JPS5863854A - Automatic chemical analyzing apparatus - Google Patents

Abstract

PURPOSE:To obtain an automatic analyzing apparatus in which reqiurements for labors, time and cost are reduced, in absorbance measurement for clinical examinations etc., by finding a characteristic of the secular change of a reagent under a fixed condition preliminarily and providing a means for making a calibration curve basing on said characteristic. CONSTITUTION:In the case where a characteristic of a reagent for absorbance measurement for an analysis of components in blood serum etc. is varied with the elapse of time after adjustment even when said reagent is kept at a constant low temperature, a tray 1 for keeping a vessel 1a containing the reagent at a constant low temperature and a tray 2 accommodating a sample vessel 2a in which a sample such as blood serum etc. is placed is kept at a constant temperature by means of a computer (CPU) 14, respectively and the absorbance increased or decreased with the time elapsed after adjusting the reagent preliminarily, is measured and is stored in the CPU14. Hereby, a calibration curve is made automatically at the measured date and hour by said data and the measurement is performed by reducing remarkably labors and time of the calibration of the apparatus etc.

Description

[Detailed description of the invention] The present invention relates to an automatic chemical analyzer.

Automatic chemical analyzers used in clinical tests, etc. mix the serum of the specimen to be analyzed (hereinafter also referred to as sample) with a reagent corresponding to the analysis item, react it, measure the light of this to determine the absorbance, and measure the absorbance in advance. This is an apparatus that automatically performs analysis by determining the concentration of a sample corresponding to the absorbance from a calibration curve determined from a reagent and a standard substance such as standard serum and K.

However, the reagents generally deteriorate even when stored at low temperatures of 2 to 8 degrees Celsius, so they are tested using standard serum at certain time intervals.
[+IJ must be created.

By the way, in the past, the preparation of such test lines was carried out every day, and standard serum was expensive and denatured even when stored in the refrigerator, so it had to be prepared immediately before use, making it labor-intensive and economical. The present invention was made based on the above circumstances, and it is necessary to create a calibration curve based on the aging characteristics of the reagent determined in advance under certain conditions. The object of the present invention is to provide an automatic chemical analysis device fft that can reduce the labor and economic burden required for device calibration.

First, the principle of determining a calibration curve based on the deterioration of a reagent over time determined under certain conditions will be explained. In general, if the storage temperature of a reagent changes, the deterioration characteristics, i.e., the absorbance of the reagent (hereinafter also referred to as the reagent blank value) or the reaction characteristics of the reagent will change, so the storage temperature of the reagent is kept at a constant low temperature (hereinafter referred to as the storage temperature). There is. Furthermore, in order to proceed with the chemical reaction between the reagent and the sample during analysis in the photometry section, the reagent is maintained at a predetermined reaction temperature. Based on these conditions, for example, the concentration of the component to be measured C1
If the time-dependent change in the reaction with respect to the standard serum is expressed as a change in absorbance, the reaction curve shown in FIG. 1 can be obtained. Furthermore, if the prepared reagent is stored at the storage temperature for a period of time and then the reaction is allowed to proceed at the same reaction temperature as described above, the reaction curve 12 shown in FIG. 1 can be obtained. The absorbance B shown in FIG. 1 is a reagent blank value, and the absorbance lt+Δ
B1 is the reagent blank value when stored at a constant temperature of '1'11M1, and the absorbance M is the total 1. It is the absorbance after reacting for a time, and the absorbance M+ΔM1 is the absorbance after reacting for a time t1 with a standard serum having a measurement component concentration C1 that has been stored for a time T1. And this reactive all.

As shown in Figure 2 from 12, the calibration curve 1.1 during reagent preparation.
Then, the calibration curve L2 after storage for 1 hour from the time of reagent preparation is obtained. This calibration curve IJl is shown below (1
), the calibration curve L2 can be expressed as in equation (2).

