JPS6312312A - Electric field ion exchange chromatography - Google Patents
Electric field ion exchange chromatographyInfo
- Publication number
- JPS6312312A JPS6312312A JP61156141A JP15614186A JPS6312312A JP S6312312 A JPS6312312 A JP S6312312A JP 61156141 A JP61156141 A JP 61156141A JP 15614186 A JP15614186 A JP 15614186A JP S6312312 A JPS6312312 A JP S6312312A
- Authority
- JP
- Japan
- Prior art keywords
- ion
- exchange membrane
- water
- substance
- equifield
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005684 electric field Effects 0.000 title claims abstract description 13
- 238000004255 ion exchange chromatography Methods 0.000 title description 3
- 239000000126 substance Substances 0.000 claims abstract description 24
- 239000003014 ion exchange membrane Substances 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 11
- 238000002347 injection Methods 0.000 abstract description 4
- 239000007924 injection Substances 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 3
- 150000002500 ions Chemical class 0.000 description 10
- 229910052751 metal Inorganic materials 0.000 description 7
- 239000002184 metal Substances 0.000 description 7
- 239000002253 acid Substances 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 4
- 229960000907 methylthioninium chloride Drugs 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000003411 electrode reaction Methods 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229910052770 Uranium Inorganic materials 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- JFALSRSLKYAFGM-UHFFFAOYSA-N uranium(0) Chemical compound [U] JFALSRSLKYAFGM-UHFFFAOYSA-N 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【発明の詳細な説明】
この発明は、直流電源あるいは両極性パルス電源を平行
金属板電極につなぐ事によって等電界を発生させ、その
等電界中のイオン交換膜あるいは極性基の配列に一定の
方向性を持った物に水溶性極性物質を吸着せしめ、その
後、電界の大きさや極性等を変えることによって脱離溶
出させる、水溶性物質の分離精製方法に関するものであ
る。Detailed Description of the Invention This invention generates an equal electric field by connecting a DC power supply or a bipolar pulse power supply to parallel metal plate electrodes, and the arrangement of the ion exchange membrane or polar groups in the equal electric field has a fixed direction. This invention relates to a method for separating and purifying water-soluble substances, in which a water-soluble polar substance is adsorbed onto a substance having a polarity, and then desorbed and eluted by changing the magnitude and polarity of an electric field.
従来よりイオン交換膜はその選択的な
イオン３看過↑ノ１によって電−（透析法、拡散透析法
及び電解法に利用８れている。 主として食ＩＭ製造と
か、食」ｎ電解にＪ、るり「１ル・）ノル力り製造技術
として使用されている。 このようにイオン交換体の選
択的イオン透過↑１は広範囲に利用されているが、吸着
やｊ脱着による分ＦＡ１粕製には使用されていない。Conventionally, ion exchange membranes have been used in electrolytic dialysis, diffusion dialysis, and electrolytic methods due to their selective ionization.They are mainly used in food IM production and food electrolysis. The selective ion permeation ↑1 of ion exchangers is used in a wide range of applications, but it is used in the production of FA1 lees through adsorption and desorption. It has not been.
−・般に水溶性物７１の分離箱；伎にはイオン交換樹脂
、セル「］−ス・イオン交換体、セファデックス・イオ
ン交換体等のイオン交換体によってｉ１′なわれている
。−こＵらイオン交換体による分離精製は酸、ＪＷ塁、
あるいは塩の水溶液を通し、接触さけることによって吸
６及σ１１り離溶出さＵることにより？）なわれるもの
である。 これら酸、１ｎ塁及び塩水溶液の取扱いはか
なり危険な仕手であり、これらは金属、その配管等を腐
蝕させるのでその取扱いには注意を要する、又、強酸必
るいは強Ｊ福塁溶液中では不安定な物′１″！１もあり
、分離粘製が困難な場合もある。 このようにイオン交
換体による分Ｆ４精製には多大の労力と日数及び経験が
一般に必要とされる。- Generally, a separation box for water-soluble substances 71; in this case, it is made up of an ion exchanger such as an ion exchange resin, a cell ion exchanger, a Sephadex ion exchanger, etc. Separation and purification using U et al. ion exchanger is acid, JW base,
Or by passing the salt through an aqueous solution and avoiding contact with it to absorb and elute it? ). Handling these acids, 1N base and salt aqueous solutions is quite dangerous, and care must be taken when handling them as they corrode metals and their piping. There are some substances '1''!1 that are unstable, and separation and viscosity production may be difficult.As described above, purification of fraction F4 using an ion exchanger generally requires a great deal of effort, days, and experience.
