JPS63245661A - Bioreactor - Google Patents
BioreactorInfo
- Publication number
- JPS63245661A JPS63245661A JP8075787A JP8075787A JPS63245661A JP S63245661 A JPS63245661 A JP S63245661A JP 8075787 A JP8075787 A JP 8075787A JP 8075787 A JP8075787 A JP 8075787A JP S63245661 A JPS63245661 A JP S63245661A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- channel
- cylinder
- microorganism
- cylindrical body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 244000005700 microbiome Species 0.000 claims abstract description 24
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims description 19
- 238000010979 pH adjustment Methods 0.000 claims description 3
- 150000007524 organic acids Chemical class 0.000 abstract description 11
- 229910052751 metal Inorganic materials 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract description 4
- 238000007599 discharging Methods 0.000 abstract description 3
- 241000194032 Enterococcus faecalis Species 0.000 abstract description 2
- 235000010418 carrageenan Nutrition 0.000 abstract description 2
- 239000000679 carrageenan Substances 0.000 abstract description 2
- 229920001525 carrageenan Polymers 0.000 abstract description 2
- 229940113118 carrageenan Drugs 0.000 abstract description 2
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract 1
- 238000004080 punching Methods 0.000 abstract 1
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 18
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 235000005985 organic acids Nutrition 0.000 description 6
- 239000002253 acid Substances 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229940111688 monobasic potassium phosphate Drugs 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
（イ）産業上の利用分野
この発明は、バイオリアクターに関する。さらに詳しく
は、微生物、酵素等の特異的機能を応用して、食品素材
、医薬品等の各種有用有機物質を簡便にかつ連続的に産
出しうるバイオリアクター（生物反応器）に関する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to a bioreactor. More specifically, the present invention relates to a bioreactor that can easily and continuously produce various useful organic substances such as food materials and pharmaceuticals by applying the specific functions of microorganisms, enzymes, etc.
（ロ）従来の技術
乳酸、クエン酸、酢酸、グルコン酸、こうじ酸、ケトグ
ルタル酸、イタコン酸、プロピオン酸等の種々の有機酸
を製造する方法のうち、生物学的な方法として従来から
、原料液をバッチ式の発酵槽に入れて行なういわゆる発
酵法や、適当な担体に物理吸着や包括固定させた微生物
や酵素を容器に保持して原料液を接触させるいわゆるバ
イオリアクターによる方法が知られている。(b) Conventional technology Among the methods for producing various organic acids such as lactic acid, citric acid, acetic acid, gluconic acid, kojic acid, ketoglutaric acid, itaconic acid, and propionic acid, biological methods have traditionally used raw materials. The so-called fermentation method, in which the liquid is placed in a batch-type fermenter, and the so-called bioreactor method, in which microorganisms and enzymes physically adsorbed or entrappingly immobilized on a suitable carrier are held in a container and brought into contact with the raw material liquid, are known. There is.
（ハ）発明が解決しようとする問題点
しかし、前者の発酵法においては、産出される有１酸が
微生物や酵素自体を阻害し、場合によっては微生物の死
滅や酵素の失活を招く問題点があった。そのため、発酵
槽内に生成した有機酸を中和すべく一調整剤を添加して
おくことが行なわれているが、通常、発酵の至適−より
も高くなるため、煩雑であると共に、有機酸の産生効率
が低］：する不都合があった。(c) Problems to be solved by the invention However, in the former fermentation method, the monoacid produced inhibits the microorganisms and enzymes themselves, and in some cases, the problem is that the microorganisms may be killed or the enzymes may be inactivated. was there. For this reason, a regulator is added to neutralize the organic acids generated in the fermenter, but this is usually more expensive than the optimum level for fermentation, which is complicated and Acid production efficiency is low]: There was an inconvenience.
これに対し、後者のバイオリアクターを用いた方法にお
いては、例えば微生物や酵素の保持容器を通過させるプ
ラグフロー型バイオリアクターの場合、原料液の導入側
では比較的速かな有機酸の産生が行なわれるものの、排
出側では生成された有８１ＩＭの濃縮により一の低下が
生じ、発酵法と同様に微生物や酵素の活性が低下して産
生効率が著しく低下する問題があった。On the other hand, in the latter method using a bioreactor, for example, in the case of a plug-flow bioreactor that passes through a holding container for microorganisms or enzymes, organic acids are produced relatively quickly on the introduction side of the raw material liquid. However, on the discharge side, there was a problem in that the concentration of the produced IM was reduced by 1, and as with the fermentation method, the activity of microorganisms and enzymes was reduced, resulting in a significant reduction in production efficiency.
