JPS63179811A - Skin cosmetic - Google Patents
Skin cosmeticInfo
- Publication number
- JPS63179811A JPS63179811A JP1091887A JP1091887A JPS63179811A JP S63179811 A JPS63179811 A JP S63179811A JP 1091887 A JP1091887 A JP 1091887A JP 1091887 A JP1091887 A JP 1091887A JP S63179811 A JPS63179811 A JP S63179811A
- Authority
- JP
- Japan
- Prior art keywords
- skin
- cosmetic
- polygalactosamine
- test
- galactosamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002537 cosmetic Substances 0.000 title claims abstract description 21
- 150000003839 salts Chemical class 0.000 claims abstract description 13
- MSWZFWKMSRAUBD-GASJEMHNSA-N 2-amino-2-deoxy-D-galactopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O MSWZFWKMSRAUBD-GASJEMHNSA-N 0.000 claims abstract description 11
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 claims abstract description 11
- 150000004676 glycans Chemical class 0.000 claims abstract description 9
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 9
- 239000005017 polysaccharide Substances 0.000 claims abstract description 9
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 25
- 239000000203 mixture Substances 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 102000011782 Keratins Human genes 0.000 abstract description 5
- 108010076876 Keratins Proteins 0.000 abstract description 5
- 239000006210 lotion Substances 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 4
- 239000000470 constituent Substances 0.000 abstract description 3
- 239000006071 cream Substances 0.000 abstract description 2
- 239000003906 humectant Substances 0.000 abstract description 2
- 239000002932 luster Substances 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 1
- 206010040849 Skin fissures Diseases 0.000 abstract 1
- 239000000839 emulsion Substances 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 57
- 238000012360 testing method Methods 0.000 description 24
- 235000002639 sodium chloride Nutrition 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000010998 test method Methods 0.000 description 7
- 230000003020 moisturizing effect Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000014759 maintenance of location Effects 0.000 description 5
- 239000002884 skin cream Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000001953 sensory effect Effects 0.000 description 4
- 210000000434 stratum corneum Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000004909 Moisturizer Substances 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000001333 moisturizer Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 210000000736 corneocyte Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 229910000077 silane Inorganic materials 0.000 description 2
- 230000036620 skin dryness Effects 0.000 description 2
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- ODHCTXKNWHHXJC-VKHMYHEASA-M 5-oxo-L-prolinate Chemical compound [O-]C(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-M 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 206010039792 Seborrhoea Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 239000010696 ester oil Substances 0.000 description 1
- 238000004299 exfoliation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000037312 oily skin Effects 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 235000019271 petrolatum Nutrition 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940071139 pyrrolidone carboxylate Drugs 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229940032094 squalane Drugs 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000012134 supernatant fraction Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
Description
【発明の詳細な説明】
(発明の分野)
本発明はガラクトサミンを主構成成分とする多糖及び/
またはその塩を配合してなる、皮膚の水分保持機能(荒
肌改善効果、角質改善効果、保湿効果)を改善し、美肌
効果を呈する皮膚化粧料に関する。DETAILED DESCRIPTION OF THE INVENTION (Field of the Invention) The present invention relates to a polysaccharide containing galactosamine as a main component and/or
The present invention relates to a skin cosmetic containing a salt thereof, which improves the moisture retention function of the skin (improving rough skin, improving keratin, moisturizing effect) and exhibits a beautifying effect.
(従来技術)
従来より、健常な美しい皮膚を保持する為に、皮膚に適
度な水分と油分を与える親水性の皮膚保湿剤と油性の皮
膚柔軟剤を皮膚化粧料に配合することが行われている。(Prior art) In order to maintain healthy and beautiful skin, hydrophilic skin moisturizers and oily skin softeners that provide appropriate moisture and oil to the skin have traditionally been blended into skin cosmetics. There is.
皮膚保湿剤には、グリセリン、プロピレングリコール、
ポリエチレングリコール、ピロリドンカルボン酸塩等が
利用されているが、こしらは、皮膚の最外層である角質
層の水分を吸水して、かえって皮膚の水分を損失する原
因となることがあり、また、多量に含有する皮膚化粧料
にあっては、べたつくなどの違和感を与えるなど、必ず
しも満足出来るものではなかった。Skin moisturizers include glycerin, propylene glycol,
Polyethylene glycol, pyrrolidone carboxylate, etc. are used, but these can absorb moisture from the stratum corneum, the outermost layer of the skin, and cause moisture loss in the skin. Skin cosmetics that contain a large amount of these substances are not always satisfactory, as they give an uncomfortable feeling such as stickiness.
また、皮膚柔軟剤には、流動パラフィン、ワセリン、オ
リーブ油、スクアラン、ラノリン、合成エステル油等が
利用されているが、こnらも、表皮よりの水分蒸散を充
分に防ぐ程度に皮膚化粧料゛に含有せしめるときには、
皮膚の正常なる新陳代謝を阻害する原因となるなどの欠
点を有していた。In addition, liquid paraffin, petrolatum, olive oil, squalane, lanolin, synthetic ester oil, etc. are used as skin softeners; When it is contained in
It has drawbacks such as inhibiting the normal metabolism of the skin.
(発明の開示)
本発明者等は、皮膚保湿剤、皮膚柔軟剤にみられる上記
の欠点に鑑み、皮膚を健常な状態に保持しつつ、皮膚の
水分保持機能を持続的に改善するような皮膚化粧料を提
供することを目的として鋭意研究した結果、ガラクトサ
ミンを主構成成分とする多糖及び/またはその塩を配合
してなる皮膚化粧料が該目的に合致する効果を発現し、
更には、皮膚に湿潤性(しっとり惑)、柔軟性(滑らか
惑ン、弾力性及び艶を与える美肌効果を有することを見
出して本発明を完成する(こ至また。(Disclosure of the Invention) In view of the above-mentioned drawbacks of skin moisturizers and emollients, the present inventors have developed a method that maintains the skin in a healthy state and continuously improves the moisture retention function of the skin. As a result of intensive research aimed at providing skin cosmetics, it was discovered that a skin cosmetic containing polysaccharide and/or its salt containing galactosamine as a main component exhibits an effect that meets the purpose.
Furthermore, the present invention was completed by discovering that the skin has a beautifying effect by imparting moisture, softness, elasticity, and luster to the skin.
(発明の目的〕
本発明の目的は、皮膚の水分保持機能(荒肌改善効果、
角質改善効果、保湿効果)と美肌効果(官能テスト)等
の優れた皮膚化粧料を提供するにある。(Objective of the Invention) The object of the present invention is to provide moisture retention function of the skin (improving effect on rough skin,
Our goal is to provide skin cosmetics with excellent keratin-improving effect, moisturizing effect) and skin-beautifying effect (sensory test).
(発明の構成)
本発明はガラクトサミンを主構成成分とする多糖及び/
またはその塩を配合してなる皮膚化粧料である。(Structure of the Invention) The present invention provides a polysaccharide containing galactosamine as a main component and/or
Or a skin cosmetic containing the salt thereof.
(191成の具体的な説明)
本発明に用いるガラクトサミンを主構成成分とする多糖
(以下、ポリガラクトサミンと略記する)は、公知の物
質であって、特公昭56−12689号公報及びアグリ
カルチュラル・アンド・バイオロジカル・ケミストリー
(Agricultural andBiologi
oal Ohemistry )、第49巻、第815
9〜8164頁、1985年、並びにザ・ジャーナル・
オブ・バイオロジカル・ケミストリー(ThθJour
nal of Biological Ohemist
ry)、第285巻、第2588〜2541頁、196
0年、9月等にはその製造法と化学特性が記載されてい
る。(Specific explanation of 191 composition) The polysaccharide containing galactosamine as a main component (hereinafter abbreviated as polygalactosamine) used in the present invention is a known substance, and is disclosed in Japanese Patent Publication No. 12689/1989 and Agricultural and Biological Chemistry
oal chemistry), Volume 49, No. 815
9-8164, 1985, and The Journal
of Biological Chemistry (ThθJour)
nal of Biological Ohemist
ry), Volume 285, Pages 2588-2541, 196
The manufacturing method and chemical properties are described in Year 0, September, etc.
例えば、上記特公昭56−12689号公報の記載(こ
準じる製造法にて、後記の製造例1の如く、ペエシロマ
イセス属(Paecilomyces )に属するポリ
ガラクトサミン生産菌(微工研寄託FERM−PN0.
8928)の培養物より高純度のポリガラクトサミン(
ポリガラクトサミンA)を得た。For example, as described in the above-mentioned Japanese Patent Publication No. 56-12689 (by a production method similar to this), as in Production Example 1 below, a polygalactosamine-producing bacterium belonging to the genus Paecilomyces (FERM-PN0.
