JPS63159764A - Liquid dispensing and sucking device - Google Patents

Abstract

PURPOSE:To enable accurate quantitative water injection and to facilitate operation and maintenance by providing plural multiple nozzles vertically below a tank which is communicated with a feed water pump and a suction pump and has upper and lower layered chambers. CONSTITUTION:A rack where test-tubes are mounted slanting backward is placed on a mount table and multiple nozzles 5 drooping from the tank 6 are positioned right above the respective test-tubes. A tank 6 provided for each array of test-tubes has an upper chamber 7 for water injection and a lower chamber 8 for water suction across a partition wall. Nozzles 5 are double nozzles, the inside suction nozzle 9 penetrates the lower chamber 7, and its upper opening 10 faces the chamber 8. An injection nozzle 11 is arranged concentrically with the suction nozzle 9 while a gap is left at the periphery. Then a reagent and water are charged in the chamber 7 and injection nozzle 11 linked with the feed water pump by the feed water pump and the mount table for the test-tubes is elevated; when the lower opening of the suction nozzle 9 reaches a specific position, the elevation is stopped and the feed water pump is driven in the state for a certain time to inject a constant amount of water into the test-tubes.

Description

[Detailed Description of the Invention] [Field of Industrial Application] The present invention provides a method for placing a fixed amount of reagent into multiple test tubes containing different samples at once and aspirating unnecessary liquid after the reaction is completed.
Alternatively, the present invention relates to a liquid dispensing and absorbing device used when, for example, cleaning the inside of a test tube by sucking up a fixed amount of dilution water into a test tube.

[Conventional technology and problems]

Conventional equipment of this kind consists of a lifting platform on which multiple test tubes loaded in a rack are placed, and a panel etc. installed at intervals above the platform, through which water injection nozzles and water absorption nozzles are fixedly attached. A pipe attached to each nozzle connects them all together to the conduit of the water pump, and a separate pipe is similarly attached to each of the water suction nozzles, which are then collectively attached to the conduit of the water suction pump. Therefore, not only does this require an extremely large number of pipes equal to the number of water suction nozzles and water injection nozzles, making the device complicated, but also because the distance between the pump conduit and the nozzle is not constant, the length of the pipe varies depending on the position of the nozzle. As a result, there is a difference in the resistance within the flow path, especially in the water injection system, and even if the water supply pressure and water supply time are constant, more water will flow from the flow path with less resistance, and as a result, all the water will flow through the flow path with less resistance. There was a problem in that it was not possible to supply uniform and constant water. In addition, the nozzles in conventional devices are arranged side by side, and when used for a long time, samples in test tubes, reagent 1 reactants, etc. stick to the recesses of the joints, making it difficult to remove them. Not only that, but there was a risk that the reaction itself would be inhibited by the remaining deposits.

This device is also applied in quantitative analysis using isotopes as tracers, but this method is generally applied in serum tests, in which serum collected from different individuals is placed in test tubes, and the appropriate reagents and isotope are added to the test tube. This method is becoming popular as a method for determining the amount of the target substance in serum by inserting a sphere coated with chlorine and causing an antigen-antibody reaction on the sphere, and then measuring the change in the amount of radioactivity again. The important thing with this method is to remove all post-reaction serum and test without touching the spheres.

However, it is actually difficult to completely remove them, and quantitative measurements are actually performed by keeping the dilution rate constant. Therefore, when cleaning with this method, it is essential that a certain amount of diluent is injected and all of it is sucked out.

However, as described above, in the conventional apparatus, the amount of diluent varies depending on the position of the water injection nozzle, and residual liquid also adheres to the nozzle, making it impossible to perform accurate quantitative measurements. Furthermore, since the water injection nozzle and the water pump are connected through a large number of pipes, the device becomes complicated and maintenance such as cleaning and repair cannot be performed easily.

(Means for Solving the Problems) The present invention is aimed at solving the above-mentioned drawbacks, and has a method of injecting a precise amount of water into a test tube, sucking and discharging the water, and passing it through a nozzle. without leaving any residue
Furthermore, the aim is to provide a device that is compact overall and suitable for maintenance, and its gist is to have a multi-layered tank with a chamber communicating with the water pump and a chamber communicating with the water suction pump vertically. Moreover, the liquid dispensing and water absorption device is characterized in that a plurality of multiple nozzles are vertically installed below the tank, and each nozzle of the multiple nozzles communicates independently with each of the above-mentioned chambers.

