JPS6345059B2 - - Google Patents
Info
- Publication number
- JPS6345059B2 JPS6345059B2 JP55162655A JP16265580A JPS6345059B2 JP S6345059 B2 JPS6345059 B2 JP S6345059B2 JP 55162655 A JP55162655 A JP 55162655A JP 16265580 A JP16265580 A JP 16265580A JP S6345059 B2 JPS6345059 B2 JP S6345059B2
- Authority
- JP
- Japan
- Prior art keywords
- ion
- selective
- measured
- flow path
- selective electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 150000002500 ions Chemical class 0.000 claims description 37
- 229910001414 potassium ion Inorganic materials 0.000 claims description 15
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- -1 halogen ions Chemical class 0.000 claims description 8
- 229910052736 halogen Inorganic materials 0.000 claims description 6
- 238000011144 upstream manufacturing Methods 0.000 claims description 6
- 108010067973 Valinomycin Proteins 0.000 claims description 4
- 125000005210 alkyl ammonium group Chemical group 0.000 claims description 4
- FCFNRCROJUBPLU-UHFFFAOYSA-N compound M126 Natural products CC(C)C1NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC(=O)C(C(C)C)NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC(=O)C(C(C)C)NC(=O)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)C)OC1=O FCFNRCROJUBPLU-UHFFFAOYSA-N 0.000 claims description 4
- FCFNRCROJUBPLU-DNDCDFAISA-N valinomycin Chemical compound CC(C)[C@@H]1NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC(=O)[C@H](C(C)C)NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC(=O)[C@H](C(C)C)NC(=O)[C@H](C)OC(=O)[C@@H](C(C)C)NC(=O)[C@@H](C(C)C)OC1=O FCFNRCROJUBPLU-DNDCDFAISA-N 0.000 claims description 4
- 239000000460 chlorine Substances 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 3
- 230000006866 deterioration Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/4166—Systems measuring a particular property of an electrolyte
Description
【発明の詳細な説明】
本発明はフローセル中に配置したイオン選択性
電極を用いたイオン濃度の測定方法に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for measuring ion concentration using an ion-selective electrode placed in a flow cell.
イオン選択性電極は、被測定液中に浸漬したと
き、その測定電位が測定対象イオンの活量の対数
に比例するので、液中の特定イオンの濃度のモニ
タ、水質分析など広い分野で用いられている。と
くに、最近ではこれを医療用、とりわけ血液中に
存在するNa+、K+、Cl-などのイオンの定量に用
いる試みが盛んになつている。 When an ion-selective electrode is immersed in a liquid to be measured, its measurement potential is proportional to the logarithm of the activity of the ion to be measured, so it is used in a wide range of fields, such as monitoring the concentration of specific ions in liquids and analyzing water quality. ing. In particular, recently there have been many attempts to use this method for medical purposes, particularly for the determination of ions such as Na + , K + , and Cl - present in blood.
一方、最近、被測定液中に存在する複数個の異
種測定対象イオンをそれぞれを選択的に検出する
複数個のイオン選択性電極を用いて同時に定量す
る試みがなされている。このような同時測定にお
いて、あるイオン選択性電極がその構成部材（主
としてイオン選択性膜）から被測定液に溶出して
他のイオン選択性電極の検出能を妨害する成分を
含む場合には、他のイオン選択性電極により検出
されるイオン濃度の誤差を大たらしめるのみなら
ず、その使用寿命を著るしく短かくするという問
題がある。 On the other hand, recently, attempts have been made to simultaneously quantify multiple ion-selective electrodes that selectively detect a plurality of different target ions present in a liquid to be measured. In such simultaneous measurements, if one ion-selective electrode contains a component that elutes from its constituent members (mainly the ion-selective membrane) into the sample liquid and interferes with the detection ability of other ion-selective electrodes, This poses a problem in that it not only increases the error in the ion concentration detected by other ion-selective electrodes, but also significantly shortens its service life.
例えば、血液中のK+とCl-を同時に測定しよう
とする場合に、該血液被測定液中に、バリノマイ
シンを含むカリウムイオン選択性膜を有するカリ
ウムイオン選択性電極と第四級アルキルアンモニ
ウム塩（例えばトリメチルカプリルアンモニウム
塩又はジメチルジステアリルアンモニウム塩）を
含む塩素イオン選択性膜を有する塩素イオン選択
性電極を同時に挿入してそれぞれのイオン濃度を
測定すると、塩素イオン選択性膜から溶出する第
四級アルキルアンモニウム塩によつてカリウムイ
オン選択性膜が劣化し、カリウムイオン選択性電
極のK+に対する選択的な検出能が低下しかつそ
の使用寿命が著るしく短かくなる。 For example, when attempting to simultaneously measure K + and Cl - in blood, a potassium ion-selective electrode having a potassium ion-selective membrane containing valinomycin and a quaternary alkyl ammonium salt ( For example, when a chloride ion-selective electrode with a chloride ion-selective membrane containing (for example, trimethylcaprylammonium salt or dimethyldistearylammonium salt) is inserted at the same time and the respective ion concentrations are measured, quaternary Alkylammonium salts degrade the potassium ion-selective membrane, reducing the selective detection ability of the potassium ion-selective electrode for K + and significantly shortening its service life.
