JPS6364744B2 - - Google Patents
Info
- Publication number
- JPS6364744B2 JPS6364744B2 JP10982681A JP10982681A JPS6364744B2 JP S6364744 B2 JPS6364744 B2 JP S6364744B2 JP 10982681 A JP10982681 A JP 10982681A JP 10982681 A JP10982681 A JP 10982681A JP S6364744 B2 JPS6364744 B2 JP S6364744B2
- Authority
- JP
- Japan
- Prior art keywords
- test tube
- rotor
- holder
- magnetic element
- tube holder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000005406 washing Methods 0.000 claims description 12
- 210000000601 blood cell Anatomy 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims 1
- 239000006228 supernatant Substances 0.000 description 11
- 210000003743 erythrocyte Anatomy 0.000 description 7
- 239000002504 physiological saline solution Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- -1 centrifugation Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Centrifugal Separators (AREA)
Description
【発明の詳細な説明】
本発明は血球洗浄用遠心機に係り、特に自動的
に液の注入、遠心分離、上澄排出操作を行うのに
好適な構造の血球洗浄用遠心機に関するものであ
る。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood cell washing centrifuge, and more particularly to a blood cell washing centrifuge having a structure suitable for automatically performing liquid injection, centrifugation, and supernatant discharge operations. .
従来の間抵抗グロブリン試験（クームス試験）
は、赤血球の塩性懸濁液（生理食塩水）を遠心分
離し、その上澄をすてて、また生理食塩水を注入
して塩性懸濁液を作り、また遠心分理してその上
澄液をすてるという赤血球洗浄操作を数回繰り返
してから行われる。ところで、赤血球洗浄の場
合、従来は上澄液をすてるときの操作を人為的に
行うようにしていたので、試験結果の再現性を良
好にするためには、相当の経験と技量を要求され
るという問題があつた。 Conventional resistance globulin test (Coombs test)
centrifuges a saline suspension of red blood cells (physiological saline), discards the supernatant, injects physiological saline again to make a saline suspension, and centrifuges it again. This is done after repeating the red blood cell washing procedure several times by discarding the supernatant. By the way, in the case of washing red blood cells, the operation when discarding the supernatant liquid has traditionally been performed artificially, so a considerable amount of experience and skill is required to ensure good reproducibility of test results. There was a problem.
本発明は上記に鑑みてなされたもので、その目
的とするところは、多数の検体を短時間の間に同
一条件で洗浄処理できる血球洗浄用遠心機を提供
することにある。 The present invention has been made in view of the above, and an object of the present invention is to provide a blood cell washing centrifuge that can wash a large number of specimens under the same conditions in a short period of time.
本発明の特徴は、モータに連結されて回転する
ロータ上に円形に配設され、回動自在で上記ロー
タ回転時に遠心力で垂直方向から水平方向に回動
し、分配素子によつてある角度に保持される試験
管ホルダに装着された試験管内に同じく回転して
いる上記分配素子から血球洗浄用液体を注入して
懸濁液を作り、引き続き上記ロータを回転させて
上記懸濁液を遠心処理してから上記ロータを停止
させ、この時点で磁気素子を内周上部に取り付け
た保持体を上方に移動させ、上記試験管ホルダー
に設けた磁気素子に生ずる反発力によつて上記試
験管ホルダを垂直方向の位置に保持させ、この状
態で上記ロータを再び回転させて上記試験管内の
上澄液を遠心力で上記試験管外へ放出させ得る構
成とした点にある。 A feature of the present invention is that the rotor is arranged in a circular shape on a rotating rotor connected to a motor, is rotatable, rotates from the vertical direction to the horizontal direction due to centrifugal force when the rotor rotates, and is rotated at a certain angle by a distribution element. Blood cell washing liquid is injected from the distribution element which is also rotating into a test tube attached to a test tube holder held in the test tube to create a suspension, and the suspension is centrifuged by subsequently rotating the rotor. After processing, the rotor is stopped, and at this point, the holder with the magnetic element attached to the upper part of the inner circumference is moved upward, and the repulsive force generated in the magnetic element provided on the test tube holder causes the test tube holder to is held in a vertical position, and in this state, the rotor is rotated again to discharge the supernatant liquid in the test tube out of the test tube by centrifugal force.
