 680nm so as to remove the background of ZnPP and PP for computation and the quantification. CONSTITUTION:A light of a wavelength of 410nm out of visible lights from a light source 1 is selected by a grating for an excitation wavelength and applied to a square cell 4 via a mirror 3a. Meanwhile, a fluorescence emitted in the direction at an angle of 90 deg. to the optical axis of the applied light is introduced into a grating 2b for a fluorescence wavelength via a mirror 3b, and the lights of wavelengths 589nm, 632nm and 680nm out of those separated in the grating 2b are received by photodiodes 5a-5c corresponding thereto. A fluorescence based on a component other than ZnPP is contained in the intensity of the fluorescence near 632nm, while a fluorescence based on a component other than PP is contained in the intensity of the fluorescence near 632nm. These fluorescences can be removed simply by executing subtraction with the intensity of the fluorescence near 680nm used as a background.
JP16491987A 1987-06-30 1987-06-30 Method of analyzing porphyrins Pending JPS649345A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16491987A JPS649345A (en) 1987-06-30 1987-06-30 Method of analyzing porphyrins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16491987A JPS649345A (en) 1987-06-30 1987-06-30 Method of analyzing porphyrins

Publications (1)

Publication Number Publication Date
JPS649345A true JPS649345A (en) 1989-01-12

Family

ID=15802344

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16491987A Pending JPS649345A (en) 1987-06-30 1987-06-30 Method of analyzing porphyrins

Country Status (1)

Country Link
JP (1) JPS649345A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4963605A (en) * 1985-11-08 1990-10-16 Sumitomo Chemical Company, Limited Stabilized polyolefin composition
JP2005030839A (en) * 2003-07-09 2005-02-03 Dkk Toa Corp Water quality measuring method and device
JP2006284298A (en) * 2005-03-31 2006-10-19 Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai Diagnostic method of tumor of colon
JP2009180745A (en) * 2009-05-18 2009-08-13 Hitachi High-Technologies Corp Fluorescence detection method, detecting device, and fluorescence detection program
JP2010240078A (en) * 2009-04-02 2010-10-28 Sbi Alapromo Co Ltd Automatic tumor discriminator and automatic discrimination method for tumor region
JP2011209068A (en) * 2010-03-29 2011-10-20 Institute Of National Colleges Of Technology Japan Method for preparing biosample
WO2013002350A1 (en) * 2011-06-29 2013-01-03 京都府公立大学法人 Tumor site identification device and method
CN103175818A (en) * 2013-03-19 2013-06-26 桂林理工大学 Method for detecting melamine
CN104597015A (en) * 2015-01-09 2015-05-06 东南大学 Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4963605A (en) * 1985-11-08 1990-10-16 Sumitomo Chemical Company, Limited Stabilized polyolefin composition
JP2005030839A (en) * 2003-07-09 2005-02-03 Dkk Toa Corp Water quality measuring method and device
JP2006284298A (en) * 2005-03-31 2006-10-19 Hamamatsu Kagaku Gijutsu Kenkyu Shinkokai Diagnostic method of tumor of colon
JP2010240078A (en) * 2009-04-02 2010-10-28 Sbi Alapromo Co Ltd Automatic tumor discriminator and automatic discrimination method for tumor region
JP2009180745A (en) * 2009-05-18 2009-08-13 Hitachi High-Technologies Corp Fluorescence detection method, detecting device, and fluorescence detection program
JP2011209068A (en) * 2010-03-29 2011-10-20 Institute Of National Colleges Of Technology Japan Method for preparing biosample
WO2013002350A1 (en) * 2011-06-29 2013-01-03 京都府公立大学法人 Tumor site identification device and method
JPWO2013002350A1 (en) * 2011-06-29 2015-02-23 京都府公立大学法人 Tumor site identification device and identification method
US9241636B2 (en) 2011-06-29 2016-01-26 Kyoto Prefectural Public University Corporation Tumor site or parathyroid gland identification device and method
CN103175818A (en) * 2013-03-19 2013-06-26 桂林理工大学 Method for detecting melamine
CN104597015A (en) * 2015-01-09 2015-05-06 东南大学 Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe

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