JPS63207400A - Discoloration of molasses - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for treating waste honey so that it can be used in fields such as culture or fermentation.

For example, it can be used in the bakery yeast industry, alcohol industry, food industry, amino acid fermentation industry, and other fermentation industries.

[Conventional technology]

Since S molasses contains sugar, vitamins, minerals, etc., is inexpensive, and has a stable supply, it has generally been widely used as a raw material for fermentation media.

However, as these blackstrap molasses are heated at high temperatures during the sugar manufacturing process, Maillard reactions occur, and as a result, they contain melanoidin-based dark brown pigments, which are extremely colored. This pigment has often been effectively removed when using M honey as a fermentation medium. That-
For example, methods using adsorbents such as activated carbon, methods using hydrophilic organic solvents, methods using ultrafiltration membranes, etc. have been carried out.

[Problem that the invention seeks to solve]

Among the conventional techniques, methods using activated carbon or hydrophilic organic solvents are difficult to put into practical use because they require high costs for regenerating the activated carbon and recovering the solvent, and problems arise in the treatment of by-product industrial waste. Ta. Furthermore, in the method using an ultrafiltration membrane, the permeation rate of blackstrap molasses and its contents is low, and
In order to obtain a certain amount, a large membrane area is required, which leaves problems such as processing costs.

Therefore, waste 11 & honey has generally been used as a fermentation medium without satisfactory decolorization.

[Means for solving problems]

As a result of extensive research into membrane filtration as an economical method for decolorizing blackstrap molasses, the present inventors found that by using a specific filtration membrane, blackstrap molasses can be simply diluted with water to an appropriate concentration. We have discovered that it can be directly filtered and that it can effectively decolorize without losing the sugars and vitamins necessary for fermentation.

It was also confirmed that, for economic reasons, it is necessary to increase the filtration rate of waste honey to allow it to pass through the filtration membrane in a short period of time for industrialization. As a result of various studies for this purpose, we found that a method to increase the filtration rate without causing any harmful changes in the components necessary for fermentation contained in the blackstrap molasses was to ferment the blackstrap molasses on a regular basis. We have come to the conclusion that this is preferable.

From this point of view, as a result of extensive studies on enzymatic treatment, we found that blackstrap molasses treated with enzymes, especially invertase, was 1-lower than blackstrap molasses without enzyme treatment.
Not only did the permeation rate increase by 40%, but the obtained permeate was not adversely affected by fermentation components, an extremely useful new finding.

The present invention has been made based on these new findings, and is characterized in that blackstrap molasses and its contents treated with invertase are filtered using an ultrafiltration membrane or a reverse osmosis membrane. This is a bleaching method.

The filtration membranes used in the present invention include ultrafiltration membranes and reverse osmosis membranes. Preferably, the membrane has a sugar rejection rate of 0 to 30% and a decolorization rate of 40 to 90%.

The sugar rejection rate here is expressed by the following formula.

Further, the decolorization rate is similarly expressed by the following formula.

There are no particular limitations on the membrane material; polyvinyl alcohol, polysulfone, polyethersulfone, polyamide,
It is made of polyolefin, polyacrylonitrile, polycarbonate, polymethylmethacrylate-1-, cellulose, other known polymers, or mixtures thereof, and the membrane shape can be flat membrane, hollow fiber, spiral, tubular, etc. It's okay.

Filtration is generally carried out at a pressure of 1 to 50 kg/aJ. Further, the temperature is room temperature to 80°C.

Preferably, in order to increase the filtration rate, it is optimal to carry out the reaction at a temperature of 50 to 70°C.

Further, the sugar concentration can be appropriately determined in consideration of filtration speed and economic efficiency, but it is usually carried out at 20 to 50 BX (Brix), preferably 40 to 50 BX.

Further, there is no need to incorporate any particular sterilization process.

In the present invention, it is necessary to previously treat the blackstrap molasses with invertase. Although molasses is preferably adjusted to the sugar concentration described above, it can be used without any special treatment, which is also one of the important advantages of the present invention. Of course, conventionally known heated clarified molasses can be used.

Invertase of any origin can be used, and purified ones, those with a low degree of purification, or those containing invertase such as cultured products can also be freely used. p of blackstrap molasses to be treated depending on the invertase used
It is necessary to adjust H, and usually a range of 5 to 8 is preferred, such as Asp.

For those derived from S. oryzae, the number is preferably 6 to 8.

This invertase treatment decomposes sucrose in blackstrap molasses into glucose and fructose, resulting in
It seems pointless to further decompose sucrose, which is very small compared to the membrane's original molecular weight cutoff, which can increase the permeation rate of the membrane, but we were able to obtain an unexpected increase in the permeation rate. .

A possible reason for this is that sucrose decomposes and becomes even lower in molecular weight, making it easier to pass through the membrane.

The invertase concentration needs to be appropriately determined at the time of use depending on the type of molasses to be treated and the enzyme titer. For example, invertase derived from baker's yeast (2500-5000
units/mQ), it is preferable to use approximately 0.25 to 5 ts (about 1 liter) per gram of clarified molasses diluted to a sugar concentration of 30% and heated and clarified. After adding invertase in this way, 5
While maintaining the optimum temperature for invertase at 5° C., the mixture is allowed to stand or is stirred for 5 to 30 hours to convert the sucrose in the molasses.

The obtained invertase-treated blackstrap molasses can be directly filtered through the above-mentioned filter membrane, and the filtration rate is about 20 to 40% faster than that of blackstrap molasses that is not subjected to enzyme treatment, such as conventional heat-clarified molasses, and the processing is speeded up.

〔Example〕

Heated clarified molasses diluted to 30% sugar concentration (BX42) 20
Add 25 d of invertase solution (derived from baker's yeast cell wall, titer 3800 units/mQ) to Ω,
Conversion treatment was carried out at 48° C. and 1100 rpm for 5 hours to prepare inverted molasses. Table 1 shows the analysis results of sugar before and after conversion treatment.

Table 1: Sugar analysis results heated and clarified! S Honey Converted M Honey Raffinose 0.485 0°267
Sew Claus 16゜4 0.256
Glucose 5.90 14.9 Fructose 6゜73 17.6 units [
g/100mQ) Table 2 Membrane performance Manufacturer Sumitomo Bakelite name TP-2011 Material Polysulfone shape External pressure tubular cut off molecular weight 10000 (dextran TIO 10%
(listening) Next, using a filter having an external pressure tubular membrane module with the performance shown in Table 2, the operating pressure was 1°92MPa and the temperature was 55°C.
The above-mentioned inverted molasses was filtered under the conditions of a linear velocity of 1.4 ie/see. At the same time, for comparison, filtration was carried out under the same conditions using heated clarified molasses that had not been subjected to conversion treatment, and the results are shown in Table 3 and Figure 1.

Table 3 Membrane filtration results Method according to the present invention Conventional method Decolorization rate c%) 90.0
g6.0 Sugar inhibition rate [%] 3.0
11.1 Transmission rate [Q/nEh]
8.0 5.5 Two types of permeate obtained by the above operations.

Baker's yeast was cultured using heated clarified molasses that was not subjected to membrane treatment. The results are shown in Table 4.

Table 4 Yeast culture results and membrane treatment yield [%] 137 1.38 138 Bacterial cell analysis carbohydrate [%] 35 36
36 Nitrogen (%) 7.7 6.1
5.8 phosphorus [%] 1.3
1.6 1.5
Hunter Ippakudo (-) 32 38
39 From these study results, in terms of decolorization using a membrane, compared to conventional heated clarified molasses that does not undergo enzymatic conversion treatment,
It was possible to significantly improve the transmission rate without changing the decolorization rate. In terms of performance, the obtained permeate has
In the culture of baker's yeast, results equivalent to those of conventional permeate were obtained, and it became clear that the enzymatic conversion treatment had no adverse effects.

〔Effect of the invention〕

According to the method of the present invention, since blackstrap molasses is treated with invertase, the filtration rate is increased, and BX4
Decolorization was performed efficiently even at high sugar concentrations of 0 to 50.

Furthermore, treatment with invertase has no adverse effects on the performance of decolorized blackstrap molasses. Rather, new uses can be considered as a fermentation raw material for microorganisms that cannot assimilate sucrose.

In order to treat waste molasses, which has high viscosity and many impurities, with a membrane, the permeation rate largely controls the economic efficiency.

That is, since the permeation rate is originally low, industrial use requires a large membrane area and considerable equipment costs. Furthermore,
The life of the membrane is short due to severe contamination of the membrane surface by impurities.
The running cost 1- required for membrane replacement increases. Therefore, in order to improve economic efficiency, it is necessary to reduce as much as possible the total membrane area required to filter a predetermined amount of molasses. For this purpose, the most important factor is how to increase the permeation rate per unit area, and the industrial significance of increasing the permeation rate by 20 to 40% by invertase treatment in the present invention is extremely large.

[Brief explanation of the drawing]

The figure illustrates the change in permeation rate between invertase-treated heated clarified molasses and conventional heated clarified molasses.

Claims (2)

[Claims] (1) A method for decolorizing blackstrap molasses, which comprises filtering blackstrap molasses and its contents treated with invertase using an ultrafiltration membrane or a reverse osmosis membrane. (2) The method for decolorizing blackstrap molasses according to claim 1, characterized in that a membrane having a sugar rejection rate of 0 to 30% and a decolorization rate of 40 to 80% is used.