以下本発明を図に示した実施例を用いて詳細に
説明する。 The present invention will be explained in detail below using examples shown in the drawings.
図は本発明の血球洗浄用遠心機の一実施例を示
す縦断面図である。図において、１はモータ、２
は駆動軸、３はロータで、ロータ３はモータ１に
駆動軸２を介して連結されており、モータ１によ
つて回転駆動される。４はロータ３上に円形に回
動自在に配設された下方に磁気素子５を設けてあ
る試験管ホルダーで、試験管ホルダー４は複数個
設けてあり、試験管ホルダー４には赤血球（血
液）を入れた試験管６が装着してあり、試験管ホ
ルダー４は、ロータ３をある回転数で回転させる
と、遠心力によつて垂直方向から水平方向に移動
する。７は分配素子で、分配素子７は、試験管ホ
ルダー４が遠心力で水平方向に移動したとき、あ
る角度以上に移動しないように保持するように構
成してある。分配素子７内には生理食塩水が入れ
てあり、分配素子７がロータ３とともに回転する
と、この生理食塩水が遠心力で分配素子７内を均
一に移動し、分配素子７によつて図示２点鎖線で
示すある角度に保持された試験管ホルダー４の試
験管６内に分配素子７に設けてあるノズル８から
勢いよく噴出され、試験管６内の赤血球と混合し
て洗浄作用を行う。この状態で引き続きロータ３
を回動させてこの塩性懸濁液を遠心処理すると、
赤血球は遠心分離されて沈でんし、上方に上澄液
が残る。 The figure is a longitudinal cross-sectional view showing one embodiment of a blood cell washing centrifuge according to the present invention. In the figure, 1 is a motor, 2
3 is a drive shaft, and 3 is a rotor. The rotor 3 is connected to a motor 1 via a drive shaft 2, and is rotationally driven by the motor 1. Reference numeral 4 denotes a test tube holder which is rotatably arranged in a circular manner on the rotor 3 and has a magnetic element 5 at the bottom. ) is attached to the test tube holder 4, and when the rotor 3 is rotated at a certain number of rotations, the test tube holder 4 moves from the vertical direction to the horizontal direction due to centrifugal force. 7 is a distribution element, and the distribution element 7 is configured to hold the test tube holder 4 so that it does not move beyond a certain angle when the test tube holder 4 is moved horizontally by centrifugal force. Physiological saline is contained in the distributing element 7, and when the distributing element 7 rotates together with the rotor 3, this physiological saline is uniformly moved within the distributing element 7 by centrifugal force, and the distributing element 7 moves the saline as shown in the figure. The liquid is vigorously ejected from a nozzle 8 provided in the distribution element 7 into the test tube 6 of the test tube holder 4 held at a certain angle as shown by the dotted chain line, and mixes with the red blood cells in the test tube 6 to perform a cleaning action. Continue to rotor 3 in this state.
When this salt suspension is centrifuged by rotating the
The red blood cells are centrifuged and sedimented, leaving a supernatant at the top.
９は内周上部に磁気素子１０を取り付けてある
保持体で、保持体９は電磁石１１を励磁すると上
方に移動し、電磁石１１の励磁を中止すると、ば
ね１２の作用によりもとの位置に引き下げられる
ように構成してあり、磁気素子１０は、電磁石１
１を励磁して保持体９を上方に移動させたとき
は、垂直方向になつている試験管ホルダ４の磁気
素子５に対向する位置に設けてある。 Reference numeral 9 denotes a holder with a magnetic element 10 attached to the upper part of the inner circumference.When the electromagnet 11 is excited, the holder 9 moves upward, and when the electromagnet 11 is de-energized, it is pulled back to its original position by the action of a spring 12. The magnetic element 10 is configured so that the electromagnet 1
When the holder 9 is moved upward by energizing the test tube holder 1, the holder 9 is provided at a position facing the magnetic element 5 of the test tube holder 4, which is oriented vertically.
