JPS6445391A - Novel method for measuring alpha-amylase activity - Google Patents
Novel method for measuring alpha-amylase activityInfo
- Publication number
- JPS6445391A JPS6445391A JP20112187A JP20112187A JPS6445391A JP S6445391 A JPS6445391 A JP S6445391A JP 20112187 A JP20112187 A JP 20112187A JP 20112187 A JP20112187 A JP 20112187A JP S6445391 A JPS6445391 A JP S6445391A
- Authority
- JP
- Japan
- Prior art keywords
- halogen
- formula
- amylase activity
- nitro
- lower alkoxy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
NEW MATERIAL:A compound expressed by formula I [R<1> is formula II-IV (R<2>-R<5> are H, lower alkyl, lower alkoxy, nitro, carboxyl, sulfonic acid, halogen, etc.; R<6> is H, lower alkoxy, halogen or nitro; R<7> is H, methyl or trifluoromethyl; R<8> is H or halogen)]. USE:A substrate for measuring alpha-amylase activity applicable also to dry quantitative methods, since measurement can be made by colorimetry. PREPARATION:An oligosaccharide derivative expressed by formula V is reacted with acetals, such as benzaldehyde dimethyl acetal, to form a cyclic acetal group between the 4- and the 6-positions of nonreducing terminal glucose. The resultant cyclic acetalized substance is then acylated to modify other OH groups. The modified compound is subsequently subjected to a reduction step and then deacylated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20112187A JPH0676430B2 (en) | 1987-08-12 | 1987-08-12 | New method for measuring α-amylase activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20112187A JPH0676430B2 (en) | 1987-08-12 | 1987-08-12 | New method for measuring α-amylase activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6445391A true JPS6445391A (en) | 1989-02-17 |
| JPH0676430B2 JPH0676430B2 (en) | 1994-09-28 |
Family
ID=16435758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20112187A Expired - Lifetime JPH0676430B2 (en) | 1987-08-12 | 1987-08-12 | New method for measuring α-amylase activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0676430B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0391496A (en) * | 1989-09-04 | 1991-04-17 | Boehringer Mannheim Gmbh | Method and reagent for specific measurement of pancreas alpha amylase |
-
1987
- 1987-08-12 JP JP20112187A patent/JPH0676430B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0391496A (en) * | 1989-09-04 | 1991-04-17 | Boehringer Mannheim Gmbh | Method and reagent for specific measurement of pancreas alpha amylase |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0676430B2 (en) | 1994-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |