JPS6443502A - Fractionation of hyaluronic acid - Google Patents
Fractionation of hyaluronic acidInfo
- Publication number
- JPS6443502A JPS6443502A JP19908787A JP19908787A JPS6443502A JP S6443502 A JPS6443502 A JP S6443502A JP 19908787 A JP19908787 A JP 19908787A JP 19908787 A JP19908787 A JP 19908787A JP S6443502 A JPS6443502 A JP S6443502A
- Authority
- JP
- Japan
- Prior art keywords
- salt
- cpd
- hyaluronic acid
- org
- fractionated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
PURPOSE:To fractionate hyaluronic acid or a salt thereof in a small width of MW distribution and a desired size, by processing said acid or said salt into a conjugated cpd. with a cationic surface active agent and applying the cpd. to a molecular sieve. CONSTITUTION:Hyaluronic acid or a salt thereof is processed into a conjugated cpd. with a cationic surface active agent. The cpd., dissolved in a water-soluble org. solvent, is fractionated by means of chromatography, and is subsequently dissociated to give a fractionated hyaluronic acid or salt thereof. The cpd. can be obtd. by adding dropwise a cationic surface active agent, e.g., cetyltrimethylammonium halide or cetylpyridinium halide, to an aq. soln. of hyaluronic acid or a salt thereof and mixing them. The chromatography is carried out using, for example, a gel filtration or gel permeation carrier having a desired fractionation MW range and having been washed with an org. solvent of the same kind as the one dissolving the composite. The dissociation of the conjugate is carried out by adding to the fractionated soln. an inorg. salt of an org. acid soluble in the org. solvent used for the fractionation (e.g., sodium acetate).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62199087A JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62199087A JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6443502A true JPS6443502A (en) | 1989-02-15 |
JPH0813847B2 JPH0813847B2 (en) | 1996-02-14 |
Family
ID=16401886
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62199087A Expired - Lifetime JPH0813847B2 (en) | 1987-08-11 | 1987-08-11 | Fractionation method of hyaluronic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0813847B2 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05508183A (en) * | 1991-04-19 | 1993-11-18 | フィディーア・ソシエタ・ペル・アチオニ | Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology |
US6946551B2 (en) | 2003-03-12 | 2005-09-20 | New Life Resources, Llc | Preparation of hyaluronic acid from eggshell membrane |
US8580315B2 (en) | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
JP2014101470A (en) * | 2012-11-22 | 2014-06-05 | Sumitomo Bakelite Co Ltd | Sugar chain purification method |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS609042A (en) * | 1983-06-09 | 1985-01-18 | ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン | Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same |
JPS60133894A (en) * | 1983-11-25 | 1985-07-17 | マイルス・ラボラトリース・インコーポレーテツド | Hyaruronic acid and its production |
-
1987
- 1987-08-11 JP JP62199087A patent/JPH0813847B2/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS609042A (en) * | 1983-06-09 | 1985-01-18 | ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン | Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same |
JPS60133894A (en) * | 1983-11-25 | 1985-07-17 | マイルス・ラボラトリース・インコーポレーテツド | Hyaruronic acid and its production |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05508183A (en) * | 1991-04-19 | 1993-11-18 | フィディーア・ソシエタ・ペル・アチオニ | Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology |
US6946551B2 (en) | 2003-03-12 | 2005-09-20 | New Life Resources, Llc | Preparation of hyaluronic acid from eggshell membrane |
US8580315B2 (en) | 2004-03-10 | 2013-11-12 | Esm Technologies, Llc | Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations |
JP2014101470A (en) * | 2012-11-22 | 2014-06-05 | Sumitomo Bakelite Co Ltd | Sugar chain purification method |
Also Published As
Publication number | Publication date |
---|---|
JPH0813847B2 (en) | 1996-02-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
PL289487A1 (en) | Method of producing new aminophenol derivatives | |
DE59804066D1 (en) | Triazolinthion-phosphorsäure-derivate | |
BR9907679A (en) | Sterile therapeutic peptide complex linked to a polysaccharide | |
HUT50767A (en) | Process for producing new 1,3,4,5-tetrahydro-benz/c,d/indole derivatives and pharmaceutical compositions comprising such compounds | |
IL113450A (en) | Process for the preparation of high solubility multicomponent inclusion complexes consisting of an acidic drug a cyclodextrin and a base and the complexes produced thereby | |
ES2169429T3 (en) | Glycoconjugates of modified derivatives of CAMPTOTECHINE (LINK IN 20-O). | |
RU95105977A (en) | INCLUSION COMPLEXES OF SALTS OF NIMESUlIDE WITH CYCLODEXTRINES, METHOD FOR THEIR PRODUCTION, PHARMACEUTICAL COMPOSITIONS ON THEIR BASE AND METHOD OF TREATMENT | |
CA1034128A (en) | Amidocarbonylthiobarbituric acid derivatives, process for their preparation and their use as insecticides, and plant protection agents | |
KR950700310A (en) | Heterotricyclic derivatives, methods for their preparation and pharmaceutical compositions containing them (HETEROTRICYCLIC DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM) | |
JPS6443502A (en) | Fractionation of hyaluronic acid | |
CA2163197A1 (en) | Tri(platinum) complexes | |
HU177823B (en) | Rodenticide composition containing n-alkyl-2,4-dinitro-6-trifluoromethyl-diphenyl-amine derivatives and process for producing the active agents | |
EP0214017A3 (en) | Use of n,n,-dimethylbiguanid p-chorophenoxyacetate in the treatment of neuropathies and nerval degeneration | |
NL7601378A (en) | PROCESS FOR THE PREPARATION OF UNSATURATED POLYESTERAMIDS AND ARTICLES MANUFACTURED USING THE UNSATURIFIED POLYESTERAMIDES PREPARED IN THIS WAY. | |
DE3477924D1 (en) | Benzenesulphonyl lactams, process for their preparation and their use as active ingredients in pharmaceutical compounds | |
TR199902775T2 (en) | (S) -Camptothecin glycoconjugeleri. | |
JPS54105223A (en) | Coating agent for solid medicine | |
EP0000741A3 (en) | Indolacetoxy acetylamino acid derivatives, process for their preparation and their use as drugs | |
DE69012924D1 (en) | Azole-substituted cycloalkanol derivatives, process for their preparation and use in agriculture and horticulture. | |
HU9201799D0 (en) | New 2-amino-5-ciano-1,4-dihydropiridine-derivatives, process for their use and their use as medicaments | |
GR3024631T3 (en) | Dyes, their preparation and use in the coloration of substrates. | |
ATE32897T1 (en) | PYRAZOLO(3,4,5-KL)ACRIDINDERIVATE, PHARMACEUTICAL PREPARATIONS THEY CONTAIN AND PROCESS FOR THEIR MANUFACTURE. | |
ZA898775B (en) | Cyclopropyl-substituted azolylmethylcarbinols,processes for their preparation and their use as medicaments | |
DE69618496D1 (en) | Process for the derivatization of polyglucosamines | |
HRP960063B1 (en) | Substituted benzenesulfonylureas and -thioureas, process for their preparation and their use in the production of pharmaceutical preparations as well as pharmaceutical preparations containing them |