JPS6345210A - Sterilizing composition and sterilization - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a sterilizing composition and a sterilizing method, and more particularly to a composition that can be advantageously used in a variety of cleaning, sanitizing, and sterilizing applications.

The use of chlorine compounds in various tights cleaning, sanitizing and disinfecting compositions is well known. These chlorine compounds include, for example, chlorinated phenols such as nine-hypohydrous sodium chlorite, which was used as a wound cleansing agent during the First War of Ascension, and m-chlorophenol. Compared to non-chlorinated phenols, it has a stronger bactericidal effect but less toxicity, so the m-chlorophenol coefficient is 7.4 (B, typhosus
) and 5.8 (S, aureus).

Other chlorine compounds that have been recognized to be useful in some form of disinfection are chlorine gas itself, chlorine dioxide, chloramine T1, mercuric chloride, calcium hypochlorite (assuming there are no restrictions on their use). These include standard surface swimming pool disinfectants), chlorapicrin (pest control agent), chloroform (fumigation disinfectant), chloradan (insecticide), zinc chloride (preservative) and chloromycetin (antibiotic).

Chlorine dioxide, in particular, is known to be an effective disinfectant. This compound is extremely versatile and is used as a bleaching agent, for example in the oxidative bleaching of natural colorants present in cotton, wood pulp and other cellulosic fibrous materials, even though it performs an oxidizing function. Harmless with respect to FR male material.

Additionally, chlorine dioxide has long been used in the treatment of water supplies and is currently commercially available as a powder for swimming pools and as a liquid for cleaning and disinfecting homes and industries. In general, chlorine dioxide is superior to gaseous chlorine in eliminating odors and tastes, and in destroying and removing foreign and other organic substances, and is superior to chlorine gas as a disinfectant, virucidal, or sporicidal agent. It is believed to be at least as effective, if not more effective, and is also less sensitive to pH than chlorine in terms of preservative properties. Thus, chlorine dioxide has the advantage of retaining its disinfecting ability to a much greater degree than chlorine gas and over a wider pH range.

However, despite the many advantages afforded by the use of chlorine dioxide as described above, some difficulties are still encountered in practice.

For example, chlorine dioxide, as a concentrated gas, is explosive and toxic, and intermediate gases are usually not transported in gaseous form to small volume users. Therefore, saving M
, safe transport and handling) IJ
It is common to use compounds that liberate chlorine dioxide, such as chlorine powder. Chlorine dioxide is usually generated from the substance by one of the following three methods.

(1) Addition of acid H"+NaC402HC202+Na"5HC-C
O24CJ 02+HC,! +2H20 (2) Bleach (
Addition of hypochlorous acid group) 2NaC-! 02+NaC10+H2O2CJOz+N
aOH+NaCJ' (3) Addition of chlorine 2NaCJO2+C422CJO2+2NaCJ chlorine dioxide, which is also referred to as CjQz from now on, the reaction (1
) is also generally caused by relatively inexpensive inorganic acids such as hydrochloric acid and sulfuric acid. For home use, phosphoric acid or acetic acid (vinegar) are sometimes indicated, since these acids are relatively safe to handle and are generally readily available.

Conventional acid generation of C2O4 from sodium chlorite hitherto estimated and practiced has proven to be largely ineffective. For example, it has often been found that the acidic substances used thereby tend to be released and react with CJQz, reducing the effective amount of the active ingredients available for useful purposes.

Furthermore, compositions derived from acidification of sodium chlorite usually do not exhibit the desired disinfecting effect, especially in terms of disinfection rate. In order to compensate for this drawback, sodium chlorite IJ
It becomes necessary to increase the concentration of umum and acid, which can lead to toxicity problems, especially when the composition is used in an enclosed space.

Another problem arises from the fact that the compositions resulting from the interaction of sodium chlorite and acidic substances do not provide a truly effective solvent for by-products containing active chlorine such as C2O4 and chlorite. .

Inhalation of these gaseous components to some extent is, of course, harmful to health, and therefore the risk to personal safety becomes an important issue. Admittedly, this toxicity problem places severe limitations on the general use of fungicidal compositions, particularly for human treatment.

