JPS63119671A - Cell fusion device - Google Patents
Cell fusion deviceInfo
- Publication number
- JPS63119671A JPS63119671A JP61263892A JP26389286A JPS63119671A JP S63119671 A JPS63119671 A JP S63119671A JP 61263892 A JP61263892 A JP 61263892A JP 26389286 A JP26389286 A JP 26389286A JP S63119671 A JPS63119671 A JP S63119671A
- Authority
- JP
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- Prior art keywords
- cells
- electrode
- outer peripheral
- cell fusion
- fusion device
- Prior art date
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- Pending
Links
- 230000007910 cell fusion Effects 0.000 title claims abstract description 24
- 230000002093 peripheral effect Effects 0.000 claims abstract description 16
- 238000004720 dielectrophoresis Methods 0.000 claims description 32
- 239000006059 cover glass Substances 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 9
- 230000004927 fusion Effects 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims 1
- 238000013508 migration Methods 0.000 abstract description 3
- 230000005012 migration Effects 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 42
- 238000000034 method Methods 0.000 description 6
- 230000005684 electric field Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 2
- 101100394497 Caenorhabditis elegans toe-1 gene Proteins 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003989 dielectric material Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Electromagnetism (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
この発明は、電磁波の刺激により細胞融合を引き起こす
細胞融合装置に関し、特に、融合させたい２種類の細胞
を確実に接触させて融合することのできる細胞融合装置
に関するものである。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a cell fusion device that causes cell fusion by stimulation of electromagnetic waves, and particularly relates to a cell fusion device that causes cell fusion by stimulating two types of cells to be fused. This relates to a cell fusion device that can be used.
［従来の技術］
従来、この種の装置を用いて細胞結合を引き起・１・
極
こす方法としては、微少電流法、平行電極法及び誘電泳
動チェンバ（ｄｉｅｌｅｃｔｏｐｈｏｒｅｓｉｓ ｃｈ
ａｍｂｅｒ）法が提案されている。この中で、誘電泳動
チェンバ法は、特に細胞の操作が容易で大量処理が可能
なため、既にこの原理を利用した装置が何種類が市販さ
れている。[Prior art] Conventionally, this type of device has been used to induce cell binding.
Microcurrent methods, parallel electrode methods, and dielectrophoresis chamber methods are available.
amber) method has been proposed. Among these, the dielectrophoresis chamber method is particularly easy to manipulate cells and allows for large-scale processing, and several types of devices using this principle are already on the market.
に
第５図は、例えば「細胞工学Ｊ（Ｖｏｌ、３、ＮＯ，儀
、１９８４）の第５００頁に記載された、従来の誘電泳
動チェンバ法に用いられる細胞融合装置を一部ブロック
国で示す平面図である。Figure 5 shows, for example, a cell fusion device used in the conventional dielectrophoresis chamber method described in page 500 of "Cell Engineering J (Vol. 3, NO. Gi, 1984)" in some block countries. FIG.
図において、（１）は数１００ｋＨｚ〜数ＭＨｚの高周
波（ＲＦ）信号を発生する発振器である。（２）は発振
器（１）に接続され、持続時間が数１０μ秒のパルス信
号を発生するパルス発生器であり、後述する２種類の細
胞を融合させるための融合手段となっている。In the figure, (1) is an oscillator that generates a radio frequency (RF) signal of several 100 kHz to several MHz. (2) is a pulse generator that is connected to the oscillator (1) and generates a pulse signal with a duration of several tens of microseconds, and serves as a fusion means for fusing two types of cells, which will be described later.
（３）は後述するスライドガラス（４）及びカバーガラ
ス（５）で形成される誘電泳動チェンバ、（４）はスラ
イドガラス、（５）はスライドガラス（４）上に設けら
れたカバーガラスである。（６）及び（７）はスライド
ガラス（４）とカバーガラス（５）との間（誘電泳動チ
ェンバ（３）内）に平行に配置された一対の電極であり
、これらは発振器（１）及びパルス発生器（２）に接続
されている。(3) is a dielectrophoresis chamber formed by a slide glass (4) and a cover glass (5) which will be described later, (4) is a slide glass, and (5) is a cover glass provided on the slide glass (4). . (6) and (7) are a pair of electrodes arranged in parallel between the slide glass (4) and the cover glass (5) (inside the dielectrophoresis chamber (3)), and these are the oscillator (1) and Connected to a pulse generator (2).
（８）は発振器（１）及びパルス発生器（２）に接続さ
れ、発振波形やパルス波形をモニタするためのオシロス
コープである。(8) is an oscilloscope connected to the oscillator (1) and the pulse generator (2) for monitoring oscillation waveforms and pulse waveforms.
次に、第５図に示した従来の細胞融合装置の動作につい
て説明する。Next, the operation of the conventional cell fusion device shown in FIG. 5 will be explained.
まず、融合させたい２種類の細胞を含む懸濁液をスライ
ドガラス（４）とカバーガラス（５）との間に注入する
。First, a suspension containing two types of cells to be fused is injected between a slide glass (4) and a cover glass (5).
続いて、発振器（１）から数１００ｋＨｚ〜数ＭＨｚの
交流電圧を発生し、一対の電極（６）及び（７）を介し
て懸濁液中に交流電界を発生させる。この交流電界に基
づく誘電泳動により、一対の電極（６）、〈７）間に細
胞が数珠状に並び、いわゆるパールチェーンが形成され
る。Subsequently, an AC voltage of several 100 kHz to several MHz is generated from the oscillator (1), and an AC electric field is generated in the suspension via the pair of electrodes (6) and (7). Due to dielectrophoresis based on this alternating electric field, cells are arranged in a beaded pattern between the pair of electrodes (6) and <7), forming a so-called pearl chain.
その後、パルス発生器（２）からパルス信号を発生して
２種類の細胞を融合させ、所望の融合細胞を得る。Thereafter, a pulse signal is generated from a pulse generator (2) to fuse the two types of cells to obtain a desired fused cell.
［発明が解決しようとする問題点］
従来の細胞融合装置は以上のように、一対の平行電極（
６）、（７）間で細胞を融合させているので、異種の細
胞が１個ずつ融合したものの他に、融合しなかったもの
、同種の細胞が融合したもの又は３個以上の細胞が融合
したものなど種々の細胞が生成されて選別が困難であり
、又、異種の細胞を１個ずつ誘電泳動チェンバ（３）内
に注入しても、２種類の細胞が希望通り接触せず、所望
の融合細胞を確実に生成することができないという問題
点があった。[Problems to be solved by the invention] As described above, the conventional cell fusion device uses a pair of parallel electrodes (
Since cells are fused between 6) and (7), in addition to cells of different types fused one by one, cells that did not fuse, cells of the same type fused, or three or more cells fused It is difficult to sort cells because various types of cells are generated, and even when cells of different types are injected one by one into the dielectrophoresis chamber (3), the two types of cells do not come into contact with each other as desired. There was a problem in that it was not possible to reliably generate fused cells.
この発明は上記のような問題点を解決するためになされ
たもので、２種類の細胞を確実に接触させて融合し、所
望の融合細胞を生成することのできる細胞融合装置を得
ることを目的とする。This invention was made to solve the above-mentioned problems, and the purpose is to obtain a cell fusion device that can generate desired fused cells by bringing two types of cells into contact with each other and fusing them. shall be.
［問題点を解決するための手段］
この発明に係る細胞融合装置は、ほぼ円筒形状をなす誘
電泳動チェンバの外周部に沿って設けられた外周電極と
、誘電泳動チェンバの中心部に設けられた中心電極と、
各電極間に高周波信号を印加して２種類の細胞を中心電
極付近に集中させるための発振器と、２種類の細胞を融
合させるための融合手段とを備えたものである。[Means for Solving the Problems] The cell fusion device according to the present invention includes an outer peripheral electrode provided along the outer periphery of a dielectrophoresis chamber having a substantially cylindrical shape, and an electrode provided at the center of the dielectrophoresis chamber. a center electrode;
It is equipped with an oscillator for applying a high frequency signal between each electrode to concentrate two types of cells near the center electrode, and a fusion means for fusing the two types of cells.
［作用］
この発明においては、高周波信号を印加して２種類の細
胞を中心電極付近に集中させ、接触した細胞同士を融合
手段により融合させる。[Operation] In the present invention, two types of cells are concentrated near the center electrode by applying a high frequency signal, and the cells in contact are fused with each other by the fusion means.
［実施例］
以下、この発明の一実施例を図について説明する。第１
図はこの発明の一実施例を一部ブロック図で示す側面図
、第２図は第１図の誘電泳動チェンバを示す平面図であ
り、（１）、（２）及び（８）は前述の従来装置と同様
のものである。[Example] Hereinafter, an example of the present invention will be described with reference to the drawings. 1st
The figure is a side view showing a partial block diagram of an embodiment of the present invention, FIG. 2 is a plan view showing the dielectrophoresis chamber of FIG. 1, and (1), (2), and (8) are This is similar to the conventional device.
（１４）はガラスなどの誘電体で構成された円筒形状の
誘電泳動チェンバである。 （１６）は誘電泳動チェン
バ（１４）の外周面外側に沿って形成され円筒形状をな
した外周電極、（１７）は誘電泳動チェンバ（１４）の
中心部に設けられた中心電極であり、それぞれ発振器（
１）及びパルス発生器（２）に接続されている。（１８
）及び（１９）は誘電泳動チェンバ（１４）内に注入さ
れた２種類の細胞である。(14) is a cylindrical dielectrophoresis chamber made of a dielectric material such as glass. (16) is a cylindrical outer peripheral electrode formed along the outside of the outer peripheral surface of the dielectrophoresis chamber (14), and (17) is a center electrode provided at the center of the dielectrophoresis chamber (14). Oscillator (
1) and a pulse generator (2). (18
) and (19) are two types of cells injected into the dielectrophoresis chamber (14).
次に、第１図及び第２図に示したこの発明の一実施例の
動作について説明する。Next, the operation of the embodiment of the present invention shown in FIGS. 1 and 2 will be explained.
まず、融合対象となる細胞の浸透圧に適合した溶液を誘
電泳動チェンバ（１４）内に満たしておく。First, the dielectrophoresis chamber (14) is filled with a solution that matches the osmotic pressure of the cells to be fused.
この溶液は、例えば植物プロトプラストを融合する場合
は、０．７Ｍマニトール溶液＋１（ｌｓＭｃａイオンな
どである。This solution is, for example, 0.7 M mannitol solution + 1 (lsMca ion, etc.) when fusing plant protoplasts.
続いて、融合対象となる２種類の細胞（１８）及び（１
９）を１個ずつ誘電泳動チェンバ（１４）内に注入し、
更に、発振器（１）から高周波信号を発生させて、外周
電極（１６）と中心電極（１７）との間に数１００ｋＨ
ｚ〜数ＭＨｚの交流電圧を印加する。この交流電圧によ
り誘電泳動チェンバ（１４）内には、第２図の破線で示
すような放射方向の交流電界の電気力線が発生する。こ
のような交流電界内にある誘電体は誘電泳動により電界
密度の強い方に力を受けるので、各細胞（１８）及び（
１９）は矢印方向に力を受けて誘電泳動チェンバ（１４
）の中心部即ち中心電極〈１７）に向かって移動し、中
心電極（１７）付近に集中して互いに接触する。Next, two types of cells to be fused (18) and (1
9) into the dielectrophoresis chamber (14) one by one,
Furthermore, a high frequency signal is generated from the oscillator (1) to generate a signal of several 100 kHz between the outer electrode (16) and the center electrode (17).
An alternating current voltage of several MHz to several MHz is applied. This alternating current voltage generates lines of electric force of an alternating electric field in the radial direction in the dielectrophoresis chamber (14) as shown by broken lines in FIG. A dielectric substance in such an alternating electric field receives force in the direction of the stronger electric field density due to dielectrophoresis, so each cell (18) and (
19) receives a force in the direction of the arrow and moves into the dielectrophoresis chamber (14).