この発明は、かかる状況に鑑みなされたものであり、産
生じた有機酸による微生物や酵素への弊害を防止できる
バイオリアクターを提供しようとするものである。The present invention was made in view of this situation, and aims to provide a bioreactor that can prevent the harmful effects of produced organic acids on microorganisms and enzymes.
（ニ）問題点を解決するための手段
かくしてこの発明によれば液透過性の多孔質内部筒状体
と液不透過性の外部筒状体とからなる二重筒体から構成
され、上記内部筒状体内に微生物、酸素等を保持すると
共に該筒状体に液導入口及び液排出口を設けて原料液変
換流路を構成し、かつ上記外部筒状体に液導入口及び液
排出口を設けて上記内部筒状体内のＰＩＩ調整用流路を
構成したことを特徴とするバイオリアクターが提供され
る。(d) Means for Solving the Problems Thus, according to the present invention, the double cylindrical body is composed of a liquid-permeable porous inner cylindrical body and a liquid-impermeable outer cylindrical body. Microorganisms, oxygen, etc. are held in the cylindrical body, and a liquid inlet and a liquid outlet are provided in the cylindrical body to constitute a raw material liquid conversion flow path, and a liquid inlet and a liquid outlet are provided in the external cylindrical body. Provided is a bioreactor characterized in that a PII adjustment flow path in the internal cylindrical body is configured by providing a PII adjusting flow path in the internal cylindrical body.
この発明の最も特徴とする点は、微生物、酵素等の保持
容器の周囲に、該保持容器内の−を調整しうる流路を設
けた点であり、それを可能とすべく反応器を内部が多孔
質筒状体からなる二重筒体で構成した点にある。The most distinctive feature of this invention is that a flow path is provided around the container for holding microorganisms, enzymes, etc., so that the inside of the container can be adjusted. The structure consists of a double cylinder made of porous cylinders.
上記多孔質内部筒状体は、液透過性でかつ保持される微
生物や酵素を外部へ逸散させない程度の多孔質材からな
る筒状体が用いられる。ただし、微生物や酵素の保持は
前述のごと（適肖な担体く例えば、カラーギナンゲル、
アルギン酸ゲル、シリカゲル、ポーラスガラス等′；通
常、１０〜１２０メツシユのもの）に吸着や固定して筒
状体内に充填・分散させることにより通常行なわれるた
め、この多孔度はこれらの担体が通過しない程度の粗い
ものであってもよい。かかる多孔質材としては、例えば
パンチングメタル、焼結金属、連続気泡を有する合成樹
脂多孔体、ガラスフィルター材等が挙げられ、これらの
組合せであってもよい。The porous inner cylindrical body used is a cylindrical body made of a porous material that is permeable to liquid and does not allow the retained microorganisms and enzymes to escape to the outside. However, retention of microorganisms and enzymes is as described above (e.g., using suitable carriers such as carraginan gel,
This is usually done by adsorbing or fixing on alginate gel, silica gel, porous glass, etc.' (usually 10 to 120 mesh) and filling and dispersing it in a cylindrical body, so this porosity prevents these carriers from passing through. It may be rough. Examples of such porous materials include punched metals, sintered metals, porous synthetic resins having open cells, glass filter materials, and combinations thereof.
また担体等を介して保持される微生物や酵素は、前述し
たような意図する有機酸を産生じうるものが選択され、
当該分野で用いられるものが適宜使用できる。In addition, the microorganisms and enzymes retained via a carrier etc. are selected from those capable of producing the intended organic acid as described above.
Those used in the field can be used as appropriate.
この発明における内部筒状体には原料液を導入し、かつ
産生有機酸含有液を排出するための液導入口及び液排出
口が設けられ、通常これらは筒状体の両端に設けられる
。これにより原料液変換流路が構成される。The internal cylindrical body of the present invention is provided with a liquid inlet and a liquid outlet for introducing the raw material liquid and discharging the produced organic acid-containing liquid, and these are usually provided at both ends of the cylindrical body. This constitutes a raw material liquid conversion channel.
