JPS63286158A - Disinfection of contact lens - Google Patents
Disinfection of contact lensInfo
- Publication number
- JPS63286158A JPS63286158A JP62122133A JP12213387A JPS63286158A JP S63286158 A JPS63286158 A JP S63286158A JP 62122133 A JP62122133 A JP 62122133A JP 12213387 A JP12213387 A JP 12213387A JP S63286158 A JPS63286158 A JP S63286158A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- enzyme
- lens
- immobilized
- contact lens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 24
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 219
- 238000000034 method Methods 0.000 claims abstract description 45
- 108010093096 Immobilized Enzymes Proteins 0.000 claims abstract description 42
- 230000000249 desinfective effect Effects 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 46
- 108090000790 Enzymes Proteins 0.000 abstract description 46
- 102000016938 Catalase Human genes 0.000 abstract description 5
- 108010053835 Catalase Proteins 0.000 abstract description 5
- 229910052751 metal Inorganic materials 0.000 abstract description 5
- 239000002184 metal Substances 0.000 abstract description 5
- 102000003992 Peroxidases Human genes 0.000 abstract description 4
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract description 4
- -1 pulp Substances 0.000 abstract description 4
- 239000000919 ceramic Substances 0.000 abstract description 2
- 238000004132 cross linking Methods 0.000 abstract description 2
- 230000003100 immobilizing effect Effects 0.000 abstract description 2
- 229920003023 plastic Polymers 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 238000000354 decomposition reaction Methods 0.000 description 18
- 239000007864 aqueous solution Substances 0.000 description 12
- 239000003054 catalyst Substances 0.000 description 11
- 239000000645 desinfectant Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000008213 purified water Substances 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 206010020751 Hypersensitivity Diseases 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- FZERHIULMFGESH-UHFFFAOYSA-N N-phenylacetamide Chemical compound CC(=O)NC1=CC=CC=C1 FZERHIULMFGESH-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- RCJVRSBWZCNNQT-UHFFFAOYSA-N dichloridooxygen Chemical compound ClOCl RCJVRSBWZCNNQT-UHFFFAOYSA-N 0.000 description 2
- 210000004400 mucous membrane Anatomy 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229940107700 pyruvic acid Drugs 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- MTPJEFOSTIKRSS-UHFFFAOYSA-N 3-(dimethylamino)propanenitrile Chemical compound CN(C)CCC#N MTPJEFOSTIKRSS-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical class C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-VVKOMZTBSA-N Dideuterium Chemical compound [2H][2H] UFHFLCQGNIYNRP-VVKOMZTBSA-N 0.000 description 1
- 244000043261 Hevea brasiliensis Species 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 229960001413 acetanilide Drugs 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- TVQLLNFANZSCGY-UHFFFAOYSA-N disodium;dioxido(oxo)tin Chemical compound [Na+].[Na+].[O-][Sn]([O-])=O TVQLLNFANZSCGY-UHFFFAOYSA-N 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002731 mercury compounds Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 229920003052 natural elastomer Polymers 0.000 description 1
- 229920001194 natural rubber Polymers 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 229940079864 sodium stannate Drugs 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 239000012929 tonicity agent Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、コンタクトレンズの消毒方法に関する。さら
に詳しくは、本発明はコンタクトレンズを過酸化水素に
より消毒する方法において、該過酸化水素を分解する手
段に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for disinfecting contact lenses. More specifically, the present invention relates to a method for disinfecting contact lenses with hydrogen peroxide, and to means for decomposing hydrogen peroxide.
［従来の技術およびその問題点コ
コンタクトレンズ、とくに含水性のコンタクトレンズは
、使用しているうちに（レンズ保存中などに）レンズ表
面やレンズ内に細菌などが繁殖して汚染されやすい。そ
のために、定期的にコンタクトレンズを消毒する必要が
ある。[Prior art and its problems]Contact lenses, especially water-containing contact lenses, are susceptible to contamination due to the growth of bacteria on the lens surface and inside the lens during use (such as during lens storage). For this reason, it is necessary to disinfect contact lenses regularly.
コンタクトレンズを消毒する方法には、熱を加えて消毒
する加熱消毒方法と殺菌剤により消毒するコールド消毒
方法（化学的消毒方法）とがある。There are two methods for disinfecting contact lenses: a heat disinfection method that disinfects by applying heat, and a cold disinfection method (chemical disinfection method) that disinfects with a disinfectant.
さらに、コールド消毒方法は、塩化ベンザルコニウム、
クロルヘキシジン塩、およびチメロサールのような有機
水銀化合物などの通常の殺菌剤を用いる方法と、容易に
分解して無毒化できる過酸化水素を用いる方法とに分け
やれる。In addition, cold disinfection methods include benzalkonium chloride,
Methods can be divided into methods using conventional disinfectants such as chlorhexidine salts and organic mercury compounds such as thimerosal, and methods using hydrogen peroxide, which can be easily decomposed and rendered non-toxic.
そして、過酸化水素を用いる消毒方法において、その過
酸化水素を分解する手段は、白金などの金属触媒を用い
るもの、ピルビン酸などの還元剤を用いるもの、カタラ
ーゼ、ペルオキシダーゼなどの酵素触媒を用いるものに
分けられる。In disinfection methods that use hydrogen peroxide, the means for decomposing hydrogen peroxide are those that use metal catalysts such as platinum, those that use reducing agents such as pyruvic acid, and those that use enzyme catalysts such as catalase and peroxidase. It can be divided into
以下、加熱消毒方法およびコールド消毒方法が有する問
題点について述べる。Problems with the heat sterilization method and the cold sterilization method will be described below.
（１）加熱消毒方法
加熱する手段としては、一般に電気による加熱手段が採
用されている。そして、電気による加熱手段を作動させ
るためには通電の際に電源を必要とする。この電源とし
ては通常乾電池は用いられずに、電力会社などから供給
される一般家庭用の１００■電源が用いられている。こ
のため旅行などの際には、適当な電源がない所もしくは
電源が異なる（たとえば、電流、電圧、周波数またはコ
ンセントの形などが異なる）所では加熱手段に通電する
ことができないという問題がある。(1) Heating sterilization method Electrical heating means are generally used as heating means. In order to operate the electric heating means, a power source is required when energizing. Normally, a dry cell battery is not used as this power source, but a general household 100cm power source supplied from an electric power company or the like is used. Therefore, when traveling, etc., there is a problem that the heating means cannot be energized in places where there is no suitable power source or where the power source is different (for example, the current, voltage, frequency, or type of outlet is different).
また、加熱消毒をするための装置がややもするとコンパ
クトな装置とはいえず、持ち運ぶにはかさばるという不
便もある。In addition, there is also the inconvenience that the device for heat sterilization is not a compact device and is bulky to carry.
さらに、コンタクトレンズを完全に消毒するためには、
塩水溶液中でおよそ１時間も加熱処理（加熱開始から煮
沸（厚生省基準：１００°Ｃｌ２Ｏ分以」二）、および
放冷して処理を終了するまで）をする必要があり、その
加熱処理時間の長さには煩わしさを感じさせることか多
々ある。In addition, to completely disinfect contact lenses,
It is necessary to perform heat treatment for approximately one hour in a salt aqueous solution (from the start of heating to boiling (Ministry of Health and Welfare standards: 100°C Cl2O minutes or more), and to cooling to end the treatment). There are many things that make me feel bothered by the length.
（２）コールド消毒方法
（ａ）過酸化水素以外の通常の殺菌剤を用いる方法殺菌
剤を含む水溶液中にコンタクトレンズを浸漬してレンズ
を消毒する。しかしながら、とくに含水性のコンタクト
レンズにあってはそのレンズ中に殺菌剤が侵入し蓄積、
すなわち収着されやすく、レンズを塩水溶液で充分すす
いでも、容易にそのレンズ中の殺菌剤を取り除くことは
できないばあいがある。そして、このようなばあいのコ
ンタクトレンズを眼に装用すると、レンズから徐々に放
出される殺菌剤により、眼の粘膜などに刺激を与えたり
、アレルギー反応を起こす原因にもなりやすいという問
題がある。(2) Cold disinfection method (a) Method using a conventional disinfectant other than hydrogen peroxide Contact lenses are sterilized by immersing them in an aqueous solution containing a disinfectant. However, especially with water-containing contact lenses, disinfectants can enter and accumulate in the lenses.
In other words, the disinfectant in the lens is easily sorbed, and even if the lens is thoroughly rinsed with an aqueous salt solution, the disinfectant in the lens may not be easily removed. When such contact lenses are worn on the eyes, there is a problem in that the bactericidal agent gradually released from the lenses tends to irritate the mucous membranes of the eyes and cause allergic reactions.
重過酸化水素を用いる方法
過酸化水素は容易に分解して無毒化することかできるの
で、コンタクトレンズのレンズ表面やレンズ中に存在す
る過酸化水素を完全に分解してしまえば、前記眼の粘膜
などに刺激を与えたり、アレルギー反応を起こしたりす
る問題は回避できるという利点がある。また、従来の過
酸化水素を分解する各方法には以下のような相違点があ
る。Method using heavy hydrogen peroxide Hydrogen peroxide can be easily decomposed and rendered non-toxic, so if the hydrogen peroxide present on the lens surface or in the contact lens is completely decomposed, it will not cause damage to the eye. It has the advantage of avoiding problems such as irritation to mucous membranes and allergic reactions. Furthermore, the conventional methods for decomposing hydrogen peroxide have the following differences.
（イ）白金などの金属触媒による分解
過酸化水素に対する分解力は大きいが、酵素触媒はどそ
の分解は迅速ではない。(a) Decomposition by metal catalysts such as platinumAlthough the decomposition power of hydrogen peroxide is high, enzyme catalysts do not decompose it quickly.
（ロ）ピルビン酸などの還元剤による分解過酸化水素に
対する分解力は金属触媒や酵素触媒と比べて小さい。(b) Decomposition power of hydrogen peroxide using reducing agents such as pyruvic acid is smaller than that of metal catalysts or enzyme catalysts.
（ｌ＼）カタラーゼ、ペルオキシダーゼなどの酵素触媒
による分解
過酸化水素に対する分解力は大きく、かつその分解は迅
速である。(l\) Decomposition by enzyme catalysts such as catalase and peroxidase The decomposition power for hydrogen peroxide is large and the decomposition is rapid.
前記従来技術が有する相違点かられかるように、コンタ
クトレンズを消毒するには分解して無毒化することがで
きる過酸化水素を用いるのかもっとも好ましく、そして
その過酸化水素を分解するには分解力が大きくかつ分解
が迅速である酵素触媒を用いるのがもつとも好ましい。As can be seen from the differences in the prior art, it is most preferable to use hydrogen peroxide, which can be decomposed and rendered non-toxic, to disinfect contact lenses, and decomposition power is required to decompose hydrogen peroxide. It is also preferable to use an enzyme catalyst that has a large amount of oxidation and decomposes quickly.
従来の過酸化水素を酵素触媒により分解する方法（特開
昭８０−２１７３３３号、特開昭６１−７７８２２号、
特開昭８２−６３９１１ｉ号各公報参照）は、酵素を水
溶液に溶解もしくは最終的に水溶液中に分散させて、過
酸化水素と酵素とを接触させて分解作用を生じさせてい
る。しかしながら、従来のこうしたシステムでは、水中
に浮遊している酵素がレンズに直接接触するために酵素
タンパク質がレンズに付着し、洗浄に手間がかかる。さ
らに放置しておくとレンズが白濁する原因になったり、
そのレンズを眼に入れると、付着したタンパク質により
アレルギー反応を起こさせる原因になりやすいという問
題点がある。Conventional methods for decomposing hydrogen peroxide using enzyme catalysts (JP-A-80-217333, JP-A-61-77822,
JP-A No. 82-63911i) discloses that an enzyme is dissolved in an aqueous solution or finally dispersed in an aqueous solution, and hydrogen peroxide and the enzyme are brought into contact to cause a decomposition effect. However, in such conventional systems, the enzyme suspended in water comes into direct contact with the lens, which causes the enzyme protein to adhere to the lens, making cleaning time-consuming. If left untreated, the lens may become cloudy,
When the lens is put into the eye, the attached proteins tend to cause an allergic reaction.
［発明が解決しようとする問題点］
コンタクトレンズを過酸化水素により消毒し、消毒処理
中もしくは消毒処理したのちの処理溶液中に存在してい
る過酸化水素もしくはレンズ表面やレンズ内部に付着し
ている過酸化水素を酵素触媒により速やかに分解する方
法において、酵素タンパク質がレンズに付着しないよう
にすることを目的とする。[Problems to be solved by the invention] Contact lenses are disinfected with hydrogen peroxide, and hydrogen peroxide present in the treatment solution during or after the disinfection process or attached to the lens surface or inside the lens is removed. The purpose of this method is to prevent enzyme proteins from adhering to lenses in a method for rapidly decomposing hydrogen peroxide present in the lens using an enzyme catalyst.