), that is, the center electrode (17), and are concentrated near the center electrode (17) and come into contact with each other.
この状態で、パルス発生器（２）を駆動して外周電極（
１６）、中心電極（１７）間にパルス信号を印加すると
、各細胞（１日）及び（１９）は細胞融合を起こして１
つの融合細胞となる。In this state, drive the pulse generator (2) to drive the outer peripheral electrode (
16), when a pulse signal is applied between the center electrodes (17), each cell (1 day) and (19) undergo cell fusion and become 1
They become two fused cells.
こうして、高周波信号により２種類の細胞（１８）及び
（１９）を中心電極（１７）付近に集中させた後、パル
ス信号によって融きさせ、所望の融合細胞を確実に生成
する。In this way, the two types of cells (18) and (19) are concentrated near the center electrode (17) by the high-frequency signal, and then melted by the pulse signal to reliably generate the desired fused cells.
尚、上記実施例では誘電泳動チェンバ（１４）を−体で
構成したが、第３図に示すように、スライドグラス（４
）に形成された貫通穴（４ａ）と、この貫通穴（４ａ）
の底部に設けられたカバーガラス（５）とにより構成し
てもよい、この場合、外周電極り１６）は貫通穴（４ａ
）の外周上面に設けられ、中心電極（１７）はカバーガ
ラス（５）の中心部の下面に設けられる。Incidentally, in the above embodiment, the dielectrophoresis chamber (14) was composed of a glass slide, but as shown in FIG.
) and this through hole (4a).
In this case, the outer circumferential electrode plate 16) may be configured with a cover glass (5) provided at the bottom of the through hole (4a
), and the center electrode (17) is provided on the lower surface of the center of the cover glass (5).
又、円筒形状の外周電極（１６）を一体で構成したが、
第４図に示すように、円周方向に分割された複数（例え
ば４個）の部分電極（２０）〜（２３）で構成してもよ
い、この場合、各部分電極（２０）〜（２３）に印加す
る信号を選択的に制御するためのコントローラ（２４）
が、各部分電極（２０）〜（２３）と発振器（１）及び
パルス発生器（２）との間に設けられている。In addition, although the cylindrical outer peripheral electrode (16) was integrally constructed,
As shown in FIG. 4, it may be composed of a plurality of (for example, four) partial electrodes (20) to (23) divided in the circumferential direction. In this case, each partial electrode (20) to (23) ) a controller (24) for selectively controlling the signal applied to the
are provided between each partial electrode (20) to (23) and the oscillator (1) and pulse generator (2).
第４図の実施例の場合、誘電泳動チェンバ（１４）内に
注入された細胞（図示せず）を中心電極（１７）付近に
集中させるときは、各部分電極（２０）〜（２３）を同
電位として、中心電極（１７）との間に数１００ｋＨｚ
〜数ＭＨｚの交流信号を印加する。そして、各細胞が接
触した後は、接触方向に応じて各部分電極（２０）〜（
２３）に選択的にパルス信号を印加する０例えば、各細
胞が第４図の縦方向に並んだときは、部分電極（２１）
及び（２２）を同電位に、又、部分電極（２０）及び（
２３）を同電位にして、これら部分電極対間にパルス信
号を印加する。このように、接触した細胞膜に垂直に電
圧を印加することにより、細胞融合作用は極めて効率良
く行なわれる。In the case of the embodiment shown in FIG. 4, when cells (not shown) injected into the dielectrophoresis chamber (14) are concentrated near the center electrode (17), each partial electrode (20) to (23) is Several 100kHz between the center electrode (17) and the same potential.
Apply an AC signal of ~ several MHz. After each cell comes into contact, each partial electrode (20) to (
For example, when each cell is lined up in the vertical direction in Fig. 4, the partial electrode (21)
and (22) at the same potential, and partial electrodes (20) and (
23) are set at the same potential, and a pulse signal is applied between the pair of partial electrodes. In this way, by applying a voltage perpendicularly to the cell membranes in contact, the cell fusion effect is carried out extremely efficiently.
