JPS6480280A - Preparation of bacterial cell resistant to bacteriophage - Google Patents

Preparation of bacterial cell resistant to bacteriophage

Info

Publication number
JPS6480280A
JPS6480280A JP62234746A JP23474687A JPS6480280A JP S6480280 A JPS6480280 A JP S6480280A JP 62234746 A JP62234746 A JP 62234746A JP 23474687 A JP23474687 A JP 23474687A JP S6480280 A JPS6480280 A JP S6480280A
Authority
JP
Japan
Prior art keywords
strain
phage
resistant
omt
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62234746A
Other languages
Japanese (ja)
Inventor
Shoichiro Miyahara
Masaru Yasui
Masashi Hashimukai
Kazunari Nitta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Priority to JP62234746A priority Critical patent/JPS6480280A/en
Publication of JPS6480280A publication Critical patent/JPS6480280A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain a phage-resistant strain without accompanying the deterioration of activity, by converting a host strain free from a plasmid carrying the objective gene into a strain resistant to bacteriophage and introducing a plasmid carrying the objective gene into the mutant. CONSTITUTION:Plasmid pMTC-1 containing a gene coding tryptophan synthase is treated with a calcium chloride solution at a low temperature using E.coli k-12HB101 strain as a host and is introduced into the host cell to obtain OMT-2050 strain. Said OMT-2050 strain is cultured in a medium containing bacto-trypton, etc., by flask culture to generate a bacteriolytic phage. Purified phage OS1 is separated from the lysed solution. E.coli k-12HB101 strain is made to be resistant to phage with said phage liquid and the obtained resistant strain is cultured, a competent cell is prepared and a plasmid pMTC-1 is introduced to obtain a transformant OMT-2052 (FERM P-9528).
JP62234746A 1987-09-21 1987-09-21 Preparation of bacterial cell resistant to bacteriophage Pending JPS6480280A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62234746A JPS6480280A (en) 1987-09-21 1987-09-21 Preparation of bacterial cell resistant to bacteriophage

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62234746A JPS6480280A (en) 1987-09-21 1987-09-21 Preparation of bacterial cell resistant to bacteriophage

Publications (1)

Publication Number Publication Date
JPS6480280A true JPS6480280A (en) 1989-03-27

Family

ID=16975707

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62234746A Pending JPS6480280A (en) 1987-09-21 1987-09-21 Preparation of bacterial cell resistant to bacteriophage

Country Status (1)

Country Link
JP (1) JPS6480280A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176868A (en) * 1991-01-24 1993-01-05 Prince Manufacturing, Inc. Long fiber reinforced thermoplastic frame especially for a tennis racquet
WO2005056776A1 (en) * 2003-12-15 2005-06-23 Cj Corp. E.coli mutant containing mutant genes related with tryptophan biosynthesis and production method of tryptophan by using the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176868A (en) * 1991-01-24 1993-01-05 Prince Manufacturing, Inc. Long fiber reinforced thermoplastic frame especially for a tennis racquet
WO2005056776A1 (en) * 2003-12-15 2005-06-23 Cj Corp. E.coli mutant containing mutant genes related with tryptophan biosynthesis and production method of tryptophan by using the same
KR101142885B1 (en) * 2003-12-15 2012-05-10 씨제이제일제당 (주) E.coli mutant strain containing mutant genes related with Tryptophan biosynthesis and Production method of Tryptophan by using the same

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