［問題点を解決するための手段］
すなわち本発明は、コンタクトレンズを過酸化水素によ
り消毒する方法において、消毒処理中もしくは消毒処理
後に過酸化水素を分解する手段として固定化酵素を用い
ることを特徴とするコンタクトレンズの消毒方法に関す
る。[Means for Solving the Problems] That is, the present invention is characterized in that, in a method for disinfecting contact lenses with hydrogen peroxide, an immobilized enzyme is used as a means for decomposing hydrogen peroxide during or after the disinfection process. This article relates to a method for disinfecting contact lenses.
［作 用］
過酸化水素によりコンタクトレンズを消毒する。こうし
た消毒処理をしたコンタクトレンズを無毒化せずに眼に
装用すると、眼に重篤な障害を引き起こすので、酵素触
媒により速やかに過酸化水素を分解する。ただし、酵素
は固定されているので、酵素タンパク質が処理溶液中に
遊離し、コンタクトレンズ表面などに付着することはな
い。[Action] Disinfects contact lenses with hydrogen peroxide. If such disinfected contact lenses are worn in the eyes without being detoxified, it will cause serious damage to the eyes, so the hydrogen peroxide is quickly decomposed using an enzyme catalyst. However, since the enzyme is fixed, the enzyme protein will not be released into the treatment solution and will not adhere to the surface of the contact lens or the like.
［実施例］
消毒処理に用いる過酸化水素には、通常水溶液になった
ものを使用する。過酸化水素溶液中の過酸化水素の濃度
は、消毒効果を有する濃度であり、かつコンタクトレン
ズに形状変化などの損傷を与えない濃度であればよく、
およそ０．１〜１０ｗ１ｖ％の範囲内にあるのが好まし
い。[Example] Hydrogen peroxide used for disinfection treatment is usually in the form of an aqueous solution. The concentration of hydrogen peroxide in the hydrogen peroxide solution may be a concentration that has a disinfecting effect and does not cause damage to the contact lens such as changing its shape.
It is preferably within the range of approximately 0.1 to 10w1v%.
ＬＯＷ／Ｖ％よりも過酸化水素の濃度が高いと、レンズ
に損傷を与える危険があり、０．ｌＷ／Ｖ％よりも過酸
化水素の濃度が低いと、充分な消毒効果かえられなくな
ってしまうおそれがある。より好ましい過酸化水素の濃
度は、０．５〜５ Ｗ／Ｖ％であり、市販されている過
酸化水素水でもっとも一般的で入手しやすく、レンズに
損傷を与えずに充分な消毒効果を有するという意味にお
いては、３ｗ１ｖ％程度の濃度のものがもっとも好まし
い。If the concentration of hydrogen peroxide is higher than LOW/V%, there is a risk of damaging the lens; If the concentration of hydrogen peroxide is lower than 1W/V%, there is a risk that a sufficient disinfecting effect may not be obtained. A more preferable concentration of hydrogen peroxide is 0.5 to 5 W/V%, which is the most common and easily available hydrogen peroxide solution on the market, and which provides sufficient disinfecting effect without damaging the lens. In the sense that it has a concentration of about 3w1v%, it is most preferable.
また、過酸化水素溶液のほかに過酸化尿素などの過酸化
化合物を水に溶解して調製した水溶液を用いてもよい。In addition to the hydrogen peroxide solution, an aqueous solution prepared by dissolving a peroxide compound such as urea peroxide in water may also be used.
コンタクトレンズの消毒に用いる過酸化水素溶液は、通
常市販されている過酸化水素など（たとえば、３ Ｗ／
Ｖ％の濃度の過酸化水素水）に適宜、安定化剤、緩衝剤
、等張化剤などを加えて調製する。The hydrogen peroxide solution used to disinfect contact lenses is usually commercially available hydrogen peroxide (for example, 3 W/
It is prepared by adding a stabilizer, a buffer, an isotonizing agent, etc. as appropriate to hydrogen peroxide solution (with a concentration of V%).
安定化剤は、過酸化水素がコンタクトレンズの消毒を開
始する前に容易に分解してしまわないようにするために
用いられる。たとえば、リン酸、バルビッール酸、尿酸
、アセトアニリド、オキシキノリン、ピロリン酸ナトリ
ウム、ツェナセチン、すず酸ナトリウムなどがあげられ
る。Stabilizers are used to keep the hydrogen peroxide from degrading easily before disinfecting the contact lens. Examples include phosphoric acid, barbituric acid, uric acid, acetanilide, oxyquinoline, sodium pyrophosphate, zenacetin, and sodium stannate.
緩衝剤は、処理溶液のｐＨを安定化させるために用いら
れる。たとえば、リン酸、ホウ酸などがあげられる。Buffers are used to stabilize the pH of processing solutions. Examples include phosphoric acid and boric acid.
等張化剤は、処理溶液の浸透圧をコンタクトレンズにと
ってもっとも好ましい状態の浸透圧（涙液とほぼ同じ浸
透圧）と同等にするために用いられる。たとえば、塩化
ナトリウム、塩化カリウムなどがあげられる。The tonicity agent is used to equalize the osmotic pressure of the treatment solution to the most favorable osmotic pressure for contact lenses (approximately the same osmotic pressure as tear fluid). Examples include sodium chloride and potassium chloride.
過酸化水素を分解する酵素としては、カタラーゼとペル
オキシダーゼの２種類があげられる。There are two types of enzymes that decompose hydrogen peroxide: catalase and peroxidase.
酵素の含有量（単位数）はとくに限定されるものではな
いが過酸化水素を効率的に分解するのに充分な量の酵素
が固定化する担体中に存在するのが望ましい。Although the content (number of units) of the enzyme is not particularly limited, it is desirable that a sufficient amount of enzyme be present in the immobilized carrier to efficiently decompose hydrogen peroxide.
