JPS63106541A - Integral absorbancy measuring apparatus - Google Patents
Integral absorbancy measuring apparatusInfo
- Publication number
- JPS63106541A JPS63106541A JP25236486A JP25236486A JPS63106541A JP S63106541 A JPS63106541 A JP S63106541A JP 25236486 A JP25236486 A JP 25236486A JP 25236486 A JP25236486 A JP 25236486A JP S63106541 A JPS63106541 A JP S63106541A
- Authority
- JP
- Japan
- Prior art keywords
- absorbance
- height
- absorbancy
- sample
- sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002835 absorbance Methods 0.000 claims description 19
- 238000011481 absorbance measurement Methods 0.000 claims description 7
- 238000005259 measurement Methods 0.000 abstract description 3
- 102100027340 Slit homolog 2 protein Human genes 0.000 abstract description 2
- 101710133576 Slit homolog 2 protein Proteins 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract 1
- 230000004304 visual acuity Effects 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 101700004678 SLIT3 Proteins 0.000 description 1
- 102100027339 Slit homolog 3 protein Human genes 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明は、混合溶液の沈澱の影響を無視出来る吸光度
計測装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an absorbance measuring device that can ignore the influence of precipitation of a mixed solution.
この発明は吸光度計測装置において、ライン光源、一次
元ラインセンサーを採用することにより混合物の沈澱速
度の差によって生じる吸光度の誤差を無視出来るように
したものである。The present invention employs a line light source and a one-dimensional line sensor in an absorbance measuring device, thereby making it possible to ignore errors in absorbance caused by differences in sedimentation rates of mixtures.
従来、吸光度測定において図２に示すように、点光源か
ら発せられた光を試料容器の一部に照射し、つまり全長
にわたっては照射していない。その部分からの情報は同
図布に示したようなグラフからも理解出来るように試料
の一部からの情報でしかない。Conventionally, in absorbance measurement, as shown in FIG. 2, light emitted from a point light source is irradiated onto a part of a sample container, that is, the entire length is not irradiated. The information from that part is only information from a part of the sample, as can be understood from the graph shown in the same figure.
しかし、従来の吸光度計測では、溶媒中に混合している
物質が時間と共に沈澱する様な場合定点計測であるが故
に吸光度に誤差が生ずる。この欠点を補なう為に被計測
溶液が入っている試料容器全長からの吸光度を（試料容
器各々の高さに対する吸光度）ライン光源と一次元ライ
ンセンサーで構成する光学系で計測することによりその
積分吸光度を計測することを目的とする。However, in conventional absorbance measurement, if a substance mixed in a solvent precipitates over time, an error occurs in the absorbance because it is a fixed point measurement. In order to compensate for this drawback, the absorbance from the entire length of the sample container containing the solution to be measured (absorbance relative to the height of each sample container) is measured using an optical system consisting of a line light source and a one-dimensional line sensor. The purpose is to measure the integrated absorbance.
上記問題点を解決するために、この発明は試料容器全長
にわたり照射出来うるライン光源を採用し、試料全長よ
り吸光された光強度を？！数個の画素（チャネル）をｉ
Ｔするラインセンサーを検出器として配置し各高さより
の吸光度をラインセンサーにおいてその高さに対応する
画素（チャネル）に入力し、結果は各チャネルの吸光度
を積分することにより、試料容器全体からの吸光度を計
測するようにした。In order to solve the above problems, this invention employs a line light source that can irradiate the entire length of the sample container, and calculates the intensity of light absorbed from the entire length of the sample. ! Several pixels (channels) are
A line sensor is placed as a detector, and the absorbance from each height is input to the pixel (channel) corresponding to that height on the line sensor.The result is calculated from the entire sample container by integrating the absorbance of each channel. The absorbance was measured.
上記のように構成された積分型吸光度計測装置は溶液中
に存在する沈澱する物質が時間経過に伴う吸光度の変化
に対する誤差を著しく減少さゼることが出来るのである
。The integral type absorbance measurement device configured as described above can significantly reduce errors caused by changes in absorbance caused by precipitated substances present in a solution over time.
以下に、この発明の実施例を、凹面に基づいて説明する
。この積分型吸光度計測の原理は、Ｍ３に示す、試料に
照射する光強度を１゜、混合溶液が入っている試料容器
の厚さをＸ、試料にて吸収された後の光強度を１とする
と、
１ ＝ Ｉ ｏ ＊＊＊ （−ｐ ｘ） −一一一−−
（ｔｌが成立する。た＼このＩは試＄１全長のある部分
からの情報でしかない。それは１．で表わされる。Examples of the present invention will be described below based on concave surfaces. The principle of this integral absorbance measurement is as shown in M3, where the light intensity irradiating the sample is 1°, the thickness of the sample container containing the mixed solution is X, and the light intensity after being absorbed by the sample is 1. Then, 1 = I o *** (-p x) -111--
(tl holds true. This I is only information from a certain part of the total length of trial $1. It is expressed as 1.