Polygalactosamine (8928) with higher purity than the culture of
Polygalactosamine A) was obtained.
本発明に用いるポリガラクトサミンは、構成アミノ糖と
してガラクトサミンを90モル%以上含有することが好
ましく、一部にN−アセチル化物等を含有するものであ
ってもよい。The polygalactosamine used in the present invention preferably contains 90 mol% or more of galactosamine as a constituent amino sugar, and may partially contain N-acetylated products.
これらのポリガラクトサミンは、後述の緒特性に於いて
、すべて同様な効果を呈することが確認された。It was confirmed that all of these polygalactosamines exhibit similar effects in terms of the properties described below.
また、ポリガラクトサミンは水に難等性であり、必要に
応じて、水浴解性を高めるために、ポリガラクトサミン
と通常の酸との中和塩の形で用いられる。かかる塩とし
ては、塩酸塩、酢酸塩、乳酸塩、燐酸塩等が好適である
。Furthermore, polygalactosamine is not resistant to water, and if necessary, it is used in the form of a neutralized salt of polygalactosamine and a normal acid in order to improve water bath dissolution. Suitable examples of such salts include hydrochloride, acetate, lactate, and phosphate.
製造例1
(1) ポリガラクトサミンAの製造■ グルコース
600F、ポリペプトン60f。Production Example 1 (1) Production of Polygalactosamine A■ Glucose 600F, Polypeptone 60F.
0a012・2H20125fを水道水174に溶解し
、濃NaOH溶液でPH7,0iこ調整した後807容
ジャーファーメンタ−に移し、この培地浴液に蒸気を注
入することにより加圧、加熱滅菌(121℃、20分間
)を行った。0a012.2H20125f was dissolved in 174 ml of tap water, adjusted to pH 7.0 with concentrated NaOH solution, transferred to an 807 ml jar fermenter, and sterilized under pressure and heat (121°C) by injecting steam into the culture medium bath. , 20 minutes).
■ 冷却後の培地(最終液量207)に、500m1容
三角フラスコ中、t5gmIlの同組成の培地(グルコ
ース8%、ポリペプトン0.8%、Oa(M2O,5%
、PH7,0)を用いて26“Cで4日間振盪培養した
ペエシロマイセスニー1(FIRM−PNo、8928
)を、容量比で約10%無菌的に接種した後、温度27
℃、通気fi 5 VVM、撹拌数20 ORPM 1
7)条件で5日間培養した。■ To the medium after cooling (final liquid volume 207), in a 500 ml Erlenmeyer flask, add a medium with the same composition of t5gml (glucose 8%, polypeptone 0.8%, Oa (M2O, 5%).
, PH7,0) and cultured with shaking at 26"C for 4 days.
) was aseptically inoculated at a volume ratio of approximately 10%, and then the temperature was 27°C.
°C, ventilation fi 5 VVM, stirring number 20 ORPM 1
7) The cells were cultured for 5 days under the following conditions.
■ 培養終了後培養物をP布P遇することにより培養P
液171を得た。■ After culturing, the culture material is placed on P cloth.
Liquid 171 was obtained.
■ この培養P液を50°C〜60°Cに加熱しながら
分画分子量16万の限外濾過膜(三菱レイヨン・エンジ
ニアリング社製UF膜チューブラ−モジュールFタイプ
)を通過させることにより、低分子画分を除き液量が約
84になる迄濃縮した。■ By passing this culture P solution through an ultrafiltration membrane (UF membrane tubular module F type manufactured by Mitsubishi Rayon Engineering Co., Ltd.) with a molecular weight cutoff of 160,000 while heating it to 50°C to 60°C, low molecular weight The fractions were removed and concentrated until the liquid volume was approximately 84.
■ 更に、約14,000XGで遠心分離することによ
り菌体残査、熱変性蛋白質を除去した。(2) Furthermore, bacterial cell residue and heat-denatured proteins were removed by centrifugation at approximately 14,000×G.