酵素の含有量と過酸化水素の分解時間との間には相関関
係があり、また使用する酵素の種類、固定化する担体の
種類または固定化酵素の状態（たとえば、多孔質状態の
ように固定化酵素の表面積が大きいか否かなど）により
必要とされる酵素の含有量は異なるので、固定化酵素の
具体的な好ましい酵素含有量というのは一概には言えな
い。すなわち、所望の消毒に必要な過酸化水素の有効濃
度維持時間または分解時間などに対して、適宜酵素の含
有量を調製した固定化酵素を使用することになる。たと
えば、短時間（たとえば３０分以内）または就寝中（約
７時間程度）の間に分解できるように設定するばあい、
そのように固定化酵素の酵素含有量を多くしたり少なく
したりして調節したものを使用してもよい。There is a correlation between the enzyme content and the decomposition time of hydrogen peroxide. Since the required enzyme content differs depending on whether the surface area of the immobilized enzyme is large or not, it is difficult to say with certainty the specific preferred enzyme content of the immobilized enzyme. That is, an immobilized enzyme is used whose content is adjusted appropriately for the effective concentration maintenance time or decomposition time of hydrogen peroxide required for desired disinfection. For example, if you set it so that it can be decomposed for a short period of time (for example, within 30 minutes) or while you are sleeping (about 7 hours),
The enzyme content of the immobilized enzyme may be adjusted by increasing or decreasing it in this way and may be used.
酵素の固定化方法としては、共有結合、イオン結合、物
理的吸着などによる担体結合法、架橋法、包括法などの
従来技術を用いうる。As a method for immobilizing the enzyme, conventional techniques such as a carrier binding method using covalent bonding, ionic bonding, physical adsorption, etc., crosslinking method, entrapment method, etc. can be used.
固定化に用いる担体としては、プラスチック、ラバーな
どの合成樹脂、紙、パルプ、フェルト、天然ゴムなどの
天然の素材を原料とするもの、セラミックまたは金属な
どの通常固定化に用いられているものであればよく、も
しくはこれらを組合わせて用いてもよい。The carriers used for immobilization include those made from synthetic resins such as plastic and rubber, natural materials such as paper, pulp, felt, and natural rubber, and those normally used for immobilization such as ceramics and metals. Any one of them may be used, or a combination of these may be used.
そのほか、過酸化水素の分解に固定化酵素を使用してい
るうちに該酵素の酵素活性が低下したばあい、または酵
素活性の低い固定化酵素を使用しているばあい、固定化
酵素を新しいものと取り替えたり、または新しいものを
さらに追加したりしてもよい。In addition, if the enzyme activity of the immobilized enzyme decreases while using it to decompose hydrogen peroxide, or if an immobilized enzyme with low enzyme activity is used, replace the immobilized enzyme with a new one. You can replace it with something else, or add something new.
固定化酵素の状態や形状としては、ゲル状、多孔質状、
ボタン形状、ビーズ形状などいかなる状態や形状であっ
てもかまわない。ただし、多孔質状やビーズ形状などの
ようにすれば、それだけ固定化酵素の有効表面積が大き
くなるので、過酸化水素の分解もそれだけ効率的に行う
ことができる。すなわち、固定化酵素の表面積か大きけ
れば、表面積の小さいものより酵素含有量が少なくても
、同等の過酸化水素の分解能力を発揮させることができ
る。The state and shape of immobilized enzymes include gel-like, porous,
It may be in any state or shape, such as a button shape or a bead shape. However, if the shape is porous or bead-shaped, the effective surface area of the immobilized enzyme becomes larger, and hydrogen peroxide can be decomposed more efficiently. That is, if the surface area of the immobilized enzyme is large, even if the enzyme content is lower than that of an immobilized enzyme with a small surface area, it can exhibit the same ability to decompose hydrogen peroxide.
本発明のコンタクトレンズの消毒方法は、コンタクトレ
ンズの消毒の工程と過酸化水素の分解の工程からなる消
毒システムである。消毒システムとしては、たとえば単
一溶液によるコンタクトレンズの消毒と過酸化水素の分
解からなる１液システム、２種の溶液によるコンタクト
レンズの消毒と過酸化水素の分解からなる２液システム
、そのほかにコンタクトレンズを過酸化水素により消毒
処理したのち、固定化酵素に直接接触させて過酸化水素
を分解する直接接触システムなどがあげられる。The contact lens disinfection method of the present invention is a disinfection system comprising a contact lens disinfection step and a hydrogen peroxide decomposition step. Disinfection systems include, for example, a one-liquid system that disinfects contact lenses with a single solution and decomposes hydrogen peroxide, a two-liquid system that disinfects contact lenses with two solutions and decomposes hydrogen peroxide, and a two-liquid system that disinfects contact lenses with two solutions and decomposes hydrogen peroxide. Examples include a direct contact system in which the lens is disinfected with hydrogen peroxide and then brought into direct contact with an immobilized enzyme to decompose the hydrogen peroxide.
前記１液システムとはコンタクトレンズを過酸化水素溶
液により消毒すると同時にまたは一定時間消毒したのち
に、該溶液に固定化した酵素を接触させて過酸化水素を
分解する方法をいう。The one-liquid system refers to a method in which a contact lens is disinfected with a hydrogen peroxide solution and at the same time or after disinfection for a certain period of time, an immobilized enzyme is brought into contact with the solution to decompose hydrogen peroxide.
該１液システムにおいて過酸化水素と固定化＝ １
１ −
酵素とを接触させる方法としては、たとえば以下の、
■あらかじめ消毒に用いる容器の中に固定化酵素を入れ
ておき、容器の中に過酸化水素水溶液を入れ、そののち
すぐにコンタクトレンズを入 ・れて消毒する方法、
■先に消毒容器の中に過酸化水素水溶液を入れておき、
その中にコンタクトレンズを入れて消毒し、そののちた
だちにまたは一定時間経過後に固定化酵素を容器の中に
入れる方法、および■消毒容器内に固定化酵素と過酸化
水素とが接触しない位置にあらかじめセットしておき、
何らかの方法、たとえば、容器の転倒などの方法により
過酸化水素を含む水溶液と固定化酵素とか接触する方法
などがあげられる。In the one-component system, hydrogen peroxide and immobilization = 1
1 - For example, the method of contacting the enzyme is as follows: ■ Place the immobilized enzyme in a container used for disinfection in advance, pour a hydrogen peroxide solution into the container, and then immediately put the contact lens on. How to sterilize by putting it in a container, ■First, put a hydrogen peroxide solution in a sterilization container.
A method in which a contact lens is placed in the container to be disinfected, and then the immobilized enzyme is placed in the container immediately or after a certain period of time has elapsed; Set it and
For example, a method may be used in which the immobilized enzyme is brought into contact with an aqueous solution containing hydrogen peroxide by inverting a container or the like.
前記２液システムとはコンタクトレンズを過酸化水素溶
液により消毒したのち、該溶液がらレンズを取り出し、
固定化した酵素の入った水溶液中にコンタクトレンズを
移して浸漬し、レンズ表面やレンズ内部に残留していた
過酸化水素を固定化酵素の入った水溶液中に拡散させて
分解する方法をいう。The two-liquid system is a method in which contact lenses are disinfected with a hydrogen peroxide solution, the lenses are removed from the solution, and
A method in which a contact lens is transferred and immersed in an aqueous solution containing an immobilized enzyme, and hydrogen peroxide remaining on the lens surface or inside the lens is diffused into the aqueous solution containing the immobilized enzyme and decomposed.
前記直接接触システムとは、たとえばシート状に固定化
した酵素を形成しておき、コンタクトレンズを過酸化水
素溶液により消毒したのち、該溶液からレンズを取り出
し、前記シートにレンズを包み込み、レンズ表面やレン
ズ内部に残留していた過酸化水素を固定化した酵素に直
接接触させて分解する方法をいう。The above-mentioned direct contact system is a system in which, for example, an immobilized enzyme is formed in the form of a sheet, a contact lens is disinfected with a hydrogen peroxide solution, the lens is taken out from the solution, the lens is wrapped in the sheet, and the lens surface and A method of decomposing hydrogen peroxide remaining inside the lens by bringing it into direct contact with an immobilized enzyme.
以下に、本発明を実施例を用いて説明するが、もとより
本発明はかかる実施例のみに限定されるものではない。EXAMPLES The present invention will be explained below using Examples, but the present invention is not limited to these Examples.
参考例１ （固定化酵素の作製）
アクリルアミド７．５ｇ、 Ｎ、Ｎ’−メチレンビスア
クリルアミド０゜４ｇ１カタラーゼ１５０，０００単位
を０．１モルＴｒｉｓ−ＨＣ１緩衝液（ＰＨ７） ４
ｍｌに溶解し、５ Ｗ／Ｖ％β−ジメチルアミノプロピ
オニトリル０．５ｍｌを加えた。Reference Example 1 (Preparation of immobilized enzyme) 7.5 g of acrylamide, 0.4 g of N,N'-methylenebisacrylamide, 150,000 units of catalase, and 0.1 mol Tris-HC1 buffer (PH7) 4
ml, and 0.5 ml of 5 W/V% β-dimethylaminopropionitrile was added.
ついで１０ｍ１シリンジに全量を吸い取り、そののち２
５°Ｃの恒温水槽に入れ、急激な重合反応による発熱を
抑えながら、円柱状（寸法：直径１５ｆｆｌｆｆ１１高
さ１５Ｉｎｍ）に成形した。Next, absorb the entire amount into a 10ml syringe, and then
It was placed in a constant temperature water bath at 5°C and molded into a cylindrical shape (dimensions: diameter 15ffff11 height 15 Inm) while suppressing heat generation due to rapid polymerization reaction.
えられた酵素を含むポリアクリルアミドゲルを０．１モ
ルＴｒｉｓ−ＨＣ＃緩衝液（ｐＨ７）で充分に洗浄して
固定化酵素をえた。The polyacrylamide gel containing the obtained enzyme was thoroughly washed with 0.1M Tris-HC# buffer (pH 7) to obtain the immobilized enzyme.
実施例１ （１液システム）
０．９シバ％濃度の塩化ナトリウム水溶液に過酸化水素
水を過酸化水素濃度が３ Ｗ／Ｖ％となるように加えて
０，０１モルのリン酸緩衝液により約ｐＨ７に緩衝され
たコンタクトレンズを消毒するための過酸化水素溶液を
調製した。Example 1 (1-liquid system) Hydrogen peroxide solution was added to a sodium chloride aqueous solution with a concentration of 0.9% so that the hydrogen peroxide concentration was 3 W/V%, and the mixture was mixed with a 0.01M phosphate buffer. A hydrogen peroxide solution for disinfecting contact lenses was prepared that was buffered to approximately pH 7.
約３０ｍ１容の小瓶を１２個用意し、それぞれの小瓶に
前記過酸化水素溶液を１［１ｍｌずつ入れた。各小瓶の
処理溶液中にソフトコンタクトレンズ（■メニコン製、
商品名メニコンソフトＭ）をそれぞれ１枚ずつ入れ、２
個の小瓶で１セツトとし、２５℃の恒温水槽内にて３０
分間放置し、レンズ内に過酸化水素を完全に浸透させた
。Twelve small bottles each having a volume of about 30 ml were prepared, and 1 [1 ml] of the above hydrogen peroxide solution was placed in each small bottle. Soft contact lenses (made by Menicon,
Put one piece of product name Menicon Soft M) in each, and
One set is made up of several small bottles and placed in a constant temperature water bath at 25℃ for 30 minutes.
The lens was left for a minute to allow the hydrogen peroxide to completely penetrate into the lens.
前記ソフトコンタクトレンズを入れた各処理溶液中に参
考例１にて作製した固定化酵素をそれぞれ入れ、過酸化
水素の分解反応を開始させた。The immobilized enzyme prepared in Reference Example 1 was added to each treatment solution containing the soft contact lens, and the decomposition reaction of hydrogen peroxide was started.
一定時間後、すなわち０分後、３分後、７分後、１５分
後、３０分後および６０分後に１セツト（２個）ずつ固
定化酵素を取り除くことにより、分解反応を停止させ、
各処理溶液中の過酸化水素濃度を日本薬局方にしたがい
、０．ＩＮ過マンガン酸カリウムにて定量し、１セツト
（２個）の平均値から各一定時間後の処理溶液中の過酸
化水素の濃度（Ｗ／Ｖ％）を求めた。After a certain period of time, that is, after 0 minutes, 3 minutes, 7 minutes, 15 minutes, 30 minutes, and 60 minutes, the decomposition reaction is stopped by removing one set (two pieces) of the immobilized enzyme,
The hydrogen peroxide concentration in each treatment solution was determined according to the Japanese Pharmacopoeia. It was quantified using IN potassium permanganate, and the concentration (W/V%) of hydrogen peroxide in the treatment solution after each fixed time was determined from the average value of one set (two samples).
第１表および第１図に処理溶液中の過酸化水素の濃度の
経時変化を示す。Table 1 and FIG. 1 show changes in the concentration of hydrogen peroxide in the treatment solution over time.
また、同時にレンズを（各一定時間後に１セツトずつ）
取り出し、これを１ ｍｌの精製水中に移し、３０分間
放置し、レンズ中に残留していた過酸化水素を精製水中
に拡散させ、この溶液中の過酸化水素の濃度を日本薬局
方にしたがい測定することにより、コンタクトレンズ中
に残留していた過酸化水素の濃度（１セツトの平均値）
を求めた。つまり、レンズの含水率と含水時のレンズの
重量とからレンズ中の水分含量をあらかじめ求めておき
、これと精製水中に拡散した過酸化水素の濃度とがらコ
ンタクトレンズ中に残留していた過酸化水素の濃度（Ｗ
／Ｖ％）を求めた。第２表および第２図にコンタクトレ
ンズ中の過酸化水素の濃度の経時変化を示す。Also, remove the lenses at the same time (one set after each set period of time).
Remove the lens, transfer it to 1 ml of purified water, and leave it for 30 minutes to diffuse the hydrogen peroxide remaining in the lens into the purified water. Measure the concentration of hydrogen peroxide in this solution according to the Japanese Pharmacopoeia. By doing this, the concentration of hydrogen peroxide remaining in contact lenses (average value for one set)
I asked for In other words, the water content in the lens is calculated in advance from the water content of the lens and the weight of the lens when it contains water, and the hydrogen peroxide remaining in the contact lens is calculated by combining this with the concentration of hydrogen peroxide diffused in purified water. concentration (W
/V%) was determined. Table 2 and FIG. 2 show changes over time in the concentration of hydrogen peroxide in contact lenses.
該実施例１では、過酷条件として故意にコンタクトレン
ズ中に過酸化水素を完全に浸透させておいてから、過酸
化水素の分解を行なったが、それでもコンタクトレンズ
中の過酸化水素は確実に分解されていくことが第２表お
よび第２図から明らかとなった。In Example 1, the hydrogen peroxide was deliberately allowed to completely penetrate into the contact lens under severe conditions before decomposition of the hydrogen peroxide, but even so, the hydrogen peroxide in the contact lens was definitely decomposed. It is clear from Table 2 and Figure 2 that the
さらに、固定化した酵素から前記処理溶液中へ酵素タン
パク質が溶出していないがどぅがを調べるために、ロー
リ−（Ｌｏｖｒｙ）法にしたがい固定化酵素からのタン
パク質の溶出量を調べたところ、いずれもタンパク質の
溶出量は測定限界以下のため測定不能であった。つまり
、固定化酵素からは酵素タンパク質がほとんど溶出して
いないことがわかった。Furthermore, in order to investigate whether the enzyme protein was not eluted from the immobilized enzyme into the treatment solution, the amount of protein eluted from the immobilized enzyme was investigated according to the Lovry method. In both cases, the amount of protein eluted was below the measurement limit and could not be measured. In other words, it was found that almost no enzyme protein was eluted from the immobilized enzyme.
同様に、同じ操作を繰り返して測定を行なった。その結
果を第１表および第２表に示す。Similarly, measurements were performed by repeating the same operation. The results are shown in Tables 1 and 2.
［以下余白］
−１９一
実施例２（２液システム）
０．０１モルのリン酸緩衝液により約ｐＨ７に緩衝され
ると同時に等張化された３ Ｗ／Ｖ％濃度の過酸化水素
溶液を調製した。[Left below] -19 Example 2 (Two-liquid system) A hydrogen peroxide solution with a concentration of 3 W/V%, buffered to approximately pH 7 with a 0.01 molar phosphate buffer and made isotonic at the same time. Prepared.
約３０ｍ１容の小瓶を１０個用意し、それぞれの小瓶に
前記過酸化水素溶液を１． Ｏｍｌずつ入れた。各小瓶
の処理溶液中にソフトコンタクトレンズをそれぞれ１枚
入れ、２５℃の恒温水槽にて３０分間放置し、レンズ内
に過酸化水素を完全に浸透させた。Prepare 10 small bottles with a volume of about 30ml, and add 1.0% of the above hydrogen peroxide solution to each small bottle. I added Oml each. One soft contact lens was placed in the treatment solution in each vial and left in a constant temperature water bath at 25° C. for 30 minutes to allow hydrogen peroxide to completely penetrate into the lens.
これとは別に小瓶を１０個用意し、それぞれの小瓶に０
．０１モルのリン酸緩衝液により約ｐＨ７に緩衝された
生理食塩水をＬ Ｏｍｌずつ入れ、さらに前記参考例１
と同様にして作製した固定化酵素をそれぞれの小瓶に入
れておいた（２個の小瓶で１セツトとした）。Separately, prepare 10 small bottles and put 0 in each small bottle.
．． Add L Oml of physiological saline buffered to about pH 7 with 0.1M phosphate buffer, and then add
An immobilized enzyme prepared in the same manner as above was placed in each vial (one set consisted of two vials).
そして、前記過酸化水素溶液中からソフトコンタクトレ
ンズを取り出し、これら（レンズ１枚ずつ）をそれぞれ
固定化酵素を入れた水溶液中に移して、レンズに付着残
留していた過酸化水素を固定化酵素を入れた水溶液中に
拡散させて過酸化水素の分解反応を開始させた。Then, the soft contact lenses were removed from the hydrogen peroxide solution, and each of them (one lens at a time) was transferred to an aqueous solution containing immobilized enzyme, and the hydrogen peroxide remaining on the lenses was removed by the immobilized enzyme. The decomposition reaction of hydrogen peroxide was started by diffusing it into an aqueous solution containing hydrogen peroxide.
一定時間後、すなわち０分後、３分後、７分後、１５分
後および３０分後に１セツト（２個）ずつレンズを取り
出し、これを１ ｍｌの精製水中に移し、３０分間放置
し、レンズ中に残留していた過酸化水素を精製水中に拡
散させ、この溶液中の過酸化水素の濃度を日本薬局方に
したがい測定することにより、コンタクトレンズ中に残
留していた過酸化水素の濃度（Ｗ／Ｖ％）（１セツト（
２個）の平均値）を求めた。第３表および第３図にコン
タクトレンズ中の過酸化水素の濃度の経時変化を示す。After a certain period of time, that is, 0 minutes, 3 minutes, 7 minutes, 15 minutes, and 30 minutes, one set (two lenses) was taken out, transferred to 1 ml of purified water, and left for 30 minutes. The concentration of hydrogen peroxide remaining in contact lenses was determined by diffusing hydrogen peroxide remaining in lenses into purified water and measuring the concentration of hydrogen peroxide in this solution in accordance with the Japanese Pharmacopoeia. (W/V%) (1 set (
The average value of 2 pieces) was calculated. Table 3 and FIG. 3 show changes over time in the concentration of hydrogen peroxide in contact lenses.
該実施例２でも、過酷条件として故意にコンタクトレン
ズ中に過酸化水素を完全に浸透させておいてから、過酸
化水素の分解を行なったが、それでもコンタクトレンズ
中の過酸化水素は確実に分解されていくことが第３表お
よび第３図から明らかとなった。In Example 2, the hydrogen peroxide was intentionally allowed to completely penetrate into the contact lens under severe conditions before decomposition of the hydrogen peroxide, but even so, the hydrogen peroxide in the contact lens was definitely decomposed. It is clear from Table 3 and Figure 3 that the
また、固定化した酵素から前記０．０１モルのリン酸緩
衝液により約ｐ）１７に緩衝された生理食塩水中へ酵素
タンパク質が溶出していないかどうかを調べるために、
ローリ−（Ｌｏｖｒｙ）法にしたがい固定化酵素からの
タンパク質の溶出量を調べたところ、いずれもタンパク
質の溶出量は測定限界以下のため測定不能であった。つ
まり、固定化酵素からは酵素タンパク質かはとんと溶出
していないことがわかった。In addition, in order to examine whether the enzyme protein was eluted from the immobilized enzyme into the physiological saline buffered to approximately p17 with the 0.01 molar phosphate buffer,
When the amount of protein eluted from the immobilized enzyme was examined according to the Lovry method, it was impossible to measure the amount of protein eluted since it was below the measurement limit. In other words, it was found that the enzyme protein was not eluted from the immobilized enzyme.
［以下余白］
実施例３（直接接触システム）
０．０１モルのリン酸緩衝液により約１）Ｈ７に緩衝さ
れると同時に等張体された３ Ｗ／Ｖ％濃度の過酸化水
素溶液を調製した。[Left below] Example 3 (Direct Contact System) Preparing a hydrogen peroxide solution with a concentration of 3 W/V% that is approximately 1) H7 buffered and at the same time isotonic with a 0.01 molar phosphate buffer. did.
約３０ｍ１容の小瓶を同側用意し、各々の小瓶に前記過
酸化水素溶液を１０ｍ１ずつ入れた。各小瓶の処理溶液
中にソフトコンタクトレンズをそれぞれ１枚ずつ入れ、
２５℃の恒温水槽内に３０分間放置し、レンズ内に過酸
化水素を完全に浸透させた。Approximately 30 ml vials were prepared on the same side, and 10 ml of the hydrogen peroxide solution was placed in each vial. Place one soft contact lens into each vial of treatment solution.
The lens was left in a constant temperature water bath at 25° C. for 30 minutes to allow hydrogen peroxide to completely penetrate into the lens.
参考例１と同様にして作製した、シート状の固定化酵素
（寸法：縦３０＋ｎｍ Ｘ横３０Ｉ！１ｍＸ厚さ３ ｎ
＋ｍ）を１０個用意した。A sheet-shaped immobilized enzyme produced in the same manner as in Reference Example 1 (dimensions: length 30+nm x width 30I!1m x thickness 3n
+m) were prepared.
そして、前記過酸化水素溶液中からソフトコンタクトレ
ンズを取り出し、これら（レンズ１枚ずつ）をそれぞれ
のシート状の固定化酵素の中に挟み込んで（２枚のシー
トで１セツトとした）レンズと固定化酵素とを直接接触
させ、レンズに付着残留していた過酸化水素の分解反応
を開始させた。Then, the soft contact lenses were taken out of the hydrogen peroxide solution, and these (one lens at a time) were sandwiched between each sheet of immobilized enzyme (one set was made up of two sheets) and fixed with the lens. The lens was brought into direct contact with a hydrogen peroxide enzyme to initiate a decomposition reaction of hydrogen peroxide remaining on the lens.
一定時間後、すなわち０分後、１分後、２分後、３分後
および７分後に１セツト（２個）ずつレンズを取り出し
、これを１ ｍｌの精製水中に移し、３０分間放置し、
レンズ中に残留していた過酸化水素を精製水中に拡散さ
せ、この溶液中の過酸化水素の濃度を日本薬局方にした
がい測定することにより、コンタクトレンズ中に残留し
ていた過酸化水素の濃度（’ｄ／Ｖ％）（１セツトの平
均値）を求めた。第４表および第４図にコンタクトレン
ズ中の過酸化水素の濃度の経時変化を示す。After a certain period of time, that is, 0 minutes, 1 minute, 2 minutes, 3 minutes, and 7 minutes, one set (two lenses) was taken out, transferred to 1 ml of purified water, and left for 30 minutes.
The concentration of hydrogen peroxide remaining in contact lenses was determined by diffusing hydrogen peroxide remaining in lenses into purified water and measuring the concentration of hydrogen peroxide in this solution in accordance with the Japanese Pharmacopoeia. ('d/V%) (average value of one set) was determined. Table 4 and FIG. 4 show changes in the concentration of hydrogen peroxide in contact lenses over time.
該実施例３でも、過酷条件として故意にコンタクトレン
ズ中に過酸化水素を完全に浸透させておいてから、過酸
化水素の分解を行なったが、それでもコンタクトレンズ
中の過酸化水素は確実に分解されていくことが第４表お
よび第４図から明らかとなった。In Example 3, the hydrogen peroxide was intentionally allowed to completely penetrate into the contact lens under severe conditions before decomposition of the hydrogen peroxide, but even so, the hydrogen peroxide in the contact lens was definitely decomposed. It is clear from Table 4 and Figure 4 that the
また、固定化した酵素から酵素タンパク質がコンタクト
レンズに付着していないかどうかを調べるために、前記
過酸化水素を拡散させた精製氷１ ｍｌについてローリ
−（Ｌｏνｒｙ）法にしたがい固定化酵素からのタンパ
ク質の溶出量を調べたところ、いずれもタンパク質の溶
出量は測定限界以下のため測定不能であった。つまり、
固定化酵素からは酵素タンパク質がほとんど溶出してい
ないことがわかった。In addition, in order to check whether the enzyme protein from the immobilized enzyme was attached to the contact lens, 1 ml of the purified ice in which hydrogen peroxide had been diffused was mixed with the immobilized enzyme according to the Lovry method. When the amount of protein eluted was examined, it was impossible to measure the amount of protein eluted because it was below the measurement limit in both cases. In other words,
It was found that almost no enzyme protein was eluted from the immobilized enzyme.
