JPS6479654A - Polyamine analysis - Google Patents
Polyamine analysisInfo
- Publication number
- JPS6479654A JPS6479654A JP23800887A JP23800887A JPS6479654A JP S6479654 A JPS6479654 A JP S6479654A JP 23800887 A JP23800887 A JP 23800887A JP 23800887 A JP23800887 A JP 23800887A JP S6479654 A JPS6479654 A JP S6479654A
- Authority
- JP
- Japan
- Prior art keywords
- polyamine
- methylalcohol
- mobile phase
- sample
- mixed solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE:To analyze polyamine in a short time, by employing a mixed solution of hexane sodium sulfonate and sodium chlorate and methylalcohol as mobile phase in an anti-phase distribution chromatography. CONSTITUTION:A mixed solution of 5-10mM of hexane sodium sulfonate and 0.5-0.1M of sodium chlorate is sent as mobile phase A and a methylalcohol solution as mobile phase B and a sample is injected with an injection section 3. Then, the sample is distributed by an anti-phase distribution chromatography column 4. Then, the concentration of methylalcohol is increased from 3% to 41% to perform a 2-liquid gradient elution. Then, a reaction reagent 6 is added to detect 5 fluorescence and a data processing 11 is performed whereby putrescine, spermidine, cadaverine and the like as polyamine are eluted. The use of the above-mentioned eluate can simplify the composition of the eluate thereby enabling highly accurate analysis of polyamine of various types.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23800887A JPS6479654A (en) | 1987-09-22 | 1987-09-22 | Polyamine analysis |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP23800887A JPS6479654A (en) | 1987-09-22 | 1987-09-22 | Polyamine analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6479654A true JPS6479654A (en) | 1989-03-24 |
Family
ID=17023770
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP23800887A Pending JPS6479654A (en) | 1987-09-22 | 1987-09-22 | Polyamine analysis |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6479654A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108872414A (en) * | 2018-04-24 | 2018-11-23 | 许昌学院 | A kind of method that LC-MS/MS measures the cadaverine in soy sauce |
-
1987
- 1987-09-22 JP JP23800887A patent/JPS6479654A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108872414A (en) * | 2018-04-24 | 2018-11-23 | 许昌学院 | A kind of method that LC-MS/MS measures the cadaverine in soy sauce |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Duerre et al. | Preparation of L-homocysteine from L-homocysteine thiolactone | |
Cherrah et al. | Study of deuterium isotope effects on protein binding by gas chromatography/mass spectrometry. Caffeine and deuterated isotopomers | |
Nolan et al. | Isotope techniques for studying the dynamics of nitrogen metabolism in ruminants | |
Martin et al. | Ribonucleotide composition of the genetic code | |
ES8402422A1 (en) | Detection and differentiation of molecules naturally containing deuterium and application among others for determining the addition of sugar to wines. | |
Schapira et al. | The ambiguities in the rabbit hemoglobin: evidence for a messenger RNA translated specifically into hemoglobin | |
Steinberg et al. | Preparation of tritiated proteins by the Wilzbach method | |
Bellino et al. | Estrogen synthetase in choriocarcinoma cell culture. Stimulation by dibutyryl cyclic adenosine monophosphate and theophylline | |
GB1296184A (en) | ||
JPS6479654A (en) | Polyamine analysis | |
Spiro | Uronic acid analysis by automated anion exchange chromatography | |
GB1349491A (en) | Fluorimetric assay of primary amino groups | |
Stipanuk et al. | Cysteinesulfinic acid, hypotaurine, and taurine: reversed-phase high-performance liquid chromatography | |
Belitsina et al. | Ribosomal Translocation Assayed by the Matrix‐Bound Poly (uridylic acid) Column Technique: Effects of Different Magnesium Concentrations | |
Natelson et al. | On the biosynthesis of guanidinosuccinate. | |
Brodasky | Reproducibility of Rf and Correlation of Chromatic Patterns on Paper and Thin Layer Plates | |
NO177809C (en) | Process for rapid mixing of small volumes to enhance biological reactions | |
Zhuravlev et al. | Determination of the samarium-neodymium ratio for the purpose of geochronology(Opredelenie samarii-neodimovogo otnosheniia dlia tselei geokhronologii) | |
JPS57194351A (en) | Bile acid analysing method | |
JPS57135355A (en) | Method and device for analyzing amino acid | |
Epstein et al. | DSS as an activator of carboxypeptidase A | |
RU94032808A (en) | Process of determination of acetophenon in air | |
Seely et al. | Determination of several ammonia or amino acid samples on one chromatogram with an amino acid analyzer | |
Nekvasilová et al. | Effect of peptidic groups isolated from enzymic casein hydrolysate on growth and toxinogenesis of Clostridium welchii (perfringens) | |
GB1492892A (en) | Method and apparatus for performing a separating analysis |