したがつて、遠心処理後ロータ３を停止して、
試験管ホルダー４が垂直方向の位置に戻つたと
き、電磁石１１を励磁して保持体９を上方に移動
させると、磁気素子５は磁気素子１０の作用によ
り反発力を生ずるようになる。このため、この状
態で再びロータ３を回転させても、試験管ホルダ
ー４は、磁気素子５に生ずる反発力によりそのま
ま垂直方向に保持される。ただし、この場合のロ
ータ３の回転数は、磁力（反発力）＞遠心力の関
係が成立する回転数とする。これにより、試験管
６内の上澄液のみが遠心力によつて試験管６外に
放出され、上澄受け１３によつて受けられる。な
お、上澄受け１３は、ロータ３の外側で試験管６
のロに近接したところに設けてある。 Therefore, after centrifugation, the rotor 3 is stopped and
When the test tube holder 4 returns to the vertical position, the electromagnet 11 is energized to move the holder 9 upward, and the magnetic element 5 generates a repulsive force due to the action of the magnetic element 10. Therefore, even if the rotor 3 is rotated again in this state, the test tube holder 4 is held in the vertical direction by the repulsive force generated in the magnetic element 5. However, the rotational speed of the rotor 3 in this case is a rotational speed at which the relationship of magnetic force (repulsive force)>centrifugal force holds. As a result, only the supernatant liquid in the test tube 6 is discharged outside the test tube 6 by centrifugal force, and is received by the supernatant receiver 13. In addition, the supernatant receiver 13 is placed outside the rotor 3 to hold the test tube 6.
It is located near the bottom of the room.
上記した本発明の実施例によれば、赤血球を試
験管６に入れ、試験管ホルダー４に装着して、ロ
ータ３をある回転数で回転させると、試験管ホル
ダー４は遠心力で垂直方向から水平方向に移動
し、図示２点鎖線の位置に保持される。この状態
で試験管６内にノズル８から生理食塩水が注水さ
れ、赤血球と混合して洗浄を行い、引き続き遠心
処理される。その後ロータ３を停止させ、電磁石
１１を励磁して保持体９を上方に移動し、磁気素
子５に生ずる反発力によつて試験管ホルダー４を
垂直方向位置に保持し、この状態で再びロータ３
を回転させ、試験管６内の上澄液を放出させるこ
とができる。しかも、以上の操作がすべて自動的
に行われるように制御することは容易であり、こ
れにより、上記の操作を数回繰り返して、血球洗
浄を短時間に、しかも、同一条件で行うことがで
きる。特に、上澄液を放出させるとき、これを自
動的に再現性よく行うことができる。 According to the embodiment of the present invention described above, when red blood cells are put into the test tube 6, attached to the test tube holder 4, and the rotor 3 is rotated at a certain number of revolutions, the test tube holder 4 is moved from the vertical direction by centrifugal force. It moves in the horizontal direction and is held at the position indicated by the two-dot chain line in the figure. In this state, physiological saline is injected into the test tube 6 from the nozzle 8, mixed with red blood cells for washing, and then centrifuged. Thereafter, the rotor 3 is stopped, the electromagnet 11 is energized, the holder 9 is moved upward, and the repulsive force generated in the magnetic element 5 holds the test tube holder 4 in the vertical position.
can be rotated to release the supernatant in the test tube 6. Furthermore, it is easy to control the above operations so that they are all performed automatically.This allows blood cell washing to be performed in a short time and under the same conditions by repeating the above operations several times. . In particular, when releasing the supernatant, this can be done automatically and reproducibly.
