JPS632343B2 - - Google Patents
Info
- Publication number
- JPS632343B2 JPS632343B2 JP14462380A JP14462380A JPS632343B2 JP S632343 B2 JPS632343 B2 JP S632343B2 JP 14462380 A JP14462380 A JP 14462380A JP 14462380 A JP14462380 A JP 14462380A JP S632343 B2 JPS632343 B2 JP S632343B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- protease
- measured
- cleaning
- ions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000004140 cleaning Methods 0.000 claims description 22
- 239000004365 Protease Substances 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 18
- 108091005804 Peptidases Proteins 0.000 claims description 17
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 229920005597 polymer membrane Polymers 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 210000002966 serum Anatomy 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 108091005658 Basic proteases Proteins 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000008280 blood Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 238000005259 measurement Methods 0.000 description 11
- 235000019419 proteases Nutrition 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 239000012086 standard solution Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 229910001414 potassium ion Inorganic materials 0.000 description 6
- 229910001415 sodium ion Inorganic materials 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 102000035195 Peptidases Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 4
- 239000004327 boric acid Substances 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- -1 NaHCO 3 Inorganic materials 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 108010004032 Bromelains Proteins 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000019835 bromelain Nutrition 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000010200 folin Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000021962 pH elevation Effects 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/38—Cleaning of electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
本発明は、生体成分中の物質を測定するための
高分子膜電極の洗浄液に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cleaning solution for a polymer membrane electrode for measuring substances in biological components.
近年、医学の進歩につれて、科学的診断を下す
ために、血液、尿などを分析することは常識とな
つている。これらの分析手段としては、化学分析
ばかりでなく、炎光分析などの物理的手段も用い
られるようになつてきた。なかでも種々の高分子
膜電極を用いる方法は、電極を検体に浸すだけで
測定が行なわれるので操作が簡単であり、急速に
発展しつつある。 In recent years, as medical science has progressed, it has become common knowledge to analyze blood, urine, etc. in order to make a scientific diagnosis. As these analytical means, not only chemical analysis but also physical means such as flame analysis have come to be used. Among these methods, methods using various polymer membrane electrodes are easy to operate because measurements are performed simply by dipping the electrodes into the sample, and are rapidly developing.
しかし、血液や尿などの生体成分は、種々の蛋
白質を含有しているため、これらが電極膜に付着
し、測定を繰返しているうちに感度が低下して、
ついに測定不能となる。 However, since biological components such as blood and urine contain various proteins, these adhere to the electrode membrane and the sensitivity decreases as measurements are repeated.
Eventually it becomes impossible to measure.
そこで、検体の測定の間に、電極を洗浄するこ
とが考えられるが、単なる水洗では電極に付着し
た蛋白質を除去することができない。また、界面
活性剤を含んだ洗剤で洗浄することが考えられる
が、界面活性剤が電極の高分子膜に作用して、そ
の特性を変化させたり、通常の洗剤に含まれてい
る硫酸ナトリウムがナトリウムイオン、カリウム
イオンを測定する場合、その測定値に影響を与え
好ましくない。 Therefore, it may be possible to wash the electrodes between specimen measurements, but simply washing with water cannot remove proteins attached to the electrodes. Another possibility is cleaning with a detergent containing a surfactant, but the surfactant may act on the polymer membrane of the electrode and change its properties, or the sodium sulfate contained in ordinary detergents may When measuring sodium ions and potassium ions, this is undesirable as it affects the measured values.
本発明は、これらの問題点を解決するものであ
る。 The present invention solves these problems.
すなわち、本発明は、生体成分中の物質を測定
するための高分子膜電極の洗浄液であつて、水お
よびプロテアーゼを含有してなる洗浄液に関す
る。 That is, the present invention relates to a cleaning liquid for a polymer membrane electrode for measuring substances in biological components, which comprises water and protease.
生体成分中の物質を高分子膜電極を使用して測
定するには、まず、測定物質を所定濃度で溶解す
る標準液を２個以上用意し、これに上記電極を浸
漬し、電位差を測定して電位差と濃度の相関関係
を調べる。すなわち、検量線を設定する。この
後、生体成分（検体）または多くの場合その一定
倍率の希釈液に電極を浸漬して電位差を測定し、
これを上記検量線により照合して測定物質の濃度
を決定する。すなわち、測定物質の上記生体成分
中の濃度を測定する。この後電極は較正のため
に、内部標準液に浸漬され、電位の誤差を修正す
る。つぎに、上記と同様に検体の測定と較正が繰
返される。この場合、必要に応じ検体の測定の前
後で電極は洗浄される。 To measure a substance in a biological component using a polymer membrane electrode, first prepare two or more standard solutions in which the substance to be measured is dissolved at a predetermined concentration, immerse the electrode in this solution, and measure the potential difference. to investigate the correlation between potential difference and concentration. That is, a calibration curve is set. After this, the electrode is immersed in a biological component (specimen) or in many cases a diluted solution of a certain dilution, and the potential difference is measured.
This is compared with the above calibration curve to determine the concentration of the substance to be measured. That is, the concentration of the substance to be measured in the biological component is measured. The electrodes are then immersed in an internal standard solution for calibration to correct potential errors. Next, the sample measurement and calibration are repeated in the same manner as above. In this case, the electrodes are cleaned before and after measuring the specimen as necessary.
また、検量線を設定後、検体の測定と電極の洗
浄を繰返し、時々内部標準液で較正される。本発
明に係る洗浄液は、上記におけるような洗浄のた
めに使用される。また、本発明に係る洗浄液は使
用後の電極の洗浄等に使用される。使用後の電極
の洗浄には、該電極を本発明に係る洗浄液に浸漬
しておく方法を含む。 In addition, after setting the calibration curve, sample measurement and electrode cleaning are repeated, and calibration is sometimes performed with an internal standard solution. The cleaning liquid according to the invention is used for cleaning as described above. Further, the cleaning liquid according to the present invention is used for cleaning electrodes after use. Cleaning of the electrode after use includes a method of immersing the electrode in a cleaning solution according to the present invention.
この洗浄液は水およびプロテアーゼを必須成分
として含むものである。 This cleaning solution contains water and protease as essential components.
上記プロテアーゼとしては、バクテリアの生産
するプロテアーゼ、放線菌の生産するプロテアー
ゼ、糸状菌の生産するプロテアーゼ、ペプシン、
キモトリプシン、パパイン、ブロメライン等種々
ある。これらは、測定時の誤差をなくすために検
体、測定されるべき物質、電極等を考慮して、ま
た、検体のPH等を考慮して、適宜選択して使用さ
れるのが好ましい。また同様に検体が血清、尿な
どであり、カリウムイオン、ナトリウムイオン、
塩素イオンなどを測定し、液膜型電極を使用する
場合、アルカリ性化で活性なアルカリ性プロテア
ーゼ特に、バクテリア生産のアルカリ性プロテア
ーゼが好ましい。 The proteases mentioned above include proteases produced by bacteria, proteases produced by actinomycetes, proteases produced by filamentous fungi, pepsin,
There are various types such as chymotrypsin, papain, and bromelain. These are preferably selected and used as appropriate, taking into account the specimen, the substance to be measured, the electrodes, etc., and the pH of the specimen, etc., in order to eliminate errors during measurement. Similarly, the specimens are serum, urine, etc., and potassium ions, sodium ions, etc.
When measuring chloride ions or the like and using a liquid film electrode, alkaline proteases active in alkalinization, particularly alkaline proteases produced by bacteria, are preferred.
洗浄液中のプロテアーゼの濃度は５単位／ml以
上、好ましくは20〜150単位／mlである。５単
位／ml未満では電極に付着した蛋白質の除去効果
が小さい。なお、ここで単位とは、カゼイン−フ
オリン（Casein−Folin）呈色法（例えば、赤堀
四郎編：「酵素研究法２」第242頁〔朝倉書点発
行〕に記載される方法）で測定した力価である。 The concentration of protease in the washing solution is 5 units/ml or more, preferably 20 to 150 units/ml. If it is less than 5 units/ml, the effect of removing proteins attached to the electrode is small. Note that the unit here refers to the amount measured by the Casein-Folin coloring method (for example, the method described in "Enzyme Research Methods 2" edited by Shiro Akahori, p. 242 [published by Asakura Shoten]). It is the titer.
プロテアーゼには、その製造中にナトリウムイ
オン、カリウムイオン、塩素イオンなどが混在す
ることがあるが、これらが測定されるべき物質で
あるとき、混在するナトリウムイオンは、
0.003μEq／プロテアーゼ単位以下、混在するカ
リウムイオンは0.0002μEq／プロテアーゼ単位以
下、混在する塩素イオンは0.003μEq／プロテア
ーゼ単位以下のプロテアーゼが測定誤差をなくす
ために使用されるのが好ましい。 Sodium ions, potassium ions, chloride ions, etc. may be mixed into protease during its manufacture, but when these are the substances to be measured, the mixed sodium ions are
In order to eliminate measurement errors, it is preferable to use protease with an amount of 0.003 μEq/protease unit or less, a mixed potassium ion amount of 0.0002 μEq/protease unit or less, and a mixed chlorine ion amount of 0.003 μEq/protease unit or less.
