JPS6443502A - Fractionation of hyaluronic acid - Google Patents

Fractionation of hyaluronic acid

Info

Publication number
JPS6443502A
JPS6443502A JP19908787A JP19908787A JPS6443502A JP S6443502 A JPS6443502 A JP S6443502A JP 19908787 A JP19908787 A JP 19908787A JP 19908787 A JP19908787 A JP 19908787A JP S6443502 A JPS6443502 A JP S6443502A
Authority
JP
Japan
Prior art keywords
salt
cpd
hyaluronic acid
org
fractionated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP19908787A
Other languages
Japanese (ja)
Other versions
JPH0813847B2 (en
Inventor
Zenji Ogawa
Mamoru Seki
Yoshio Mogi
Hiroaki Nishiyama
Yoshimi Inoue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Kayaku Co Ltd
Original Assignee
Nippon Kayaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Kayaku Co Ltd filed Critical Nippon Kayaku Co Ltd
Priority to JP62199087A priority Critical patent/JPH0813847B2/en
Publication of JPS6443502A publication Critical patent/JPS6443502A/en
Publication of JPH0813847B2 publication Critical patent/JPH0813847B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Landscapes

  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

PURPOSE:To fractionate hyaluronic acid or a salt thereof in a small width of MW distribution and a desired size, by processing said acid or said salt into a conjugated cpd. with a cationic surface active agent and applying the cpd. to a molecular sieve. CONSTITUTION:Hyaluronic acid or a salt thereof is processed into a conjugated cpd. with a cationic surface active agent. The cpd., dissolved in a water-soluble org. solvent, is fractionated by means of chromatography, and is subsequently dissociated to give a fractionated hyaluronic acid or salt thereof. The cpd. can be obtd. by adding dropwise a cationic surface active agent, e.g., cetyltrimethylammonium halide or cetylpyridinium halide, to an aq. soln. of hyaluronic acid or a salt thereof and mixing them. The chromatography is carried out using, for example, a gel filtration or gel permeation carrier having a desired fractionation MW range and having been washed with an org. solvent of the same kind as the one dissolving the composite. The dissociation of the conjugate is carried out by adding to the fractionated soln. an inorg. salt of an org. acid soluble in the org. solvent used for the fractionation (e.g., sodium acetate).
JP62199087A 1987-08-11 1987-08-11 Fractionation method of hyaluronic acid Expired - Lifetime JPH0813847B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62199087A JPH0813847B2 (en) 1987-08-11 1987-08-11 Fractionation method of hyaluronic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62199087A JPH0813847B2 (en) 1987-08-11 1987-08-11 Fractionation method of hyaluronic acid

Publications (2)

Publication Number Publication Date
JPS6443502A true JPS6443502A (en) 1989-02-15
JPH0813847B2 JPH0813847B2 (en) 1996-02-14

Family

ID=16401886

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62199087A Expired - Lifetime JPH0813847B2 (en) 1987-08-11 1987-08-11 Fractionation method of hyaluronic acid

Country Status (1)

Country Link
JP (1) JPH0813847B2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05508183A (en) * 1991-04-19 1993-11-18 フィディーア・ソシエタ・ペル・アチオニ Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology
US6946551B2 (en) 2003-03-12 2005-09-20 New Life Resources, Llc Preparation of hyaluronic acid from eggshell membrane
US8580315B2 (en) 2004-03-10 2013-11-12 Esm Technologies, Llc Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations
JP2014101470A (en) * 2012-11-22 2014-06-05 Sumitomo Bakelite Co Ltd Sugar chain purification method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS609042A (en) * 1983-06-09 1985-01-18 ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same
JPS60133894A (en) * 1983-11-25 1985-07-17 マイルス・ラボラトリース・インコーポレーテツド Hyaruronic acid and its production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS609042A (en) * 1983-06-09 1985-01-18 ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン Single-ended metal halogen lamp using ionized potential selection of added gas and method of producing same
JPS60133894A (en) * 1983-11-25 1985-07-17 マイルス・ラボラトリース・インコーポレーテツド Hyaruronic acid and its production

