JPH0512691Y2 - - Google Patents
Info
- Publication number
- JPH0512691Y2 JPH0512691Y2 JP11617587U JP11617587U JPH0512691Y2 JP H0512691 Y2 JPH0512691 Y2 JP H0512691Y2 JP 11617587 U JP11617587 U JP 11617587U JP 11617587 U JP11617587 U JP 11617587U JP H0512691 Y2 JPH0512691 Y2 JP H0512691Y2
- Authority
- JP
- Japan
- Prior art keywords
- helium
- cylinder
- nitrogen
- shield
- freezing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 92
- 239000001307 helium Substances 0.000 claims description 66
- 229910052734 helium Inorganic materials 0.000 claims description 66
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 66
- 229910052757 nitrogen Inorganic materials 0.000 claims description 46
- 238000007710 freezing Methods 0.000 claims description 36
- 230000008014 freezing Effects 0.000 claims description 36
- 239000007789 gas Substances 0.000 claims description 29
- 239000007788 liquid Substances 0.000 claims description 27
- 238000005138 cryopreservation Methods 0.000 claims description 12
- 238000011084 recovery Methods 0.000 claims description 10
- 239000007853 buffer solution Substances 0.000 claims description 8
- 238000005273 aeration Methods 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 63
- 238000001816 cooling Methods 0.000 description 22
- 238000009423 ventilation Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- 238000011109 contamination Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000010583 slow cooling Methods 0.000 description 4
- 239000002577 cryoprotective agent Substances 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229910000881 Cu alloy Inorganic materials 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000007798 antifreeze agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11617587U JPH0512691Y2 (enrdf_load_stackoverflow) | 1987-07-29 | 1987-07-29 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11617587U JPH0512691Y2 (enrdf_load_stackoverflow) | 1987-07-29 | 1987-07-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6422975U JPS6422975U (enrdf_load_stackoverflow) | 1989-02-07 |
| JPH0512691Y2 true JPH0512691Y2 (enrdf_load_stackoverflow) | 1993-04-02 |
Family
ID=31358470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11617587U Expired - Lifetime JPH0512691Y2 (enrdf_load_stackoverflow) | 1987-07-29 | 1987-07-29 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0512691Y2 (enrdf_load_stackoverflow) |
-
1987
- 1987-07-29 JP JP11617587U patent/JPH0512691Y2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6422975U (enrdf_load_stackoverflow) | 1989-02-07 |
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