一方、外部筒状体にはｐＨ調整液を流すための液導入口
及び液排出口が設けられ、通常、これらも両端に設けら
れる。これにより一調整用流路が構成される。ここで−
調整液としては、弱アルカリ水溶液又は−緩衝液が適し
ており、前者の場合は例えば炭酸カルシウムのようなア
ルカリ土類金属の弱酸塩や炭酸水素ナトリウムのような
アルカリ金属の酸性塩等が挙げられ、後者の場合は微生
物や酵素の至適−に一致又は近接した緩衝液、例えばリ
ン酸バッファーのごとき中性緩衝液等が挙げられる。か
かるｐＨＩ！整液を孔質る場合、−調整用流路の液圧を
原料液変換流路の液圧に比して高くすることが好ましい
。これによりＰ１１ｗＡ整液が多孔質の内部筒状体を介
して原料液変換流路に連続して導入され、その有機酸中
和作用に基づき該変換流路内、ことに排出口側の−の低
下が防止されることとなる。On the other hand, the external cylindrical body is provided with a liquid inlet and a liquid outlet for flowing the pH adjusting liquid, and these are usually also provided at both ends. This constitutes one adjustment flow path. Here-
As the adjustment solution, a weak alkaline aqueous solution or a buffer solution is suitable; in the case of the former, examples include weak acid salts of alkaline earth metals such as calcium carbonate and acid salts of alkali metals such as sodium hydrogen carbonate. In the latter case, a buffer solution matching or close to the optimum of the microorganism or enzyme, for example, a neutral buffer solution such as a phosphate buffer, etc. may be used. That pHI! When the liquid regulating channel is made porous, it is preferable that the hydraulic pressure in the -adjustment channel be higher than the hydraulic pressure in the raw material liquid conversion channel. As a result, the P11wA liquid conditioner is continuously introduced into the raw material liquid conversion channel through the porous internal cylindrical body, and based on its organic acid neutralizing effect, the - in the conversion channel, especially on the outlet side. This will prevent a decrease in the temperature.
ただし、上記−調整液としては水自体を用いることも可
能である。この場合には、原料変換流路の液圧を一５Ｉ
ｍ用流路の液圧に比して大きくすることが好ましい。こ
れにより産生された有機酸の一部が多孔質の内部筒状体
を介してｐＨＩＩＩ用流路へ通流路て原料変換流路内、
ことに排出口側の−の低下が防止されることとなる。However, it is also possible to use water itself as the above-mentioned adjustment liquid. In this case, the liquid pressure in the raw material conversion flow path is set to -5I.
It is preferable to make the pressure higher than the hydraulic pressure of the flow path for m. A part of the organic acid produced by this passes through the porous internal cylindrical body to the pHIII flow path, and enters the raw material conversion flow path.
In particular, a decrease in - on the discharge port side is prevented.
（ホ）作用
ｖＨ調整用流路に一調孔質を流すことにより、原料液変
換流路内の有りｌ酸産生に基づく一低下が防止又は緩和
されることとなる。(E) Effect: By flowing a uniformly porous material through the VH adjustment flow path, a drop in the content due to acid production in the raw material liquid conversion flow path can be prevented or alleviated.
（へ）実施例
第１図は、この発明のバイオリアクターの一実施例を示
す構成説明図である。図において、バイオリアクター１
は、パンチングメタルからなる多孔質内部円筒体２とス
テンレスからなる外部円筒体３による二ｍ円筒管で構成
され、この内部円筒体２内には、ストレプトコッカス・
フエーカリス（Ｓｔ 、 ｆａｅｃａｌｉｓ）をカラギ
ーナンで包括固定した固定化微生物５が充填されて微生
物保持層４が形成されてなる。そして内部円筒体２の一
端の液導入口６には原料液（１１；グルコース２％、ペ
プトン０．５％、イーストエキストラクト０．５％及び
第１リン酸カリウムからなるｐＨ６，５の乳酸産生用溶
液）の供給路が接続され、他端の液排出ロアには排出路
が接続され、これにより、微生物保持層４は原料液変換
流路を構成する。一方、外部円筒体３の液導入口８には
ｐＨ１２の炭酸カルシウム水溶液（１３：ｐ＋４調整液
）の供給路が接続され、液排出口９には排出、路が接続
され、これにより内部円筒体２の外周にＰＨ調整用流路
１０が構成されている。そして炭酸カルシウム水溶液１
３の供給圧は０．１ｋｇ＜Ｑとされ、原料液の供給圧０
．０５ ｋＧ！４に比してやや高目に設定されている。(f) Example FIG. 1 is an explanatory diagram of the configuration of an example of the bioreactor of the present invention. In the figure, bioreactor 1
consists of a 2 m cylindrical tube consisting of a porous inner cylinder 2 made of punched metal and an outer cylinder 3 made of stainless steel.