その他、洗浄液には電極膜を変化させることの
あるリパーゼは混在しないか混在しても極微量で
あるようにされる。 In addition, lipase, which can change the electrode membrane, is not mixed in the cleaning solution, or even if it is mixed, it is kept in a very small amount.
洗浄液は必要に応じ、他の添加剤を適宜、添加
することができ、測定されるべき物質が混在して
もよい。しかし、測定誤差をなくすためにこのよ
うな余分な成分は、できるだけ混在しないように
する。 Other additives may be appropriately added to the cleaning liquid as necessary, and substances to be measured may be mixed therein. However, in order to eliminate measurement errors, such extra components should be avoided as much as possible.
本発明において生体成分とは、血液、血清、血
しよう、尿などである。これらは、このまま、ま
たは希釈液により希釈してから測定に供される。
希釈液としては、生体成分の測定物質の濃度測定
に悪影響を及ぼさないものが使用される。好まし
くは、緩衝液例えば、トリスヒドロキシメチルア
ミノメタンおよびホウ酸を含む水溶液などが使用
される。 In the present invention, biological components include blood, serum, blood plasma, urine, and the like. These are used for measurement as is or after being diluted with a diluent.
As the diluent, one is used that does not adversely affect the measurement of the concentration of the substance to be measured as a biological component. Preferably, a buffer is used, such as an aqueous solution containing trishydroxymethylaminomethane and boric acid.
生体成分中の測定されるべき物質としては、主
に、カリウムイオン、ナトリウムイオン、カルシ
ウムイオン、塩素イオン等であり、その他グルコ
ース、アンモニアなどもある。 The substances to be measured in biological components are mainly potassium ions, sodium ions, calcium ions, chloride ions, etc., and also glucose, ammonia, etc.
高分子膜電極とは、液膜形電極、酵素電極等の
高分子の感応膜を有する電極である。 A polymer membrane electrode is an electrode having a polymer sensitive membrane, such as a liquid membrane electrode or an enzyme electrode.
内部標準液は、水および測定されるべき物質と
同じ物質を必須成分として含み、場合により電
極、内部標準液自身に悪影響を及ぼさない緩衝
剤、殺菌剤などが含まれる。ここで緩衝剤として
は、トリスヒドロキシメチルアミノメタンとホウ
酸の系などがある。 The internal standard solution contains water and the same substance as the substance to be measured as essential components, and optionally contains electrodes, a buffer that does not adversely affect the internal standard solution itself, a sterilizing agent, and the like. Examples of buffering agents include trishydroxymethylaminomethane and boric acid.
内部標準液に含有される測定されるべき物質と
同一の物質とは上記したものであるが、その濃度
は、較正のために適した濃度に適宜調整される。
この場合、ナトリウムイオンのためにはNaCl、
NaHCO3、NaH2PO4等、カリウムイオンのため
にはKCl、KHCO3、KH2PO4等、塩素イオンの
ためには、NaCl、KCl等が使用される。 The same substance as the substance to be measured contained in the internal standard solution is the one described above, and its concentration is appropriately adjusted to a concentration suitable for calibration.
In this case, for sodium ions, NaCl,
NaHCO 3 , NaH 2 PO 4 etc. are used for potassium ions, KCl, KHCO 3 , KH 2 PO 4 etc. are used for chlorine ions, NaCl, KCl etc. are used.