One of the features of the present invention is that it has a tank in which the chambers are stacked vertically in multiple layers, that is, stacked in multiple stages. The tank may be made of stainless steel, but is usually made of chemically resistant hard plastic. One of the chambers is connected to the water pump through one pipe, and one of the other chambers is also connected to the water pump through one pipe.
It is connected to the water suction pump through a pipe. These chambers are stacked one above the other within the tank, and of course each chamber is individually separated. The simplest configuration is to divide the inside of the tank into two, upper and lower, with a lower chamber connected to the water pump and an upper chamber connected to the water suction pump.

If necessary, the number of chambers is two or more, and each chamber is communicated with a water pump or a water suction pump.

Note that the tank referred to here essentially has a chamber underneath which multiple nozzles can be installed; for example, the ends of two wooden pipes are stacked one above the other with an appropriate width. even if the end is included in the tank referred to herein.

Furthermore, the bottom of the chamber communicating with the water pump should be sloped gradually downward so that water can easily flow down toward the pipe.On the other hand, the chamber communicating with the water pump should have its top sloped downward. It is preferable to provide a concave area below which widens toward the end, and in the center of the concave area, nozzles to be described later are arranged one above the other.Furthermore, the above-mentioned tank is provided with a plurality of multiple nozzles under it, for example, Even if the nozzle is large enough to have many nozzles arranged in the vertical and horizontal directions, for example, it may be so large that five nozzles are arranged in one row, and the nozzles may be arranged in multiple rows.

Further, each chamber has a connection port with a pipe formed at the rear thereof, but it is preferable that the pipe be fixedly arranged and the tank removably installed.

Another feature of the invention is that multiple nozzles are arranged below such a tank, each of which communicates independently with each chamber of the tank.

Here, the term "multiple nozzles" refers to a nozzle consisting of one nozzle and nozzles arranged concentrically outside the nozzle,
The number is multiplexed according to the number of upper chambers. If there are only two chambers in the tank, it will be a double nozzle.

Each nozzle communicates separately with one of the chambers of the tank, with the inner nozzle facing its opening into the upper chamber and the outer nozzle facing its opening into its lower chamber. Let it happen. Thus, the inner nozzle, which is often a suction nozzle, penetrates the chamber below the chamber with which it communicates.

The outer nozzle is often a water injection nozzle, but it is desirable to arrange it concentrically with the water absorption nozzle. In order to support it in this way, the upper end of the nozzle must be partially buried in the ceiling of the room, that is, the partition wall. is desirable, and in this case, a slit, window, etc. is provided near the upper edge of the nozzle for communication.

The inner water absorption nozzle is the longest so that it reaches the bottom of the test tube, but the water supply nozzle does not need to be that long, and its lower end is positioned slightly above the lower end of the water absorption nozzle. disconnected.

Also at this time, a spacer provided with a slit is used to arrange the outer nozzle concentrically with respect to the inner nozzle.

Each of the multiple nozzles is positioned directly above a plurality of test tubes loaded in a rack below. Test tubes are arranged in rows and columns in racks to be processed once, but this equipment has a tank with a capacity that can supply the water required for all the test tubes, and the same number of nozzles as the test tubes below. Even if they are installed vertically in one tank.

A plurality of tanks having a capacity capable of supplying the necessary water for each row of test tubes may be provided in a plurality of rows, and multiple nozzles corresponding to the number of test tubes in the row below may be attached. In either case, it is preferable to make it removable. Note that it is desirable that the multiple nozzles be moved in and out of the test tubes by fixing the tank and raising and lowering the stand on which the test tube rack is placed.

[Effect]

Since the present invention is configured as described above, the chamber and the water injection nozzle which are connected to the chamber and the water injection nozzle are filled in advance with reagents and water via a pipe by a water pump, and the test tubes loaded in the rack are placed at a stand. When the lower opening of the water suction nozzle reaches near the bottom of the test tube, the rise is stopped, and in this state, the water pump is driven for a certain period of time. The supplied water passes through the pipe chamber and the water injection nozzle and is injected into the test tube, and the amount of water injected into each test tube is determined by the total amount of water supplied and the number of water injection nozzles communicating with that chamber.

As a result, a fixed amount of water is supplied to the test tube, and then the water suction pump is driven for a certain period of time. As a result, water is absorbed from the water absorption nozzle opened near the bottom of the test tube, and is discharged to the outside through the communicating chamber and pipe.

By repeating this several times, supply of a fixed amount of reagent or diluent and removal of unnecessary liquid, that is, dispensing and absorbing water, are completed.

〔Example〕

1 is a liquid dispensing and absorbing device; 2 is a stand on which a rack 4 loaded with test tubes 3 is placed; the test tubes 3 are tilted slightly backwards; Although it is only shown, several rows are arranged in the same way in the depth direction of the drawing. Reference numeral 5 designates a multi-nozzle whose tip is positioned directly above each of the illustrated test tubes 3, and is vertically installed below the tank 6.