■ 遠心分離後の上澄液画分81に食塩約600f(約
20%濃度)を加え撹拌し、俗解後、濃NaOHでpa
を7.0〜8.5に調整した。−夜装置し塩析物を十分
析出させた後、サラン製の布(塩化ビニリデンと塩化ビ
ニールの共重合体)上に塩析物を回収し、更に、この塩
折物の上から大量の微アルカリ性の水(PH7,0以上
〕を撒布することにより、余分の食塩及び培養液に同時
に混在している中性糖、その他の夾雑物を洗い流した。■ Approximately 600f of common salt (approximately 20% concentration) was added to the supernatant fraction 81 after centrifugation, stirred, and then diluted with concentrated NaOH.
was adjusted to 7.0 to 8.5. -After setting up the apparatus at night and extracting 100% of the salted-out material, the salted-out material was collected on a Saran cloth (vinylidene chloride and vinyl chloride copolymer), and a large amount of By spraying slightly alkaline water (pH 7.0 or higher), excess salt, neutral sugars and other impurities mixed in the culture solution were washed away.
■ 次に、水洗後の塩析物に0.1 M塩酸溶液を容量
比で約8倍量加え溶解した。この溶解物に濃NaOH溶
液を加え、ポリガラクトサミンの等電点であるPH8,
5に合せた。−夜装置し十分析出物を析出させた後、上
記と同様サラン製の布上に析出物を回収し、大量の水道
水で洗った。この水洗物をもう一度0.1M塩酸に溶解
後、等電点沈澱を行い水洗を繰返すことにより精製した
。(2) Next, approximately 8 times the volume of 0.1 M hydrochloric acid solution was added to the salted out product after washing with water and dissolved. Concentrated NaOH solution was added to this solution, and pH 8, which is the isoelectric point of polygalactosamine, was
Adjusted to 5. - After the apparatus was used at night to precipitate the ten-analyte precipitate, the precipitate was collected on a saran cloth in the same manner as above and washed with a large amount of tap water. This water-washed product was dissolved once again in 0.1M hydrochloric acid, subjected to isoelectric precipitation, and purified by repeated washing with water.
■ この精製した塩析物を121℃、15分間滅菌後、
凍結乾燥すること1こより、ガラクトサミンを主成分と
する多糖の精製粉末(ポリガラクトサミン人、ガラクト
サミンとしての純度的96%)を7F得た。■ After sterilizing this purified salting out product at 121°C for 15 minutes,
By freeze-drying, purified polysaccharide powder (polygalactosamine, 96% purity as galactosamine) containing galactosamine as a main component was obtained as 7F.
■ また、用途により上記精製粉末の1部を0、1 M
塩酸に俗解し、分画分子j180万の限外濾過膜(アミ
コン社製分子篩膜タイプXM800)で分画し、ポリガ
ラクトサミンA1(分子量16万〜80万)とポリガラ
クトサミンA2(分子量80万以上〕に分画した。■ Depending on the purpose, one part of the above purified powder may be added to 0 or 1 M.
Commonly known as hydrochloric acid, it is fractionated using an ultrafiltration membrane with a molecular weight fraction of J1.8 million (Molecular Sieve Membrane Type XM800 manufactured by Amicon) to produce polygalactosamine A1 (molecular weight 160,000 to 800,000) and polygalactosamine A2 (molecular weight 800,000 or more). It was fractionated into
(2)精製ポリガラクトサミン人の特性■ 外観性状;
淡褐色粉末
■ 液性;水分散液PH=7.5
■ 呈色反応;、ニンヒドリン反応 十キサントプ
ロティン反応 −
エーリッヒ反応 −
モリッシュ反応 士
フェノール硫酸法 士
レローセントテスト −
■ 電気泳動;密度勾配等電点電気泳動により単一物質
として確認され、等電
点(pI)は8.5である。(2) Characteristics of purified polygalactosamine people ■ Appearance properties;
Light brown powder ■ Liquidity; aqueous dispersion PH = 7.5 ■ Color reaction; ninhydrin reaction Dexantoprotein reaction - Ehrlich reaction - Morisch reaction Phenol sulfuric acid method Lelowcent test - ■ Electrophoresis; Density gradient It was confirmed as a single substance by isoelectric focusing and has an isoelectric point (pI) of 8.5.
