JPS6371172A - 生体細胞の凍結方法 - Google Patents
生体細胞の凍結方法Info
- Publication number
- JPS6371172A JPS6371172A JP61217685A JP21768586A JPS6371172A JP S6371172 A JPS6371172 A JP S6371172A JP 61217685 A JP61217685 A JP 61217685A JP 21768586 A JP21768586 A JP 21768586A JP S6371172 A JPS6371172 A JP S6371172A
- Authority
- JP
- Japan
- Prior art keywords
- chamber
- cells
- freezing
- cell
- living cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000007710 freezing Methods 0.000 title claims abstract description 25
- 230000008014 freezing Effects 0.000 title claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000001307 helium Substances 0.000 claims abstract description 13
- 229910052734 helium Inorganic materials 0.000 claims abstract description 13
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 abstract description 20
- 230000004083 survival effect Effects 0.000 abstract description 7
- 239000002245 particle Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 50
- 238000007796 conventional method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000010583 slow cooling Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000007798 antifreeze agent Substances 0.000 description 3
- 239000002577 cryoprotective agent Substances 0.000 description 3
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002900 effect on cell Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61217685A JPS6371172A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61217685A JPS6371172A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6371172A true JPS6371172A (ja) | 1988-03-31 |
| JPH0534953B2 JPH0534953B2 (enrdf_load_stackoverflow) | 1993-05-25 |
Family
ID=16708115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61217685A Granted JPS6371172A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6371172A (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004017732A1 (de) * | 2002-08-13 | 2004-03-04 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Kryokonservierung mit einem gas- oder dampfförmigen kühlmedium |
| JP2014500963A (ja) * | 2010-11-24 | 2014-01-16 | フラウンホーファー−ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウ | 低温保存された生体試料を監視するための方法および装置 |
-
1986
- 1986-09-16 JP JP61217685A patent/JPS6371172A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004017732A1 (de) * | 2002-08-13 | 2004-03-04 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Kryokonservierung mit einem gas- oder dampfförmigen kühlmedium |
| JP2014500963A (ja) * | 2010-11-24 | 2014-01-16 | フラウンホーファー−ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウ | 低温保存された生体試料を監視するための方法および装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0534953B2 (enrdf_load_stackoverflow) | 1993-05-25 |
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