JPS6479654A - Polyamine analysis - Google Patents

Polyamine analysis

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Publication number
JPS6479654A
JPS6479654A JP23800887A JP23800887A JPS6479654A JP S6479654 A JPS6479654 A JP S6479654A JP 23800887 A JP23800887 A JP 23800887A JP 23800887 A JP23800887 A JP 23800887A JP S6479654 A JPS6479654 A JP S6479654A
Authority
JP
Japan
Prior art keywords
polyamine
methylalcohol
mobile phase
sample
mixed solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23800887A
Other languages
Japanese (ja)
Inventor
Keiji Gamo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP23800887A priority Critical patent/JPS6479654A/en
Publication of JPS6479654A publication Critical patent/JPS6479654A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To analyze polyamine in a short time, by employing a mixed solution of hexane sodium sulfonate and sodium chlorate and methylalcohol as mobile phase in an anti-phase distribution chromatography. CONSTITUTION:A mixed solution of 5-10mM of hexane sodium sulfonate and 0.5-0.1M of sodium chlorate is sent as mobile phase A and a methylalcohol solution as mobile phase B and a sample is injected with an injection section 3. Then, the sample is distributed by an anti-phase distribution chromatography column 4. Then, the concentration of methylalcohol is increased from 3% to 41% to perform a 2-liquid gradient elution. Then, a reaction reagent 6 is added to detect 5 fluorescence and a data processing 11 is performed whereby putrescine, spermidine, cadaverine and the like as polyamine are eluted. The use of the above-mentioned eluate can simplify the composition of the eluate thereby enabling highly accurate analysis of polyamine of various types.
JP23800887A 1987-09-22 1987-09-22 Polyamine analysis Pending JPS6479654A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23800887A JPS6479654A (en) 1987-09-22 1987-09-22 Polyamine analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23800887A JPS6479654A (en) 1987-09-22 1987-09-22 Polyamine analysis

Publications (1)

Publication Number Publication Date
JPS6479654A true JPS6479654A (en) 1989-03-24

Family

ID=17023770

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23800887A Pending JPS6479654A (en) 1987-09-22 1987-09-22 Polyamine analysis

Country Status (1)

Country Link
JP (1) JPS6479654A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108872414A (en) * 2018-04-24 2018-11-23 许昌学院 A kind of method that LC-MS/MS measures the cadaverine in soy sauce

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108872414A (en) * 2018-04-24 2018-11-23 许昌学院 A kind of method that LC-MS/MS measures the cadaverine in soy sauce

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