JPS6439989A - Production of useful gene product by filtration culture - Google Patents

Production of useful gene product by filtration culture

Info

Publication number
JPS6439989A
JPS6439989A JP19580787A JP19580787A JPS6439989A JP S6439989 A JPS6439989 A JP S6439989A JP 19580787 A JP19580787 A JP 19580787A JP 19580787 A JP19580787 A JP 19580787A JP S6439989 A JPS6439989 A JP S6439989A
Authority
JP
Japan
Prior art keywords
phosphoric acid
culture
medium
gene product
give
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP19580787A
Other languages
Japanese (ja)
Other versions
JP2521102B2 (en
Inventor
Takeshi Kobayashi
Shinji Iijima
Masakari Yamazaki
Koji Yoda
Toshiro Minami
Yoshihisa Kato
Takashi Ogawa
Shunji Yasuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coorstek KK
Original Assignee
Toshiba Ceramics Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Ceramics Co Ltd filed Critical Toshiba Ceramics Co Ltd
Priority to JP62195807A priority Critical patent/JP2521102B2/en
Publication of JPS6439989A publication Critical patent/JPS6439989A/en
Application granted granted Critical
Publication of JP2521102B2 publication Critical patent/JP2521102B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

PURPOSE:To readily and inexpensively obtain a gene product useful for mass production, by cultivating a specific transformed host cell in a medium containing phosphoric acid by a culture device of filtration type, filtering and removing phosphoric acid and recultivating. CONSTITUTION:A structural gene which manifests control of a promoter of an alkali phosphatase gene and codes the same (different) useful protein is bonded to the downstream of the promoter to give plasmid pYK269. Then Escherichia coli is transformed with the plasmid to give E.Coli MC1061/pYK269. Then the transformant is cultivated at a first stage of given time by a culture device of filtration type consisting of a culture tank 1 storing a medium 2 containing phosphoric acid, a filter 11 made of ceramic for cross flow filtration and a level controller 24 and phosphoric acid in the medium 2 is filtered and removed by the filter 2. Then a phosphoric acid-free medium is fed from a feed tank 26 to the tank 1 and culture is carried out at second stage to give a useful gene product such as beta-galactosidase.
JP62195807A 1987-08-05 1987-08-05 ▲ Ro ▼ Production method of useful gene product by overculture Expired - Fee Related JP2521102B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62195807A JP2521102B2 (en) 1987-08-05 1987-08-05 ▲ Ro ▼ Production method of useful gene product by overculture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62195807A JP2521102B2 (en) 1987-08-05 1987-08-05 ▲ Ro ▼ Production method of useful gene product by overculture

Publications (2)

Publication Number Publication Date
JPS6439989A true JPS6439989A (en) 1989-02-10
JP2521102B2 JP2521102B2 (en) 1996-07-31

Family

ID=16347308

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62195807A Expired - Fee Related JP2521102B2 (en) 1987-08-05 1987-08-05 ▲ Ro ▼ Production method of useful gene product by overculture

Country Status (1)

Country Link
JP (1) JP2521102B2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61152278A (en) * 1984-12-26 1986-07-10 Wakunaga Seiyaku Kk Cultivation of microorganism

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61152278A (en) * 1984-12-26 1986-07-10 Wakunaga Seiyaku Kk Cultivation of microorganism

Also Published As

Publication number Publication date
JP2521102B2 (en) 1996-07-31

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