とくに、被測定液を通流しながら、その流路に
異種の測定対象イオンをそれぞれ選択的に検出す
る複数個のイオン選択性電極を同時に配置して該
フローセル中の各イオン濃度を測定するフローセ
ルを用いる方法においては、他のイオン選択性電
極を劣化させる溶出成分を含むイオン選択性電極
をその流路の上流に配置した場合、下流に配置さ
れたイオン選択性電極の検出能が劣化してその使
用寿命が著るしく短かくなる。 In particular, a flow cell is used that measures the concentration of each ion in the flow cell by simultaneously disposing a plurality of ion-selective electrodes for selectively detecting different types of ions to be measured in the flow path while flowing the liquid to be measured. In the method used, if an ion-selective electrode containing an eluted component that degrades other ion-selective electrodes is placed upstream of the flow path, the detection ability of the ion-selective electrode placed downstream will deteriorate and the The service life will be significantly shortened.
本発明は、被測定液中の各種測定対象イオンを
同時測定する場合、とりわけフローセルを用いて
同時測定する場合の上記のような不都合を解消
し、イオン選択性電極の劣化を防止してその使用
寿命を大たらしめるイオン濃度の測定方法の提供
を目的とする。 The present invention solves the above-mentioned inconveniences when simultaneously measuring various target ions in a liquid to be measured, especially when simultaneously measuring using a flow cell, and prevents deterioration of the ion-selective electrode and facilitates its use. The purpose is to provide a method for measuring ion concentration that increases lifespan.
本発明方法は、フローセルの流路に被測定液を
通流し、該流路にカリウムイオンおよびハロゲン
イオンを含む異種の測定対象イオンを選択的に検
出する複数個のイオン選択性電極を同時に配置し
て該被測定液中のイオン濃度を測定する方法にお
いて、該流路の上流にはバリノマイシンを含むカ
リウムイオン選択性電極、下流には第四級アルキ
ルアンモニウム塩を含むハロゲンイオン選択性電
極を配置して各イオン濃度を測定することを特徴
とするものである。 In the method of the present invention, a liquid to be measured is passed through a flow path of a flow cell, and a plurality of ion-selective electrodes are simultaneously arranged in the flow path to selectively detect different types of ions to be measured, including potassium ions and halogen ions. In this method, a potassium ion-selective electrode containing valinomycin is placed upstream of the flow path, and a halogen ion-selective electrode containing a quaternary alkyl ammonium salt is placed downstream of the flow path. This method is characterized by measuring the concentration of each ion.
本発明方法において例えば、フローセルの流路
に流れる被測定液中に５種の測定対象イオンが存
在しており、各々のイオン濃度を５本のイオン選
択性電極Ａ、Ｂ、Ｃ、Ｄで測定し、Ａがカリウム
イオン選択性電極、Ｂがハロゲンイオン選択性電
極である場合、該流路の上流にＡをそれより下流
にＢを配し、他のＣ、ＤおよびＥはどこに配置し
てもかまわない。そうすることによつて、ＡはＢ
からの溶出成分により劣化作用を受けることがな
い。 In the method of the present invention, for example, five types of ions to be measured are present in the liquid to be measured flowing through the flow path of a flow cell, and the concentration of each ion is measured using five ion-selective electrodes A, B, C, and D. However, when A is a potassium ion selective electrode and B is a halogen ion selective electrode, A is placed upstream of the flow path, B is placed downstream from it, and where are the other C, D and E placed? I don't mind. By doing so, A becomes B
It is not affected by deterioration due to components eluted from.
本発明方法において、各イオン選択性電極を配
置する際に、劣化作用を及ぼすハロゲンイオン選
択性電極と劣化作用を受けるカリウムイオン選択
性電極との隣接する間隔は少なくとも0.2cmであ
ることが望ましくこの間隔があまりに小さいと、
溶出成分の流れている被測定液中上流への拡散を
無視できなくなる。 In the method of the present invention, when arranging each ion-selective electrode, it is preferable that the adjacent spacing between the halogen ion-selective electrode, which exerts a deteriorating effect, and the potassium ion-selective electrode, which undergoes a degrading effect, be at least 0.2 cm. If the interval is too small,
Diffusion of eluted components upstream in the flowing sample liquid cannot be ignored.
以下、本発明方法を実施例に基づいて説明す
る。 The method of the present invention will be explained below based on examples.
実施例
図に例示したフローセルを用いてK+とCl-のイ
オン濃度を連続的に測定した。Example The ion concentrations of K + and Cl - were continuously measured using the flow cell illustrated in the figure.