A microorganism holding layer 4 is formed by filling an immobilized microorganism 5 in which St. faecalis is comprehensively immobilized with carrageenan. The liquid inlet 6 at one end of the internal cylindrical body 2 has a raw material liquid (11; lactic acid production with pH 6.5 consisting of 2% glucose, 0.5% peptone, 0.5% yeast extract, and monobasic potassium phosphate). A supply path for a solution (for use in water) is connected thereto, and a discharge path is connected to the liquid discharge lower at the other end, whereby the microorganism holding layer 4 constitutes a raw material liquid conversion flow path. On the other hand, a supply path for a calcium carbonate aqueous solution (13:p+4 adjustment liquid) with a pH of 12 is connected to the liquid inlet 8 of the external cylinder 3, and a discharge path is connected to the liquid discharge port 9. A PH adjustment flow path 10 is configured on the outer periphery of 2. and calcium carbonate aqueous solution 1
The supply pressure of No. 3 is 0.1kg<Q, and the supply pressure of the raw material liquid is 0.
．． 05 kG! It is set slightly higher than 4.
なお、図中、１４は固定化微生物保持用の金網を示す。In addition, in the figure, 14 indicates a wire mesh for holding immobilized microorganisms.
また、二重円筒管、供給配管、原料液等はいずれもオー
トクレーブで１２０℃下、１５分間滅菌されたものを用
いた。Further, the double cylindrical tube, supply piping, raw material liquid, etc. were all sterilized in an autoclave at 120° C. for 15 minutes.
かかるバイオリアクター１において、原料液１１を供給
することにより、微生物保持層４内でストレプトコッカ
ス・フエーカリスの代謝作用に基づいてＬ−乳酸が産生
されその含有液１２が得られるが、この場合、保持層４
内には一調整用流路１０から多孔質の内部円筒体２を介
してアルカリ性の炭酸カルシウム水溶液が供給されてい
るため、Ｌ−乳酸は速かに中和され、保持１１４内、こ
とに液排出ロア側付近の−の低下が防止される。In this bioreactor 1, by supplying the raw material liquid 11, L-lactic acid is produced based on the metabolic action of Streptococcus faecalis in the microorganism holding layer 4, and a liquid containing L-lactic acid 12 is obtained. 4
Since an alkaline calcium carbonate aqueous solution is supplied into the interior of the holding chamber 114 through the porous internal cylinder 2, L-lactic acid is quickly neutralized, and the liquid inside the holding chamber 114, especially the liquid, is quickly neutralized. A decrease in - near the discharge lower side is prevented.
従って、保持される微生物の活性低下が長期勘に屋って
防止され、Ｌ−乳酸を効率良く産生ずることができる。Therefore, a decrease in the activity of the retained microorganisms is prevented over a long period of time, and L-lactic acid can be efficiently produced.
一方、第２図は、この発明の他の実施例を示すものであ
り、内部円筒体２として金網で支持されたステンレス製
フィルタ（メツシュ５０〜１００）を用い、−調整液と
して滅菌した再蒸留水１５を用い、また原料液の供給圧
が０．１ｋｇ４で再蒸留水の供給圧０．０５ ｋａｄよ
りもやや高目に設定する以外、第１図と同様に構成した
ものである。On the other hand, FIG. 2 shows another embodiment of the present invention, in which a stainless steel filter (mesh 50 to 100) supported by a wire mesh is used as the inner cylindrical body 2; The structure was the same as that shown in FIG. 1, except that water 15 was used and the supply pressure of the raw material liquid was set at 0.1 kg4, which was slightly higher than the supply pressure of redistilled water, 0.05 kad.
かかるバイオリアクター１′においては、保持層４内で
産生ずるＬ−乳酸の一部が、内部円筒体２を介してｐＨ
調整用流路１０内に移行されるため、保持層４内ことに
液排出ロア側付近の１低下が緩和され、Ｌ−乳酸を長期
間に亙っで効率良く賛成することができる。In such a bioreactor 1', a part of L-lactic acid produced in the retention layer 4 passes through the internal cylindrical body 2 to pH
Since the L-lactic acid is transferred into the adjustment flow path 10, the drop in the content of the holding layer 4, especially near the liquid discharge lower side, is alleviated, and L-lactic acid can be efficiently supplied for a long period of time.
