JPS6440000A - Method for fractionating and detecting hop stunt viroids - Google Patents

Method for fractionating and detecting hop stunt viroids

Info

Publication number
JPS6440000A
JPS6440000A JP19437787A JP19437787A JPS6440000A JP S6440000 A JPS6440000 A JP S6440000A JP 19437787 A JP19437787 A JP 19437787A JP 19437787 A JP19437787 A JP 19437787A JP S6440000 A JPS6440000 A JP S6440000A
Authority
JP
Japan
Prior art keywords
hsv
viroid
hop stunt
cucumber
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP19437787A
Other languages
Japanese (ja)
Other versions
JP2632317B2 (en
Inventor
Eishiro Yomo
Teruo Sano
Hiroshi Kudo
Nobutaka Sugimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UKI GOSEI KOGYO CO Ltd
Original Assignee
UKI GOSEI KOGYO CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UKI GOSEI KOGYO CO Ltd filed Critical UKI GOSEI KOGYO CO Ltd
Priority to JP62194377A priority Critical patent/JP2632317B2/en
Publication of JPS6440000A publication Critical patent/JPS6440000A/en
Application granted granted Critical
Publication of JP2632317B2 publication Critical patent/JP2632317B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To readily obtain hop stunt viroid such as hop stunt viroid cucumber variant useful in diagnosis of a plant pathogen, by detecting and fractionating the viroid with a specific DNA probe. CONSTITUTION:DNA is synthesized according to phosphotriester method to 15-25 mer synthetic DNA probe (A) containing a base sequence expressed by the formula (a is adenine, G is guanine, C is cytosine and T is thymine) and having complementarily with RNA of hop stunt viroid grape variant (HSV-g). Then a cucumber each infected to hop stunt viroid (HSV), hop stunt cucumber variant (HSV-c) and HSV-g is extracted to afford low-molecular RNA fraction (B) containing each viroid. Then components A and B are subjected to hybridimerization while changing temperature condition and strength and weakness of the reaction is labeled with radioisotope (e.g. <32>P) label to fractionate and provide the hop stunt viroids of HSV, HSV-g and HSV-g.
JP62194377A 1987-08-05 1987-08-05 Differential detection of hop dwarfed viroids Expired - Lifetime JP2632317B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62194377A JP2632317B2 (en) 1987-08-05 1987-08-05 Differential detection of hop dwarfed viroids

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62194377A JP2632317B2 (en) 1987-08-05 1987-08-05 Differential detection of hop dwarfed viroids

Publications (2)

Publication Number Publication Date
JPS6440000A true JPS6440000A (en) 1989-02-10
JP2632317B2 JP2632317B2 (en) 1997-07-23

Family

ID=16323581

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62194377A Expired - Lifetime JP2632317B2 (en) 1987-08-05 1987-08-05 Differential detection of hop dwarfed viroids

Country Status (1)

Country Link
JP (1) JP2632317B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619080B2 (en) * 2001-06-07 2009-11-17 Hiroshima University Oligonucleotides of a chicken leukemia inhibitory factor (LIF) gene
US8551439B2 (en) 2008-09-16 2013-10-08 Mitsubishi Materials Corporation Method of refining carbon parts for production of polycrystalline silicon

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF VIROLOGICAL METHODS=1985 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7619080B2 (en) * 2001-06-07 2009-11-17 Hiroshima University Oligonucleotides of a chicken leukemia inhibitory factor (LIF) gene
US8551439B2 (en) 2008-09-16 2013-10-08 Mitsubishi Materials Corporation Method of refining carbon parts for production of polycrystalline silicon

Also Published As

Publication number Publication date
JP2632317B2 (en) 1997-07-23

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