JPS6329236Y2 - - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to a reaction cell device, and more specifically, the present invention relates to a reaction cell device, in which a desired amount of a plurality of liquids is accurately measured and mixed, a reaction is caused if necessary, and then the mixed liquid or reaction liquid is The present invention relates to a reaction cell device that can perform all operations such as measuring the transmittance of a cell.

In the field of analysis, mixing, dispensing, and reaction operations, in which multiple liquids are measured, mixed, and reacted, are important not only in manual methods, but also in automatic analyzers. Particularly in the case of colorimetric analysis using an automatic analyzer, it is common practice to develop a color in the sample solution by the above procedure, and then introduce this coloring solution into a cell, where its transmittance is measured. The equipment used to do this has become complex. For example, three pumps are used to mix the sample solution with predetermined amounts of two types of reagents to cause the sample to develop color, and then introduce the colored sample into a cell where the transmittance is measured. A predetermined amount of the liquid and reagent are sent to a mixing tank, and the three components are mixed and reacted in the mixing tank to develop a color.The coloring solution is then sent to a cell using another pump, where its permeation is carried out. Generally, the ratio is measured, and in this case, four pumps, a mixing tank, a cell, a control unit for controlling these, etc. are required, resulting in a complicated configuration. As described above, since automatic analyzers require a large number of component units, errors in mixing, dispensing, etc. often become a problem, and furthermore, errors due to various complicated operations such as reaction and transfer to the detection section, etc. Since errors are added and cause a decrease in analysis accuracy, it has been strongly desired to perform the above operations with high precision and to simplify the constituent units.

The present invention was developed in view of the above circumstances, and by using a special reaction cell device and equipping it with many functions, it reduces the number of constituent units, minimizes the factors that degrade accuracy, and improves operation. The purpose of the present invention is to provide a reaction cell device with improved stability and maintainability.

Hereinafter, one embodiment of the present invention will be described with reference to the drawings.

The drawing shows an embodiment of the reaction cell device of the present invention, and in the drawing, 1 is a cylinder. This cylinder 1
A light receiving section 2 consisting of a photodiode or the like is embedded in approximately the center of the bottom wall of the cylinder 1, with its light receiving surface aligned with the top surface of the bottom wall of the cylinder 1, and the signals generated by this light receiving section 2 are connected to this. The signal is taken out from the signal leader line 3. Further, at a predetermined height of the side wall of the cylinder 1, as shown in FIG. are formed sequentially.
One end of the first to third liquid suction pipes 6a to 6c is connected to the first to third suction parts 4a to 4c, respectively, and the other end is connected to the first to third liquid tanks 7a to 7a.
It is immersed in the first to third liquids 8a to 8c in 7c. Similarly, one end of the discharge pipe 9 is connected to the discharge part 5, and the other end is connected to the receiver 1.
It is inserted within 0.

Inside the cylinder 1 is a cylindrical plunger 11.
The lower side of the holder is inserted in a liquid-tight manner so that it can slide and rotate freely. A notched groove 12 reaching from a predetermined height to the lower end face is formed in a part of the surface of the lower part of the plunger 11, and a light receiving surface of the light receiving part 2 is formed approximately in the center of the lower end of the plunger 11. A light emitting section 13 such as a tungsten lamp is embedded opposite to the plunger with its light emitting surface aligned with the lower end surface of the plunger, and the light emitted by the light emitting section 13 is detected by the light receiving section. Note that 14 is a lead wire connected to the light emitting section 13, and is connected to the plunger 11.
The lead wire 14 passes through the inside and is drawn out to the outside from a predetermined location, and power is sent to the light emitting section 13 via this lead wire 14 to cause the light emitting section 13 to emit light. Also,
A plurality of tooth grooves are formed along the entire circumference in the circumferential direction in the longitudinally intermediate portion of the plunger 11,
There are 15 round racks.

