JPS6480290A - Dna sequence, vector and production of lipase - Google Patents

Dna sequence, vector and production of lipase

Info

Publication number
JPS6480290A
JPS6480290A JP10603587A JP10603587A JPS6480290A JP S6480290 A JPS6480290 A JP S6480290A JP 10603587 A JP10603587 A JP 10603587A JP 10603587 A JP10603587 A JP 10603587A JP S6480290 A JPS6480290 A JP S6480290A
Authority
JP
Japan
Prior art keywords
lipase
plasmid
gene
mold
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10603587A
Other languages
Japanese (ja)
Other versions
JP2540540B2 (en
Inventor
Wataru Kugimiya
Yasuo Otani
Yukio Hashimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuji Oil Co Ltd
Original Assignee
Fuji Oil Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Oil Co Ltd filed Critical Fuji Oil Co Ltd
Priority to JP62106035A priority Critical patent/JP2540540B2/en
Publication of JPS6480290A publication Critical patent/JPS6480290A/en
Application granted granted Critical
Publication of JP2540540B2 publication Critical patent/JP2540540B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To efficiently produce a lipase, by cloning 1,3-specific lipase gene of a mold, inserting the gene into a pUC based plasmid having a lactose promoter and introducing the obtained plasmid into Escherichia coil. CONSTITUTION:A cDNA bank is prepared from an mRNA of Rhizopus.niveus and an oligonucleotide partially corresponding to the amino acid sequence of a lipase of purified mold is used as a probe to carry out plaque hybridization and afford a hybridizable clone lambdaRL106B. A DNA fragment containing cDNA of the above-mentioned lambdaRL106B inserted thereinto is then inserted into an ECoRI part of pUC8 to provide a plasmid pRL106B, which is subsequently introduced into Escherichia coli JM109. The resultant transformant strain is then cultivated in a culture medium to produce the aimed lipase.
JP62106035A 1987-04-28 1987-04-28 Lipase gene and recombinant DNA Expired - Lifetime JP2540540B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62106035A JP2540540B2 (en) 1987-04-28 1987-04-28 Lipase gene and recombinant DNA

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62106035A JP2540540B2 (en) 1987-04-28 1987-04-28 Lipase gene and recombinant DNA

Publications (2)

Publication Number Publication Date
JPS6480290A true JPS6480290A (en) 1989-03-27
JP2540540B2 JP2540540B2 (en) 1996-10-02

Family

ID=14423383

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62106035A Expired - Lifetime JP2540540B2 (en) 1987-04-28 1987-04-28 Lipase gene and recombinant DNA

Country Status (1)

Country Link
JP (1) JP2540540B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03290186A (en) * 1990-04-04 1991-12-19 Fuji Oil Co Ltd Production of lipase
US5219753A (en) * 1989-06-22 1993-06-15 The United States Of America As Represented By The Secretary Of Agriculture Genetically engineered microorganisms containing a gene segment coding for a lipase from Rhizopus delemar

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AGRIC BIOL CHEM=1983 *
BIOCHEM BIOPHYS RES COMMUN=1986 *
EUR J BIOCHEM=1986 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219753A (en) * 1989-06-22 1993-06-15 The United States Of America As Represented By The Secretary Of Agriculture Genetically engineered microorganisms containing a gene segment coding for a lipase from Rhizopus delemar
JPH03290186A (en) * 1990-04-04 1991-12-19 Fuji Oil Co Ltd Production of lipase

Also Published As

Publication number Publication date
JP2540540B2 (en) 1996-10-02

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