The present inventor conducted various studies and experiments to overcome the above-mentioned drawbacks, and found that by selecting lactic acid from various acidic substances that react with chlorite, an excellent sterilization effect could be obtained. Based on this knowledge, the present invention has been made based on this knowledge and has a high degree of bactericidal effect against bacteria, bacteria-producing organisms, and other harmful substances, stably under a wide range of conditions. It also minimizes the loss of active ingredient due to fugitive side reactions, and has negligible toxicity under the conditions under which its use is normally indicated, and is therefore suitable for food storage, rutens, etc.
The present invention provides a sterilizing composition and a sterilizing method that are extremely useful in connection with the sterilizing treatment of various types of wounds, including medical burdock, human anatomy, and other types of wounds.

The foregoing and related aspects are obtained by the present invention, which in its broader sense provides a germicidal composition and a method for calibrating the composition, which method comprises adding sodium chlorite to the composition. , with an essentially water-soluble acidic substance selected from the group consisting of organic acids and mixtures thereof with inorganic acids, the acidic substance having at least 15
1 percent by weight of lactic acid, and the contacting is in an aqueous medium and in the presence of an amount of nucleic acid sufficient to lower the PHt of the aqueous medium to about 7 or less.

Further in this embodiment, the invention provides that either the germicidal composition itself or the reactants capable of producing it in situ,
A method of cleaning, sanitizing, and disinfecting is provided that utilizes the composition, including its use in closed, cubical spaces as well as bacterial carriers containing various types of materials.

The use of lactic acid in the compositions and methods provided herein is significant. For example, this particular compound is known to work synergistically with sodium chlorite under the aforementioned conditions to provide a highly effective disinfectant composition. This result is somewhat surprising. This is because lactic acid normally reacts with the resulting by-product chlorine dioxide, as is the case with some similar acid compounds, to eliminate the available lactic acid and chlorine dioxide that would otherwise serve a useful purpose. This is because it is thought to reduce the Also, the lactate produced during this reaction would normally be considered an impurity during bleaching operations, water sterilization, and organic matter removal.

However, it is clear that in the present invention, such undesirable side reactions, as well as the attendant deleterious effects, are negligible, if any at all.

To prepare this composition, lactic acid is contacted with sodium chlorite in an aqueous medium, and the acid is used in an amount sufficient to lower the pH of the composition to about 7 or less. Water supply requirements are usually met by this acid and chlorite (both of these substances are utilized in varying concentrations in aqueous solution. The relative proportions of chlorite compounds and lactic acid are The pH inside is about 7
Choose from the following. The amount of acid required is of course determined in part by the strength of the acid reagent solution as well as the total dilution of the acid and chlorite compositions respectively at the time of mixing and fc.

However, the required amount can be determined in advance more easily in a reasonable manner.

The disinfectant composition obtained by contacting or reacting chlorite with lactic acid comprises a mixture comprising chlorine dioxide, chlorite, lactic acid and sodium lactate.

Equilibrium mixtures of the aforementioned substances are believed to exist in the form of complex compounds, and analysis shows the presence of at least the components listed above. This mixture or complex compound is relatively stable. However, for optimal bactericidal efficacy, the composition should be used within a period of up to about 48 hours after its preparation. However, if lactic acid and chlorite are separated from each other by separate packaging,
This could be done using a single or common container, but
In this case, if contact between chlorite and lactic acid materials is prohibited until the time of full use, there is almost no restriction on the period of exposure.

Therefore, the chlorite and lactic acid ingredients are all lightly pressed with the fingers and are equipped with a pulp-type dispensing means that can be moved by just using the air.

The chlorite and lactic acid components may be mixed and released in a thin spray at nearly the same time by being confined in separate container compartments in a sol type. The required aerosol pressure is determined by the hydrocarbon and/or halogenated, e.g. chlorinated,
It can be supplied by known propellant gases including fluorinated tertiary hydrocarbons. The amount of propellant gas used must be such as to cause essentially complete displacement or evacuation of the contents of the aerosol container. Container constructions useful in this regard are in any case known in the art.

Alternatively, the chlorite and lactic acid could be packaged separately and sold as a unit with appropriate instructions for mixing and use by the consumer at home.