■ 元素分析;窒素8.64%、炭素42.80%、水
素6.87%、
一般式(C6H11NO4・X−H2O)n■ 分子量
;1部万以上
本発明に於いて、ポリガラクトサミン及びその塩は各々
単独で、或いは両者の混合物として配合すればよく、配
合量は、それらの合計量で皮膚化粧料(組成物)の総量
を基準として0.01〜5wt%の範囲が好適である。■ Elemental analysis: Nitrogen 8.64%, Carbon 42.80%, Hydrogen 6.87%, General formula (C6H11NO4・X-H2O) n ■ Molecular weight: 10,000 parts or more In the present invention, polygalactosamine and its salts They may be blended individually or as a mixture of the two, and their total blending amount is preferably in the range of 0.01 to 5 wt% based on the total amount of the skin cosmetic (composition).
配合量が0.01wt%未満では効果が充分に達成され
ず、一方5wt%を超えてもその増加分に見合った効果
の向上は望めない。If the amount is less than 0.01 wt%, the effect will not be sufficiently achieved, while if it exceeds 5 wt%, no improvement in effect commensurate with the increase can be expected.
本発明の皮膚化粧料に配合せる前記ポリガラクトサミン
及び/またはその塩は皮膚の表面で保湿性の高い被膜を
形成し、皮膚を健常な状態に保持しつつ、皮膚の水分保
持機能を持続的に改善するものと推察される。The polygalactosamine and/or its salt blended into the skin cosmetic of the present invention forms a highly moisturizing film on the skin surface, maintains the skin in a healthy state, and maintains the skin's moisture retention function. It is assumed that this will improve.
本発明の皮膚化粧料は、例えばローシ冒ン類、乳液類、
クリーム類、パック類等に適用することができる。The skin cosmetics of the present invention include, for example, lotions, milky lotions,
It can be applied to creams, packs, etc.
尚、本発明の皮膚化粧料には上記の他に色素、香料、防
腐剤、界面活性剤、保湿剤、抗酸化剤等を本発明の目的
を達成する範囲内で適宜配合することができる。In addition to the above, pigments, fragrances, preservatives, surfactants, humectants, antioxidants, and the like may be appropriately incorporated into the skin cosmetic of the present invention within a range that achieves the object of the present invention.
(実施例)
以下、実施例及び比較例1ζ基づいて本発明を詳説する
。(Example) Hereinafter, the present invention will be explained in detail based on Examples and Comparative Example 1ζ.
尚、保湿効果試験法、荒肌改善効果試験法、角質改善効
果試験法、官能テスト(美肌効果試験法)は下記の通り
である。The moisturizing effect test method, the rough skin improving effect test method, the keratin improving effect test method, and the sensory test (skin beautifying effect test method) are as follows.
(1) 荒肌改善効果試験法
下脚1ζ荒れ肌を有する中高年被験者20名を対象とし
て4週間連続塗布効果を調べた。被験者の左側下脚試験
部位に1日1回約1fの試料を塗布し、試験開始前およ
び終了後の皮膚の状態を下記の判定基準により判定した
。右側下脚は試料を塗布せず対照とした。(1) Test method for improvement of rough skin The effect of continuous application for 4 weeks was investigated on 20 middle-aged and elderly subjects with 1ζ rough skin on their lower legs. Approximately 1 f of the sample was applied to the test site of the left lower leg of the subject once a day, and the condition of the skin before and after the test was judged according to the following criteria. No sample was applied to the right lower leg, which served as a control.
皮膚乾燥度の判定基準
−二 正常
± : 軽微乾燥、落屑無し
十 二 乾燥、落屑軽度
十十: 乾燥、落屑中等度
十十十: 乾燥、落屑顕著
試験前後の試験部位と対照部位の判定結果を比較し、皮
膚乾燥度が2段階以上改善された場合(例えば+→−1
十十→±)を有効、1段階改善された場合をやや有効、
変化がなかった場合を無効とした。試験結果は有効、や
や有効となった被験者の人数で示しtこ。Judgment criteria for skin dryness - 2 Normal ±: Slight dryness, no flaking 12 Mild dryness, flaking 10: Moderate dryness and flaking 110: Significant dryness and flaking Judgment results of the test site and control site before and after the test If the skin dryness has improved by two or more levels (e.g. +→-1)
10→±) is valid, one level improvement is slightly valid,
If there was no change, it was considered invalid. The test results are shown in terms of the number of subjects who were found to be effective or somewhat effective.
