JPS6429400A - Monoclonal antibody - Google Patents

Monoclonal antibody

Info

Publication number
JPS6429400A
JPS6429400A JP62184921A JP18492187A JPS6429400A JP S6429400 A JPS6429400 A JP S6429400A JP 62184921 A JP62184921 A JP 62184921A JP 18492187 A JP18492187 A JP 18492187A JP S6429400 A JPS6429400 A JP S6429400A
Authority
JP
Japan
Prior art keywords
cells
cea
fused
mouse
nca
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62184921A
Other languages
Japanese (ja)
Inventor
Akio Hirohashi
Yukio Shimozato
Yoichi Sakurai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Kayaku Co Ltd
Original Assignee
Nippon Kayaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Kayaku Co Ltd filed Critical Nippon Kayaku Co Ltd
Priority to JP62184921A priority Critical patent/JPS6429400A/en
Publication of JPS6429400A publication Critical patent/JPS6429400A/en
Pending legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

NEW MATERIAL:A monoclonal antibody reactive with carcinoembryonic antigen (CEA), nonspecific cross-reactive antigen (NCA) and biliary glycoprotein I (BGP-1), especially with the CEA. USE:Serological assay of CEA, cytological and histopathological diagnosis of cancer and mutual discrimination of the CEA, NCA and BGP-1, etc. PREPARATION:A mouse is initially immunized with cancerous cells, etc. The amount inoculated is preferably 10<5>-10<8> cells per mouse at a time. The spleen is then extirpated from the mouse and subjected to centrifugation to provide cells capable of producing antibodies. The resultant cells are subsequently fused to cells having the self-propagation ability to afford fused cell, which are then separated by a limiting dilution method. After propagating the separated fused cells, antibodies produced in respective holes (wells) are reacted with various cells, etc., by a fluorescent antibody technique, etc. Sorted fused cell are subsequently cultivated in an incubator to provide the aimed antibody from the supernatant liquid.
JP62184921A 1987-07-24 1987-07-24 Monoclonal antibody Pending JPS6429400A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62184921A JPS6429400A (en) 1987-07-24 1987-07-24 Monoclonal antibody

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62184921A JPS6429400A (en) 1987-07-24 1987-07-24 Monoclonal antibody

Publications (1)

Publication Number Publication Date
JPS6429400A true JPS6429400A (en) 1989-01-31

Family

ID=16161660

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62184921A Pending JPS6429400A (en) 1987-07-24 1987-07-24 Monoclonal antibody

Country Status (1)

Country Link
JP (1) JPS6429400A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010098471A1 (en) * 2009-02-27 2010-09-02 株式会社バイオマトリックス研究所 Immunization method using cancer cell
WO2012026615A1 (en) * 2010-08-25 2012-03-01 株式会社バイオマトリックス研究所 Method for producing antibodies using cancer cells
US8789021B2 (en) 2005-06-30 2014-07-22 International Business Machines Corporation Method and apparatus for object-oriented load testing of computing systems

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500427A (en) * 1983-01-21 1985-03-28 プリマス，フレデリツク　ジエ−ムス Specificity CEA Family - Antigens, Antibodies Specific Thereto and Methods of Their Use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500427A (en) * 1983-01-21 1985-03-28 プリマス，フレデリツク　ジエ−ムス Specificity CEA Family - Antigens, Antibodies Specific Thereto and Methods of Their Use

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8789021B2 (en) 2005-06-30 2014-07-22 International Business Machines Corporation Method and apparatus for object-oriented load testing of computing systems
WO2010098471A1 (en) * 2009-02-27 2010-09-02 株式会社バイオマトリックス研究所 Immunization method using cancer cell
JP5688699B2 (en) * 2009-02-27 2015-03-25 株式会社オーダーメードメディカルリサーチ Immunization using cancer cells
WO2012026615A1 (en) * 2010-08-25 2012-03-01 株式会社バイオマトリックス研究所 Method for producing antibodies using cancer cells
US8598408B2 (en) 2010-08-25 2013-12-03 Ordermade Medical Research Inc. Method of producing an antibody using a cancer cell

Similar Documents

Publication Publication Date Title
JPS6447378A (en) Monoclonal antibody to black tumor related antigen, hybrid cell line for producing the same and use thereof
EP0677533A3 (en) Antibodies against epitopes with homology to self antigens, methods of preparation and applications thereof
IL102294A0 (en) Ctla4 receptor protein and its use
EP0404912A4 (en) Monoclonal antibodies reactive with defined regions of the t cell antigen receptor
AU6054398A (en) Stimulation of an immune response with antibodies labeled with the alpha-galactosyl epitope
IE883062L (en) Monoclonal antibodies
EP0431541A3 (en) Toxoplasma gondii antigen, its production and use
EP0118893A3 (en) Human monoclonal antibodies from lymphocytes of patients with malignant melanoma
ATE556091T2 (en) MONOCLONAL ANTIBODIES PRODUCTION THROUGH EBV TRANSFORMATION OF B CELLS
JPS6429400A (en) Monoclonal antibody
EP0323802A3 (en) Monoclonal antibodies against, and cell surface antigen of, lung carcinoma
EP0104014A3 (en) Cell surface antigens of human astrocytoma
AU2911289A (en) Carcinoma-associated antigens, and antibodies which recognize these antigens
ATE283927T1 (en) MONOCLONAL ANTIBODIES AND ANTIGENS RELATED TO HUMAN LUNG ADENOCARCINOMA AND IMMUNOASSAY METHODS USING THE SAME
GB2199833A (en) Preparation of monoclonal antibodies
ES8501530A1 (en) Adult t-cell leukemia antigens, methods for their preparation and for assaying antibodies thereto
ATE187463T1 (en) MONOCLONAL ANTIBODIES AGAINST TUMOR-ASSOCIATED ANTIGENS, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE
EP0158291A3 (en) A method for purifying carcinoembryonic antigen and a method for producing a carcinoembryonic antigen-reactive monoclonal antibody
PH21459A (en) Process for assaying atl virus antibody and reagent therefor
DE69020914D1 (en) Monoclonal antibodies from rats to human antigens and methods for their production.
DE69813555D1 (en) AGAINST THE SURFACE OF CRYPTOSPORIDIUM OOCYST-TESTED IGG1 CLASS ANTIBODIES
JPS643199A (en) Monoclonal antibody
Bussard Plaque morphology studied with immune hybridomas
JPS6471480A (en) Cell line producing monoclonal antibody and monoclonal antibody produced therefrom
JPS6459070A (en) Immunoassay method