もしラインセンサーの各画素の高さを１１とし、総画素
数をＮ１試料容器の高さをＨとすると、各々の画素に対
する吸光度は測定高さ分解能はｈで規制され、
Ｉ、＝Ｉ。、。（、μｘ） −−（２）でそれらの積分
吸光度（Ｉ、）は
１、＝Σ１．−−−（３）
にて求められる。この積分型吸光度計測装置はこの１１
を各々計測し最終的にＩｒ’Ｃ演算算出するものである
。第１図においてライン光源１から発する光を試料形状
によって異なるスリット中を有する入射型スリット２を
通過して試料容器３に照１１する。試料容器３の高さに
対応するラインセンサー５が検出器側スリット４の後方
に配置されている。ラインセンサ・−５は複数個の画素
（チャネル）より構成されており、その画素の１つの高
さが分解能で試料容器の各高さからの吸光度を計測する
ことが出来る。If the height of each pixel of the line sensor is 11, the total number of pixels is N1, and the height of the sample container is H, then the measurement height resolution of the absorbance for each pixel is regulated by h, I, = I. ,. (, μx) --(2) and their integrated absorbance (I,) is 1, = Σ1. --- It is determined by (3). This integral type absorbance measuring device is this 11
, respectively, and finally calculates the Ir'C calculation. In FIG. 1, light emitted from a line light source 1 passes through an incident type slit 2, which has different slits depending on the shape of the sample, and illuminates a sample container 3 (11). A line sensor 5 corresponding to the height of the sample container 3 is arranged behind the detector side slit 4. The line sensor -5 is composed of a plurality of pixels (channels), and the resolution is one height of the pixel, and the absorbance from each height of the sample container can be measured.
以」二述べた様に本発明によれば、試料容器の全体から
の吸光度を一度に測定することができ、試１４の容器内
に濃度分布が出来る場合においても誤差の極めて少ない
吸光度測定が出来るものであり分光分野においてその１
す用価値は、極めて大である。As described above, according to the present invention, it is possible to measure the absorbance of the entire sample container at once, and even when there is a concentration distribution within the container as in Test 14, absorbance measurement can be performed with extremely little error. No. 1 in the field of spectroscopy
The utility value is extremely large.
第１図は、本発明における積分型吸光度測定装置の概念
図である。第２図は、従来型の吸光度計装置の概念図で
ある。第３図は、未発明の原理を説明する図である。
１・・・ライン光源 ２・・・入射型スリット３・・・
試料容器 ４・・・受光型スリット５・・・一次元ラ
インセンサー
以上
積分型り反光崖ヲ則足に遷１迂他・
第１図
ず
１ｉ−１ｏｔ
芝来型吸え贋計裟置イ唾か
弔２図
！ｘＰ（−ＪＸ−）
５原Ｔ里説σ月図
第３図FIG. 1 is a conceptual diagram of an integral type absorbance measuring device according to the present invention. FIG. 2 is a conceptual diagram of a conventional absorbance meter device. FIG. 3 is a diagram illustrating the uninvented principle. 1... Line light source 2... Incident type slit 3...
Sample container 4... Light-receiving type slit 5... One-dimensional line sensor or more, integral type, reflection cliff, etc. Funeral diagram 2! xP(-JX-) 5 Hara Tri Theory σ Moon Map Figure 3
Claims (2)
た後の光強度を試料全長にわたり一次元ラインセンサー
にて吸光度を測定する積分型吸光度計測装置。(1) An integral type absorbance measurement device that irradiates a sample with a line light source and measures the absorbance of the light intensity after being absorbed by the sample using a one-dimensional line sensor over the entire length of the sample.
位置での透過光の強度を測定出来る特許請求の範囲第１
項記載の積分型吸光度計測装置。(2) The one-dimensional line sensor is capable of measuring the intensity of transmitted light at any height position of the sample.
The integral type absorbance measurement device described in .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25236486A JPS63106541A (en) | 1986-10-23 | 1986-10-23 | Integral absorbancy measuring apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25236486A JPS63106541A (en) | 1986-10-23 | 1986-10-23 | Integral absorbancy measuring apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63106541A true JPS63106541A (en) | 1988-05-11 |
Family
ID=17236265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25236486A Pending JPS63106541A (en) | 1986-10-23 | 1986-10-23 | Integral absorbancy measuring apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63106541A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0551430A1 (en) * | 1990-10-03 | 1993-07-21 | Abbott Laboratories | Fabrication of multifunctional holographic optical elements and application in photometers |
| EP3974838A3 (en) * | 2009-08-13 | 2022-08-03 | Siemens Healthcare Diagnostics Inc. | Methods and apparatus for ascertaining interferents and physical dimensions in liquid samples and containers to be analyzed by a clinical analyzer |
-
1986
- 1986-10-23 JP JP25236486A patent/JPS63106541A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0551430A1 (en) * | 1990-10-03 | 1993-07-21 | Abbott Laboratories | Fabrication of multifunctional holographic optical elements and application in photometers |
| EP0551430A4 (en) * | 1990-10-03 | 1994-08-03 | Abbott Laboratories | |
| EP3974838A3 (en) * | 2009-08-13 | 2022-08-03 | Siemens Healthcare Diagnostics Inc. | Methods and apparatus for ascertaining interferents and physical dimensions in liquid samples and containers to be analyzed by a clinical analyzer |
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