The tank 6 provided for each row of five test tubes 3 has two upper and lower chambers separated by a partition wall, and a first chamber 7.
is a chamber for water injection, and the second chamber 8 is a chamber for water absorption.

Nozzle 5 is a double nozzle, and the inner nozzle is
The water absorption nozzle 9 penetrates the lower first chamber 7 and enters the second chamber 8.
An opening 10 is faced above it. Reference numeral 11 denotes a water injection nozzle arranged concentrically with a gap provided around the water absorption nozzle 9.

The area around the top plate 12 of the first chamber 7 where the nozzle 5 is arranged is as follows:
A recess 13 with a chevron-shaped cross section that widens toward the bottom is formed, and the upper end of the water injection nozzle 11 is embedded in the center, but a slit 14 formed at the edge of the opening faces into the chevron-shaped recess 13. The first chamber 7 and the water injection nozzle 11 are placed in communication with each other. In FIG. 4, the top surface of the first chamber 7 is serrated. The water injection nozzle LL is connected to the water absorption nozzle 9 via a spacer 15 at a position slightly above the tip of the water absorption nozzle 9.
The spacer 15 is held in place and opened to the outside through a slit 16 provided in the spacer 15.

17 is a pipe connecting the first chamber 7 and a water pump (not shown); 18 is a pipe connecting the second chamber 8 and a water suction pump (not shown)
It is a pipe that connects the

Reference numeral 19 denotes a holding plate that holds each tank 6 at the rear end, and this holding plate 19 is supported above the rail 20 to removably and fixedly support the entire plurality of tanks 6. is screwed onto the rail 20 via a support plate 21 and a ring 22, and can be moved up and down by rotating the rail 20 with the driving force of a motor 23. 24 and 25 are stoppers that determine the upper and lower limit positions of the table 2.

26, when the stand 2 is raised, the mouth of the test tube 3 is connected to the nozzle 5.
This guide plate 26 is a guide plate that guides the test tubes 3 to be located in the center, and this guide plate 26 has a hole with a trapezoidal cross section in which the lower base is larger than the test tube diameter and the upper base is smaller at the position corresponding to each test tube 3. has been done. Reference numeral 27 denotes a guide bar whose lower end is supported by the guide plate 26 and is loosely mounted on the holding plate 21.

〔effect〕

As described above, in the present invention, the inside of the tank is divided into a plurality of chambers, which are stacked one on top of the other, and a plurality of nozzles are provided in the chambers. The number of chambers is equal to that of the tank, making it lighter and smaller than conventional equipment. In addition, since the pipes are installed in each tank chamber, the length of the pipe from the water supply tank is the same, and the wave resistance is as equal as possible across all water injection channels, so differences in the amount of water injected from each nozzle can be ignored. This makes it possible to perform accurate quantitative water injection.

In addition, because multiple chambers are layered under one roof, and multiple nozzles are formed that communicate with each chamber, the entire area can be made compact, making maintenance such as operation, replacement, and cleaning easier. It will be easier to do.

Furthermore, as a result of using multiple nozzles, like parallel nozzles,
Since it spreads little in the lateral direction, it is easy to clean with water, and when inserting the nozzle, it prevents the test tube from coming into contact with the rim and damaging itself or the test tube, and it also prevents the nozzle from coming into contact with itself or the test tube. There is no fear that it will get clogged with deposits because there is no such thing.

[Brief explanation of the drawing]

The drawings show a liquid dispensing and absorbing device according to the present invention, and FIG. 1 is a partially sectional side view of the entire device. Figure 2 is an enlarged sectional view of the tank and nozzle, ff13 [1 is the water injection nozzle and the first
4 is a sectional view showing another embodiment of the top recess; FIG. 5 is an enlarged sectional view showing the connection state of the water injection nozzle and the water suction nozzle below; FIG. 6 is a perspective view of the spacer. 1...Liquid dispensing water absorption device, 3...Test tube 5...Nozzle 6...Tank 7...WSf chamber 8...Second chamber
9... Water absorption nozzle 10... Upper opening 11
...Water injection nozzle 12...Top surface 13...
Recess 14...Slit 15...Spacer 16
...slit F...pipe

Claims (1)

[Claims] It has a tank that is vertically layered with a chamber communicating with the water pump and a chamber communicating with the water suction pump, and a plurality of multiple nozzles are vertically installed below this tank, and each nozzle of the multiple nozzles is connected to each of the above chambers. A liquid dispensing and absorbing device characterized by being independently connected.