JPS6486871A - Method for separating and purifying recombinant protein - Google Patents
Method for separating and purifying recombinant proteinInfo
- Publication number
- JPS6486871A JPS6486871A JP24701787A JP24701787A JPS6486871A JP S6486871 A JPS6486871 A JP S6486871A JP 24701787 A JP24701787 A JP 24701787A JP 24701787 A JP24701787 A JP 24701787A JP S6486871 A JPS6486871 A JP S6486871A
- Authority
- JP
- Japan
- Prior art keywords
- microbial cells
- chromatography
- escherichia coli
- bacillus subtilis
- recombinant plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 230000000813 microbial effect Effects 0.000 abstract 3
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 2
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 229960002385 streptomycin sulfate Drugs 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 101900343733 Bacillus subtilis Dihydrofolate reductase Proteins 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000004440 column chromatography Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000001641 gel filtration chromatography Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6486871A true JPS6486871A (en) | 1989-03-31 |
| JPH0333315B2 JPH0333315B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1991-05-16 |
Family
ID=17157159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24701787A Granted JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6486871A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1318546A4 (en) | 2000-07-19 | 2007-08-08 | Shindo Company Ltd | SEMICONDUCTOR COMPONENT |
-
1987
- 1987-09-30 JP JP24701787A patent/JPS6486871A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0333315B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1991-05-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |