JPS63248430A - Emulsifier composition - Google Patents

Abstract

PURPOSE:To further enhance the emulsifying capability of the title composition as compared with enzyme-treated lecithin and to appropriately apply the composition to food by mixing phospholipid such as enzyme-treated powdery lecithin and a monoglyceride to prepare the emulsifier composition. CONSTITUTION:The phospholipid such as enzyme-treated degummed oil cake, pasty lecithin, powdery lecithin, and refined high-purity lecithin and a monoglyceride are mixed to prepare the emulsifier composition. The phospholipid is commercially called lecithin, and classified into degummed oil cake, pasty lecithin obtained by dehydrating the oil cake and contg. a triglyceride, etc., in accordance with the purification degree. A monoglyceride contg. a saturated fatty acid is appropriately used as the monoglyceride. The content of the enzyme-treated phospholipid in the emulsifier is controlled to >=1wt.%, and the content of the monoglyceride is appropriately adjusted to the 1-10wt.% of the enzyme-treated phospholipid.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application of FAT The present invention relates to an emulsifier composition, and more particularly to an emulsifier composition containing enzyme-treated phospholipids and monoglycerides as active ingredients.

(b) Prior Art Lecithin is widely used in the food processing field as an emulsifier for stabilizing emulsification/foaming and promoting wetting. Recently, several modified lecithins made by making lecithin more hydrophilic or lipophilic have been developed, and further improvement in emulsifying power has been observed. Among them, enzyme-treated lecithin has particularly increased lecithin polarity and improved O/W emulsification ability, and is useful in the food processing field.

(C) Problems to be Solved by the Invention However, if the emulsifying ability of enzyme-treated lecithin can be further improved, it can be expected that it will become an even better emulsifier and a product of higher value.

An object of the present invention is to provide an emulsifier composition that has higher emulsifying ability than enzyme-treated lecithin.

(Means for solving the d1 problem) As a result of extensive research, the present inventors discovered that by adding monoglyceride to enzyme-treated phospholipids, the emulsifying ability of the phospholipids was dramatically improved.

That is, the present invention is an emulsifier composition containing an enzyme-treated phospholipid and a monoglyceride.

The phospholipid referred to in the present invention is commercially referred to as lecithin. Examples of lecithin include vegetable lecithin such as soybean lecithin, rapeseed lecithin, safflower lecithin, and sunflower lecithin, which are by-produced during the refining process of vegetable oil, and egg yolk lecithin. Among them the price,
Soybean lecithin is advantageous from the viewpoint of supply.

Depending on the degree of purification, lecithin can be made from degummed soapstock, paste lecithin containing triglycerides obtained by dehydrating it, powdered lecithin obtained by removing triglycerides by acetone fractionation, or phosphatidyl lecithin obtained by solvent or column fractionation. There is purified high-purity lecithin with increased choline content. In the present invention, any of these lecithins may be used. Examples of the enzyme used in the present invention include phospholibase A (hereinafter referred to as PL-A), which specifically hydrolyzes ester bonds in phospholipids.

The enzyme-treated phospholipid is 1% (by weight) in the emulsifier of the present invention.

The same applies hereafter) or more is desirable.

Furthermore, although not all phospholipids need to be enzyme-treated, enzyme-treated phospholipids make up 10% of all phospholipids.
It is preferable that the content is above.

When the amount of enzyme-treated phospholipid is less than the above amount, there is no substantial effect.

The monoglyceride used in the present invention is preferably a monoglyceride containing a saturated fatty acid from the viewpoint of emulsion stability, and for example, stearic acid monoglyceride can be used. Furthermore, monoglycerides of unsaturated fatty acids may be used depending on the purpose.

The monoglyceride may be a crude monoglyceride, a purified product, or one containing diglyceride or triglyceride.

The amount of monoglyceride is 1% or more, preferably about 10% of the amount of enzyme-treated phospholipid. When the amount is less than 1%, there is virtually no synergistic effect due to monoglyceride.

The enzyme-treated phospholipid of the present invention is produced, for example, as follows. That is, phospholipids are dispersed in water to form an emulsion.

Using an alkali such as sodium hydroxide, the optimum pH of PL-A is adjusted to the alkaline side, preferably pH 8 to 10. Furthermore, since PL-A is an enzyme that requires calcium ions, approximately 1 mM calcium chloride is added.

The amount of enzyme used is 1/1 in weight ratio to phospholipid.
00 to 1,150,000 (more preferably 1/
It is 1.000 to 1/10.000. Although the reaction temperature is preferably set to the optimum temperature for the enzyme, it is desirable to set the upper limit to 50° C. in order to prevent deterioration of lecithin due to heat.

The enzymatic reaction proceeds over a wide range of pH, but in order to prevent the fatty acid to be purified from adding to the pH during the reaction, an appropriate amount of an alkaline aqueous solution such as sodium hydroxide is added to maintain the optimum pH. The reaction time varies depending on the amount of enzyme used, pH, temperature, etc., but may be approximately 2 to 8 hours. The reaction is stopped by adding an appropriate amount of an acid such as hydrochloric acid to neutralize the pH. Thereafter, it is dried to obtain enzyme-treated phospholipids.

(e) Examples Example 1 Paste lecithin (Lecithin DX manufactured by Nisshin Oil Co., Ltd.) 4
Disperse 0g in 160g of warm water and emulsify with a homomixer. Add appropriate amount of IN sodium hydroxide solution to adjust pH to 8.
．． 0, and add 0.2 mj of 1M calcium chloride aqueous solution.
! Added. After heating to 50° C., 0.02 mj2 of PL-A (Lecitase 10 L manufactured by Novo Industries) was added, and the reaction was carried out with stirring. During the reaction, an appropriate amount of NaOH
Maintain the pH between 8.0 and 9.0 while adding the aqueous solution. After 6 hours, add an appropriate amount of hydrochloric acid to adjust the pH to 7.0.
Stop the reaction. Concentration and drying are performed to obtain 18 g of enzyme-treated lecithin.

Add 0.18 g of oleic acid monoglyceride (Emulgy MO manufactured by Riken Vitamin ■) and mix thoroughly to obtain a product.

This product has an acid value of 65, a peroxide value of 0, and an acetone insoluble content of 55%.

Example 2 From paste lecithin? Powdered soybean lecithin from which triglycerides have been removed using a container (PC- manufactured by Nisshin Oil Co., Ltd.)
30) Disperse 20g in 180g of warm water and suspend uniformly with a homomixer. Add an appropriate amount of IN potassium hydroxide aqueous solution to adjust the pH to 8.0, and add 0.2 mj! of 1M calcium chloride aqueous solution! Added.

After heating to 50° C., 0.2 g of pork pancreatin (Wako Pure Chemical Industries, Ltd. M) was added as an enzyme source for PL-A, and the reaction was carried out with stirring. During the reaction, the pH is maintained at 8.0 to 9.0 while adding an appropriate amount of IN potassium hydroxide aqueous solution. After 8 hours, hydrochloric acid was added to adjust the pH to 760 to complete the reaction.

After adding 0.2 g of stearic acid monoglyceride (Emulgy MS manufactured by Riken Vitamin A Kiku) and thoroughly stirring and mixing, the mixture is concentrated and dried to obtain a product.

This product has an acid value of 66, a peroxide value of 0, and an acetone insoluble content of 97%.

Example 3 20 g of purified high-purity soybean lecithin (PC-70 manufactured by Nisshin Oil Co., Ltd.) obtained by solvent and column fractionation treatment was added to 18 g of warm water.
0g and homogeneously suspended using a homomixer. IN
Add an appropriate amount of sodium hydroxide aqueous solution to adjust the pH to 8.0, and add 0.2 mJ of 1M calcium chloride aqueous solution. After heating to 50°C, 50 μ of PL-A (phospholipase AZ, manufactured by Sigma) was added, and the reaction was carried out with stirring. The pH is maintained between 8.0 and 9.0 while adding the same amount of IN sodium hydroxide solution during the reaction. After 4 hours, hydrochloric acid is added to adjust the pH to 7.0 to stop the reaction.

Add 0.2 g of safflower oil monoglyceride (Taiyo Kagaku Seisun Soft M8090) and stir and mix thoroughly.
After concentration and drying, 19 g of product is obtained.

This product has an acid value of 71, a peroxide value of 0, and an acetone insoluble content of 9.
It is 8%.

Experimental Examples When the emulsifier compositions prepared in Examples 1 to 3 were subjected to emulsification tests, the results shown in Table 1 were obtained.

The emulsifying power test was conducted as follows. 50 g of white soybean oil to which 0.2 g of the emulsifier composition was added is heated to 60°C. Water heated to 60℃ was heated to 600Orp using a homomixer.
While stirring at m, the soybean white squeezed oil containing an emulsifier was gradually added dropwise and emulsified at 6000 rpm for 5 minutes. emulsion 30
Transfer 1 ml to a graduated cylinder and let stand at room temperature. After 6 hours, the proportion of the emulsified layer is measured.

As a control, the emulsifying power of various samples not according to the present invention was similarly measured.

Table 1 Sample Emulsified layer ratio Enzyme-treated lecithin DX 10 Emulgy MO (Example 31 1)
981 Enzyme treatment PC-30 + Engineering Margy MS (
Example 2) 1001 Enzyme-treated PC-70 + Sunsoft Ma 8090 (Example 3) 100 Enzyme-treated Lecithin DX 54 Enzyme-treated PC-3071 Enzyme-treated PC-7073 Lecithin DX + Enzyme-treated Lecithin M0 62
PC-30 + Engineering Margy M3 6
BPC-70+Sunsoft floor 8090
70 lecithin DX
62PC-3066 DC-7867 (f) Effects of the Invention According to the present invention, an emulsifier composition with high emulsifying power can be obtained due to the synergistic effect of enzyme-treated phospholipids and monoglycerides. This effect cannot be predicted from enzyme-treated phospholipids alone or from a combination of non-enzyme-treated phospholipids and monoglycerides.

The emulsifier of the present invention is especially suitable for food use, and is characterized by excellent effects in pharmaceuticals, cosmetics, and other uses.Patent Applicant: Nisshin Oil Co., Ltd. Procedural Amendment (Voluntary) 1. Description of the case 1988 Patent Application No. 83553 2 Name of the invention Emulsifier composition 3 Person making the amendment Relationship to the case Patent applicant Address 23-1 Shinkawa-chome, Chuo-ku, Tokyo For correspondence regarding this case, please contact the following: please.

Postal code 221 Address 1-3 Chiwaka-cho, Kanayō-ku, Yokohama-shi, Kanagawa Prefecture
Name Nisshin Oil Co., Ltd. Laboratory type Story 0
45 (461)01204, Column 5 of Detailed Description of the Invention of the Specification Subject to Amendment, Contents of Amendment (1) The following sentence is inserted next to line 11 on page 4 of the specification.

``These may be commercially available synthetic products, or they may be used, for example, by hydrolyzing fats and oils.In the latter case, for example, after adding 1710 to 1 times the amount of water to fats and oils and suspending them, , weight ratio 1/100 to 1/100.000.
Preferably, 115,000 to 1/10,000 of the amount of lipase is added and reacted at 50° C. for 0.5 to 2 hours to partially hydrolyze the ester bonds. The oils and fats used are preferably vegetable oils such as soybean white squeezed oil, rapeseed white squeezed oil, and safflower white squeezed oil. (2) Insert the following sentence after line 10 on page 8.

"Example 4 20 g of PC-30 used in Example 2 is dispersed in 180 g of warm water and suspended uniformly using a homomixer.

Add appropriate amount of IN sodium hydroxide aqueous solution to adjust pH to 8.0.
and add 0.2 mg of 1M calcium chloride aqueous solution.

After heating to 50°C, 4 mg of phospholipase At (manufactured by Sigma) is added, and the reaction is carried out with stirring.

While adding the same amount of sodium hydroxide aqueous solution during the reaction, adjust the pH.
Maintain between 8.0 and 9.0. After 4 hours, add an appropriate amount of hydrochloric acid to adjust the pH to 7.0 and stop the reaction. Separately, add 0.2 g of water to 2 g of white soybean oil and suspend thoroughly using a homogenizer. After heating to 50°C, 1 mg of lipase (Lipase OF, manufactured by Meito Sangyo) is added, and reaction is carried out for 2 hours while stirring.

After the reaction, it is mixed with the former, concentrated and dried to obtain 20 g of product.

This product has an acid value of 75 and an acetone insoluble content of 50%. ”
(3) Change “Examples 1 to 3” to “Examples 1 to 3” on page 8, line 12.
4” is corrected.

(4) Table 1 on page 9 is corrected as follows.

" Table 1 Procedural Amendment 4゜ 10 Kawa 5, 1930 ζ 2. Name of the invention Emulsifier composition 3. Relationship to the person making the amendment Patent applicant's address 23-1 Shinkawa-chome, Chuo-ku, Tokyo Please contact us below regarding this matter.

Postal code 221 Address 1-3 Chiwaka-cho, Kanayō-ku, Yokohama-shi, Kanagawa Prefecture Target of amendment (11 Contents of amendment in the column of detailed description of the invention in the specification (11 Change ``beat'' to page 5, line 8 of the specification) Quite a bit,” he corrected.

(2) On page 5, line 9, ``purification'' is corrected to ``production.''

(3) On page 5, line 9, "addition" is corrected to "reduction."

Claims (2)

[Claims] (1) An emulsifier composition containing an enzyme-treated phospholipid and a monoglyceride. (2) The emulsifier composition according to claim 1, which uses degummed soapstock, paste lecithin, powdered lecithin, or purified high-purity lecithin as the phospholipid.