更に、上記各実施例では、２種類の細胞を融合させるた
めの融合手段として、高電圧のパルス信号を発生するパ
ルス発生器（２）を用いたが、レーザを照射するレーザ
照射手段を用いてもよい、この場合、各細胞が中心電極
（１７）付近に必ず集中して接触領域が限定されている
ので、レーザ照射点の設定も極めて容易となる。Furthermore, in each of the above examples, a pulse generator (2) that generates a high voltage pulse signal is used as a fusion means for fusing two types of cells, but a laser irradiation means that irradiates a laser is used. In this case, since each cell is necessarily concentrated near the center electrode (17) and the contact area is limited, it is extremely easy to set the laser irradiation point.
［発明の効果］
以上のようにこの発明によれば、２種類の細胞が注入さ
れる誘電泳動チェンバをほぼ円筒形状に構成し、この誘
電泳動チェンバの外周部に沿って外周電極を設けると共
に、誘電泳動チェンバの中心部に中心電極を設け、各電
極間に高周波信号を印加して中心電極付近に集中された
２種類の細胞を接触させて融合するようにしたので、所
望の融合細胞を確実に生成できる細胞融合装置が得られ
る効果がある。[Effects of the Invention] As described above, according to the present invention, the dielectrophoresis chamber into which two types of cells are injected is configured in a substantially cylindrical shape, and an outer peripheral electrode is provided along the outer circumference of the dielectrophoresis chamber, and A center electrode is provided at the center of the dielectrophoresis chamber, and a high-frequency signal is applied between each electrode to bring the two types of cells concentrated near the center electrode into contact and fuse, ensuring the desired fused cells. This has the effect of providing a cell fusion device that can be produced in a number of ways.
第１図はこの発明の一実施例を一部ブロック図で示す側
面図、第２図は第１図の誘電泳動チェンバを示す平面図
、第３図はこの発明の第２の実施例の誘電泳動チェンバ
を示す側面図、第４図はこの発明の第３の実施例を一部
ブロック図で示す平面図、第５図は従来の細胞融合装置
を一部ブロック図示す平面図である。
（１）・・・発振器 （２）・・・パルス発生
器（４）・・・スライドグラス （４ａ）・・・貫通穴
（５）・・・カバーガラス
（１４）・・・誘電泳動チェンバ
（１６）・・・外周電極 （１７）・・・中心電
極（１８）、（１９）・・・２種類の細胞（２０）〜（
２３）・・・部分電極 （２４）・・・コントローラ尚
、図中、同一符号は同−又は相当部分を示す。
１４： 誘電帰ｌカテ二″−ｔｆ
１６： 外周電極
１７： 中Ｉｌｌ’電極
１８．１９ ： ２裡類ｊ臼吃
４ ： スフづトウ１゛フス
４ａ ： ｉｊ通た
５ ： 力／１”−７）ｌ’ウス
２０〜２３．９分ｔ々
篤５図FIG. 1 is a side view showing a partial block diagram of an embodiment of the present invention, FIG. 2 is a plan view showing the dielectrophoresis chamber of FIG. 1, and FIG. FIG. 4 is a side view showing a migration chamber, FIG. 4 is a plan view partially showing a block diagram of a third embodiment of the present invention, and FIG. 5 is a plan view partially showing a block diagram of a conventional cell fusion device. (1)...Oscillator (2)...Pulse generator (4)...Slide glass (4a)...Through hole (5)...Cover glass (14)...Dielectrophoresis chamber ( 16)...Outer electrode (17)...Center electrode (18), (19)...Two types of cells (20)~(
23)... Partial electrode (24)... Controller In the drawings, the same reference numerals indicate the same or corresponding parts. 14: Dielectric return l category 2''-tf 16: Outer circumferential electrode 17: Middle Ill' electrode 18.19: 2nd class j press 4: Suffix toe 1゛fuss 4a: ij passed 5: Force/1''- 7) L'Us 20-23.9 minutes 5 figures
Claims (7)
電泳動チェンバと、この誘電泳動チェンバの外周部に沿
って設けられた外周電極と、前記誘電泳動チェンバの中
心部に設けられた中心電極と、前記各電極間に高周波信
号を印加して前記２種類の細胞を前記中心電極付近に集
中させるための発振器と、前記２種類の細胞を融合させ
るための融合手段とを備えた細胞融合装置。(1) A dielectrophoresis chamber that is approximately cylindrical in shape and into which two types of cells are injected, an outer peripheral electrode provided along the outer periphery of the dielectrophoresis chamber, and a center electrode provided at the center of the dielectrophoresis chamber. Cell fusion comprising an electrode, an oscillator for applying a high frequency signal between each of the electrodes to concentrate the two types of cells near the center electrode, and a fusion means for fusing the two types of cells. Device.