なお、第２図の構成のバイオリアクターによれば乳酸以
外の原料液内の高分子成分（例えばポリペプトン、イー
ストエキストラクト等の一部も−調整用流路１０内に分
画排出されるため、精製がより容易なＬ−乳酸含有液１
２を得ることができる利点がある。また、−調整排液１
６からさらにＬ−乳酸を分離精製することも可能である
。In addition, according to the bioreactor configured as shown in FIG. 2, part of the polymer components (for example, polypeptone, yeast extract, etc.) in the raw material solution other than lactic acid are also fractionated and discharged into the adjustment channel 10. L-lactic acid-containing liquid 1 that is easier to purify
There is an advantage that you can obtain 2. In addition, -adjusted drainage liquid 1
It is also possible to further separate and purify L-lactic acid from 6.
（ト）発明の効果
この発明のバイオリアクターによれば、微生物保持層の
１低下を防止又は緩和しつつ、有機酸を長期間効率良く
産生ずることができる。従って、種々の有機酸の生物学
的に製造する装置として極めて有用なものである。(G) Effects of the Invention According to the bioreactor of the present invention, it is possible to efficiently produce organic acids for a long period of time while preventing or alleviating a decrease in the microbial retention layer. Therefore, it is extremely useful as a device for biologically producing various organic acids.
第１図及び第２図は、各々この発明のバイオリアクター
の一実施例を示す構成説明図である。
１．１−、・・・・・・バイオリアクター、２・・・・
・・多孔質内部円筒体、
３・・・・・・外部円筒体、 ４・・・・・・微生物保
持層、５・・・・・・固定化微生物、６．８・・・・・
・液導入口、７．９・・・・・・液排出口、
１０・・・・・・ｐＨ調整用流路。
第 １ 図
第 ２ 図FIG. 1 and FIG. 2 are configuration explanatory diagrams each showing an embodiment of the bioreactor of the present invention. 1.1-,...Bioreactor, 2...
... Porous internal cylinder, 3 ... External cylinder, 4 ... Microorganism retention layer, 5 ... Immobilized microorganism, 6.8 ...
-Liquid inlet, 7.9...liquid outlet, 10...channel for pH adjustment. Figure 1 Figure 2
Claims (1)
筒状体とからなる二重筒体から構成され、上記内部筒状
体内に微生物、酵素等を保持すると共に該筒状体に液導
入口及び液排出口を設けて原料液変換流路を構成し、か
つ上記外部筒状体に液導入口及び液排出口を設けて上記
内部筒状体内のｐＨ調整用流路を構成したことを特徴と
するバイオリアクター。(1) It is composed of a double cylinder consisting of a liquid-permeable porous inner cylinder and a liquid-impermeable outer cylinder, and the cylinder holds microorganisms, enzymes, etc. in the internal cylinder. A liquid inlet and a liquid outlet are provided in the shaped body to constitute a raw material liquid conversion flow path, and a liquid inlet and a liquid outlet are provided in the outer cylindrical body to form a pH adjustment flow path in the inner cylindrical body. A bioreactor characterized by comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8075787A JPS63245661A (en) | 1987-03-31 | 1987-03-31 | Bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8075787A JPS63245661A (en) | 1987-03-31 | 1987-03-31 | Bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63245661A true JPS63245661A (en) | 1988-10-12 |
Family
ID=13727282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8075787A Pending JPS63245661A (en) | 1987-03-31 | 1987-03-31 | Bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63245661A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018527591A (en) * | 2015-09-01 | 2018-09-20 | ローズマウント・アナリティカル・インコーポレーテッドＲｏｓｅｍｏｕｎｔ Ａｎａｌｙｔｉｃａｌ Ｉｎｃ． | Single-use pH sensor storage solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6170976A (en) * | 1984-09-14 | 1986-04-11 | Nitto Electric Ind Co Ltd | Biochemical reactor |
-
1987
- 1987-03-31 JP JP8075787A patent/JPS63245661A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6170976A (en) * | 1984-09-14 | 1986-04-11 | Nitto Electric Ind Co Ltd | Biochemical reactor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018527591A (en) * | 2015-09-01 | 2018-09-20 | ローズマウント・アナリティカル・インコーポレーテッドＲｏｓｅｍｏｕｎｔ Ａｎａｌｙｔｉｃａｌ Ｉｎｃ． | Single-use pH sensor storage solution |
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