JPS63151860A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To remove influence of changes in the concn. of a test liquid to be reinspected, by correcting the concn. based on a measured value at the reinspection of reinspection-required items by a specified formula based on measured values as obtained separately from the initial inspection and reinspection of other items. CONSTITUTION:A command is provided to a sample feeder 4 and a reagent feeder 5 from a control section 8 and a liquid to be inspected prepared in a fixed sequence is conveyed by a reaction line 3 and optical density is measured sequentially with a photometer 6. The control section 8 is so operated as to reinspect required items and other items selected as desired in parallel therewith about a desired sample selected. A computing section 11 corrects the concn. A measured of the required items by a formula A=A.a/b (wherein A represents concn. of required items after correction) based on an initial inspection measured value (a) and an reinspection measured value (b) of those items requiring no reinspection. This removes effect of condensation due to evaporation which is unavoidably caused from the period of initial inspection to reinspection from the sample to be reinspected.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to an automatic analyzer. More specifically, the present invention relates to an automatic biochemical analyzer that uses biological fluids such as blood, serum, urine, etc. as samples, and is configured to be able to reexamine arbitrary items.

(B) Conventional technology Conventionally, automatic analyzers used in fields such as clinical biochemical testing generally have preset settings for each of a large number of specimens delivered in a fixed order under standard measurement conditions. A device is known that is configured to be able to retest any of the items on any of these specimens after passing the initial testing on a series of tested items. Such an apparatus is configured such that a re-examination is performed when a measurement value of a predetermined item of a certain specimen cannot be obtained normally in the initial examination due to the specimen or the apparatus.

(c) Problems to be solved by the invention Recent progress in automatic dispensing has been remarkable, and the amount of specimen collected has been miniaturized, contributing to reducing the burden on patients. This poses new problems in terms of handling liquids. In other words, the time it takes to complete a series of initial tests depends on the number of specimens and test items,
Depending on the type, it may take a very long time, and concentration of the test liquid before it can be retested becomes a problem. Since the evaporation of water from the surface of the test liquid is roughly proportional to the surface area, the smaller the amount of test liquid, the greater the degree of concentration, and this error cannot be ignored clinically.

The present invention has been made in view of the above circumstances, and particularly aims to provide an automatic analyzer having a function of compensating for the influence of concentration changes in test liquids to be retested.

(d) Means for Solving the Problems According to the present invention, the present invention includes a multi-item analysis section that measures a series of preset items for each of a large number of samples delivered in a certain order, An automatic biochemical analyzer configured to be able to retest any of the items on any of these samples after the initial test on a group of samples has been completed, and to determine which items require retesting on any selected sample A control unit that executes re-examination of other arbitrarily selected items in parallel, and concentration A based on the measured value at the time of re-examination of the above-mentioned items requiring re-examination,
Measured values a, b of the other items listed above at the initial inspection and re-examination
Based on the following formula: % formula % (In the formula, A' is the concentration of the item requiring re-examination after correction)
An automatic analyzer is provided that includes a calculation unit that corrects and outputs the influence of concentration due to evaporation that occurs from the time of the initial test to the time of the retest for a sample to be retested.

When retesting an item for which a normal measurement value cannot be obtained in the first test due to the specimen or the device, the device of the present invention also subjects the normally measured items of the specimen to the retest, and the results are as follows. Based on the obtained initial test measurement value and retest measurement value of the normal item, it is possible to calculate the retest measurement value that compensates for the concentration change dynamic component due to evaporation that occurs in the specimen that is left unattended from the initial test to the retest. It is characterized by being configured.

Enzyme activity items (
For example, CPK, GOT, GPT.

LD) I, etc.).

From a clinical standpoint, the items used for the above criteria are those with small concentration changes (small fluctuation range of normal values) and items with stable analytical accuracy. For example, electrolytes (
(Na", K", etc.), total protein, albumin, etc. These standard items may be specially set when setting the test items for the specimen, and if they are included in the items requested for testing at the time of the initial test, they may be specified. Good too.

In the arithmetic unit used in the apparatus of the present invention, compensation for the concentration change is performed based on proportional calculation. In other words, the initial measurement value (a) and re-examination measurement value (b) of the above standard items.
By multiplying the ratio (a/b) by the concentration (A) based on the measured value at the time of the re-examination of the item that requires re-examination, the above concentration (A) is the concentration based on the measured value at the initial inspection. It will be calibrated to

(E) Effect According to the present invention, when retesting an item that requires retesting for a given specimen, other items of the specimen are retested in parallel under the same conditions as the initial test, and the above results are obtained. The amount of change between the initial test measurement value and the retest measurement value for other items is used as a standard for measurement value fluctuations due to concentration due to evaporation that occurs in specimens left unattended from the initial test to the retest. The concentration based on the retest measurement value is calculated by compensating for the concentration without the influence of concentration.

The present invention will be described in detail below with reference to Examples, but the present invention is not limited thereby.

