JPS6430583A - Production of purified alpha-amylase-containing solution - Google Patents

Production of purified alpha-amylase-containing solution

Info

Publication number
JPS6430583A
JPS6430583A JP62185460A JP18546087A JPS6430583A JP S6430583 A JPS6430583 A JP S6430583A JP 62185460 A JP62185460 A JP 62185460A JP 18546087 A JP18546087 A JP 18546087A JP S6430583 A JPS6430583 A JP S6430583A
Authority
JP
Japan
Prior art keywords
amylase
alpha
solution
starch particles
starch
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62185460A
Other languages
Japanese (ja)
Other versions
JPH0640823B2 (en
Inventor
Masahiko Ishida
Ryoichi Haga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP62185460A priority Critical patent/JPH0640823B2/en
Publication of JPS6430583A publication Critical patent/JPS6430583A/en
Publication of JPH0640823B2 publication Critical patent/JPH0640823B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To produce a stable aqueous solution of enzyme, by efficiently separating and concentrating alpha-amylase from a low-purity solution containing alpha-amylase or a diluted solution containing alpha-amylase. CONSTITUTION:A solution obtained by removing a solid substance from a liquid culture solution of an alpha-amylase producing microorganisms or a crude alpha- amylase-containing solution such as an extracted solution obtained by extracting a solid culture mixture or a culture cell and removing a solid substance is brought into contact with starch particles at a temperature not to exceed a freezing point or not to cause substantial liquefying reaction of starch, the alpha-amylase is adsorbed on the starch particles and the starch particles having adsorbed the alpha-amylase are separated. The starch particles are washed with water at a temperature not to cause substantial liquefying reaction of starch, an alpha-amylase-containing solution obtained from the separated starch particles is treated with an enzyme containing at least one of glucoamylase and beta-amylase and hydrolyzed to a saccharide having <= molecular weight of oligosaccharide to produce purified alpha-amylase.
JP62185460A 1987-07-27 1987-07-27 Method for producing purified α-amylase-containing liquid Expired - Lifetime JPH0640823B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62185460A JPH0640823B2 (en) 1987-07-27 1987-07-27 Method for producing purified α-amylase-containing liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62185460A JPH0640823B2 (en) 1987-07-27 1987-07-27 Method for producing purified α-amylase-containing liquid

Publications (2)

Publication Number Publication Date
JPS6430583A true JPS6430583A (en) 1989-02-01
JPH0640823B2 JPH0640823B2 (en) 1994-06-01

Family

ID=16171175

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62185460A Expired - Lifetime JPH0640823B2 (en) 1987-07-27 1987-07-27 Method for producing purified α-amylase-containing liquid

Country Status (1)

Country Link
JP (1) JPH0640823B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445950A (en) * 1991-10-31 1995-08-29 Director Of National Food Research Institute Ministry Of Agriculture, Forestry And Fisheries Method of using α-amylase to prepare slightly decomposed starch granules having low viscosity

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445950A (en) * 1991-10-31 1995-08-29 Director Of National Food Research Institute Ministry Of Agriculture, Forestry And Fisheries Method of using α-amylase to prepare slightly decomposed starch granules having low viscosity

Also Published As

Publication number Publication date
JPH0640823B2 (en) 1994-06-01

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