JPS63286270A - Feed for domestic animal - Google Patents

Abstract

PURPOSE:To use obtd. hydrolase as an important protein source for raising a domestic animal by enzymatic-treating corpuscle in blood of killed animal. CONSTITUTION:The blood obtd. from the killed animal is precipitated and practioned by using a centrifugal separator to obtain the corpuscle. Water is added at 2-5 times of this corpuscle by volume and protein concn. in the corpuscle solution is adjusted. After that, alkali is added to adjust pH of the solution to 7-10. The protein hydrolase protease is added to this at 0.1-10% of corpuscle protein and after executing hydrolysis under condition of 40-70 deg.C the solution temp., the centrifugal separation is executed. Then, the supernatant liquid is refined by activated carbon treatment or using ion exchange resin. In the hydrolase obtd. by such a way, as a large part thereof possesses by globin protein and in the other, the minerals, such as calcium, phosphorous, are contained, the raising is promoted by adding in feed for domestic animal.

Description

[Detailed description of the invention]

(Industrial Application Field) The present invention provides #! Efficient growth of planks can be achieved by adding to livestock feed an enzymatic hydrolyzate obtained by hydrolyzing proteins using whole blood cell enzymes in the animal's blood, or a mixture of this and plasma. (Prior art) The use of layered blood for feed has traditionally been called blood meal, which has been absorbed into other substances or made into a powder using a drying method and used as an auxiliary raw material for raw feed. It has different characteristics such as digestibility, darkness, and odor. In addition, as another use of layered blood, the so-called plasma fraction obtained by blood iY centrifugation is widely used in the fields of foods, medicines, and other fields. However, the usefulness of the hemocyte fraction #3, which remains after separation, has not yet been established and is in the form of a lump. (The main point to be solved by the present invention) As mentioned above, in view of the usefulness of high protein contained in blood cells in the form of lumps in which the utilization of blood cells is not carried out, the present invention is based on the fact that high protein can be obtained by enzyme treatment. The hydrolyzate of blood cells produced by the blood cells is
In particular, we have discovered that this product can serve as an important protein source for animals during the lactation period, weaning period, and for fattening, and our purpose is to provide a method for utilizing the cumulative hydrolyzate. Furthermore, the present invention also solves various problems regarding the treatment of the 1111 liquid that is carried by beach users. As a practical use, we investigated the use of θ] in the original feed, and found that a white powder product was obtained as a hydrolyzate by allowing the conventional protein hydrolase protease to act on blood cells and then drying it. This white powder product is obtained by hydrolyzing hemoglobin, which is a conjugated protein in m bulbs, with grotease, which is a protein-rich hydrolyzed scale, to remove the heme content. ◎ To explain the present invention in more detail, the blood collected in the present invention can be collected from cows, calves, or commonly used in water bottles. The collected blood can be used without being limited to the f3II class.The collected blood is centrifuged to obtain a precipitate fraction, that is, blood cells.If necessary, by repeating centrifugation, blood cells can be obtained. The method for obtaining blood cells is to use a centrifugal fragmentation machine, which is simple and best, but the method is not limited to dirt. The law and the way it is fulfilled (NEW FOOD INDDSTERVo)
1, 26 No. 8 1984). In other words, add 2 to 5 times the volume of water to each blood cell and adjust the amount of protein in the blood cell bath. Add alkali to adjust the pH of the bath liquid.
Adjust to 7 to 10, preferably pH 8, 2 to 8.7,
Teru. This is added to 0.1-10% of the amount of lysate and cylindrical protein purchased.
In addition, after hydrolysis is carried out in a bath and at a temperature of 40 to 70°C, single-point separation is carried out, and the upper plate is treated with activated carbon or purified via the ion exchange side. The yield Y of the enzymatic hydrolyzate can also be determined by adding water to the residue after centrifugation and repeating centrifugation. Enzyme hydrolysis after modification? Although it is possible to use I-Kun-Kouyakusha as it is or in the form of a vinegar liquid by mixing it with plasma, it is recommended to freeze-dry it from the viewpoint of changes over time, storage, and transportation of the obtained eggplant. It is more preferable to use the powder by drying or spray drying the powder. The enzymatic hydrolyzate obtained in this way is added to the original feed, but it is preferable to add it to artificial milk during the lactating period, milk replacer during the weaning period, and mixed feed during the early fattening period. However, it is appropriate to add 0.1 to 10% in terms of solid content to the copper material for water jars as an enzymatic hydrolyzate or by mixing it with plasma, or add more than dirt. However, there is no intermission. The protein composition of the white powder product obtained by enzymatic hydrolysis of blood cells is a globin protein in which e-type amino acids are peptide-bonded. The molecular weight of globin protein added by liquid chromatography is concentrated in the range of 1000 to 2000,
Broadly distributed between 500 and 5000. In addition, this white powdered product has almost no off-odor of natural animal odor.
It has a relatively low molecular weight and is high in minerals such as calcium and minerals. When I added the sea bream ingredients from the period when T'a''Jb San was added to the diet, the effect of increasing Mizuho's body by 1 and improving the efficiency of medical treatment was delayed.The following describes the present invention. However, the present invention is not limited to such an example and is not limited to the above. , plasma and blood cells (separate into two. Add three times the amount of water to the obtained km cells, and use five times Nap) ['j\ to PR case 8.
Tune to 5. To this, protease was added at 3% based on the number of blood cells in the bath, and the mixture was kept at a temperature of 55°C for 3 hours. During this time PH
continues to maintain a constant value. Next, after inactivating the enzyme using Kogyu MCI, the pH was adjusted to 5.01 m using 5-meter N30H, centrifugation was performed, and activated charcoal was added to the Joisei liquid, followed by 1 sip.
By drying, 1.1 kg of white powdery dried material was obtained. The amount of protein in this dried product can be determined by the Schönkeldahl method.
The total content was about 85%. The vinegar cumulative hydrolyzate obtained by repeating the above procedures was added to commercially available feed at a ratio of 1%, 3%, and 5%, and the mixture was added to the test plots at Ichi Jl 12 Tsuru. The feed was added to the control group, and the lactating piglets, which were around 6 months old, were given 21
The increase in pay and feed conversion rate of suckling piglets fed g1 were compared. The weight distribution of suckling piglets in each area is on average.

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I tried each 79A. The results are shown in Table 1. The feed conversion ratio in Table 1 represents the percentage of feed intake per paw weight gain, and is expressed as a linear equation. As is clear from Table 1 of Table 1, when the obtained enzymatic hydrolyzate is added to commercially available feed, it is extremely effective in improving weight gain and feed efficiency. Example 2゜The enzymatic hydrolyzate obtained in the same manner as in Example 1 was mixed with dried plasma V at a ratio of 1:1, and this was added to commercial feed at ratios of 1%, 3%, and 5%. Each of the following was added to the test plots, and fed to suckling piglets around body M6 for two weeks based on the method of Example 1, and the pay increase and feed conversion rate of the suckling piglets were compared. The lactating piglets should be kept at 6 to 7 lbs. so that the weight distribution is average.
A head-to-head friend. The results are shown in Table 2. As is clear from the second coating, when the mixture of enzyme hydrolyzate and dried plasma was used by adding it to the commercial steel feed, it was clearly effective in improving constant loss and increasing feed efficiency. .

Claims (2)

[Claims] (1) A livestock feed characterized by adding an enzyme hydrolyzate obtained by enzymatically treating blood cells in slaughtered blood. (2) A feed for livestock, characterized in that an enzyme hydrolyzate obtained by enzymatically treating blood cells in slaughtered blood is mixed with plasma and added to the mixture.