......(2) (However, X is the concentration of the sample, and E is the measured absorbance.) As is clear from equations (1) and (2) above, a certain period of time has elapsed since the time of reagent preparation. For example, every 12 hours, a standard material is prepared by mixing the same reagent as the absorbance increase/decrease (hereinafter also referred to as reagent absorbance increase/decrease) ΔBl... based on the absorbance at the time of reagent preparation. Increase or decrease in absorbance of standard serum (hereinafter also referred to as standard serum absorbance increase or decrease) 4
If M1...le is determined in advance, a calibration curve can be created based on these and the elapsed time from the time of reagent preparation. Further, the reagent absorbance increase/decrease ΔL3t...
If the standard surface 7H absorbance increase/decrease ΔMt...le increases or decreases in proportion to the elapsed time from the time of reagent preparation,
A test line can be created from each proportionality constant (hereinafter also referred to as a correction coefficient) and the elapsed time from the time of preparation. From now on
In the principle explained above, the clear entry is determined over time under certain conditions, and a test line is created using one or both of these and the elapsed time from the time of reagent preparation.

The automatic chemical analyzer if of the present invention will be explained below with reference to the drawings.

FIG. 6 is a schematic explanatory diagram of an automatic chemical analyzer for a disk IJ type, which is an embodiment of the present invention.

In the figure, 1 is a reagent tray in which reagent containers 1α containing various reagents are stored and the reagents are kept at a constant low temperature by an electronic cooling element (not shown), and 2 is a sample to be analyzed. For example, it is a sample tray in which sample containers 2α containing serum or the like (hereinafter referred to as samples) are housed, and each can be moved back and forth in the directions of arrows E and F in the figure through appropriate means. Above these, a nozzle block 6 is provided so as to be movable back and forth along a pair of guide rails 4, and one sampling nozzle 6α and two reagent nozzles 6h are provided on the nozzle block 6 so as to be movable up and down. It is being

A sampling pump 5 is located near this nozzle block 6.
and a reagent pump 6 are arranged so that a predetermined amount of the sample in the sample container 2cL and the reagent in the reagent container 1a are sucked into the sampling nozzle filter α and the reagent nozzle 3h.

At the right end of the guide rail 4, a plurality of reaction containers 7 into which the aspirated sample and reagent are dispensed are intermittently rotated in the direction G in FIG. A reaction tank 8 is arranged in which the dispensed reagent reacts with the sample while maintaining the temperature. A suction nozzle 10 is installed at the right end of the reaction tank 8 for sucking the sample and reagent dispensed into the reaction container 7 and reacted through the slit holder 9.

It communicates with a photometry section 11 that measures the photometry of the aspirated sample and reagent. In addition, photometry 1lls 11 and the reaction tank 8
The reaction temperature is maintained at a constant temperature by a constant temperature bath 12. A light source 11α and a detector 11b are built in the section 11 on the side 'yt', and a converter 13 for converting the electrical signal from the detector 11A into "/f)", /r) A CPU 14 for inputting the output from the converter, etc., and an operation panel 15 equipped with a numeric keypad (not shown) for inputting various data to the CI'U l 4 are provided.Here, the CPU 1
Function 4 will be explained. First, a predetermined test J[M is created according to the analysis item, so that bone increase/decrease Δ131...1
and standard surface/H absorbance increase/decrease mΔM1...-T1. Toga CP
o The calibration curve is automatically created by reading predetermined increase/decrease meters ΔB1, ΔMl, .1 from the memory device according to the elapsed time from the time of preparation of these reagents. You can calculate the concentration of the sample by doing this. Note that the fixed conditions regarding the holding temperature and the reaction temperature are faithfully reproduced by controlling an electronic cooling element (not shown) provided in the reagent tray 1 and a constant temperature bath 12 by the CPU 14. . In this embodiment, a calibration curve creation means for creating a calibration curve based on the change in absorbance of a reagent over time determined in advance under certain conditions is incorporated as a function of the CPU 14.

Next, the operation of the automatic chemical analyzer constructed in this way will be explained. First, instruction data corresponding to an analysis item is input to the CPU 14 via the operation panel 15, so that the reagent tray 1 and the sample tray 2 are moved in the direction of the sixth arrow E in the figure.

The reagent and sample corresponding to the analysis item are moved in the F direction, and the reagent and sample corresponding to the analysis item are sucked into the reagent nozzle 3b and the sampling nozzle 6a of the nozzle block B through the reagent pump 6 and sampling pump 5. is dispensed into the reaction vessel 7 in the reaction tank 8, and the reaction proceeds.