［以下余白］
以」二の実施例から、過酸化水素の分解に用いる酵素が
固定化されていても、処理溶液中に存在する過酸化水素
およびコンタクトレンズ中に残留する過酸化水素は効率
的に分解されているのがわかった。一方、酵素は確実に
固定化されているので処理溶液中に溶出することはなか
った。[Blank below] From the second example, even if the enzyme used to decompose hydrogen peroxide is immobilized, the hydrogen peroxide present in the treatment solution and the hydrogen peroxide remaining in the contact lens can be efficiently decomposed. It was found that it was broken down into On the other hand, since the enzyme was reliably immobilized, it did not elute into the treatment solution.
［発明の効果］
本発明によれば、コンタクトレンズの消毒に用いた過酸
化水素を固定化酵素により効率的に分解することができ
る。一方酵素は固定化されているので処理溶液中に溶出
することはなく、酵素タンパク質がコンタクトレンズ表
面などに付着することはないという効果を奏する。[Effects of the Invention] According to the present invention, hydrogen peroxide used for disinfecting contact lenses can be efficiently decomposed by immobilized enzymes. On the other hand, since the enzyme is immobilized, it will not be eluted into the treatment solution, and the enzyme protein will not adhere to the surface of the contact lens.
したがって、過酸化水素の分解に使用した酵素に由来す
る酵素タンパク質を原因とする問題点、すなわちレンズ
が白濁したり消毒処理したコンタクトレンズを眼に装用
することによってアレルギー反応を起こすといった問題
点を解消することができる。Therefore, problems caused by enzyme proteins derived from the enzyme used to decompose hydrogen peroxide, such as clouding of lenses and allergic reactions caused by wearing disinfected contact lenses in the eyes, are eliminated. can do.
【図面の簡単な説明】
第１図は実施例１における処理溶液中の過酸化水素の濃
度の経時変化を示すグラフ、第２図 ・は実施例１に
おけるコンタクトレンズ中の過酸化水素の濃度の経時変
化を示すグラフ、第３図は実施例２におけるコンタクト
レンズ中の過酸化水素の濃度の経時変化を示すグラフ、
第４図は実施例３におけるコンタクトレンズ中の過酸化
水素の濃度の経時変化を示すグラフである。
壬；
才１図
時間（分）
第２図
時 間 （分）
Ａ′３図
時 間 （分）
才４図[Brief explanation of the drawings] Figure 1 is a graph showing the change in the concentration of hydrogen peroxide in the treatment solution in Example 1 over time, and Figure 2 shows the concentration of hydrogen peroxide in the contact lens in Example 1. A graph showing changes over time; FIG. 3 is a graph showing changes over time in the concentration of hydrogen peroxide in the contact lens in Example 2;
FIG. 4 is a graph showing the change over time in the concentration of hydrogen peroxide in the contact lens in Example 3. Figure 1: Time (minutes) Figure 2: Time (minutes) Figure A'3: Time (minutes) Figure 4: Time (minutes)
Claims (1)
において、消毒処理中もしくは消毒処理後に過酸化水素
を分解する手段として固定化酵素を用いることを特徴と
するコンタクトレンズの消毒方法。1. A method for disinfecting contact lenses using hydrogen peroxide, the method comprising using an immobilized enzyme as a means to decompose hydrogen peroxide during or after the disinfection process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62122133A JPH0698180B2 (en) | 1987-05-19 | 1987-05-19 | Contact lens disinfection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62122133A JPH0698180B2 (en) | 1987-05-19 | 1987-05-19 | Contact lens disinfection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63286158A true JPS63286158A (en) | 1988-11-22 |
| JPH0698180B2 JPH0698180B2 (en) | 1994-12-07 |
Family
ID=14828432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62122133A Expired - Fee Related JPH0698180B2 (en) | 1987-05-19 | 1987-05-19 | Contact lens disinfection method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0698180B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010500632A (en) * | 2006-08-16 | 2010-01-07 | ノバルティス アクチエンゲゼルシャフト | Enzymatic degradation of colorants in lens care solutions |
| WO2020100837A1 (en) * | 2018-11-12 | 2020-05-22 | 三菱瓦斯化学株式会社 | Composition for sterilization and cleaning, method for producing same, and sterilization and cleaning method employing same |
| WO2020261771A1 (en) * | 2019-06-26 | 2020-12-30 | 三菱瓦斯化学株式会社 | Peracetic acid composition for cleaning medical devices, and method for producing same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62114557A (en) * | 1985-11-08 | 1987-05-26 | ミネソタ マイニング アンド マニユフアクチユアリング コンパニ− | Article for neutralizing hydrogen peroxide by enzyme and disinfection method utilizing the same |
-
1987
- 1987-05-19 JP JP62122133A patent/JPH0698180B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62114557A (en) * | 1985-11-08 | 1987-05-26 | ミネソタ マイニング アンド マニユフアクチユアリング コンパニ− | Article for neutralizing hydrogen peroxide by enzyme and disinfection method utilizing the same |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010500632A (en) * | 2006-08-16 | 2010-01-07 | ノバルティス アクチエンゲゼルシャフト | Enzymatic degradation of colorants in lens care solutions |
| WO2020100837A1 (en) * | 2018-11-12 | 2020-05-22 | 三菱瓦斯化学株式会社 | Composition for sterilization and cleaning, method for producing same, and sterilization and cleaning method employing same |
| CN113039260A (en) * | 2018-11-12 | 2021-06-25 | 三菱瓦斯化学株式会社 | Composition for sterilization and cleaning, method for producing same, and method for sterilization and cleaning using same |
| CN113039260B (en) * | 2018-11-12 | 2023-06-06 | 三菱瓦斯化学株式会社 | Composition for sterilization and cleaning, method for producing same, and sterilization and cleaning method using same |
| TWI829800B (en) * | 2018-11-12 | 2024-01-21 | 日商三菱瓦斯化學股份有限公司 | Composition for sterilization and cleaning, method of manufacturing the composition, and sterilization and cleaning method using the composition |
| WO2020261771A1 (en) * | 2019-06-26 | 2020-12-30 | 三菱瓦斯化学株式会社 | Peracetic acid composition for cleaning medical devices, and method for producing same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0698180B2 (en) | 1994-12-07 |
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