This composition product is advantageous in many ways. For example 1, without any necessary restrictions, S, aureus, S, alb
us.

psuedomonas, E, colt, Pro
teusvulgaris.

streppyogen*s, Candida a
lbicans (dried) spores, B, 5ubtilus
The remarkable bactericidal properties of this composition with respect to bacteria, including (dry) spores, etc., are particularly due to the low toxic concentration of this composition as well as the low temperature at which it is used for sterilization (approximately 50°C). I hope it proves to be amazing.

Furthermore, the tested microorganisms were completely killed within about 10 minutes when using tap water at 50°C, and within 5 minutes when using this composition in an ultrasonic cleaner.

Extensive testing has confirmed that the composition is less irritating and less toxic than, for example, white flour. The latter has long been considered relatively harmless locally. In general, solutions of ctoz should be considered non-hazardous and are routinely poured into rivers and the like by textile processors without harmful consequences. In fact, such solutions are commonly used to clean fish stocks in public and domestic fish ponds. Further cto
Z is used as a preservative for various foods, including cottage cheese, and is also used to sanitize food containers, so that they do not need to be washed after processing. The compositions are even less toxic than conventional CTO2 antiseptic solutions, and therefore can be used effectively in the treatment of open wounds, cleaning surgeons' hands, and the like, as well as those mentioned above.

When used as a wound cleanser, it has often proven advantageous to use a buffering agent to help maintain a suitable pH.

Without wishing to be bound by any theory, the following is self-evident in explaining the highly effective microbicidal properties provided by the compositions of the present invention. For example, ctoz is about five times more soluble in water than chlorine, and therefore appears to be much less likely to be lost to volatilization. Additionally, chlorite ions are significantly less corrosive than hypochlorite ions;
For example, in the case of fabric bleaching, the presence of C6O2 is sufficient to protect the fabric from the degrading effects of hypochlorite. Bleach's disinfecting power is generally due to its biocidal action, which is derived from the reaction between hypochlorous acid and the enzyme triphosphate dehydratase, as it diffuses through cell walls and reaches the vital points of bacteria. Other authorities believe that C2O2 accelerates bacterial cell metabolism and damages cell growth. Other reputable authorities claim that the chloride ions in ClO2 pass through eight possible oxidation states as they pass through the spore wall.

As an algaecide, ClO2 destroys chlorophyll, decomposes cells, causing water to be lost from the protoplasm, and then completely destroys or oxidizes the cells, leaving no muddy residue on the water filter. The presence of lactic acid appears to enhance or augment the aforementioned mechanisms.

For example, lactic acid, a natural by-product of muscle work and widespread bacterial fermentation, is not foreign to the bacterial environment like other and closely related acids. In this regard, Lactic acid is much better able to penetrate the bacterial cell wall without being "rejected," and in doing so carries with it ClO2 or chlorite molecules.Once it penetrates the bacterial cell, It is very likely that lactic acid and its salts can influence the metabolic activity of cells and produce intermediate compounds which are particularly susceptible to the bactericidal action of ClO2 or its derived products. These or ClO2 may also inactivate enzymes in critical metabolic processes by promoting the formation of stable chlorine intermediates.Moreover, the oxidative effect of the excess chloride ions on the outside of the cell wall It may also be enhanced by the lactic acid coating.

Notwithstanding the foregoing, it is determined by the present invention that lactic acid can be added to particularly chlorite materials to produce efficacious germicidal compositions.

This germicidal composition is particularly effective when used in ultrasonic cleaner devices. The bactericidal properties of ultrasound alone or cavitation have been studied for many years.