本発明を使用すれば誰でも容易に短時間の内に水溶性物
質の分離精製を行なうことが出来る。By using the present invention, anyone can easily separate and purify water-soluble substances within a short time.
それを図面について説明すれば、第１図に示すように、
タンク１から溶媒としての水を定量ポンプ２で汲みあげ
て一定量ずつカラム・セル３中に流しておき、カラム・
セル中の２枚の平行金属電極板４に両極性高圧パルス電
源５から直流あるいは両極性パルス電圧を加えることに
よってその平行金属電極板の間のイオン交換膜６を含む
領域に等電界を発生させる、注入ロアより分離精製しよ
うとする物質を注入し、等電界が作用しているイオン交
換膜にその物質を吸着させる、その後等電界をＯにし、
不純物を洗い流す、続いて、電界の大きざを連続勾配か
不連続勾配で変えたり、極性やパルスの周期を変えるこ
とにより、その物質をイオン交換膜から脱臼１さぜる、
この様にして分離精製した物Ｙ’ｆの濃度変化を検出器
８で測定し、記録する。 検出器としてはＵＶ−検出器
又は化学計測における電導度計を使用する。To explain this in terms of drawings, as shown in Figure 1,
Water as a solvent is pumped up from tank 1 using metering pump 2 and flows into the column cell 3 in fixed amounts.
Injection, in which a DC or bipolar pulse voltage is applied from a bipolar high-voltage pulse power source 5 to two parallel metal electrode plates 4 in the cell to generate an equal electric field in the region including the ion exchange membrane 6 between the parallel metal electrode plates. The substance to be separated and purified is injected from the lower part, and the substance is adsorbed on the ion exchange membrane where an isoelectric field is applied.Then, the isoelectric field is set to O.
Wash away impurities, then dislocate the material from the ion exchange membrane by varying the magnitude of the electric field with a continuous or discontinuous gradient, and by varying the polarity and pulse period.
Changes in the concentration of the product Y'f separated and purified in this manner are measured by the detector 8 and recorded. As a detector, a UV detector or a conductivity meter in chemical measurement is used.
両極性高圧パルス電源に関しては最近のパワー・エレク
１−［１ニクス（スイッチング素子としてのパワー・Ｆ
ＥＴ等）の発展によって容易に製作することができる。Regarding bipolar high-voltage pulse power supplies, recent power electronics 1-[1nics (power F as a switching element)
With the development of ET, etc.), it can be easily manufactured.
イオン交換体はその粒子の表層部分にイオン交換基がラ
ンダムに色々な方向に分イ［シているため、電界による
物質の吸着や脱着は困難であるが、イオン交１’ＡＦｓ
はａ９い膜であるため、その極性括（スルホンｖ％、第
４級アンモニウム阜等）がある方向性を持つことになり
、電界による極性物質の吸、脱着が可能になっていると
考えられる。 この様にイオン交換体によるイオン交換
クロマミルグラフィにおいては吸脱ル物質として酸、塩
基、塩水溶液を使用し、連続濃度勾配あるいはバッチ法
による不連続濃度勾配液を調製して分離精製を行なうが
、電界イオン交換クロマトグラフィにおいては電界によ
って物質の吸脱着を行なうので、誰でも容易に短時間で
分離精製することができるし、電気機器装置による機械
化及びコンピュータ制御も容易である。Ion exchangers have ion exchange groups randomly distributed in various directions on the surface of their particles, making it difficult to adsorb or desorb substances using an electric field.
Since it is a thin film, it has a certain directionality due to its polarity (sulfone v%, quaternary ammonium, etc.), and it is thought that it is possible to adsorb and desorb polar substances by an electric field. . In this way, in ion-exchange chromamilography using an ion exchanger, acids, bases, and salt aqueous solutions are used as adsorbing substances, and separation and purification is carried out by preparing a continuous concentration gradient solution or a discontinuous concentration gradient solution using a batch method. In electric field ion exchange chromatography, substances are adsorbed and desorbed by an electric field, so anyone can easily separate and purify the substance in a short time, and it is easy to mechanize using electrical equipment and control by computer.