図において、１はフローセルでこの中にK+と
Cl-を含む被測定液が流れている。２はフローセ
ル１の流路に２cmの間隔を置いて設けられたイオ
ン選択性電極のホルダーである。３はバリノマイ
シンを含むカリウムイオン選択性膜を有するカリ
ウムイオン選択性電極、４はメチルトリカプリル
アンモニウムクロライドを含む塩素イオン選択性
膜を有する塩素イオン選択性電極でそれぞれはホ
ルダー２に挿入されて先端は図の左から右方向へ
通流する被測定液（従つて左側が上流）中に浸漬
されている。５は参照電極としての銀−塩化銀電
極である。各電極からは電気信号を取り出すリー
ド線が引き出され、インピーダンス変換の演算増
幅器６及び電圧計７に接続されている。８は、
K+、Cl-の測定用の切換えスイツチである。 In the figure, 1 is a flow cell in which K + and
A liquid to be measured containing Cl - is flowing. Reference numeral 2 denotes a holder for ion-selective electrodes provided in the flow path of the flow cell 1 at an interval of 2 cm. 3 is a potassium ion selective electrode having a potassium ion selective membrane containing valinomycin, and 4 is a chloride ion selective electrode having a chloride ion selective membrane containing methyltricaprylammonium chloride. It is immersed in a liquid to be measured that flows from left to right in the figure (therefore, the left side is upstream). 5 is a silver-silver chloride electrode as a reference electrode. Lead wires for extracting electrical signals are drawn out from each electrode and connected to an operational amplifier 6 for impedance conversion and a voltmeter 7. 8 is
This is a switch for measuring K + and Cl - .
このような装置でK+、Cl-を連続的に測定した
ところカリウムイオン選択性電極３は殆んど劣化
せず６ケ月以上の使用寿命を保つた。 When K + and Cl - were continuously measured using such an apparatus, the potassium ion selective electrode 3 showed almost no deterioration and had a service life of more than 6 months.
逆に電極３と電極４の位置を入れ換えて同様に
して連続的な測定を行なつたところ、カリウムイ
オン選択性電極３の使用寿命は約１週間と極端に
短かくなつた。 Conversely, when the positions of electrode 3 and electrode 4 were changed and continuous measurements were performed in the same manner, the service life of potassium ion selective electrode 3 was extremely short to about one week.
以上の結果から明らかなように、本発明の測定
方法はイオン選択性電極の使用寿命を長期化する
ことができて有用である。 As is clear from the above results, the measurement method of the present invention is useful because it can extend the service life of the ion-selective electrode.
図は、本発明測定方法の効果を示すためのフロ
ーセルを用いたK+、Cl-のイオン濃度の測定系で
ある。
１……フローセル、２……ホルダー、３……カ
リウムイオン選択性電極、４……塩素イオン選択
性電極、５……銀−塩化銀電極（参照電極）、６
……演算増幅器、７……電圧計、８……切換えス
イツチ。
The figure shows a system for measuring K + and Cl - ion concentrations using a flow cell to demonstrate the effects of the measuring method of the present invention. 1... Flow cell, 2... Holder, 3... Potassium ion selective electrode, 4... Chlorine ion selective electrode, 5... Silver-silver chloride electrode (reference electrode), 6
... operational amplifier, 7 ... voltmeter, 8 ... changeover switch.
Claims (1)
路にカリウムイオンおよびハロゲンイオンを含む
異種の測定対象イオンを選択的に検出する複数個
のイオン選択性電極を同時に配置して該被測定液
中のイオン濃度を測定する方法において、 該流路の上流にバリノマイシンを含むカリウム
イオン選択性電極、下流には第四級アルキルアン
モニウム塩を含むハロゲンイオン選択性電極を配
置することを特徴とするイオン濃度の測定方法。[Claims] 1. A liquid to be measured is passed through a flow path of a flow cell, and a plurality of ion-selective electrodes are simultaneously installed in the flow path to selectively detect different types of ions to be measured, including potassium ions and halogen ions. A method for measuring the ion concentration in the liquid to be measured by arranging a potassium ion-selective electrode containing valinomycin upstream of the flow path, and arranging a halogen ion-selective electrode containing a quaternary alkyl ammonium salt downstream of the flow path. A method for measuring ion concentration characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55162655A JPS5786750A (en) | 1980-11-20 | 1980-11-20 | Measuring method for ion concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55162655A JPS5786750A (en) | 1980-11-20 | 1980-11-20 | Measuring method for ion concentration |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2126961A Division JPH03205546A (en) | 1990-05-18 | 1990-05-18 | Instrument for measuring ion concentration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5786750A JPS5786750A (en) | 1982-05-29 |
| JPS6345059B2 true JPS6345059B2 (en) | 1988-09-07 |
Family
ID=15758747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55162655A Granted JPS5786750A (en) | 1980-11-20 | 1980-11-20 | Measuring method for ion concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5786750A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH662423A5 (en) * | 1982-07-27 | 1987-09-30 | Inst Geol Im Akademika I M Gub | GAS AND LIQUID ANALYZER. |
-
1980
- 1980-11-20 JP JP55162655A patent/JPS5786750A/en active Granted
Non-Patent Citations (1)
| Title |
|---|
| BLOMEDICAL JOURNAL=1978 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5786750A (en) | 1982-05-29 |
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