If the intensity is high enough, for example over 100 watts per d, cavitation will not only kill all cells but also destroy them. However, in conventional ultrasonic cleaners, the intensity is 1
It is much smaller on the order of Watsuto. However, at this intensity level, bacteria will grow at a faster rate than without sonication due to the separation of bacterial clumps and grains. However, the use of this germicidal composition in ultrasonic cleaner devices at low or normal levels of intensity has proven to be much more effective than previously used germicidal compositions. Therefore, as well as being odorless, the composition is much less toxic, less polluting, and more effective at lower temperatures. Use of the present compositions in ultrasonic cleaner devices allows surgeons, dentists, food processors, and others to quickly both clean and sterilize instruments and equipment in a single operation. It is believed that such usage will break up the bacterial clumps, break them up, and remove them from the utensils and equipment, exposing them completely to the solution and its disinfecting effects. It will be done. Often the microscopic bubbles that surround and otherwise protect the bacteria are destroyed. These bubbles are filled with ctoz similar to bubbles produced by cavitation. These very small bubbles are often found in scratches, small cracks and other defects in instruments and equipment being cleaned (5!K).
<Poke to ensure sufficient sterilization.

Additionally, cavitation causes the disinfectant composition to attack the cell walls of the bacteria, thereby facilitating diffusion of the disinfectant composition into the interior of the bacteria.

Therefore, according to the present invention, the combination of the inherent advantages of ultrasonic cleaner devices and the excellent bactericidal properties of the present composition makes it possible to eliminate various substances that provide a place for the accumulation of bacteria, viruses, spores, etc. A ninth effective method of cleaning, sanitizing and disinfecting is provided.

Using lactic acid alone in combination with chlorite substances
This constitutes a particularly preferred embodiment of the invention. However, it is also effective to use lactic acid in combination with other acids, including organic or inorganic acids. An appropriate organic number is
Examples include water-soluble or water-dispersible monocarboxylic and polycarboxylic acids containing gold and having 2 to about 16 carbon atoms, such as acetic acid, citric acid, sorbic acid, fumaric acid, and tannic acid.

Suitable inorganic acids include, for example, sulfuric acid, hydrochloric acid, phosphoric acid, and the like. When using acid mixtures, to ensure effective results, lactic acid should account for at least about 15 weight percent of the total mixture, preferably at least about 45 weight percent.

Although the use of sodium chlorite is preferred as the ctoz releasing agent, other water-soluble cations can also be used in place of sodium, including other alkali metals and alkaline earth metals such as potassium. However, the former is particularly preferred.

As used herein, the terms ``substrate'' and ``bacterial carrier'' refer to bacteria,
Viruses, spores, bacteria, fungi, all tight microorganisms. collection,. Place □ offer, get, su, a.

type of hard surface or carrier. Obvious examples include surgical and dental instruments, food containers, human tissue, swimming pools, household sinks, garbage containers, bathroom utensils, etc. The cleaning action is facilitated by adding a wetting agent, the latter being compatible with the cleaning action and free from any tendency to react with ClO2. A particularly effective wetting agent for use in this manner is Du pon
There are commercially available fluorocarbon surfactants available from T.T. The aerosol type of the composition is effectively used for the destruction of airborne or atmospheric bacteria such as those carried in confined spaces. “Bacterial carrier germ c” used here
The term "arrier" refers to such an atmospheric, gaseous carrier.

In instances where the antiseptic composition is used on human tissue as a wound cleanser, a buffering agent may be included to maintain the pH compatible with such tissue. Conventional buffering agents such as alkali metal bicarbonates are used for this purpose.

The composition can be used in a relatively wide range of concentrations;
The fundamental requirement is that at least the entire effective germicidal dose should be used, even in small amounts. The upper limit of the amount used is often determined by the fact that no further advantageous effects can be achieved beyond this amount. The required surface activity in a particular case is also influenced by factors such as temperature and certain types of spectral radiation that cause loss of ClO2 from the solution. However, in general,
Effective disinfection results are obtained using chlorite compounds in amounts ranging from about 100 to 5000 ppm, preferably at concentrations of about 2700 to 3300 ppm in solution.

The following examples are given by way of illustration only and are not to be considered as limiting the invention.

All parts and percentages are by weight unless otherwise noted.