以上説明したように、本発明につれば、多数の
検体を短時間の間に同一条件で洗浄処理すること
ができ、しかも、自動的に行うことができるの
で、省力化、能率向上、信頼性向上をはかること
ができるという効果がある。 As explained above, according to the present invention, a large number of specimens can be washed under the same conditions in a short period of time, and this can be done automatically, resulting in labor savings, improved efficiency, and improved reliability. It has the effect of being able to measure.
図は本発明の血球洗浄用遠心機の一実施例を示
す縦断面図である。
１……モータ、３……ロータ、４……試験管ホ
ルダー、５，１０……磁気素子、６……試験管、
７……分配素子、８……ノズル、９……保持体、
１１……電磁石、１２……ばね、１３……上澄受
け。
The figure is a longitudinal cross-sectional view showing one embodiment of a blood cell washing centrifuge according to the present invention. 1... Motor, 3... Rotor, 4... Test tube holder, 5, 10... Magnetic element, 6... Test tube,
7... Distribution element, 8... Nozzle, 9... Holder,
11... Electromagnet, 12... Spring, 13... Skim holder.
Claims (1)
ータと、該ロータ上に円形に配設され回動自在で
前記ロータ回転時に遠心力で垂直方向から水平方
向に回動する複数個の試験管ホルダーと、前記ロ
ータ回転時に前記各試験管ホルダーを所定位置に
保持するとともに前記各試験管ホルダーに装着さ
れた試験管内に血球洗浄用液体を注入する分配素
子と、前記試験管内の遠心処理後の上澄液を放出
させるときに前記試験管ホルダーを垂直方向に保
持する内周上部に磁気素子を取り付けた保持体
と、前記ロータを停止し前記試験管ホルダーが垂
直方向位置になつたときに前記試験管ホルダーに
設けた磁気素子に前記保持体に取り付けた磁気素
子が対向するように前記保持体を上方移動させて
前記試験管ホルダーを垂直方向に保持する手段と
よりなることを特徴とする血球洗浄用遠心機。1. A motor, a rotor that is connected to the motor and rotates, and a plurality of test tube holders that are rotatably arranged in a circle on the rotor and that rotate from a vertical direction to a horizontal direction due to centrifugal force when the rotor rotates. a dispensing element that holds each of the test tube holders in a predetermined position when the rotor rotates and injects blood cell washing liquid into the test tubes attached to each of the test tube holders; a holder having a magnetic element attached to the upper inner circumference for holding the test tube holder vertically when discharging the clear liquid; Blood cell washing characterized by comprising means for vertically holding the test tube holder by moving the holding body upward so that the magnetic element attached to the holding body faces a magnetic element provided on the tube holder. centrifuge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10982681A JPS5810653A (en) | 1981-07-13 | 1981-07-13 | Centrifuge for cleaning blood corpuscle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10982681A JPS5810653A (en) | 1981-07-13 | 1981-07-13 | Centrifuge for cleaning blood corpuscle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5810653A JPS5810653A (en) | 1983-01-21 |
| JPS6364744B2 true JPS6364744B2 (en) | 1988-12-13 |
Family
ID=14520185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10982681A Granted JPS5810653A (en) | 1981-07-13 | 1981-07-13 | Centrifuge for cleaning blood corpuscle |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5810653A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61139756A (en) * | 1984-12-12 | 1986-06-27 | Hitachi Koki Co Ltd | Centrifugal automatic reaction apparatus |
| DE4212006C2 (en) * | 1992-04-09 | 1994-05-11 | Molter Gmbh Dr | centrifuge |
| CN109967267B (en) * | 2019-04-17 | 2020-09-25 | 英鸿纳米科技股份有限公司 | Efficient nano-material centrifugal separation equipment |
-
1981
- 1981-07-13 JP JP10982681A patent/JPS5810653A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5810653A (en) | 1983-01-21 |
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