JPS6423896A - Method for enzymic reaction using microbial cell - Google Patents
Method for enzymic reaction using microbial cellInfo
- Publication number
- JPS6423896A JPS6423896A JP62180623A JP18062387A JPS6423896A JP S6423896 A JPS6423896 A JP S6423896A JP 62180623 A JP62180623 A JP 62180623A JP 18062387 A JP18062387 A JP 18062387A JP S6423896 A JPS6423896 A JP S6423896A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- water
- washed
- microbial cells
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000813 microbial effect Effects 0.000 title abstract 6
- 244000005700 microbiome Species 0.000 abstract 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 6
- 102000004882 Lipase Human genes 0.000 abstract 4
- 108090001060 Lipase Proteins 0.000 abstract 4
- 239000004367 Lipase Substances 0.000 abstract 4
- 150000002148 esters Chemical class 0.000 abstract 4
- 235000019421 lipase Nutrition 0.000 abstract 4
- 239000002798 polar solvent Substances 0.000 abstract 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 abstract 3
- 239000003921 oil Substances 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 239000012454 non-polar solvent Substances 0.000 abstract 2
- 150000001298 alcohols Chemical class 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 239000000306 component Substances 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 239000003925 fat Substances 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To utilize a microorganism having the ability to produce lipase for lipase enzymic reaction, by cultivating the microorganism, washing the aforementioned microorganism with water or directly using the microorganism or replacing water with a polar solvent and then nonpolar solvent in using for ester interchange or synthetic reaction in an oil system. CONSTITUTION:A microorganism having the ability to produce lipase is cultivated in a culture medium, filtered off and washed with water several times to remove culture medium components, etc., contained in the microorganism. When the microorganism is utilized for hydrolytic reaction of fats and oils or ester synthetic reaction of glycerol or other alcohols with fatty acids, the microbial cells washed with water are directly used as a catalyst. When the microorganism is used for ester interchange or ester synthetic reaction in an oil system, the microbial cells washed with water are washed with a polar solvent, such as acetone, to replace water contained in the microbial cells with the polar solvent and dehydrate the microbial cells. Furthermore, the dehydrated microbial cells are washed with a nonpolar solvent, such as hexane, to replace the polar solvent therewith and enzymic reaction is carried out utilizing lipase contained in the above-mentioned microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62180623A JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62180623A JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6423896A true JPS6423896A (en) | 1989-01-26 |
Family
ID=16086448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62180623A Pending JPS6423896A (en) | 1987-07-20 | 1987-07-20 | Method for enzymic reaction using microbial cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6423896A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7087418B2 (en) | 2001-12-19 | 2006-08-08 | Bristol-Myers Squibb Company | Pichia pastoris formate dehydrogenase and uses therefor |
-
1987
- 1987-07-20 JP JP62180623A patent/JPS6423896A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7087418B2 (en) | 2001-12-19 | 2006-08-08 | Bristol-Myers Squibb Company | Pichia pastoris formate dehydrogenase and uses therefor |
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