JPS6471477A - Microbial strain capable of producing l-amino acid - Google Patents
Microbial strain capable of producing l-amino acidInfo
- Publication number
- JPS6471477A JPS6471477A JP22788887A JP22788887A JPS6471477A JP S6471477 A JPS6471477 A JP S6471477A JP 22788887 A JP22788887 A JP 22788887A JP 22788887 A JP22788887 A JP 22788887A JP S6471477 A JPS6471477 A JP S6471477A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- amino acid
- reaction
- carbamoylamino
- alcaligenes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To produce an L-amino acid in high efficiency from N-carbamoylamino acid having high concentration, by using Alcaligenes NS 540 stain and performing a reaction in an alkaline region in which the solubility of N- carbamoylamino acid increases. CONSTITUTION:The objective Alcaligenes sp NS 520 (FERM P-9544) is a Gram- negative bacillus having locomotivity, positive to catalase, positive to oxidase, free from acid fastness, free from sporulation, having aerobic nature, non- fermentative in OF test and negative to VP reaction and arginine decomposition activity, etc. An L-amino acid is produced by culturing said NS 540 strain in a medium containing carbon source, nitrogen source, inorganic salt, etc., at pH5-10 and 10-35 deg.C for 1-3 days and reacting N-carbamoylamino acid of 1-20% concentration with the obtained cultured product as an enzyme source at pH8-10 and 15-50 deg.C. The reaction liquid is preferably added with 0.1-10mM of manganese sulfate, cobalt chloride, etc.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22788887A JPS6471477A (en) | 1987-09-11 | 1987-09-11 | Microbial strain capable of producing l-amino acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP22788887A JPS6471477A (en) | 1987-09-11 | 1987-09-11 | Microbial strain capable of producing l-amino acid |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6471477A true JPS6471477A (en) | 1989-03-16 |
Family
ID=16867903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP22788887A Pending JPS6471477A (en) | 1987-09-11 | 1987-09-11 | Microbial strain capable of producing l-amino acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6471477A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5863785A (en) * | 1992-10-05 | 1999-01-26 | Kanegafuchi Chemical Industry Co., Ltd. | Decarbamylase isolated from comamonas or blastobacter |
-
1987
- 1987-09-11 JP JP22788887A patent/JPS6471477A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5863785A (en) * | 1992-10-05 | 1999-01-26 | Kanegafuchi Chemical Industry Co., Ltd. | Decarbamylase isolated from comamonas or blastobacter |
US5902736A (en) * | 1992-10-05 | 1999-05-11 | Kanegafuchi Chemical Industry Co., Ltd. | Process for the production of D-α-amino acids by hydrolysis of the corresponding N-carbamyl derivative |
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