JPS6439989A - Production of useful gene product by filtration culture - Google Patents
Production of useful gene product by filtration cultureInfo
- Publication number
- JPS6439989A JPS6439989A JP19580787A JP19580787A JPS6439989A JP S6439989 A JPS6439989 A JP S6439989A JP 19580787 A JP19580787 A JP 19580787A JP 19580787 A JP19580787 A JP 19580787A JP S6439989 A JPS6439989 A JP S6439989A
- Authority
- JP
- Japan
- Prior art keywords
- phosphoric acid
- culture
- medium
- gene product
- give
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE:To readily and inexpensively obtain a gene product useful for mass production, by cultivating a specific transformed host cell in a medium containing phosphoric acid by a culture device of filtration type, filtering and removing phosphoric acid and recultivating. CONSTITUTION:A structural gene which manifests control of a promoter of an alkali phosphatase gene and codes the same (different) useful protein is bonded to the downstream of the promoter to give plasmid pYK269. Then Escherichia coli is transformed with the plasmid to give E.Coli MC1061/pYK269. Then the transformant is cultivated at a first stage of given time by a culture device of filtration type consisting of a culture tank 1 storing a medium 2 containing phosphoric acid, a filter 11 made of ceramic for cross flow filtration and a level controller 24 and phosphoric acid in the medium 2 is filtered and removed by the filter 2. Then a phosphoric acid-free medium is fed from a feed tank 26 to the tank 1 and culture is carried out at second stage to give a useful gene product such as beta-galactosidase.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62195807A JP2521102B2 (en) | 1987-08-05 | 1987-08-05 | ▲ Ro ▼ Production method of useful gene product by overculture |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62195807A JP2521102B2 (en) | 1987-08-05 | 1987-08-05 | ▲ Ro ▼ Production method of useful gene product by overculture |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6439989A true JPS6439989A (en) | 1989-02-10 |
JP2521102B2 JP2521102B2 (en) | 1996-07-31 |
Family
ID=16347308
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62195807A Expired - Fee Related JP2521102B2 (en) | 1987-08-05 | 1987-08-05 | ▲ Ro ▼ Production method of useful gene product by overculture |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2521102B2 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61152278A (en) * | 1984-12-26 | 1986-07-10 | Wakunaga Seiyaku Kk | Cultivation of microorganism |
-
1987
- 1987-08-05 JP JP62195807A patent/JP2521102B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61152278A (en) * | 1984-12-26 | 1986-07-10 | Wakunaga Seiyaku Kk | Cultivation of microorganism |
Also Published As
Publication number | Publication date |
---|---|
JP2521102B2 (en) | 1996-07-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
LAPS | Cancellation because of no payment of annual fees |