JPS63146782A - 高リパ−ゼ活性を有する菌体の生産方法 - Google Patents
高リパ−ゼ活性を有する菌体の生産方法Info
- Publication number
- JPS63146782A JPS63146782A JP61293754A JP29375486A JPS63146782A JP S63146782 A JPS63146782 A JP S63146782A JP 61293754 A JP61293754 A JP 61293754A JP 29375486 A JP29375486 A JP 29375486A JP S63146782 A JPS63146782 A JP S63146782A
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- lipase
- microorganisms
- retaining material
- holding material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000019626 lipase activity Nutrition 0.000 title claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 title claims description 20
- 230000000813 microbial effect Effects 0.000 title abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 80
- 239000000463 material Substances 0.000 claims abstract description 72
- 108090001060 Lipase Proteins 0.000 claims abstract description 63
- 102000004882 Lipase Human genes 0.000 claims abstract description 63
- 239000004367 Lipase Substances 0.000 claims abstract description 61
- 235000019421 lipase Nutrition 0.000 claims abstract description 61
- 241000235527 Rhizopus Species 0.000 claims abstract description 8
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 7
- 229930195729 fatty acid Natural products 0.000 claims abstract description 7
- 239000000194 fatty acid Substances 0.000 claims abstract description 7
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 7
- 125000005456 glyceride group Chemical group 0.000 claims abstract description 4
- 241000228212 Aspergillus Species 0.000 claims abstract description 3
- 241000235395 Mucor Species 0.000 claims abstract description 3
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 12
- 239000011148 porous material Substances 0.000 abstract description 6
- 235000008390 olive oil Nutrition 0.000 abstract description 5
- 239000004006 olive oil Substances 0.000 abstract description 5
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 abstract description 4
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 abstract description 4
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 abstract description 4
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 abstract description 4
- 239000005642 Oleic acid Substances 0.000 abstract description 4
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 abstract description 4
- -1 oleic acid Chemical class 0.000 abstract description 4
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 abstract description 4
- 239000012531 culture fluid Substances 0.000 abstract 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 description 25
- 238000005809 transesterification reaction Methods 0.000 description 21
- 230000000694 effects Effects 0.000 description 17
- 239000003925 fat Substances 0.000 description 10
- 235000019197 fats Nutrition 0.000 description 10
- 238000006460 hydrolysis reaction Methods 0.000 description 10
- 239000003921 oil Substances 0.000 description 9
- 235000019198 oils Nutrition 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 238000005273 aeration Methods 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 6
- 230000014759 maintenance of location Effects 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000009776 industrial production Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 125000001477 organic nitrogen group Chemical group 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000000411 inducer Substances 0.000 description 4
- 239000013028 medium composition Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 241000235528 Rhizopus microsporus var. chinensis Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
- HPEUJPJOZXNMSJ-UHFFFAOYSA-N Methyl stearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC HPEUJPJOZXNMSJ-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 241000588264 Rhizopus javanicus Species 0.000 description 2
- 244000205939 Rhizopus oligosporus Species 0.000 description 2
- 235000000471 Rhizopus oligosporus Nutrition 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- WDYVUKGVKRZQNM-UHFFFAOYSA-N 6-phosphonohexylphosphonic acid Chemical compound OP(O)(=O)CCCCCCP(O)(O)=O WDYVUKGVKRZQNM-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 101710158368 Extracellular lipase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000498617 Mucor javanicus Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- 241000235545 Rhizopus niveus Species 0.000 description 1