(2)角質改善(角質細胞の抗剥離性増大)効果試験法
前述の荒n肌改善測定試験開始前および終了後の被験g
皮膚にスコッチテープにチバンメンディングテーブ)を
接着し、これを剥離した時テープに付着した角質細胞の
状態を走査型電子顕微鏡によって詳細に調べ、下記の基
準によって皮膚角質細胞抗剥離性を解析し、角質改善効
果を求めた。(2) Effect test method for improving keratin (increasing anti-exfoliation properties of keratinocytes) Test g before and after the rough skin improvement measurement test described above
Scotch tape (Tiban mending tape) was adhered to the skin, and when the tape was removed, the condition of the corneocytes attached to the tape was examined in detail using a scanning electron microscope, and the anti-removal properties of skin corneocytes were analyzed according to the following criteria. , the keratin-improving effect was sought.
角質改善効果の判定基型
評価点1 スケールを認めず
2 小スケール点在
8 小〜中スケール顕著
4 大スケール顕著
評価は4週間連続塗布後の試験部位の評価点と対照部位
のそれとの差が2点以上の場合を有効、1点の場合をや
や有効、0点の場合を無効とした。Judgment criteria for keratin improvement effect Evaluation points: 1 No scale observed 2 Small scales scattered 8 Small to medium scales noticeable 4 Large scale noticeable evaluation is based on the difference between the evaluation score of the test area and that of the control area after 4 weeks of continuous application. A score of 2 or more points was considered valid, a score of 1 point was considered somewhat effective, and a score of 0 points was considered invalid.
判定結果は有効、やや有効となった被験者の人数で示し
た。The judgment results are expressed as the number of subjects who found the test to be effective or somewhat effective.
(3) 保湿効果試験法
前記荒肌改善効果試験の開始前及び終了後、各被験者の
試験部位の皮膚コンダクタンス値(単位はマイクロモー
)を、インピーダンスメーター(工、B、8社製、IB
8−854型)を用いて測定した。(3) Moisturizing effect test method Before and after the start and end of the rough skin improvement effect test, the skin conductance value (unit: micromho) of the test site of each subject was measured using an impedance meter (manufactured by Kogyo, B, 8 companies, IB
8-854).
皮膚コンダクタンス値が大きい程一般に皮膚の電気抵抗
が小さく、皮膚の角質水分含有量が多いことが認められ
ている。It is generally accepted that the higher the skin conductance value, the lower the electrical resistance of the skin and the higher the stratum corneum moisture content of the skin.
保湿効果は、下記の式で求められる角質水分増加率(%
)より評価した。The moisturizing effect is determined by the rate of increase in stratum corneum moisture (%) calculated using the formula below.
) was evaluated.
Wo:試料塗布部位の試験開始前のコンダクタンス値W
: 〃 〃終了時 〃試験結果は被験
者20名の角質水分増加率の平均値で示した。Wo: Conductance value W of the sample application site before the start of the test
: 〃 〃 At the end 〃 The test results are shown as the average value of the stratum corneum moisture increase rate of 20 subjects.
(4)官能テスト(美肌効果試験)
荒れ肌、小じわ、乾燥肌等を訴える女子被験者(85〜
55才)20人に試料を1日2回(朝タ〕連続8ケ月塗
布して8ケ月後の効果を評価した。(4) Sensory test (skin beautification effect test) Female subjects (85 and up) who complain of rough skin, fine wrinkles, dry skin, etc.
The sample was applied to 20 people (55 years old) twice a day (in the morning) for 8 consecutive months, and the effects were evaluated after 8 months.
試験結果は、皮膚の湿潤性、平滑性、弾力性の各項目に
対して、皮膚に潤いが生じた、皮膚が滑らかになった、
皮膚(こ張りが生じたと回答した人数で示した。The test results showed that the skin was moisturized, the skin was smooth, and the skin was smooth.
Skin (indicated by the number of people who answered that their skin became stiff)
実施例1〜8、比較例1
〔二層型スキンローシラン〕
下記の組成のごとく、製造例で得たポリガラクトサミン
Aとその塩類を第1表に記載の通りに配合して各々のス
キンローシランを調製し、前記諸試験を実施した。Examples 1 to 8, Comparative Example 1 [Two-layered skin lotion silane] Each skin lotion was prepared by blending polygalactosamine A obtained in the production example and its salts as shown in Table 1, as shown in the composition below. A silane was prepared and the tests described above were conducted.