Example 1 To an aqueous solution of sodium chlorite containing 3000 ppm of sodium chlorite is added an aqueous lactic acid solution in an amount sufficient to lower the PHt of the resulting solution to approximately 3. When a sample is taken and analyzed, it is found that it is composed of chlorine dioxide, chlorous acid, lactic acid, and sodium lactate.
(approximately 50° C.) and test the following: Le.

a) S, aureus b) S, albus c) Psuedomonas d) E, coli e) prot@us vulgarisf) 5
trep Pyogenesg) (andida
Albieiua (dried) spore h) B, 5ub
Tilus (dried) spores tested on each side and confirmed bacteria sample pan cylinder and 5urica
Immerse it in the l knot until it is saturated. The test specimens were then immersed in a sterilizing composition prepared as described above. In each case, the microorganisms were completely killed in about 10 minutes.

Example 2 Example 1 was repeated nine times, but the test was conducted at room temperature using an ultrasonic cleaner device with an intensity of 1 watt per minute. In this case, the tested microorganisms were completely killed within 5 minutes.

The foregoing results are particularly surprising in view of the generally low temperatures during testing and the relatively low level of toxicity of this germicidal composition.

Repeating the previous example, nine times the lactic acid was replaced with (a) phosphoric acid, (b) acetic acid, (c) sorbic acid, (d) fumaric acid, (
,) sulfamino acid, (f) succinic acid, (g) boric acid,
When substituting (h) tannic acid and (i) citric acid, the results obtained were significantly inferior, in terms of speed of sterilization and completeness of sterilization, to the results obtained with lactic acid.几. Again, this result is somewhat surprising in view of the close relationship between some of the acids tested and lactic acid.

Examples 1 and 2 were repeated, but a portion of the lactic acid was replaced with up to about 80% of each of phosphoric acid, acetic acid, sorbic acid, etc., and an effective sterilizing composition was obtained, but with improved sterilization. The effects were not as significant as those characterizing the compositions of Examples 1 and 2.

Procedural amendment September 7, 1988 Director General of the Patent Office Kunio Ogawa 1. Patent application filed on August 12, 1986 2. Title of invention: Sterilizing composition and sterilizing method 3 , Relationship with the case of the person making the amendment Patent applicant Name: Howard Alliger 4, Agent: 101 Address: 6th floor, Toko Building, 1-15-16 Uchikanda, Chiyoda-ku, Tokyo Telephone: 295-1480 Scope of claims A sterilizing composition.

A sterilization method comprising contacting an effective sterilization amount of a total sterilization composition with a sterilization carrier.

(3) The sterilization method according to claim 2, wherein the contact with the bacterial carrier is carried out by dispersing and zesting the sterilizing composition from a pressurized aerosol container equipped with a valve dispensing means.

(4) The sterilization method according to claim 2, wherein the contact with the bacterial carrier is carried out by introducing the sterilization composition into an ultrasonic cleaning device.

(5) The sterilization method according to claim 2, wherein the contact with the bacterial carrier is carried out by dispersing the sterilizing composition using an ultrasonic spray generator.

Claims (7)

[Claims] (1) contacting chlorite in an aqueous medium with an acidic substance selected from the group of inorganically water-soluble organic or inorganic acids containing at least 15 percent by weight of lactic acid;
A sterilizing composition characterized in that the acidic substance is contained in an amount sufficient to adjust the pH of the aqueous medium to about 7 or less. (2) The sterilizing composition according to claim 1, wherein the acidic substance consists of lactic acid only. (3) reacting chlorous acid or a salt thereof with an essentially water-soluble acidic substance selected from the group of organic or inorganic acids, the acidic substance containing at least 15 weight percent lactic acid; This contacting is carried out in an aqueous medium in the presence of an amount of the above acidic substance sufficient to bring the pH of the aqueous medium to about 7 or less, thereby bringing the bacterial carrier into contact with an effective sterilizing amount of the sterilizing composition obtained. A sterilization method characterized by: (4) The sterilization method according to claim 3, wherein the acidic substance is only lactic acid. (5) The sterilization method according to claim 3, wherein the contact with the bacterial carrier is carried out by dispersing the sterilizing composition from a pressurized Niasol container equipped with a valve dispensing means. (6) The sterilization method according to claim 3, wherein the contact with the bacterial carrier is carried out by introducing the sterilizing composition into an ultrasonic cleaning device. (7) The sterilization method according to claim 3, wherein the contact with the bacterial carrier is carried out by dispersing the sterilizing composition using an ultrasonic spray generator.