Reference numeral 16 denotes a pulse motor whose rotating direction and angle of rotation are controlled by pulse signals sent from a control section 17. A pinion 18 attached to the rotating shaft of this motor 16 is connected to the round rack 15. When engaged, the rotational motion of the motor 16 is converted into linear motion and transmitted to the plunger 11. In this case, since tooth grooves on the rack are formed over the entire circumference, the plunger 11 rotates around its axis. However, the state of engagement is always maintained.

A plunger rotation pulse motor 19 whose rotation direction and rotation angle are controlled by pulses sent out from the control section 17 is disposed above the plunger 11, and the rotation axis of this motor 19 is formed by a spline or the like. is connected to the plunger 11 by a connecting structure (not shown), whereby the plunger 11
is rotated around its axis.

Next, to explain the case where the sample is colored using the reaction cell device and the target component in the sample is quantified by colorimetric analysis, first, the plunger 11 is inserted into the cylinder 1 to the maximum extent, and the bottom of the cylinder 1 is With the lower end of the plunger 11 in contact with the wall,
The amount of sample and reagent to be inhaled, reaction time, etc. are set in the control unit 17. Further, the first liquid tank 7a is filled with a sample solution, and the second and third liquid tanks are filled with a PH buffer and a coloring reagent, respectively. Next, when the analysis start switch of the control unit 17 is pressed, the motor 19 is activated and the plunger 11 is rotated, and the notch groove 1 of the plunger 11 is rotated.
2 is in a position facing the first suction part 4a, the motor
The plunger 19 is connected to the plunger 11.
rotation stops. In this state, the motor 16 is operated and the plunger 11 is raised a predetermined distance.
As a result, a predetermined amount of the sample in the first liquid tank 7a is transferred to the first liquid suction pipe 6a, the first suction part 4a, and the notch groove 12.
are drawn into the cylinder 1 in sequence. Next, the motor 19 operates to move the plunger 1.
1 is rotated 90 degrees around its axis so that the cutout groove 12 of the plunger 11 faces the second suction part 4b, and then the motor 16 is operated to raise the plunger 11 a predetermined distance. The second
A predetermined amount of the PH buffer in the liquid tank 7b is sucked into the cylinder 1 in the same manner as above, and the PH at the time of suction is
Due to the inflow kinetic energy of the buffer solution and the diffusion phenomenon, the sample in the cylinder 1 and the newly drawn PH buffer solution are naturally mixed and homogenized. Furthermore,
Similarly, a predetermined amount of the coloring reagent in the third liquid tank 7c is accurately sucked into the cylinder 1 and mixed, whereby a coloring reaction is started within the cylinder 1.
If necessary, after leaving it until the reaction progresses and the transmittance becomes stable, the motor 19 is activated to move the plunger 1.
1 is rotated 90 degrees, and the notch groove 12 becomes the discharge part 5.
Then, in this state, the motor
The plunger 16 is activated and the plunger 11 is lowered. Thereby, the distance between the lower end of the plunger 11 and the bottom wall of the cylinder 1 is adjusted so as to be the optimum distance for transmittance measurement. After that, the light emitting section 13
The emitted light passes through the colored sample solution in the cylinder 11 and reaches the light receiving section 2. Here, it is converted into an electrical signal in proportion to the amount of transmitted light, and this electrical signal is sent from the signal lead line 3 to a recorder (not shown) and recorded. After the amount of transmitted light has been measured in this way, the motor 16 is operated to lower the plunger 11, and the colored sample solution in the cylinder 1 is thereby transferred to the notch 12, the discharge part 5, and the discharge pipe 9. and is discharged into the receiver 10. Next, the motor 19 is operated to rotate the plunger 11 by 90 degrees, so that the cutout groove 12 is opposed to the first suction portion 4a. As a result, the reaction cell device returns to its initial state, and the same operations are repeated thereafter, but all of the above operations are automatically performed by pulse signals sent from the control unit 17. .

