JPS6486868A - Column for hydrolyzing protein - Google Patents

Column for hydrolyzing protein

Info

Publication number
JPS6486868A
JPS6486868A JP24668287A JP24668287A JPS6486868A JP S6486868 A JPS6486868 A JP S6486868A JP 24668287 A JP24668287 A JP 24668287A JP 24668287 A JP24668287 A JP 24668287A JP S6486868 A JPS6486868 A JP S6486868A
Authority
JP
Japan
Prior art keywords
column
packing
liquid chromatography
resultant mixture
proteolytic enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP24668287A
Other languages
Japanese (ja)
Inventor
Teruhisa Ueda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP24668287A priority Critical patent/JPS6486868A/en
Publication of JPS6486868A publication Critical patent/JPS6486868A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE:To obtain the titled column, capable of automatically carrying out continuous hydrolysis and separation of proteins in using for liquid chromatography, by mixing a packing immobilizing a proteolytic enzyme with a packing for separating peptide fragments and filling the resultant mixture in a single column. CONSTITUTION:(A) A proteolytic enzyme, such as trypsin, is immobilized in a packing by, e.g. a glutaraldehyde method, and mixed with (B) a packing having functional groups (e.g. anionic exchange reactive groups) capable of separating peptide fragments of hydrolyzed proteins and the resultant mixture is filled in a column to afford the aimed column for liquid chromatography.
JP24668287A 1987-09-30 1987-09-30 Column for hydrolyzing protein Pending JPS6486868A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP24668287A JPS6486868A (en) 1987-09-30 1987-09-30 Column for hydrolyzing protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24668287A JPS6486868A (en) 1987-09-30 1987-09-30 Column for hydrolyzing protein

Publications (1)

Publication Number Publication Date
JPS6486868A true JPS6486868A (en) 1989-03-31

Family

ID=17152050

Family Applications (1)

Application Number Title Priority Date Filing Date
JP24668287A Pending JPS6486868A (en) 1987-09-30 1987-09-30 Column for hydrolyzing protein

Country Status (1)

Country Link
JP (1) JPS6486868A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012526288A (en) * 2009-05-08 2012-10-25 サイノファーム タイワン リミテッド Methods for analyzing peptide mixtures
JP2016176947A (en) * 2016-04-07 2016-10-06 株式会社島津製作所 Methods of preparing and analyzing peptide fragments
JP2017515129A (en) * 2014-05-02 2017-06-08 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ Functionalized support materials and methods of making and using functionalized support materials
US10695744B2 (en) 2015-06-05 2020-06-30 W. R. Grace & Co.-Conn. Adsorbent biprocessing clarification agents and methods of making and using the same
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012526288A (en) * 2009-05-08 2012-10-25 サイノファーム タイワン リミテッド Methods for analyzing peptide mixtures
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices
JP2017515129A (en) * 2014-05-02 2017-06-08 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ Functionalized support materials and methods of making and using functionalized support materials
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
US10695744B2 (en) 2015-06-05 2020-06-30 W. R. Grace & Co.-Conn. Adsorbent biprocessing clarification agents and methods of making and using the same
JP2016176947A (en) * 2016-04-07 2016-10-06 株式会社島津製作所 Methods of preparing and analyzing peptide fragments

Similar Documents

Publication Publication Date Title
AU1056188A (en) Immunoaffinity purification system
Kopeyan et al. Disulfide bonds of toxin II of the scorpion Androctonus australis Hector
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
ATE171216T1 (en) EXPRESSION AND PURIFICATION OF RECOMBINANT SOLUBLE TISSUE FACTOR
GB2197324B (en) Process for the production of a polypeptide
EP0799317B8 (en) Method for secreting thrombopoietin polypeptides
IE900749L (en) A yeast processing system
EP0145390A3 (en) A method for producing non-glycosylated human interleukin-2 protein
JPS6486868A (en) Column for hydrolyzing protein
SE7909488L (en) HYDRAINIC AMINO ACID PREPARATION AND PROCEDURE FOR THE PREPARATION OF HYDRAINIC AMINO ACID PREPARATION FROM HOME PROTEINS
AU569896B2 (en) Process for the dissolution of peptides and proteins
MY109153A (en) Enzymatic hydrolysis
Gross et al. The heterogeneity of the S peptide of bovine pancreatic ribonuclease A
AU7166891A (en) Method of purifying recombinant polypeptides
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
Lazo et al. Modification of fructose bisphosphatase by a proteolytic enzyme from rat liver lysosomes
DE69318495D1 (en) Process for the purification of the big endothelin protein
PL314801A1 (en) Method of purifying vitamin-k-dependent proteins by using a membrane chromatography process
CA2190802A1 (en) A method of preparing and recovering proteins
Shenolikar et al. Purification and partial characterization of a thiol proteinase from the thermophilic fungus Humicola lanuginosa
EP0270055A3 (en) Synthetic peptide compounds producing antibodies binding to human ldh-c4
IE821249L (en) Peptides
IE830260L (en) Process for the preparation of protein for hydrolysis.
Fielbal et al. Isolation of neurosecretory granules containing vasopressin and MSEL-neurophysin from guinea pig neurointermediate pituitary
NZ209588A (en) Production of human immune interferon and pharmaceutical composition