The reacted reagent sample is then sucked into the photometry section 11 through the xenon nozzle 10, the transmission data from the light source 11α is detected by the detector 1lbVC, and the photometry data is transmitted through the converter 13. CPU 14
K is input.

Then, the reagent absorbance increase/decrease ΔBx...1 and the standard serum absorbance increase/decrease Ail which correspond to the reagent used and correspond to the elapsed time ttr+ from the time of preparation of the used reagent
... is read out from the storage device, and based on this, calculations as shown in equation (2) above are performed to automatically create a calibration curve and calculate the concentration of the sample. Become. In this way, the device shown in this example automatically performs a test based on the elapsed time from the time of reagent preparation.
Since the lines are created, the labor and economic costs required for device calibration can be significantly reduced, and moreover,
A high analysis of lI& can be guaranteed.

In the above example, the case where the reagents are kept at a constant low temperature by the electronic cooling element provided in the reagent tray was explained, but the reagents are stored at low temperature in a cold storage placed outside the device u, and the appropriate amount is stored at the time of analysis. It is also possible to remove the reagents and make them available for use.

In addition, in the above embodiment, the reagent absorbance increase/decrease ΔB1...
・Increase/loss ΔMl in standard serum absorbance and standard serum absorbance ΔMl・・L are both determined over time, and a calibration curve is created based on both of these. However, the present invention is not limited to this. Only the increase/decrease ΔM1...1 is determined in advance, and the operator calculates the increase/decrease/decrease ΔB1...1 in reagent absorbance using the usual method at regular intervals, and creates a calibration curve based on both of these. It is also possible to do so. In particular, by doing this, it is possible to check for abnormalities even when the temperature of the cold storage where the reagents are stored rises due to a power outage or the like and the absorbance of the reagents changes unexpectedly over time. Furthermore, if there is drift in the device itself, it is possible to create a more accurate calibration curve by measuring a reagent blank value.

Also, depending on the reagent, only the absorbance of the reagent itself changes,
Some reactions do not deteriorate. In this case, it is also possible to create a calibration curve by storing only the reagent absorbance increase/decrease Δ13t...n in CI)U.

In addition, in the above embodiment, a case was explained in which a calibration curve is automatically created, but when applied to an apparatus that measures light using spectrophotometric intensity B1, the calibration curve f&1 that changes over time as shown in FIG. It is also possible to prepare a diagram drawn in advance and analyze the sample using a calibration curve corresponding to the elapsed time from the time of reagent preparation to the time of analysis in the diagram. In addition, a spectrophotometer can be used to input the absorbance of the reagent and the factor value (the ratio of the absorbance of the standard substance When using the reagent, the changes over time in the absorbance, J11, and factor value of the reagent are determined in advance, and the absorbance and factor value of the reagent corresponding to the elapsed time from the time of reagent preparation are calculated using the spectroscopic light. By inputting the appropriate pressure once, the generated test sample can be easily stored and pressed for analysis.

As is clear from the above explanation, in the automatic chemical analyzer of the present invention, it is possible to create a calibration curve based on the deterioration characteristics over time of a reagent determined in advance under certain conditions. This method has excellent effects such as being able to reduce the labor and economic burden required for calibrating the device.

[Brief Description of the Drawings] Fig. 1 is an explanatory diagram showing a reaction curve, Fig. 2 is an explanatory diagram showing a calibration curve, and Fig. 6 is a schematic diagram of a discrete type automatic chemical analyzer that is an embodiment of the present invention. It is an explanatory diagram. 1... Reagent tray, 2... Sample tray, 8
...Reaction tank, 11...Photometry section, 14...C
P.U.

Claims (1)

[Claims] In an automatic chemical analyzer, a sample to be analyzed is mixed with a reagent and reacted, and the sample is analyzed based on the absorbance obtained by photometry and a calibration curve corresponding to the analysis item. A calibration curve creation means is provided for creating a calibration curve based on changes in the properties of the reagent over time determined under the conditions, and based on the calibration curve creation means and the elapsed time from the time of adjustment of the reagent. An automatic chemical analyzer characterized by being capable of creating a calibration curve.