In this embodiment, since the cylinder and plunger having the first to third suction parts are used to accurately mix the first to third liquids in predetermined amounts, multiple suction parts are mixed, unlike the conventional method. Compared to the case where mixing and dispensing are performed using a pump, the number of constituent units is small, and therefore the stability of operation accuracy and maintainability are good, and the manufacturing cost of the device is also relatively low. In addition, since the plunger moves up and down using a pulse motor, it is easy to control the moving distance of the plunger, and the suction amount of each liquid is extremely accurate, resulting in high analysis accuracy. It is something. Furthermore, since a light receiving part and a light emitting part are formed on the bottom wall of the cylinder and the lower end surface of the plunger,
Inhaling the sample, mixing the reagent, measuring, and discharging can all be performed with this device. Furthermore, when measuring transmitted light, the optical path length can be arbitrarily varied by appropriately adjusting the position of the plunger, making it possible to perform measurements under optimal conditions. It is something that can be measured.

In this embodiment, the intake sections 4a-4c and the discharge section 5 are formed on the cylinder 1 at 90° intervals in the circumferential direction of the cylinder 1, but this is not limited to this and they may be formed in any number and at any angle, and the light emitting section 13 and the light receiving section 2 are not limited to a tungsten lamp and a photodiode. Furthermore, the driving means for the plunger 11 is not limited to the above method, and heating and cooling means may be provided on the cylinder 1 to promote and adjust the reaction of the sample, reagent, etc. drawn into the cylinder 1, and various other modifications may be made without departing from the scope of the present invention.

The present invention includes a bottomed cylindrical cylinder in which a discharge part and a plurality of liquid suction parts are formed on the side wall, and a lower part of the cylinder is inserted into the cylinder in a liquid-tight manner so that it can rotate and move forward and backward. a plunger having a predetermined length along the axial direction from the lower end and having a notched groove that can communicate with the discharge part and the liquid suction part of the cylinder; and a drive mechanism that rotates the plunger to a predetermined position and advances and retreats the plunger. , a light receiving part and a light emitting part are provided opposite to each other on the bottom wall of the cylinder and the bottom end of the plunger, and the cylinder bottom wall and the bottom end of the plunger are measured by measuring the light emitted from the light emitting part with the light receiving part. By equipping the cylinder with a transmittance measuring mechanism that measures the transmittance of the liquid present between the cylinders, suction of the liquid sample, reaction, and transmittance measurement can be performed within the same cylinder, which reduces the number of constituent units and reduces operational efficiency. In addition to improving accuracy stability and maintainability, factors that reduce operational accuracy can be minimized, and analysis accuracy is significantly improved.

[Brief explanation of drawings]

FIG. 1 is a partially sectional side view showing an embodiment of the present invention, and FIG. 2 is an enlarged sectional plan view taken along the - line of the same embodiment. DESCRIPTION OF SYMBOLS 1...Cylinder, 2...Light receiving part, 3...Signal leader line, 4a-4c...1st to 3rd suction part, 5...
Discharge part, 6a to 6c...first to third liquid suction pipes,
7a to 7c...first to third liquid tanks, 8a to 8c...
...First to third liquids, 9...Discharge pipe, 10...Receiver, 11...Plunger, 12...Notch groove, 13
...Light emitting section, 14 ... Lead wire, 15 ... Round rack, 16 ... Pulse motor, 17 ... Control section, 1
8...Pinion, 19...Pulse motor.

Claims (1)

[Scope of utility model registration request] A bottomed cylindrical cylinder in which a discharge part and a plurality of liquid suction parts are formed on the side wall, and a lower part of the cylinder is inserted into the cylinder in a liquid-tight manner so as to be freely rotatable and retractable, and a cylinder is formed on the outer circumferential surface along the axial direction from the lower end. a plunger having a predetermined length and a notched groove that can communicate with the discharge portion and liquid suction portion of the cylinder; a drive mechanism that rotates the plunger to a predetermined position and advances and retreats; a bottom wall portion of the cylinder; and a light receiving part and a light emitting part respectively provided opposite to each other at the lower end of the plunger, and by measuring the light emitted from the light emitting part with the light receiving part, the transmission of the liquid existing between the cylinder bottom wall and the lower end of the plunger is detected. 1. A reaction cell device comprising a transmittance measuring mechanism for measuring a transmittance.