JPS63179811A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic having improved water retaining function of skin and beautifying effects on skin, by blending a cosmetic with a polysaccharide comprising galactosamine as a main constituent component and/or a salt thereof. CONSTITUTION:A skin cosmetic blended with a polysaccharide comprising galactosamine obtained from a culture mixture of a fungus belonging to the genus Paecilomyces, capable of producing polygalactosamine as a main constituent component. Since the cosmetic has water retaining function of skin and beautifying effects on skin, the cosmetic can provide skin with improving effects on chapped skin, improving effects on keratin, humectant effects, flexibility, elasticity and luster. Lotions, emulsion, creams, packs, etc., may be applied as the form of cosmetic.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of the Invention) The present invention relates to a polysaccharide containing galactosamine as a main component and/or
The present invention relates to a skin cosmetic containing a salt thereof, which improves the moisture retention function of the skin (improving rough skin, improving keratin, moisturizing effect) and exhibits a beautifying effect.

(Prior art) In order to maintain healthy and beautiful skin, hydrophilic skin moisturizers and oily skin softeners that provide appropriate moisture and oil to the skin have traditionally been blended into skin cosmetics. There is.

Skin moisturizers include glycerin, propylene glycol,
Polyethylene glycol, pyrrolidone carboxylate, etc. are used, but these can absorb moisture from the stratum corneum, the outermost layer of the skin, and cause moisture loss in the skin. Skin cosmetics that contain a large amount of these substances are not always satisfactory, as they give an uncomfortable feeling such as stickiness.

In addition, liquid paraffin, petrolatum, olive oil, squalane, lanolin, synthetic ester oil, etc. are used as skin softeners; When it is contained in
It has drawbacks such as inhibiting the normal metabolism of the skin.

(Disclosure of the Invention) In view of the above-mentioned drawbacks of skin moisturizers and emollients, the present inventors have developed a method that maintains the skin in a healthy state and continuously improves the moisture retention function of the skin. As a result of intensive research aimed at providing skin cosmetics, it was discovered that a skin cosmetic containing polysaccharide and/or its salt containing galactosamine as a main component exhibits an effect that meets the purpose.
Furthermore, the present invention was completed by discovering that the skin has a beautifying effect by imparting moisture, softness, elasticity, and luster to the skin.

(Objective of the Invention) The object of the present invention is to provide moisture retention function of the skin (improving effect on rough skin,
Our goal is to provide skin cosmetics with excellent keratin-improving effect, moisturizing effect) and skin-beautifying effect (sensory test).

(Structure of the Invention) The present invention provides a polysaccharide containing galactosamine as a main component and/or
Or a skin cosmetic containing the salt thereof.

(Specific explanation of 191 composition) The polysaccharide containing galactosamine as a main component (hereinafter abbreviated as polygalactosamine) used in the present invention is a known substance, and is disclosed in Japanese Patent Publication No. 12689/1989 and Agricultural and Biological Chemistry
oal chemistry), Volume 49, No. 815
9-8164, 1985, and The Journal
of Biological Chemistry (ThθJour)
nal of Biological Ohemist
ry), Volume 285, Pages 2588-2541, 196
The manufacturing method and chemical properties are described in Year 0, September, etc.

For example, as described in the above-mentioned Japanese Patent Publication No. 56-12689 (by a production method similar to this), as in Production Example 1 below, a polygalactosamine-producing bacterium belonging to the genus Paecilomyces (FERM-PN0.
Polygalactosamine (8928) with higher purity than the culture of
Polygalactosamine A) was obtained.

The polygalactosamine used in the present invention preferably contains 90 mol% or more of galactosamine as a constituent amino sugar, and may partially contain N-acetylated products.

It was confirmed that all of these polygalactosamines exhibit similar effects in terms of the properties described below.

Furthermore, polygalactosamine is not resistant to water, and if necessary, it is used in the form of a neutralized salt of polygalactosamine and a normal acid in order to improve water bath dissolution. Suitable examples of such salts include hydrochloride, acetate, lactate, and phosphate.

Production Example 1 (1) Production of Polygalactosamine A■ Glucose 600F, Polypeptone 60F.

0a012.2H20125f was dissolved in 174 ml of tap water, adjusted to pH 7.0 with concentrated NaOH solution, transferred to an 807 ml jar fermenter, and sterilized under pressure and heat (121°C) by injecting steam into the culture medium bath. , 20 minutes).