(2)調製法
03)成分中ポリガラクトサミンAは(4)成分中に、
またポリガラクトサミンAの塩類はり)成分中に各々配
合し、囚、(6)成分を各々均一に溶解した後、(4)
成分と(0成分を混合撹拌分散し、次いで容器に充填す
る。使用時には内容物を均一に振盪分散して使用する。(2) Preparation method 03) In the component, polygalactosamine A is in the component (4).
In addition, salts of polygalactosamine A are mixed into the ingredients (6) and (4) after uniformly dissolving each of the ingredients.
The ingredients (0) are mixed, stirred and dispersed, and then filled into a container. When used, the contents are uniformly shaken and dispersed.
(3)特性
各二層型スキンローシロンの諸試験を実施した結果を第
1表右欄に記載した。(3) Characteristics The results of various tests conducted on each two-layer skin losilon are listed in the right column of Table 1.
Wi1表に示すごとく、比較例1のスキンローシロンと
比較して、実施例1〜8の本発明のスキンローシロンは
水分保持機能及び官能テストにおいて優れた評価が認め
られた。As shown in Table Wi1, compared to the Skin Lo Silon of Comparative Example 1, the Skin Lo Silon of the present invention of Examples 1 to 8 was evaluated to be superior in moisture retention function and sensory test.
実施例4〜8、比較例2
〔スキンクリーム〕
実施例1と同様に、下記の組成にて各々のスキンクリー
ムを調製し、諸試験を実施した結果を第1表右欄に示し
た。Examples 4 to 8, Comparative Example 2 [Skin Cream] In the same manner as in Example 1, skin creams were prepared with the following compositions and various tests were conducted.The results are shown in the right column of Table 1.
(2) 1m製法
ノ)成分中ポリガラクトサミン人は(4)成分中に、ま
たポリガラクトサミンAの塩類は働成分中に各々配合し
、(支)成分及び(O成分を各々80″Cに加熱溶解し
た後、混合して、撹拌しつつ80℃迄冷却して各スキン
クリームを調製した。(2) In the 1m manufacturing method, the polygalactosamine A in the component is mixed in the component (4), and the salts of polygalactosamine A are mixed in the working component, and the (supporting) component and the (O component) are heated to 80"C. After dissolving, each skin cream was prepared by mixing and cooling to 80° C. while stirring.
(3)特性
第1表に示すごとく、本発明の皮膚化粧料である実施例
4〜8のスキンクリームは、比較例2のスキンクリーム
と比較して、諸試験において優れた特性が認められた。(3) Properties As shown in Table 1, the skin creams of Examples 4 to 8, which are skin cosmetics of the present invention, had superior properties in various tests compared to the skin cream of Comparative Example 2. .
(発明の効果)(Effect of the invention)
Claims (2)
たはその塩を配合してなる皮膚化粧料。(1) A skin cosmetic containing a polysaccharide whose main component is galactosamine and/or its salt.
ロマイセス属(Paecilomyces)に属するポ
リガラクトサミン生産菌の培養物より得られる特許請求
の範囲第1項に記載の皮膚化粧料。(2) The skin cosmetic according to claim 1, wherein the polysaccharide containing galactosamine as a main component is obtained from a culture of polygalactosamine-producing bacteria belonging to the genus Paecilomyces.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1091887A JPS63179811A (en) | 1987-01-19 | 1987-01-19 | Skin cosmetic |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1091887A JPS63179811A (en) | 1987-01-19 | 1987-01-19 | Skin cosmetic |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS63179811A true JPS63179811A (en) | 1988-07-23 |
Family
ID=11763630
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1091887A Pending JPS63179811A (en) | 1987-01-19 | 1987-01-19 | Skin cosmetic |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS63179811A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100340185B1 (en) * | 1998-09-07 | 2002-10-31 | 김철진 | Anti-wrinkle cosmetic composition containing PAECILOMYCES JAPONICA extract |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62209021A (en) * | 1986-03-08 | 1987-09-14 | Lion Corp | Preventive and remedy for pimple |
-
1987
- 1987-01-19 JP JP1091887A patent/JPS63179811A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62209021A (en) * | 1986-03-08 | 1987-09-14 | Lion Corp | Preventive and remedy for pimple |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100340185B1 (en) * | 1998-09-07 | 2002-10-31 | 김철진 | Anti-wrinkle cosmetic composition containing PAECILOMYCES JAPONICA extract |
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