電界イオン交換クロマミルグラフィにおいては溶媒とし
て比抵抗１０〜１００ｃｍ の水（水道水、蒸溜水
、純水等）を使用することができるので電極反応による
ラジカル等の妨害物質の発生は非常に少ないし、流れる
電流も非常に小さいので熱の発生も少なく、平行金属板
電極に高電圧をかけることができる。 又、この高電圧
も両極性矩形波パルス等の交流電源を使うことができる
ため、電極反応による妨害物質の発生を少なくする事が
できる。 この様に電界イオン交換クロマトグラフィは
妨害となる電極反応を出来るだけ少なくする様に調節す
ることが出来る。In electric field ion exchange chromamyllography, water (tap water, distilled water, pure water, etc.) with a specific resistance of 10 to 100 cm can be used as a solvent, so the generation of interfering substances such as radicals due to electrode reactions is extremely small. Since the current that flows is very small, little heat is generated, and a high voltage can be applied to the parallel metal plate electrodes. Furthermore, since an alternating current power source such as a bipolar square wave pulse can be used for this high voltage, it is possible to reduce the generation of interfering substances due to electrode reactions. In this way, field ion exchange chromatography can be adjusted to minimize interfering electrode reactions.
［実施例１
強Ｊｎ林性染料塩酸Ｊｎであるメチレンブルー＼ａの分
離１１’Ｊ”１４について実施した。[Example 1 Separation 11'J''14 of methylene blue \a, which is a strong Jn forest dye hydrochloric acid Jn, was carried out.
図２１こ示すようにカラム・ヒル３はポリアクリル仮装
の内容積が幅９ｍｍ、横４５ｍｍ、縦１００ｍｍの直方
体の容器である。 この中に２枚の平行金属板電４へ４
（厚さ°０，３ｍｍのステンレス Ｍ、 Ｉ［１，）を
平行に入れる、電（へ間の距離は７ｍｍである、電（〜
の各々の一喘にネジを取り付【プ、それに両４〜性パル
ス電源５からの電線を繋ぐ、そしてその２枚の平行金属
板電極の間に、イオン交換膜６、セレミオンＣ，Ｍ、Ｖ
、（旭硝子、陽イオン交換膜Ｎａ型）を平行に等間隔に
入れ、これら電）へとイオン交換膜をこの容器に封入し
たものをカラム・セルとして以下の実験に使用した。
カラム・ヒルに繋いでいるチ］ブを通して溶媒として１
００ＣｒＴｌ の水を常時、流速Ｑ、 ５ｒＴ１１
、／’ｍｉ ｎの速さで流しておく。As shown in FIG. 21, the column hill 3 is a rectangular parallelepiped container made of polyacrylic masquerade and having an internal volume of 9 mm in width, 45 mm in width, and 100 mm in length. In this, there are two parallel metal plate electrodes 4 to 4.
(Stainless steel M, I [1,
Attach a screw to each pane of the ion exchange membrane 6, Selemion C, M, V
(Asahi Glass Co., Ltd., Na-type cation exchange membranes) were placed in parallel at equal intervals, and the ion exchange membranes were sealed in this container, which was used as a column cell in the following experiments.
1 as a solvent through the tube connected to the column hill.
00CrTl water at all times, flow rate Q, 5rT11
, /'min speed.
次にその電極間に直流電圧６０Ｖをかける（電流３０ｍ
Ａ程度）。 次に粗製メチレンブルー溶液を注入ロアよ
り注入し、そのまま２分間程流してイオン交換膜に吸着
させる。 その後電圧をＯＶにもどし、しばらく水を流
して不純物を洗い流してしまう。 それから、両極性矩
形波パルス電圧（＋側のパルス幅周期３秒、−側のパル
ス幅周期２０秒）をかけ、そのパルス電圧を徐々に上げ
ていく、±６０Ｖ程度に上げる時、メチレンブルーが溶
出してくるので、これを集めて精製メチレンブルーとし
た。Next, apply a DC voltage of 60 V between the electrodes (current of 30 m
Grade A). Next, a crude methylene blue solution is injected from the injection lower and allowed to flow for about 2 minutes to be adsorbed onto the ion exchange membrane. After that, the voltage is returned to OV and water is run for a while to wash away impurities. Then, a bipolar rectangular wave pulse voltage (pulse width period of 3 seconds on the + side, pulse width period of 20 seconds on the - side) is applied, and the pulse voltage is gradually increased. When raised to about ±60V, methylene blue is eluted. This was collected and used as purified methylene blue.