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05508183A (en) * 1991-04-19 1993-11-18 フィディーア・ソシエタ・ペル・アチオニ Hyaluronic acid purification method and fractionation of pure hyaluronic acid for ophthalmology
US6946551B2 (en) 2003-03-12 2005-09-20 New Life Resources, Llc Preparation of hyaluronic acid from eggshell membrane
US8580315B2 (en) 2004-03-10 2013-11-12 Esm Technologies, Llc Anti-inflammatory activity of eggshell membrane and processed eggshell membrane preparations
JP2014101470A (en) * 2012-11-22 2014-06-05 Sumitomo Bakelite Co Ltd Sugar chain purification method

Also Published As

Publication number Publication date
JPH0813847B2 (en) 1996-02-14

Similar Documents

Publication Publication Date Title
PL289487A1 (en) Method of producing new aminophenol derivatives
DE59804066D1 (en) Triazolinthion-phosphorsäure-derivate
BR9907679A (en) Sterile therapeutic peptide complex linked to a polysaccharide
HUT50767A (en) Process for producing new 1,3,4,5-tetrahydro-benz/c,d/indole derivatives and pharmaceutical compositions comprising such compounds
IL113450A (en) Process for the preparation of high solubility multicomponent inclusion complexes consisting of an acidic drug a cyclodextrin and a base and the complexes produced thereby
ES2169429T3 (en) Glycoconjugates of modified derivatives of CAMPTOTECHINE (LINK IN 20-O).
RU95105977A (en) INCLUSION COMPLEXES OF SALTS OF NIMESUlIDE WITH CYCLODEXTRINES, METHOD FOR THEIR PRODUCTION, PHARMACEUTICAL COMPOSITIONS ON THEIR BASE AND METHOD OF TREATMENT
CA1034128A (en) Amidocarbonylthiobarbituric acid derivatives, process for their preparation and their use as insecticides, and plant protection agents
KR950700310A (en) Heterotricyclic derivatives, methods for their preparation and pharmaceutical compositions containing them (HETEROTRICYCLIC DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM)
JPS6443502A (en) Fractionation of hyaluronic acid
CA2163197A1 (en) Tri(platinum) complexes
HU177823B (en) Rodenticide composition containing n-alkyl-2,4-dinitro-6-trifluoromethyl-diphenyl-amine derivatives and process for producing the active agents
EP0214017A3 (en) Use of n,n,-dimethylbiguanid p-chorophenoxyacetate in the treatment of neuropathies and nerval degeneration
NL7601378A (en) PROCESS FOR THE PREPARATION OF UNSATURATED POLYESTERAMIDS AND ARTICLES MANUFACTURED USING THE UNSATURIFIED POLYESTERAMIDES PREPARED IN THIS WAY.
DE3477924D1 (en) Benzenesulphonyl lactams, process for their preparation and their use as active ingredients in pharmaceutical compounds
TR199902775T2 (en) (S) -Camptothecin glycoconjugeleri.
JPS54105223A (en) Coating agent for solid medicine
EP0000741A3 (en) Indolacetoxy acetylamino acid derivatives, process for their preparation and their use as drugs
DE69012924D1 (en) Azole-substituted cycloalkanol derivatives, process for their preparation and use in agriculture and horticulture.
HU9201799D0 (en) New 2-amino-5-ciano-1,4-dihydropiridine-derivatives, process for their use and their use as medicaments
GR3024631T3 (en) Dyes, their preparation and use in the coloration of substrates.
ATE32897T1 (en) PYRAZOLO(3,4,5-KL)ACRIDINDERIVATE, PHARMACEUTICAL PREPARATIONS THEY CONTAIN AND PROCESS FOR THEIR MANUFACTURE.
ZA898775B (en) Cyclopropyl-substituted azolylmethylcarbinols,processes for their preparation and their use as medicaments
DE69618496D1 (en) Process for the derivatization of polyglucosamines
HRP960063B1 (en) Substituted benzenesulfonylureas and -thioureas, process for their preparation and their use in the production of pharmaceutical preparations as well as pharmaceutical preparations containing them