JPS6397668A - Sunproof carotene - Google Patents

Abstract

PURPOSE:To provide the title carotene which retains stably its color tone even when stored in the presence of oxygen, water, air, etc., under the irradiation with sunlight over a long period of time and is suitable for use as a food additive, by microencapsulating (oil-diluted) carotene with a specified shell-forming material. CONSTITUTION:Carotene (a) (e.g., one which is extracted from palm oil, concentrated and refined) is optionally diluted with food oil (b) (e.g., colza oil) to obtain (oil-diluted) carotene (A). Separately, 1-300wt% sunproofing agent (c) having a particle size of 0.01-50mum (e.g., TiO2) or 0.5-20wt% ultraviolet absorber (d) (e.g., alpha-bisabolol) is dispersed in a shell-forming material (e) (e.g., gelatin) to obtain a shell-forming material (B) contg. the component (c) or (d). The component A is microencapsulated by using the component B to obtain the title carotene having a particle size of 10-3,000mum, pref. 50-2,000mum.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a light-resistant carotene that exhibits excellent stability with almost no decolorization or fading even when added to general foods and stored for a long period of time.

In recent years, carotenoids have been widely used as yellow or red natural coloring agents in the food, pharmaceutical, and cosmetic fields. In particular, among various carotenoids, carotene is metabolized in the human body and converted into vitamin A, so it is an indispensable substance for health and is highly useful.

However, carotene has the disadvantage that it bleaches or fades when it comes into contact with oxygen, water, air, etc. or is exposed to sunlight, and its color tone is unstable. Therefore, in order to improve this drawback, carotene is emulsified using an emulsion stabilizer and stabilized by drying (Japanese Patent Publication No. 35-8095, Japanese Patent Application Laid-open No. 49-126727), or sugars are added before or after emulsification. In addition, it stabilizes carotene (Japanese Patent Application Laid-open No. 54-13
Methods such as No. 5822 and Japanese Unexamined Patent Publication No. 137410/1982 have been proposed.

These methods improve the stability of carotene, but
Even if these methods are used to stabilize carotene, it will discolor when stored for a long period of time, and these methods cannot prevent the effects of sunlight, which has the greatest effect on carotene discoloration.

1" There was a problem in that carotene faded in just 4 to 5 days when irradiated with light.

For this reason, rA9!, a carotene stabilization method that can maintain a stable color tone for a long time even when incorporated into various products such as food! was requested.

The present invention was made in view of the above circumstances, and an object of the present invention is to provide a light-resistant carotene that can maintain a stable color tone for a long period of time even in the presence of MJ, water, air, etc., and even under sunlight irradiation. shall be.

The inventors of the present invention
As a result of intensive studies to solve the above problems, we found that carotene or oil-diluted carotene was microencapsulated in a film material containing dispersed light shielding agents and ultraviolet absorbers, and by encapsulating carotene in the film material, oxygen Since it is completely shielded from water, air, etc., the color tone of carotene is almost never bleached or faded due to decomposition due to contact with oxygen, water, air, etc. during storage, and the color tone of carotene is maintained stably. In particular, due to the light-shielding effect of the light-shielding agent and ultraviolet absorber in the membrane material, carotene is shielded from sunlight, which has the greatest effect on carotene fading, so decomposition of carotene due to sunlight irradiation can be reliably prevented. The present inventors have discovered that such a carotene encapsulating agent can maintain the color tone of carotene extremely stably for a long period of time even when incorporated into various products such as foods, and have thus come up with the present invention.

Therefore, an object of the present invention is to provide light-resistant carotene, which is characterized by microcapsulating carotene or oil-diluted carotene in a membrane material containing a light-shielding agent or an ultraviolet absorber dispersed therein.

The present invention will be described in further detail below.

The carotene used in the present invention is preferably a natural carotene, and particularly preferably a carotene extracted, concentrated, and purified from palm oil. The carotene obtained from this palm oil is α, β
It is a red, opaque oil containing 60 to 70% of all-trans β-carotene.

As the carotene, various carotenes may be used alone or in combination of two or more, and carotenoids such as cryptoxanthin and canthaxanthin may be used.

Furthermore, in the present invention, it is preferable to use the above-mentioned carotene after diluting it with an oil or fat, particularly an edible oil or fat. In this case, carotene may be dissolved in edible oil or fat to form an edible oil or fat solution, or carotene may be dispersed in edible oil or fat.
In this way, by mixing carotene with edible fats and oils, it becomes possible to obtain a uniform solution and to ensure any color depending on the dilution rate.

Here, animal and vegetable oils can be suitably used as the edible fats, and specifically, soybean oil, peanut oil, sunflower oil, rapeseed oil, corn oil, cottonseed oil, coconut oil, palm oil, etc. are used as the vegetable oil, and animal oils include soybean oil, peanut oil, sunflower oil, rapeseed oil, etc. Examples include beef tallow, lard, fish oil, and squalene. These may be used alone or in combination of two or more.