本発明の効果として誰でも容易に短時間で高度な水溶性
物質の分離精製を行なうことができる点にあると考えら
れる。It is believed that one of the effects of the present invention is that anyone can easily perform high-level separation and purification of water-soluble substances in a short period of time.
遺伝子工学関連のバイオテクノロジーにおいて蛋白質等
の水溶性物質の分離精製がしばしば行われているが、非
常に労力と時間を要するので、問題となっている３、
ここに本発明を！；６１１ｊ Ｖる１１１史に一層の発
ＩＩ５か明ｊ！ｔされる。Separation and purification of water-soluble substances such as proteins is often carried out in biotechnology related to genetic engineering, but this is a problem because it requires a lot of effort and time.
The invention is here! ;611j Vru 111 history has a further development II 5 or clear j! t will be done.
ｔｌｏ水にはウランか無限に含まれていると占われてい
る。 現在、ｙ−タン酸やイオン交換（か１脂ににるｉ
／＋ｊ水からのウラン回収実験が行われているが、その
操作の繁雑さ、回収率の低さ故に、いまだ十分な結果は
得られていない。 ここに“し本発明を応用することが
できると考えられる。It is predicted that the tlo water contains an infinite amount of uranium. Currently, y-tanic acid and ion exchange (or
/+j Experiments to recover uranium from water have been conducted, but sufficient results have not yet been obtained due to the complexity of the operations and low recovery rate. It is believed that the present invention can be applied here.
第１図は本発明の実施の一例を示ず系統図である。
第２図は本発明の実施の一例を示り“部分断面斜視図で
必る。 １は中に水の入ったタンク ２は定量ポンプ
３はカラム・セル ４は平１１金属板電（〜 ５は両極
性高圧パルス電源（Ｏ〜±１６０Ｖ） ６はイオン交
４６Ａ膜 ７は注入口 ８は検出蔦（化学計測に一引プ
る電尊磨計）
特、；４出願人 公１１ζ泰浩
第４図
（Ｏ〜ヨ１６０Ｖ）
５ 雨曝性高圧パルス電源
（Ｏ〜±１６０Ｖ）FIG. 1 is a system diagram showing an example of the implementation of the present invention. Figure 2 shows an example of the implementation of the present invention and is a partially sectional perspective view. 1 is a tank with water inside, 2 is a metering pump.
3 is a column cell 4 is a flat metal plate electrode (~ 5 is a bipolar high voltage pulse power supply (O~±160V) 6 is an ion exchanger 46A membrane 7 is an injection port 8 is a detection tube (a voltage that is useful for chemical measurement) 4 Applicant: Yasuhiro Public 11ζ Figure 4 (O~YO 160V) 5 Rain-exposed high voltage pulse power supply (O~±160V)
Claims (1)
つた物に等電界をかけることによつて物質を吸着及び脱
着溶出させる分離精製を行なう方法。A separation and purification method in which substances are adsorbed, desorbed, and eluted by applying an equal electric field to an ion exchange membrane or other material that has a certain directionality in the arrangement of polar groups.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61156141A JPS6312312A (en) | 1986-07-04 | 1986-07-04 | Electric field ion exchange chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61156141A JPS6312312A (en) | 1986-07-04 | 1986-07-04 | Electric field ion exchange chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6312312A true JPS6312312A (en) | 1988-01-19 |
Family
ID=15621219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61156141A Pending JPS6312312A (en) | 1986-07-04 | 1986-07-04 | Electric field ion exchange chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6312312A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0394299A (en) * | 1989-05-17 | 1991-04-19 | American Teleph & Telegr Co <Att> | Voice recognition method and method of training of voice recognition apparatus |
| JP2007526110A (en) * | 2003-12-09 | 2007-09-13 | セパレーション デザイン グループ、エルエルシー | Sorption methods, equipment, and systems |
-
1986
- 1986-07-04 JP JP61156141A patent/JPS6312312A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0394299A (en) * | 1989-05-17 | 1991-04-19 | American Teleph & Telegr Co <Att> | Voice recognition method and method of training of voice recognition apparatus |
| JP2007526110A (en) * | 2003-12-09 | 2007-09-13 | セパレーション デザイン グループ、エルエルシー | Sorption methods, equipment, and systems |
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