って設けられ円筒形状をなしたことを特徴とする特許請
求の範囲第１項記載の細胞融合装置。(2) The cell fusion device according to claim 1, wherein the outer peripheral electrode is provided along the outside of the outer peripheral surface of the dielectrophoresis chamber and has a cylindrical shape.
た貫通穴と、この貫通穴の底部に設けられたカバーガラ
スとからなり、外周電極は前記貫通穴の外周上面に設け
られ、中心電極は前記カバーガラスの下面に設けられた
ことを特徴とする特許請求の範囲第１項記載の細胞融合
装置。(3) The dielectrophoresis chamber consists of a through hole formed in a slide glass and a cover glass provided at the bottom of the through hole, an outer peripheral electrode is provided on the upper surface of the outer periphery of the through hole, and a center electrode is provided on the upper surface of the outer periphery of the through hole. The cell fusion device according to claim 1, wherein the cell fusion device is provided on the lower surface of a cover glass.
極から構成されたことを特徴とする特許請求の範囲第１
項乃至第３項のいずれかに記載の細胞融合装置。(4) Claim 1, characterized in that the outer peripheral electrode is composed of a plurality of partial electrodes divided in the circumferential direction.
The cell fusion device according to any one of items 1 to 3.
信号を印加するためのコントローラを有することを特徴
とする特許請求の範囲第４項記載の細胞融合装置。(5) The cell fusion device according to claim 4, wherein the fusion means includes a controller for selectively applying pulse signals between the plurality of partial electrodes.
信号を印加するためのパルス発生器であることを特徴と
する特許請求の範囲第１項乃至第４項のいずれかに記載
の細胞融合装置。(6) The fusion means is a pulse generator for applying a pulse signal between the outer peripheral electrode and the center electrode, according to any one of claims 1 to 4. Cell fusion device.
を照射するためのレーザ照射手段であることを特徴とす
る特許請求の範囲第１項乃至第４項のいずれかに記載の
細胞融合装置。(7) The cell fusion device according to any one of claims 1 to 4, wherein the fusion means is a laser irradiation means for irradiating a laser to the center of the dielectrophoresis chamber. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61263892A JPS63119671A (en) | 1986-11-07 | 1986-11-07 | Cell fusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61263892A JPS63119671A (en) | 1986-11-07 | 1986-11-07 | Cell fusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63119671A true JPS63119671A (en) | 1988-05-24 |
Family
ID=17395705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61263892A Pending JPS63119671A (en) | 1986-11-07 | 1986-11-07 | Cell fusion device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63119671A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001067084A1 (en) * | 2000-03-09 | 2001-09-13 | Matsushita Seiko Co., Ltd. | Method of measuring biological activity and device therefor |
| JP2006518847A (en) * | 2003-02-12 | 2006-08-17 | イギリス国 | Particle assembly device |
| JP2011223988A (en) * | 2010-03-31 | 2011-11-10 | Central Res Inst Of Electric Power Ind | Fermentation treatment apparatus |
-
1986
- 1986-11-07 JP JP61263892A patent/JPS63119671A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001067084A1 (en) * | 2000-03-09 | 2001-09-13 | Matsushita Seiko Co., Ltd. | Method of measuring biological activity and device therefor |
| JP2006518847A (en) * | 2003-02-12 | 2006-08-17 | イギリス国 | Particle assembly device |
| JP2011223988A (en) * | 2010-03-31 | 2011-11-10 | Central Res Inst Of Electric Power Ind | Fermentation treatment apparatus |
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