Further, the blending ratio of carotene and fat and oil is not particularly limited, but it is preferable that the blending ratio of carotene to fat and oil is 0.01 to 30% by weight.

In the present invention, carotene or carotene diluted with the oil or fat described above is coated with a membrane material to form an encapsulating agent. In this case, various membrane materials can be selected, but a carotene coating is possible, and a substance that dissolves in water or hot water and is not harmful to the physiological effects of the human body can be suitably used. Specific examples of such membrane materials include gelatin, protein, gum arabic,
Examples include carboxymethylcellulose and alginates, with gelatin, gum arabic, carboxymethylcellulose and alginates being particularly preferred.

Furthermore, in the present invention, the above-mentioned film material containing a light shielding agent or an ultraviolet absorber dispersed therein is used.
In this way, by coating carotene with a film material containing dispersed light-shielding agents and ultraviolet absorbers, carotene can be made light-resistant, and carotene can be completely blocked from oxygen, water, air, etc., so the present invention The decomposition of carotene by sunlight, oxygen, water, air, etc. when the product is blended into food and stored is effectively prevented, and the color tone of carotene can be stably maintained.

In this case, the light shielding agent and ultraviolet absorber are selected appropriately, but
As light shielding agents, fine particles of water-insoluble inorganic compounds such as titanium dioxide, calcium carbonate, calcium phosphate, kaolin, talc, and maifan stone, fine particles of water-insoluble organic compounds such as starch (cornstarch, potato starch, etc.), casein, and fish scale bacterium; Examples include aluminum lake of iron sesquioxide 1 food coloring, water-insoluble colorants such as copper chlorophyll, metal foils or fine powders of gold, silver, etc. Among these light-shielding agents, fine particles of titanium dioxide, iron sesquioxide, gold, and iron can be suitably used, and in particular, the particle size is preferably 0.01 to 50μ.
s, more preferably 0.01 to 207 J. In addition, the amount added is 1 to 300% by weight based on the membrane material.
In particular, it is preferably 10 to 250% by weight. If the amount added is less than 1%, the light shielding properties will be poor, and if it is more than 300%, uniform dispersion will be difficult. In addition, when using titanium dioxide as a light shielding agent, titanium dioxide is used as a film material at a concentration of 150 to 2
When added in an amount of 0.00% by weight, almost 100% of sunlight can be blocked. Moreover, when using titanium dioxide, it is desirable to disperse titanium dioxide in 0.5 to 3 times the amount of water and add it.

This prevents agglomeration of titanium dioxide and allows it to be well dispersed and contained in the membrane material.

In addition, as ultraviolet absorbers, γ-oryzanol, α-
Bisabrol (contains 50% in mustard oil).

benzoquinones such as lauson, 'nigerone, anthraquinones such as antholone, anthranol, aloin, heliclidine-5-flavonoid, abigenin, quercetin, quercitrin, hyperin.

Natural ultraviolet absorbers such as flavonoids such as rutin, bergaptenurocanic acid, umbelliferone, and extracts containing these are preferred, and if they are insoluble in pond water, they can be selectively incorporated into the capsule membrane for use. be able to. Here, the amount added is not particularly limited, but is preferably 0.5 to 20% by weight based on the membrane material.

Capsule manufacturing methods are appropriately selected depending on the purpose of use and membrane material, etc., and include spray drying, air suspension coating, powder bed method, liquid curing method, phase separation method, melt dispersion cooling method, and interfacial polymerization method. , 1ns
Any method for producing microcapsules, such as the in-tu polymerization method or the in-liquid curing method, may be used, but phase separation methods (complex coacervation method, salt coacervation method), in-liquid curing method, etc. Suitably adopted.

Moreover, the particle size of the capsule is preferably 10 to 3,000 ts, particularly preferably 50 to 2,000 tun. Particle size is 3o0
If it is larger than 0p, the taste becomes poor when the encapsulating agent is blended into foods, etc., and if it is smaller than 10p, the film thickness becomes thinner depending on the particle size, resulting in poor storage stability.

The encapsulating agent of the present invention can be used in various products, especially health drinks and separate dressings.

It can be effectively used in general foods such as gum and jelly.

As explained above, the carotene encapsulating agent of the present invention is obtained by coating carotene with a film material containing a light-shielding agent or a film material that disperses and contains ultraviolet rays and encapsulating the carotene. Even in the presence of water or sunlight, the carotene decomposes and the color tone rarely fades or fades. Therefore, foods using the product of the present invention can have a longer shelf life than conventional products, and can be effectively used as nutritionally fortified foods containing vitamin A precursors.