■ To the medium after cooling (final liquid volume 207), in a 500 ml Erlenmeyer flask, add a medium with the same composition of t5gml (glucose 8%, polypeptone 0.8%, Oa (M2O, 5%).
, PH7,0) and cultured with shaking at 26"C for 4 days.
) was aseptically inoculated at a volume ratio of approximately 10%, and then the temperature was 27°C.
°C, ventilation fi 5 VVM, stirring number 20 ORPM 1
7) The cells were cultured for 5 days under the following conditions.

■ After culturing, the culture material is placed on P cloth.
Liquid 171 was obtained.

■ By passing this culture P solution through an ultrafiltration membrane (UF membrane tubular module F type manufactured by Mitsubishi Rayon Engineering Co., Ltd.) with a molecular weight cutoff of 160,000 while heating it to 50°C to 60°C, low molecular weight The fractions were removed and concentrated until the liquid volume was approximately 84.

(2) Furthermore, bacterial cell residue and heat-denatured proteins were removed by centrifugation at approximately 14,000×G.

■ Approximately 600f of common salt (approximately 20% concentration) was added to the supernatant fraction 81 after centrifugation, stirred, and then diluted with concentrated NaOH.
was adjusted to 7.0 to 8.5. -After setting up the apparatus at night and extracting 100% of the salted-out material, the salted-out material was collected on a Saran cloth (vinylidene chloride and vinyl chloride copolymer), and a large amount of By spraying slightly alkaline water (pH 7.0 or higher), excess salt, neutral sugars and other impurities mixed in the culture solution were washed away.

(2) Next, approximately 8 times the volume of 0.1 M hydrochloric acid solution was added to the salted out product after washing with water and dissolved. Concentrated NaOH solution was added to this solution, and pH 8, which is the isoelectric point of polygalactosamine, was
Adjusted to 5. - After the apparatus was used at night to precipitate the ten-analyte precipitate, the precipitate was collected on a saran cloth in the same manner as above and washed with a large amount of tap water. This water-washed product was dissolved once again in 0.1M hydrochloric acid, subjected to isoelectric precipitation, and purified by repeated washing with water.

■ After sterilizing this purified salting out product at 121°C for 15 minutes,
By freeze-drying, purified polysaccharide powder (polygalactosamine, 96% purity as galactosamine) containing galactosamine as a main component was obtained as 7F.

■ Depending on the purpose, one part of the above purified powder may be added to 0 or 1 M.
Commonly known as hydrochloric acid, it is fractionated using an ultrafiltration membrane with a molecular weight fraction of J1.8 million (Molecular Sieve Membrane Type XM800 manufactured by Amicon) to produce polygalactosamine A1 (molecular weight 160,000 to 800,000) and polygalactosamine A2 (molecular weight 800,000 or more). It was fractionated into

(2) Characteristics of purified polygalactosamine people ■ Appearance properties;
Light brown powder ■ Liquidity; aqueous dispersion PH = 7.5 ■ Color reaction; ninhydrin reaction Dexantoprotein reaction - Ehrlich reaction - Morisch reaction Phenol sulfuric acid method Lelowcent test - ■ Electrophoresis; Density gradient It was confirmed as a single substance by isoelectric focusing and has an isoelectric point (pI) of 8.5.

■ Elemental analysis: Nitrogen 8.64%, Carbon 42.80%, Hydrogen 6.87%, General formula (C6H11NO4・X-H2O) n ■ Molecular weight: 10,000 parts or more In the present invention, polygalactosamine and its salts They may be blended individually or as a mixture of the two, and their total blending amount is preferably in the range of 0.01 to 5 wt% based on the total amount of the skin cosmetic (composition).

If the amount is less than 0.01 wt%, the effect will not be sufficiently achieved, while if it exceeds 5 wt%, no improvement in effect commensurate with the increase can be expected.

The polygalactosamine and/or its salt blended into the skin cosmetic of the present invention forms a highly moisturizing film on the skin surface, maintains the skin in a healthy state, and maintains the skin's moisture retention function. It is assumed that this will improve.

The skin cosmetics of the present invention include, for example, lotions, milky lotions,
It can be applied to creams, packs, etc.

In addition to the above, pigments, fragrances, preservatives, surfactants, humectants, antioxidants, and the like may be appropriately incorporated into the skin cosmetic of the present invention within a range that achieves the object of the present invention.

(Example) Hereinafter, the present invention will be explained in detail based on Examples and Comparative Example 1ζ.

The moisturizing effect test method, the rough skin improving effect test method, the keratin improving effect test method, and the sensory test (skin beautifying effect test method) are as follows.