- 241001247145 Sebastes goodei Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 101710128940 Triacylglycerol lipase Proteins 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 210000001557 animal structure Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 150000001993 dienes Chemical class 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- CAMHHLOGFDZBBG-UHFFFAOYSA-N epoxidized methyl oleate Natural products CCCCCCCCC1OC1CCCCCCCC(=O)OC CAMHHLOGFDZBBG-UHFFFAOYSA-N 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000013310 margarine Nutrition 0.000 description 1
- 239000003264 margarine Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61293754A JPS63146782A (ja) | 1986-12-09 | 1986-12-09 | 高リパ−ゼ活性を有する菌体の生産方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61293754A JPS63146782A (ja) | 1986-12-09 | 1986-12-09 | 高リパ−ゼ活性を有する菌体の生産方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63146782A true JPS63146782A (ja) | 1988-06-18 |
| JPH0430833B2 JPH0430833B2 (enrdf_load_html_response) | 1992-05-22 |
Family
ID=17798793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61293754A Granted JPS63146782A (ja) | 1986-12-09 | 1986-12-09 | 高リパ−ゼ活性を有する菌体の生産方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63146782A (enrdf_load_html_response) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016073976A (ja) * | 2009-07-24 | 2016-05-12 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 攪拌システム |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS509868A (enrdf_load_html_response) * | 1973-06-04 | 1975-01-31 | ||
| JPS5088283A (enrdf_load_html_response) * | 1973-12-05 | 1975-07-15 |
-
1986
- 1986-12-09 JP JP61293754A patent/JPS63146782A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS509868A (enrdf_load_html_response) * | 1973-06-04 | 1975-01-31 | ||
| JPS5088283A (enrdf_load_html_response) * | 1973-12-05 | 1975-07-15 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016073976A (ja) * | 2009-07-24 | 2016-05-12 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 攪拌システム |
| JP2017209676A (ja) * | 2009-07-24 | 2017-11-30 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 攪拌システム |
| JP2021007946A (ja) * | 2009-07-24 | 2021-01-28 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 攪拌システム |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0430833B2 (enrdf_load_html_response) | 1992-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ellaiah et al. | Production of lipase by immobilized cells of Aspergillus niger | |
| Karube et al. | Continous hydrogen production by immobilized whole cells of Clostridium butyricum | |
| Sankpal et al. | Optimization of fermentation conditions for gluconic acid production using Aspergillus niger immobilized on cellulose microfibrils | |
| Nakashima et al. | Culture conditions for intracellular lipase production by Rhizopus chinensis and its immobilization within biomass support particles | |
| Wisdom et al. | Enzymic interesterification of fats: Laboratory and pilot‐scale studies with immobilized lipase from Rhizopus arrhizus | |
| Bayramoğlu et al. | Covalent immobilization of lipase onto hydrophobic group incorporated poly (2-hydroxyethyl methacrylate) based hydrophilic membrane matrix | |
| FI68078B (fi) | Foerfarande foer framstaellning av biologiskt aktiva mikroorganismmyceliepellets | |
| Kokubu et al. | α-Amylase production by immobilized whole cells of Bacillus subtilis | |
| Elibol et al. | Lipase production by immobilised Rhizopus arrhizus | |
| Lee et al. | Citric acid production by Aspergillus niger immobilized on polyurethane foam | |
| Christen et al. | Microbial lipase production on a polymeric resin | |
| Koshcheyenko et al. | Immobilization of living microbial cells and their application for steroid transformations | |
| Yong et al. | Kinetic studies on immobilised lipase esterification of oleic acid and octanol | |
| AÇIKEL et al. | The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar | |
| JP2787015B2 (ja) | 油脂資化性細菌およびそれを使用した油脂の処理方法 | |
| Park et al. | Itaconic acid production using an air-lift bioreactor in repeated batch culture of Aspergillus terreus | |
| Chen et al. | Enzymatic hydrolysis of triglycerides by Rhizopus delemar immobilized on biomass support particles | |
| JPH0543354B2 (enrdf_load_html_response) | ||
| JPS63146782A (ja) | 高リパ−ゼ活性を有する菌体の生産方法 | |
| Warmuth et al. | Selection of a support for immobilization of a microbial lipase for the hydrolysis of triglycerides | |
| Elegado et al. | Screening, identification and optimization of extracellular lipase production of yeast (Cryptococcus flavescens) isolated from a tree canopy fern in the Mount Makiling Forest Reserve, Philippines | |
| Darah et al. | Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells of Phanerochoete chrysosporium in a tubular air-lift bioreactor | |
| JPS61209596A (ja) | 固定化微生物による有機酸の製法 | |
| JPH01225487A (ja) | セルロースのアスペルギルス ニガーによるクエン酸若しくはグルコン酸生産を目的としたバイオリアクター担体への利用方法 | |
| Lopez et al. | Lipolytic enzyme production by immobilized Rhizopus oryzae |