EXAMPLES Hereinafter, the present invention will be specifically explained with reference to examples, but the present invention is not limited to the following examples.

[Example 1] 25 g of acid-treated gelatin (jelly strength 300 bloom) and 25 g of gum arabic were added to 950 g of water and dissolved by heating to 40°C.

Next, a mixture of 40 g of titanium dioxide and 40 g of water was added to this aqueous solution, and after stirring and dispersing it uniformly, a mixture of 140 g of rapeseed oil and 60 g of carotene was dispersed under stirring to reduce the particle size. Adjusted to 200-5004.

Furthermore, it was heated to 40°C, and a 20% acetic acid aqueous solution was added to p
After adjusting H to 4.2, the mixture was cooled to 10° C. to obtain microcapsules having a substantially spherical shape and a particle size of 200 to 500 Am. The capsules were separated using a sieve, washed with water at 10°C, and then dried with dry air at 20°C (40% humidity).

The capsules obtained by the above method were left outdoors in a place with good sunlight, and the amount of carotene remaining in the capsules was determined orally. The amount of carotene was determined by the following method. As a control, a commercially available carotene dye was adjusted to the same concentration as the capsule and left outdoors in the same way as the capsule.

The Yudanten λ Lidingying capsule solution was heated to 60°C, and the capsules were broken and used as samples. The sample and control solution were each diluted with water, a certain amount of which was extracted with cyclohexane, and then extracted with a wavelength of 455 nm.
The absorbance [D] was measured, and the carotene content and carotene residual rate were calculated using the following formula.

The results are shown in Table 1.

Residual rate (%) = city x 100 Co = amount of carotene before sunlight irradiation C = amount of carotene after a certain period of time Table 1 [Example 2] 25 g of acid-treated gelatin (jelly strength 300 bloom) and Carbodine were added to 965 g of water. Sodium oxymethylcellulose (degree of polymerization 100-150) 1 Og was dissolved at 40°C,
This was divided into two parts of 500 g each. Add 3.5 g of chamomile essential oil to one of the two divided solutions using a homogenizer to obtain a particle size of 1.
A chamomile essential oil dispersion was prepared by dispersing the oil to a length of ~27 m.

In addition, 140 g of safflower oil containing 30% carotene was dispersed in the other divided solution so that the particle size was 300 to 1000 ps. Subsequently, 500 g of the chamomile essential oil dispersion was added thereto, and a 20% acetic acid aqueous solution was further added at 40°C to adjust the pH to 3.9. Thereafter, it is cooled to 10°C, and the particle size is 3, which contains fine droplets of chamomile essential oil in the capsule wall and encapsulates a safsa flower containing 30% carotene.
Almost spherical microcapsules of 00 to 1000 u were obtained. The capsules were washed with a 15% aqueous sodium sulfate solution at 20°C, separated using a sieve, and dried with dry air at 20°C.

The stability of the carotene content in the microcapsules thus obtained was measured in the same manner as in Example 1. The results are shown in Table 2.

Table 2 [Example 3] Acid-treated gelatin (jelly strength 250 bloom) aog was added to 920 g of water and dissolved at 40°C.

40 g of iron sesquioxide was added and uniformly dispersed with stirring. Next, 300 g of soybean oil containing 35% carotene was dispersed in this aqueous solution with stirring, followed by 20% sulfuric acid solution.
After adding 670 g of helium aqueous solution, capsules having a particle size of 200 to 5 oO/jJI+ were obtained by cooling at 20°C. The capsules were separated by filtration, washed with a 15% aqueous sodium sulfate solution, and then dried in dry air at 20°C.

The carotene content and residual rate of the capsules were measured and calculated in the same manner as in Example 1.

The results are shown in Table 3.

Table 3 From the results shown in Tables 1 to 3, it was confirmed that compared to conventional commercially available carotene pigments, the microcapsules containing natural carotene of the present invention can store carotene stably for an extremely long period of time.

[Example 4] After cooling the capsules prepared in the same manner as in Example 1 to 5° C., 500 d of 0.05% tannic acid aqueous solution was gradually added, and the capsules were stirred for 30 minutes for hardening treatment. The capsules were separated, washed and dried to obtain carotene-containing capsules.

[Example 5] Capsules were prepared in the same manner as in Example 3 except that the particle size was adjusted to 100 to 200p, and after cooling to 5°C,
% alum potassium aqueous solution (pH 4) was gradually added and stirred for 2 hours for hardening treatment. The capsules were separated, washed and dried to obtain carotene-containing capsules.

The hardened capsules described above also had excellent carotene stability.

Claims (1)

[Claims] 1. A light-resistant carotene characterized by microcapsulating carotene or oil-diluted carotene in a membrane material containing a light-shielding agent or an ultraviolet absorber dispersed therein.