(1) Test method for improvement of rough skin The effect of continuous application for 4 weeks was investigated on 20 middle-aged and elderly subjects with 1ζ rough skin on their lower legs. Approximately 1 f of the sample was applied to the test site of the left lower leg of the subject once a day, and the condition of the skin before and after the test was judged according to the following criteria. No sample was applied to the right lower leg, which served as a control.

Judgment criteria for skin dryness - 2 Normal ±: Slight dryness, no flaking 12 Mild dryness, flaking 10: Moderate dryness and flaking 110: Significant dryness and flaking Judgment results of the test site and control site before and after the test If the skin dryness has improved by two or more levels (e.g. +→-1)
10→±) is valid, one level improvement is slightly valid,
If there was no change, it was considered invalid. The test results are shown in terms of the number of subjects who were found to be effective or somewhat effective.

(2) Effect test method for improving keratin (increasing anti-exfoliation properties of keratinocytes) Test g before and after the rough skin improvement measurement test described above
Scotch tape (Tiban mending tape) was adhered to the skin, and when the tape was removed, the condition of the corneocytes attached to the tape was examined in detail using a scanning electron microscope, and the anti-removal properties of skin corneocytes were analyzed according to the following criteria. , the keratin-improving effect was sought.

Judgment criteria for keratin improvement effect Evaluation points: 1 No scale observed 2 Small scales scattered 8 Small to medium scales noticeable 4 Large scale noticeable evaluation is based on the difference between the evaluation score of the test area and that of the control area after 4 weeks of continuous application. A score of 2 or more points was considered valid, a score of 1 point was considered somewhat effective, and a score of 0 points was considered invalid.

The judgment results are expressed as the number of subjects who found the test to be effective or somewhat effective.

(3) Moisturizing effect test method Before and after the start and end of the rough skin improvement effect test, the skin conductance value (unit: micromho) of the test site of each subject was measured using an impedance meter (manufactured by Kogyo, B, 8 companies, IB
8-854).

It is generally accepted that the higher the skin conductance value, the lower the electrical resistance of the skin and the higher the stratum corneum moisture content of the skin.

The moisturizing effect is determined by the rate of increase in stratum corneum moisture (%) calculated using the formula below.
) was evaluated.

Wo: Conductance value W of the sample application site before the start of the test
: 〃 〃 At the end 〃 The test results are shown as the average value of the stratum corneum moisture increase rate of 20 subjects.

(4) Sensory test (skin beautification effect test) Female subjects (85 and up) who complain of rough skin, fine wrinkles, dry skin, etc.
The sample was applied to 20 people (55 years old) twice a day (in the morning) for 8 consecutive months, and the effects were evaluated after 8 months.

The test results showed that the skin was moisturized, the skin was smooth, and the skin was smooth.
Skin (indicated by the number of people who answered that their skin became stiff)

Examples 1 to 8, Comparative Example 1 [Two-layered skin lotion silane] Each skin lotion was prepared by blending polygalactosamine A obtained in the production example and its salts as shown in Table 1, as shown in the composition below. A silane was prepared and the tests described above were conducted.

(2) Preparation method 03) In the component, polygalactosamine A is in the component (4).
In addition, salts of polygalactosamine A are mixed into the ingredients (6) and (4) after uniformly dissolving each of the ingredients.
The ingredients (0) are mixed, stirred and dispersed, and then filled into a container. When used, the contents are uniformly shaken and dispersed.

(3) Characteristics The results of various tests conducted on each two-layer skin losilon are listed in the right column of Table 1.

As shown in Table Wi1, compared to the Skin Lo Silon of Comparative Example 1, the Skin Lo Silon of the present invention of Examples 1 to 8 was evaluated to be superior in moisture retention function and sensory test.

Examples 4 to 8, Comparative Example 2 [Skin Cream] In the same manner as in Example 1, skin creams were prepared with the following compositions and various tests were conducted.The results are shown in the right column of Table 1.

(2) In the 1m manufacturing method, the polygalactosamine A in the component is mixed in the component (4), and the salts of polygalactosamine A are mixed in the working component, and the (supporting) component and the (O component) are heated to 80"C. After dissolving, each skin cream was prepared by mixing and cooling to 80° C. while stirring.

(3) Properties As shown in Table 1, the skin creams of Examples 4 to 8, which are skin cosmetics of the present invention, had superior properties in various tests compared to the skin cream of Comparative Example 2. .

(Effect of the invention)

Claims (2)

[Claims] (1) A skin cosmetic containing a polysaccharide whose main component is galactosamine and/or its salt. (2) The skin cosmetic according to claim 1, wherein the polysaccharide containing galactosamine as a main component is obtained from a culture of polygalactosamine-producing bacteria belonging to the genus Paecilomyces.