実施例 １
血清中のNa+、K+およびCl-のイオン濃度を液
膜型イオン選択性電極を用いて測定し、測定の間
に、バチリス属のバクテリアの生産したアルカリ
性プロテアーゼ（比活性600000単位／ｇ、塩類含
有量50mg／ｇ）50単位／mlおよび水を含む洗浄液
で較正の前に電極を洗浄し、さらに、NaCl0.2
ｇ／、KH2PO40.02ｇ／、NaHCO30.1ｇ／
、トリスヒドロキシメチルアミノメタン4.85
ｇ／、ホウ酸4.02ｇ／および水よりなる内部
標準液で較正しつつ、血清を測定したところ、１
週間で約6000検体の測定を行なつたが、電極の汚
染はなく、正常に機能した。Example 1 The ion concentrations of Na + , K + and Cl - in serum were measured using a liquid film type ion-selective electrode. Clean the electrode before calibration with a cleaning solution containing 50 units/ml (salt content 50 mg/g) and water;
g/, KH 2 PO 4 0.02g/, NaHCO 3 0.1g/
, trishydroxymethylaminomethane 4.85
When serum was measured while calibrating with an internal standard solution consisting of boric acid 4.02 g/g/g/, boric acid 4.02 g/g/g and water, it was found that 1
Approximately 6,000 samples were measured in a week, and the electrodes were not contaminated and functioned normally.
比較のため上記洗浄液にプロテアーゼを含有さ
せないで行なつた場合（ただし、電極の感応膜部
分の機械的洗浄など全く洗浄操作を行なわないも
のとする。）、測定をはじめて、約500検体（１日
目）を測定したのちは、塩素イオンの測定ができ
なくなつた。このときのCl-電極の起電力は正常
値の70％以下に低下していた。 For comparison, when the above washing solution was carried out without containing protease (however, no cleaning operations such as mechanical cleaning of the sensitive membrane part of the electrode were performed), approximately 500 samples (1 day) were measured after the first measurement. After measuring chlorine ions, it was no longer possible to measure chloride ions. At this time, the electromotive force of the Cl - electrode had decreased to 70% or less of its normal value.
以上より明らかなように、プロテアーゼを含有
する洗浄液を使用することにより、電極に付着し
た蛋白質を効率よく除去することができ、生体成
分中の物質の測定を円滑に行なうことができる。 As is clear from the above, by using a cleaning solution containing protease, proteins adhering to the electrode can be efficiently removed, and substances in biological components can be measured smoothly.
Claims (1)
膜電極の洗浄液であつて、水およびプロテアーゼ
を含有してなる洗浄液。 ２ 生体成分が血液、血清または尿である特許請
求の範囲第１項記載の洗浄液。 ３ プロテアーゼがアルカリ性プロテアーゼであ
る特許請求の範囲第１項または第２項記載の洗浄
液。 ４ プロテアーゼがバチルス属のバクテリアによ
つて生産されたアルカリ性プロテアーゼである特
許請求の範囲第１項、第２項または第３項記載の
洗浄液。[Scope of Claims] 1. A cleaning liquid for a polymer membrane electrode for measuring substances in biological components, the cleaning liquid containing water and protease. 2. The cleaning liquid according to claim 1, wherein the biological component is blood, serum, or urine. 3. The cleaning solution according to claim 1 or 2, wherein the protease is alkaline protease. 4. The cleaning solution according to claim 1, 2 or 3, wherein the protease is an alkaline protease produced by a bacterium belonging to the genus Bacillus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14462380A JPS5767856A (en) | 1980-10-15 | 1980-10-15 | Washing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14462380A JPS5767856A (en) | 1980-10-15 | 1980-10-15 | Washing liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5767856A JPS5767856A (en) | 1982-04-24 |
| JPS632343B2 true JPS632343B2 (en) | 1988-01-18 |
Family
ID=15366335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14462380A Granted JPS5767856A (en) | 1980-10-15 | 1980-10-15 | Washing liquid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5767856A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0295377U (en) * | 1989-01-13 | 1990-07-30 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0134116Y2 (en) * | 1981-02-12 | 1989-10-17 | ||
| JPS58187847A (en) * | 1982-04-28 | 1983-11-02 | Toshiba Corp | Method for washing electrode apparatus |
| JPS58187849A (en) * | 1982-04-28 | 1983-11-02 | Toshiba Corp | Cleansing method for electrode device |
| JP2007255898A (en) * | 2006-03-20 | 2007-10-04 | Horiba Ltd | Washing agent for glass electrode, washing method of glass electrode, and package for glass electrode washing agent |
| CN107085026A (en) * | 2017-04-01 | 2017-08-22 | 合肥迪安医学检验所有限公司 | A kind of ion-selective electrode Precerving liquid |
| EP3842795A4 (en) * | 2018-08-28 | 2022-05-11 | HORIBA Advanced Techno, Co., Ltd. | Calcium ion concentration-measuring device |
-
1980
- 1980-10-15 JP JP14462380A patent/JPS5767856A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0295377U (en) * | 1989-01-13 | 1990-07-30 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5767856A (en) | 1982-04-24 |
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