(F) Embodiment FIG. 1 is an explanatory diagram of the configuration of an embodiment of the apparatus of the present invention. In the figure, the biochemical automatic analyzer (1) includes a large number of reaction vessels (2)... and a reaction line (3) that allows these reaction vessels to be circulated in the direction of the arrow (A) by a drive unit (not shown). ), sampler (41) and sample dispenser (
42) and a reagent storage (not shown) equipped with a large number of reagents corresponding to the intended analysis items.
and a reagent and supply device (5) equipped with a reagent dispenser (51).
) and a flow cell (62) connected to a test liquid suction nozzle (61) by a flow cell (62) for multi-item analysis (6)
, a control section (with a built-in microcomputer) that controls the operation of this biochemical automatic analyzer (1) through a nozzle (7) for sucking and discharging the liquid in the reaction tube, and an input section (81) for inputting the operating procedures of each of the above-mentioned devices. 8), a recording section (9) that is electrically connected to the control section (8) and records the optical density measurement value of the test liquid measured by the photometric device (6), and a storage section (9) that stores the measured value. 10), and an arithmetic unit (11) which is electrically connected to the storage unit (10) and performs arithmetic processing based on a predetermined arithmetic operation from the measured values stored. Note that (12) is a cleaning device, (13) is a reaction container drying device, and (14) is a reaction tank capable of maintaining the measurement target sample in the reaction container at an intended temperature.

When starting an analysis using this automatic biochemical analyzer (1), the test order of a series of items and the test items required for each sample are determined in a fixed order. The sample supply order is set in advance in the input section (81).

After preparing a group of samples in the sampler (41), the control unit (8) instructs the sample supply device (4) and reagent supply device (5) to operate according to the input procedure described above, and supplies the sample liquid in a fixed order. Prepare. These test liquids are conveyed through the reaction line in a fixed order, and the optical density of each test liquid is sequentially measured by a photometer (6). These measured values are sequentially output to the recording section (9) and the storage section (10), where they are recorded and stored. In this way, the first test of a series of items on a group of specimens is performed. A group of specimens prepared in the sampler (41) is left in the sampler (41) until the series of initial tests are completed.

In the above initial test, if the measured value displayed in the recording section (9) is determined to require retesting, the relevant specimen and its items (hereinafter referred to as items requiring retesting) will be displayed in the input section (81) after the initial test. are set sequentially.

At this time, the input section (81) selects items that are determined to require no retesting from among the items that have already been measured for the sample (hereinafter referred to as items that do not require retesting), and selects them to be measured together with the items that require retesting. Set.

According to the setting procedure described above, the specimens designated for retesting and left on the sampler (41) are sequentially prepared into test liquids in the same manner as described above, and retesting operations are sequentially performed.

In this operation, both the items requiring retesting and the selected items not requiring retesting for one test liquid are measured by the photometer (6), and the results are stored in the storage unit (10).

Next, in the arithmetic unit (11), the measured concentration (A) of the item requiring retesting is determined by combining the already stored initial test measurement value (a) of the item not requiring retesting and the retest measurement value stored by this retesting operation. Based on the formula below; % formula %, the oil rx is output as -A°.Thus, the retest measurement values for items requiring retesting can be calculated while the values are left on the sampler (41) until the retest. , the effect of concentration due to evaporation that occurs in the sample is offset by comparing the initial test measurement value and retest measurement value of the same sample that has the same effect and does not require retesting, and the effect of concentration is corrected and output. will be done.

(G) Effects of the Invention According to the present invention, it is possible to eliminate the influence of changes in sample concentration due to retesting. Further, there is no need for special consideration or measures on the device for changes in the concentration of the specimen, and highly reliable retest measurement values can be easily obtained.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram of the configuration of an embodiment of the automatic analyzer of the present invention. (2)・・・・・・Reaction container, (3)・・・・・・
Reaction line, (4)... Sample supply device, (5)
...Reagent supply device, (6) ...Photometer, (7) ...Reaction tube internal liquid suction and discharge nozzle, (9
)... Recording Department, (10)...
Storage unit, (11)...Calculation unit, (41)
... Sampler, (42) ... Sample dispenser, (81) ... Eye... Input section.

Claims (1)

[Scope of Claims] 1. A multi-item analysis unit that measures a series of preset items for each of a large number of samples delivered in a fixed order, and after completing the initial test on a group of samples, An automatic biochemical analyzer configured to retest any of these items for any sample, including items that require retesting and other arbitrarily selected items for any selected sample. a control unit that executes re-inspection in parallel;
Measured values a, b of the other items listed above at the initial inspection and re-examination
Based on the following formula; A^1 = A x (a/b) (in the formula, A^1 is the concentration of the item that requires retesting after correction), which occurs from the time of the initial test to the time of the retest for the sample to be retested. An automatic analyzer comprising a calculation unit that corrects and outputs the influence of concentration due to evaporation.